Featured
-
-
Article
| Open AccessCAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC
Rai et al. report that CAMSAPs can bind to minus ends of microtubules attached to γ-tubulin ring complex (γ-TuRC) and drive microtubule release. They show that CDK5RAP2, but not CLASP2, inhibits CAMSAP-mediated microtubule release from γ-TuRC.
- Dipti Rai
- , Yinlong Song
- & Anna Akhmanova
-
Resource |
Low-input lipidomics reveals lipid metabolism remodelling during early mammalian embryo development
Using low-input lipidomics in mouse and human embryos, Zhang, Shui, Li and colleagues find that lipid unsaturation increases with development towards the blastocyst stage. They further show that lipid desaturases contribute to successful embryo implantation.
- Ling Zhang
- , Jing Zhao
- & Jin Zhang
-
News & Views |
A diffusion barrier limits nuclear leaks
ESCRT-III seals holes in the nuclear envelope (NE). A study now shows that the Cmp7-directed ESCRT-III cascade that grommets and reseals NE holes after spindle pole body (SPB) extrusion at the end of fission yeast mitosis is complemented by the presence of a proteinaceous diffusion barrier to ensure NE integrity.
- Hiral Shah
- & Gautam Dey
-
Article
| Open AccessMyc-dependent dedifferentiation of Gata6+ epidermal cells resembles reversal of terminal differentiation
Bernabé-Rubio et al. report that dedifferentiation of Gata6+ epidermal cells occurs during wound healing or mechanical expansion of the epidermis through a Myc-dependent process that resembles reversal of differentiation.
- Miguel Bernabé-Rubio
- , Shahnawaz Ali
- & Fiona M. Watt
-
Article |
Architecture and dynamics of a desmosome–endoplasmic reticulum complex
Bharathan et al. discover that the endoplasmic reticulum associates with keratin intermediate filaments and desmosomal cell–cell junctions, and that desmosomes and the keratin cytoskeleton regulate the distribution, dynamics and function of the endoplasmic reticulum network.
- Navaneetha Krishnan Bharathan
- , William Giang
- & Andrew P. Kowalczyk
-
Article
| Open AccessPolarized branched Actin modulates cortical mechanics to produce unequal-size daughters during asymmetric division
Daeden et al. report that branched Actin modulates size asymmetry in Drosophila sensory organ precursor cell division.
- Alicia Daeden
- , Alexander Mietke
- & Marcos Gonzalez-Gaitan
-
Article |
Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis
Liu, Nie et al. identify disulfidptosis as a form of cell death resulting from aberrant accumulation of disulfide bonds in actin cytoskeleton proteins that is induced following glucose starvation and dependent on SLC7A11-mediated cystine uptake.
- Xiaoguang Liu
- , Litong Nie
- & Boyi Gan
-
Article
| Open AccessActin-driven chromosome clustering facilitates fast and complete chromosome capture in mammalian oocytes
Harasimov et al. show that, in human and porcine oocytes, actin cables and microtubule loops move chromosomes into a cluster before spindle assembly to ensure fast and complete chromosome capture in meiosis.
- Katarina Harasimov
- , Julia Uraji
- & Melina Schuh
-
News & Views |
+TIPs condense on microtubule plus-ends
End-binding proteins attach to the dynamic plus-ends of microtubules in order to regulate microtubule polymerization and the transport of other plus-end tracking proteins (+TIPs). Three new studies dissect the multivalent interactions that constitute distinct +TIP networks and reveal that they give rise to liquid-like biomolecular condensates.
- Bernardo Gouveia
- & Sabine Petry
-
Article
| Open AccessMultivalency ensures persistence of a +TIP body at specialized microtubule ends
Maan et al., Meier et al. and Song et al. report that microtubule plus-end binding proteins can undergo liquid–liquid phase separation to regulate microtubule dynamics.
- Sandro M. Meier
- , Ana-Maria Farcas
- & Yves Barral
-
Article |
Phase separation of EB1 guides microtubule plus-end dynamics
Maan et al., Meier et al. and Song et al. report that microtubule plus-tip end binding proteins can undergo liquid–liquid phase separation and regulate microtubule dynamics.
- Xiaoyu Song
- , Fengrui Yang
- & Xuebiao Yao
-
Article
| Open AccessMultivalent interactions facilitate motor-dependent protein accumulation at growing microtubule plus-ends
Maan et al., Meier et al. and Song et al. report that microtubule plus-tip end binding proteins can undergo liquid–liquid phase separation and regulate microtubule dynamics.
- Renu Maan
- , Louis Reese
- & Marileen Dogterom
-
News & Views |
A kinesin mimics DNA
Primary cilia transduce cues, including Hedgehog (Hh) signals, and possess doublet microtubules that interact with kinesin motors. The kinesin KIF7 is important for Hh signalling and binds to GLI transcription factors. Haque et al. reveal that, surprisingly, GLI proteins bind a DNA-like part of KIF7 to promote their accumulation at the ciliary tip.
- Dhivya Kumar
- & Jeremy F. Reiter
-
Article
| Open AccessActin remodelling controls proteasome homeostasis upon stress
Williams et al. report that, upon TORC1 inhibition in yeast, mRNA of the chaperone protein ADC17 is localized to cortical actin patches where its translation is enhanced upon stress.
- Thomas David Williams
- , Roberta Cacioppo
- & Adrien Rousseau
-
Article |
Cytoskeletal regulation of a transcription factor by DNA mimicry via coiled-coil interactions
Haque et al. report that the Hedgehog pathway kinesin KIF7 binds the transcription factor GLI via electrostatic coiled-coil interactions, and synergy between KIF7 and GLI underlies the recruitment of both proteins to microtubules and the cilium tip.
- Farah Haque
- , Christian Freniere
- & Radhika Subramanian
-
News & Views |
Centrosomes grow aggresomes to clear waste
Overload of proteasomal clearance triggers formation of a large protein inclusion called the aggresome, which shares similarities with protein aggregates seen in neurodegenerative diseases such as Huntington’s. A new study uncovers how centrosome and centriolar satellite components facilitate stepwise assembly of aggresomes.
- Elisa Vitiello
- & Fanni Gergely
-
Article
| Open AccessAggresome assembly at the centrosome is driven by CP110–CEP97–CEP290 and centriolar satellites
Prosser et al. report that centriolar satellite and centrosomal proteins seed aggresomes, perinuclear inclusions of misfolded proteins, and may play a role in aggresome formation during senescence and huntingtin aggregation.
- Suzanna L. Prosser
- , Johnny Tkach
- & Laurence Pelletier
-
Resource |
Lysate-based pipeline to characterize microtubule-associated proteins uncovers unique microtubule behaviours
Jijumon et al. develop a medium-throughput, lysate-based approach to characterize microtubule interactors, starting here with a set of 45 proteins, and describe unique microtubule behaviours and microtubule-associated activities.
- A. S. Jijumon
- , Satish Bodakuntla
- & Carsten Janke
-
Article |
MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control
By characterizing the composition of mitochondrial-derived vesicles (MDVs), König et al. define a MIRO1/2- and DRP1-dependent MDV biogenesis pathway and propose that MDVs maintain the mitochondrial proteome by shuttling assembled protein complexes to lysosomes.
- Tim König
- , Hendrik Nolte
- & Heidi M. McBride
-
Article |
Forces generated by lamellipodial actin filament elongation regulate the WAVE complex during cell migration
Mehidi et al. show that piconewton forces exerted by the polymerization of individual actin filaments displace the WAVE regulatory complex from lamellipodial tips, thereby regulating WAVE complex activity during cell migration.
- Amine Mehidi
- , Frieda Kage
- & Grégory Giannone
-
Article |
Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration
Pérez-González et al. explore the mechanical properties of intestinal organoids, and report the existence of distinct mechanical domains and that cells are pulled out of the central crypt along a gradient of increasing tension.
- Carlos Pérez-González
- , Gerardo Ceada
- & Xavier Trepat
-
Technical Report |
A reversible shearing DNA probe for visualizing mechanically strong receptors in living cells
Li et al. develop reversible shearing DNA-based tension probes to quantify molecular piconewton-scale forces, estimate the number of mechanically active receptors with single-molecule sensitivity and study mechanisms of force transduction in live cells.
- Hongyun Li
- , Chen Zhang
- & Zheng Liu
-
Article |
Twinfilin uncaps filament barbed ends to promote turnover of lamellipodial actin networks
Hakala et al. report that twinfilin dissociates capping proteins from the actin filament barbed ends to promote actin turnover at leading-edge lamellipodia.
- Markku Hakala
- , Hugo Wioland
- & Pekka Lappalainen
-
News & Views |
Engineered myosins drive filopodial transport
Engineered, light-inducible artificial myosin motors enable selective and direct manipulation of filopodial extensions and provide refined tools to control intracellular cargo transport in vivo.
- Burcu Erdogan
- & Jessica L. Whited
-
Technical Report |
Optogenetic manipulation of cellular communication using engineered myosin motors
Zhang et al. design optogenetically controlled artificial transport vehicles that can be activated reversibly to manipulate cargo transport, impede neurite development and functionally characterize filopodial networks in axolotls.
- Zijian Zhang
- , Nicolas Denans
- & Maria Barna
-
Article |
Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress
Lamm et al. report that replication stress activates mTOR through ATR to induce nuclear actin polymerization, facilitating the recovery from replication stress.
- Noa Lamm
- , Mark N. Read
- & Anthony J. Cesare
-
News & Views |
A MAP for PI3K activation on endosomes
PI3K–Akt signalling downstream of cell-surface receptor activation has long been thought to occur at the plasma membrane. However, surprising evidence now reveals activation of PI3Kα-mediated PI(3,4,5)P3 synthesis on endosomal membranes that is dependent upon the interaction of PI3Kα with the microtubule-associated protein MAP4.
- Alex G. Batrouni
- & Jeremy M. Baskin
-
Article |
Phosphatidylinositol 3-kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4
Intracellularly regulated PI3K activation. Thapa et al. find that phosphatidylinositol-3,4,5-trisphosphate generation and Akt activation occur at intracellular membranes, rather than the plasma membrane, and that this is mediated by MAP4, which controls PI3Kα localization to microtubules.
- Narendra Thapa
- , Mo Chen
- & Richard A. Anderson
-
Article |
ERBB2 drives YAP activation and EMT-like processes during cardiac regeneration
Aharonov et al. use in vivo genetic approaches to show that ErBB2-mediated YAP activation initiates epithelial–mesenchymal transition-like processes and dedifferentiation of cardiomyocytes to drive heart regeneration.
- Alla Aharonov
- , Avraham Shakked
- & Eldad Tzahor
-
Article |
SPIN90 associates with mDia1 and the Arp2/3 complex to regulate cortical actin organization
Cao et al. show that SPIN90 enhances formation of Arp2/3-mediated unbranched filaments and promotes a formin-based cortex by recruiting mDia1 or forming a ternary SPIN90–Arp2/3–mDia1 complex.
- Luyan Cao
- , Amina Yonis
- & Guillaume Charras
-
Article |
Assembly of a persistent apical actin network by the formin Frl/Fmnl tunes epithelial cell deformability
Dehapiot et al. show two actin networks at the cell surface in Drosophila embryos. While the pulsatile network controls local deformation, the Frl-dependent persistent network promotes force propagation.
- Benoit Dehapiot
- , Raphaël Clément
- & Thomas Lecuit
-
Article |
Dynamin regulates the dynamics and mechanical strength of the actin cytoskeleton as a multifilament actin-bundling protein
Zhang et al. show that dynamin forms a helical structure with actin and, upon disruption, enhances branched actin polymerization, constituting a dynamic cycle to regulate actin cytoskeleton mechanical strength.
- Ruihui Zhang
- , Donghoon M. Lee
- & Elizabeth H. Chen
-
News & Views |
LIS1 cracks open dynein
Active transport along microtubules by molecular motors is a crucial cellular process that is disrupted in human diseases. Single-molecule studies from three independent groups reveal a new molecular mechanism for how cells control the activity of the complex microtubule motor cytoplasmic dynein via the neurodevelopmental protein LIS1.
- Richard J. McKenney
-
Article |
Pac1/LIS1 stabilizes an uninhibited conformation of dynein to coordinate its localization and activity
Three independent but complementary studies by the laboratories of Markus, Reck-Peterson and Yildiz identify a key role for LIS1 in modulating localization, activity and function of dynein in cells.
- Matthew G. Marzo
- , Jacqueline M. Griswold
- & Steven M. Markus
-
Letter |
LIS1 promotes the formation of activated cytoplasmic dynein-1 complexes
Three independent but complementary studies by the laboratories of Markus, Reck-Peterson and Yildiz identify a key role for LIS1 in modulating localization, activity and function of dynein in cells.
- Zaw Min Htet
- , John P. Gillies
- & Samara L. Reck-Peterson
-
Article |
Lis1 activates dynein motility by modulating its pairing with dynactin
Lis1 regulates dynein activity. Three independent but complementary studies by the laboratories of Markus, Reck-Peterson and Yildiz identify a key role for Lis1 in modulating localization, activity and function of dynein in cells.
- Mohamed M. Elshenawy
- , Emre Kusakci
- & Ahmet Yildiz
-
Resource |
Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions
Müller et al. provide a comprehensive resource depicting cellular substrates, localization and interacting partners of RhoGEF and RhoGAP proteins regulating the canonical Rho family of GTPases.
- Paul M. Müller
- , Juliane Rademacher
- & Oliver Rocks
-
Article |
A perinuclear microtubule-organizing centre controls nuclear positioning and basement membrane secretion
Zheng et al. discover a type of perinuclear microtubule-organizing center, which is assembled by multiple factors and regulates retrograde endosomal trafficking and plasma membrane growth.
- Yiming Zheng
- , Rebecca A. Buchwalter
- & Timothy L. Megraw
-
Article |
Massive centriole production can occur in the absence of deuterosomes in multiciliated cells
Mercey et al. demonstrate that without deuterosomes, multiciliated cells still develop the proper number of centrioles with normal step-wise kinetics, independent of the growing platform.
- Olivier Mercey
- , Michelle S. Levine
- & Andrew J. Holland
-
Article |
Persistent and polarized global actin flow is essential for directionality during cell migration
Yolland et al. demonstrate persistent flow of the actin flow behind the leading edge and its impact on cell directionality during migration.
- Lawrence Yolland
- , Mubarik Burki
- & Brian M. Stramer
-
Article |
Coupling of β2 integrins to actin by a mechanosensitive molecular clutch drives complement receptor-mediated phagocytosis
Jaumouillé et al. show that a talin/vinculin-based molecular clutch mechanically couples the forces generated by Arp2/3- and Dia1-mediated actin polymerization to promote integrin-mediated phagosome formation.
- Valentin Jaumouillé
- , Alexander X. Cartagena-Rivera
- & Clare M. Waterman
-
Article |
The nucleoskeleton protein IFFO1 immobilizes broken DNA and suppresses chromosome translocation during tumorigenesis
Li et al. find that IFFO1 bridges the core NHEJ factor XRCC4 and lamin A/C, thus reducing the mobility of broken DNA ends to prevent chromosomal translocation in cancer cells.
- Wen Li
- , Xiuzhen Bai
- & Dongyi Xu
-
Review Article |
Nuclear actin filaments in DNA repair dynamics
In this Review, Caridi et al. discuss actin filaments in the nucleus and the functions of nuclear F-actin in response to DNA double-strand break repair.
- Christopher Patrick Caridi
- , Matthias Plessner
- & Irene Chiolo
-
Article |
The inner centromere is a biomolecular condensate scaffolded by the chromosomal passenger complex
Trivedi et al. find that phase separation of the chromosome passenger complex is essential for its localization and function at the inner centromere during mitosis.
- Prasad Trivedi
- , Francesco Palomba
- & P. Todd Stukenberg
-
News & Views |
KIFC3 directs a centrosome cohesive force
Assembly of the mitotic spindle requires timely separation of the centrosomes. Their movement apart is driven by the plus-end-directed kinesin Eg5. A new study demonstrates that the kinesin KIFC3 provides an opposing microtubule-based cohesive force that modulates centrosome separation and ensures accurate chromosome segregation.
- Ciaran G. Morrison
-
Article |
The balance between KIFC3 and EG5 tetrameric kinesins controls the onset of mitotic spindle assembly
Hata et al. report that minus-end-directed kinesin KIFC3 promotes centrosome cohesion at the onset of mitosis, counteracting centrosome separation driven by the plus-end-directed kinesin EG5.
- Shoji Hata
- , Ana Pastor Peidro
- & Elmar Schiebel
-
Letter |
Kinetically distinct phases of tau on microtubules regulate kinesin motors and severing enzymes
Tan et al. and Siahaan et al. present distinct but complementary data showing that microtubule regulates dynamic condensation of tau molecules, and this in turn affects microtubule biology and function.
- Valerie Siahaan
- , Jochen Krattenmacher
- & Marcus Braun
-
Letter |
Microtubules gate tau condensation to spatially regulate microtubule functions
Tan et al. and Siahaan et al. present distinct but complementary data showing that microtubule regulates dynamic condensation of tau molecules, and this in turn affects microtubule biology and function.
- Ruensern Tan
- , Aileen J. Lam
- & Richard J. McKenney
-
Letter |
Dynamic polyhedral actomyosin lattices remodel micron-scale curved membranes during exocytosis in live mice
Using intravital imaging, Ebrahim et al. show that actin and non-muscle myosin II assemble into polyhedral lattices around the vesicle membrane to mediate exocytic secretion in live tissues.
- Seham Ebrahim
- , Desu Chen
- & Roberto Weigert