Existing technologies can map the intricate spatial distribution of biomolecules and cell types within tissues, but not in specimens that remain alive and intact. This study introduces scission-accelerated fluorophore exchange (SAFE) bioorthogonal imaging tools that enable living cells and tissues to be deeply and serially profiled, revealing their biological dynamics across both space and time.
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This is a summary of: Ko, J. et al. Spatiotemporal multiplexed immunofluorescence imaging of living cells and tissues with bioorthogonal cycling of fluorescent probes. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01339-6 (2022).
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Visualization of living cells and tissues in many colors. Nat Biotechnol 40, 1580–1581 (2022). https://doi.org/10.1038/s41587-022-01348-5
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DOI: https://doi.org/10.1038/s41587-022-01348-5
