Protein Dimerization on a Phosphonated Calix[6]arene Disc

Journal:
Angewandte Chemie International Edition
Published:
DOI:
10.1002/anie.201701500
Affiliations:
2
Authors:
4

Research Highlight

Connecting proteins with calixarene molecular glue

©aalexx/Getty

Calixarenes are large molecules with atoms arranged in a ring with a puckered structure.  A team from Flinders University in Australia and colleagues has shown that the unusual shape of calixarenes can mimic protein binding sites and fix elusive biomolecules into well-defined crystal lattices.

The researchers deduced that calixarene discs loaded with phosphorous-oxygen ions attach to a single binding site on the protein cytochrome c. They then stack two proteins back-to-back to form a ‘dimer’ complex. These small nanostructures drive crystallization of an ordered complex that locks the proteins into place, and keeps them at distances from each other — an ideal situation for x-ray-based structure analysis of this and possibly other biomolecules.

Supported content

References

  1. Angewandte Chemie International Edition 56, 5517–5521 (2017). doi: 10.1002/anie.201701500
Institutions Authors Share
National University of Ireland Galway (NUI Galway), Ireland
3.000000
0.75
Flinders University, Australia
1.000000
0.25