Featured
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Brief Communication
| Open Access
A monomeric StayGold fluorescent protein
The bright, photostable fluorescent protein StayGold is converted into a monomer.
- Esther Ivorra-Molla
- , Dipayan Akhuli
- & Mohan K. Balasubramanian
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News & Views |
Brain imaging turned inside out
Neural connectivity in brain tissue is imaged by labeling the extracellular space.
- Shahrzad Askari
- & Thomas Misgeld
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News & Views |
Imaging glycosylated RNAs at the subcellular scale
A recently discovered RNA species on the cell surface is studied by proximity ligation.
- Petar Hristov
- & Ryan A. Flynn
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Article
| Open Access
Imaging brain tissue architecture across millimeter to nanometer scales
Mapping fixed brain samples with extracellular labeling and optical microscopy reveals synaptic connections.
- Julia M. Michalska
- , Julia Lyudchik
- & Johann G. Danzl
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Brief Communication
| Open Access
Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity
A dual illumination method reduces photobleaching for green and yellow fluorescent proteins.
- Lucie Ludvikova
- , Emma Simon
- & Agathe Espagne
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Research Briefing |
Breaking boundaries in whole-body imaging and disease understanding with wildDISCO
Wild three-dimensional imaging of solvent-cleared organs (wildDISCO) uses conventional antibodies for whole-body staining in mice, creating comprehensive biological atlases of nerves, blood vessels and lymphatics. It uncovers pathological changes, such as tertiary lymphoid structures in cancer, and it enables the precise tracking of therapeutic molecules and cells, enhancing our understanding of disease pathology and treatment.
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Article
| Open Access
Whole-body cellular mapping in mouse using standard IgG antibodies
Whole mice are immunolabeled with standard antibodies using wildDISCO.
- Hongcheng Mai
- , Jie Luo
- & Ali Ertürk
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Article |
Spatial imaging of glycoRNA in single cells with ARPLA
GlycoRNA imaging in cells is enabled with a proximity ligation assay.
- Yuan Ma
- , Weijie Guo
- & Yi Lu
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Research Briefing |
Imaging the biological microcosmos with a tiny telescope
We present the Schmidt objective, a novel astronomy-inspired concept for designing multi-immersion microscope objectives with high numerical aperture, long working distance and large field of view. The Schmidt objective uses a spherical mirror and a correction plate to focus light. It is well suited for high-resolution imaging deep inside cleared biological samples.
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Article
| Open Access
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope
Imaging of large, cleared samples in diverse media is achieved using a mirror objective.
- Fabian F. Voigt
- , Anna Maria Reuss
- & Fritjof Helmchen
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Research Briefing |
Imaging large cell populations with fast, automated super-resolution
Leveraging advances in hardware and probes, we have combined innovations in optics and algorithms to allow automated single-molecule-based super-resolution imaging at unprecedented throughput. This approach allows us to obtain nanoscale information from large cell populations, bridging the gap between imaging and indirect ensemble methods.
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Article |
An integrated platform for high-throughput nanoscopy
A fast data processing platform enables super-resolution microscopy with increased throughput.
- Andrew E. S. Barentine
- , Yu Lin
- & David Baddeley
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Brief Communication |
Trans-segmental imaging in the spinal cord of behaving mice
A wearable macroscope enables cellular imaging across multiple spinal segments in behaving mice.
- Pavel Shekhtmeyster
- , Daniela Duarte
- & Axel Nimmerjahn
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News & Views |
Writing cellular history in protein chains
Elongating protein fibers record the transcriptional activity of single cells for later readout by imaging.
- Hyung-Bae Kwon
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Article
| Open Access
Real-time, volumetric imaging of radiation dose delivery deep into the liver during cancer treatment
Radiation dose delivery is measured in real time for a patient with cancer.
- Wei Zhang
- , Ibrahim Oraiqat
- & Issam El Naqa
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Article |
A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments
A sensor for oxytocin reveals mechanisms of oxytocin action with high spatiotemporal precision.
- Tongrui Qian
- , Huan Wang
- & Yulong Li
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Article
| Open Access
Magnify is a universal molecular anchoring strategy for expansion microscopy
Expansion microscopy with gel-anchored molecules enables simultaneous retention of nucleic acids, proteins and lipids in diverse tissue types.
- Aleksandra Klimas
- , Brendan R. Gallagher
- & Yongxin Zhao
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Article
| Open Access
Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria
Gene expression is imaged in deep tissues in mice using ultrasound.
- Robert C. Hurt
- , Marjorie T. Buss
- & Mikhail G. Shapiro
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Article
| Open Access
Recording of cellular physiological histories along optically readable self-assembling protein chains
A history of cellular events is recorded in self-assembling protein chains.
- Changyang Linghu
- , Bobae An
- & Edward S. Boyden
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Article
| Open Access
Real-time denoising enables high-sensitivity fluorescence time-lapse imaging beyond the shot-noise limit
DeepCAD-RT denoises fluorescence time-lapse images in real time.
- Xinyang Li
- , Yixin Li
- & Qionghai Dai
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Article
| Open Access
Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH
EEL FISH profiles spatial transcriptomes in single cells in large, complex tissue samples.
- Lars E. Borm
- , Alejandro Mossi Albiach
- & Sten Linnarsson
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Research Briefing |
Visualization of living cells and tissues in many colors
Existing technologies can map the intricate spatial distribution of biomolecules and cell types within tissues, but not in specimens that remain alive and intact. This study introduces scission-accelerated fluorophore exchange (SAFE) bioorthogonal imaging tools that enable living cells and tissues to be deeply and serially profiled, revealing their biological dynamics across both space and time.
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Article |
Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes
Live cells and tissues are imaged over long time periods using a fast, non-toxic click chemistry.
- Jina Ko
- , Martin Wilkovitsch
- & Jonathan C. T. Carlson
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Research Briefing |
Sustainable bioimaging using a fluorescent protein with unprecedented photostability
The jellyfish-derived green fluorescent protein StayGold is bright and hardly fades, contributing to improving spatiotemporal resolution and dramatically extending the observation period. To fully benefit from the rich photon budget, we tried some unusual illumination modalities for sustainable, quantitative live-cell or volumetric imaging.
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Article
| Open Access
A highly photostable and bright green fluorescent protein
StayGold is over one order of magnitude more photostable than current fluorescent proteins
- Masahiko Hirano
- , Ryoko Ando
- & Atsushi Miyawaki
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Article |
Computationally designed dual-color MRI reporters for noninvasive imaging of transgene expression
MRI detection of transgene expression in animals is expanded to two colors.
- Hyla Allouche-Arnon
- , Olga Khersonsky
- & Amnon Bar-Shir
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Article
| Open Access
Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging
Calcium and other analytes can be imaged at super-resolution and in vivo with photo-switchable sensors.
- Kanuj Mishra
- , Juan Pablo Fuenzalida-Werner
- & Andre C. Stiel
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Article |
Whole-cell segmentation of tissue images with human-level performance using large-scale data annotation and deep learning
Deep learning algorithms perform as well as humans in identifying cells in tissue images.
- Noah F. Greenwald
- , Geneva Miller
- & David Van Valen
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Article |
A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo
A genetically encoded sensor reveals the dynamics of endocannabinoid signaling.
- Ao Dong
- , Kaikai He
- & Yulong Li
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News & Views |
Light-sheet microscopy at high resolution
An improved light-sheet microscope images live cells at sub-100-nm axial resolution.
- Reto Fiolka
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Article |
Volumetric interferometric lattice light-sheet imaging
New lattice light-sheet microscopy approach improves live cell imaging.
- Bin Cao
- , Simao Coelho
- & Alexandros Pertsinidis
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Article |
Highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing
Simultaneous, Raman-based imaging of more than 10 proteins in thick samples.
- Lixue Shi
- , Mian Wei
- & Wei Min
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Article |
High-throughput mapping of a whole rhesus monkey brain at micrometer resolution
A whole monkey brain is imaged at high resolution in 100 hours.
- Fang Xu
- , Yan Shen
- & Guo-Qiang Bi
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Article |
Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D
An imaging software for large-scale microscopy data shows how cells are organized in tissues.
- Ravian L. van Ineveld
- , Michiel Kleinnijenhuis
- & Anne C. Rios
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Letter |
Compressed sensing for highly efficient imaging transcriptomics
Spatial transcriptomics is enhanced by using imaging probes for subsets of genes.
- Brian Cleary
- , Brooke Simonton
- & Aviv Regev
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Brief Communication |
Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics
A light-up RNA aptamer enables single-molecule localization microscopy with a resolution not limited by photobleaching.
- Murat Sunbul
- , Jens Lackner
- & Andres Jäschke
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Brief Communication |
Left-handed DNA-PAINT for improved super-resolution imaging in the nucleus
Left-handed DNA probes improve super-resolution DNA-PAINT imaging in the nucleus by reducing background.
- H. J. Geertsema
- , G. Aimola
- & H. Ewers
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Article |
A near-infrared genetically encoded calcium indicator for in vivo imaging
A near-infrared fluorescent calcium indicator can be combined with other optogenetic tools in vivo without spectral crosstalk.
- Anton A. Shemetov
- , Mikhail V. Monakhov
- & Vladislav V. Verkhusha
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Article |
Imaging volumetric dynamics at high speed in mouse and zebrafish brain with confocal light field microscopy
A new imaging method enables deep imaging of neural networks.
- Zhenkun Zhang
- , Lu Bai
- & Kai Wang
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Article |
Rapid image deconvolution and multiview fusion for optical microscopy
Microscopy datasets are processed orders-of-magnitude faster with improved algorithms and deep learning.
- Min Guo
- , Yue Li
- & Hari Shroff
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Brief Communication |
Plants with genetically encoded autoluminescence
Luminescence is engineered in whole plants, without an exogenous substrate, using a fungal gene cluster.
- Tatiana Mitiouchkina
- , Alexander S. Mishin
- & Karen S. Sarkisyan
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Article |
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
The spatial location and sequence of DNA barcodes are detected with high sensitivity in fixed tissues.
- Amjad Askary
- , Luis Sanchez-Guardado
- & Michael B. Elowitz
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Article |
Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues
A DNA-based amplification scheme enables highly multiplexed immunofluorescence imaging
- Sinem K. Saka
- , Yu Wang
- & Peng Yin
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News & Views |
Brain maps at the nanoscale
Large brain tissue sections are imaged with nanoscale resolution using expansion and lattice light sheet microscopy.
- Weijian Yang
- & Rafael Yuste
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Article |
Protection of tissue physicochemical properties using polyfunctional crosslinkers
Controlled tissue fixation with polyepoxides protects protein fluorescence, antigenicity, nucleic acids and tissue architecture.
- Young-Gyun Park
- , Chang Ho Sohn
- & Kwanghun Chung
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Letter |
ClampFISH detects individual nucleic acid molecules using click chemistry–based amplification
Sensitive detection of individual RNA and DNA molecules is achieved by exponentially amplifying the fluorescence signal.
- Sara H Rouhanifard
- , Ian A Mellis
- & Arjun Raj
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Article |
A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies
- Miao Jing
- , Peng Zhang
- & Yulong Li
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Article |
Deep learning massively accelerates super-resolution localization microscopy
Accelerating PALM/STORM microscopy with deep learning allows super-resolution imaging of >1,000 cells in a few hours.
- Wei Ouyang
- , Andrey Aristov
- & Christophe Zimmer
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Resource |
Three-dimensional map of nonhematopoietic bone and bone-marrow cells and molecules
A compendium detailing the localization of bone and bone-marrow-microenvironment cells and proteins will enable efforts to understand the functions of this niche.
- Daniel L Coutu
- , Konstantinos D Kokkaliaris
- & Timm Schroeder
Inferring super-resolution tissue architecture by integrating spatial transcriptomics with histologyBrowse broader subjects
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- Bioluminescence imaging
- 3-D reconstruction
- Diffusion tensor imaging
- Endoscopy
- Fluorescence imaging
- Functional magnetic resonance imaging
- Magnetic resonance imaging
- Magnetoencephalography
- Molecular imaging
- Optical imaging
- Positron-emission tomography
- Time-lapse imaging
- Ultrasound
- Viral tracing
- X-ray tomography
