Biological techniques

Definition

Biological techniques are methods or procedures that are used to study living things. They include experimental and computational methods, approaches, protocols and tools for biological research.

Featured

Latest Research and Reviews

  • Research | | open

    Screening libraries of genetically engineered microbes for secreted products is limited by the available assay throughput. Here the authors combine aptamer-based fluorescent detection with droplet microfluidics to achieve high throughput screening of yeast strains engineered for enhanced tyrosine or streptavidin production.

    • Joseph Abatemarco
    • , Maen F. Sarhan
    • , James M. Wagner
    • , Jyun-Liang Lin
    • , Leqian Liu
    • , Wafa Hassouneh
    • , Shuo-Fu Yuan
    • , Hal S. Alper
    •  & Adam R. Abate
  • Research |

    Crystal structures of the HIV-1 Env trimer with cell-entry inhibitor antiviral drug leads BMS-378806 and BMS-626529 along with biophysical data define allosteric and competitive mechanisms to inhibit CD4-induced structural changes in Env.

    • Marie Pancera
    • , Yen-Ting Lai
    • , Tatsiana Bylund
    • , Aliaksandr Druz
    • , Sandeep Narpala
    • , Sijy O'Dell
    • , Arne Schön
    • , Robert T Bailer
    • , Gwo-Yu Chuang
    • , Hui Geng
    • , Mark K Louder
    • , Reda Rawi
    • , Djade I Soumana
    • , Andrés Finzi
    • , Alon Herschhorn
    • , Navid Madani
    • , Joseph Sodroski
    • , Ernesto Freire
    • , David R Langley
    • , John R Mascola
    • , Adrian B McDermott
    •  & Peter D Kwong
  • Research |

    An atlas of microRNA expression patterns and regulators is produced by deep sequencing of short RNAs in human and mouse cells.

    • Derek de Rie
    • , Imad Abugessaisa
    • , Tanvir Alam
    • , Erik Arner
    • , Peter Arner
    • , Haitham Ashoor
    • , Gaby Åström
    • , Magda Babina
    • , Nicolas Bertin
    • , A Maxwell Burroughs
    • , Ailsa J Carlisle
    • , Carsten O Daub
    • , Michael Detmar
    • , Ruslan Deviatiiarov
    • , Alexandre Fort
    • , Claudia Gebhard
    • , Daniel Goldowitz
    • , Sven Guhl
    • , Thomas J Ha
    • , Jayson Harshbarger
    • , Akira Hasegawa
    • , Kosuke Hashimoto
    • , Meenhard Herlyn
    • , Peter Heutink
    • , Kelly J Hitchens
    • , Chung Chau Hon
    • , Edward Huang
    • , Yuri Ishizu
    • , Chieko Kai
    • , Takeya Kasukawa
    • , Peter Klinken
    • , Timo Lassmann
    • , Charles-Henri Lecellier
    • , Weonju Lee
    • , Marina Lizio
    • , Vsevolod Makeev
    • , Anthony Mathelier
    • , Yulia A Medvedeva
    • , Niklas Mejhert
    • , Christopher J Mungall
    • , Shohei Noma
    • , Mitsuhiro Ohshima
    • , Mariko Okada-Hatakeyama
    • , Helena Persson
    • , Patrizia Rizzu
    • , Filip Roudnicky
    • , Pål Sætrom
    • , Hiroki Sato
    • , Jessica Severin
    • , Jay W Shin
    • , Rolf K Swoboda
    • , Hiroshi Tarui
    • , Hiroo Toyoda
    • , Kristoffer Vitting-Seerup
    • , Louise Winteringham
    • , Yoko Yamaguchi
    • , Kayoko Yasuzawa
    • , Misako Yoneda
    • , Noriko Yumoto
    • , Susan Zabierowski
    • , Peter G Zhang
    • , Christine A Wells
    • , Kim M Summers
    • , Hideya Kawaji
    • , Albin Sandelin
    • , Michael Rehli
    • , The FANTOM Consortium
    • , Yoshihide Hayashizaki
    • , Piero Carninci
    • , Alistair R R Forrest
    •  & Michiel J L de Hoon
  • Research |

    The statistical concepts for false discovery rate control long applied in the field of data-dependent acquisition (DDA) mass spectrometry-based proteomics can be adapted for the emerging technique of data-independent acquisition (DIA) mass spectrometry.

    • George Rosenberger
    • , Isabell Bludau
    • , Uwe Schmitt
    • , Moritz Heusel
    • , Christie L Hunter
    • , Yansheng Liu
    • , Michael J MacCoss
    • , Brendan X MacLean
    • , Alexey I Nesvizhskii
    • , Patrick G A Pedrioli
    • , Lukas Reiter
    • , Hannes L Röst
    • , Stephen Tate
    • , Ying S Ting
    • , Ben C Collins
    •  & Ruedi Aebersold
  • Research |

    Horton et al. show that early Crebbp loss in haematopoietic progenitors results in a defective p53-mediated DNA damage response, leading to the accumulation of epigenetic and genetic alterations, which promote the onset of lymphoid malignancies.

    • Sarah J. Horton
    • , George Giotopoulos
    • , Haiyang Yun
    • , Shabana Vohra
    • , Olivia Sheppard
    • , Rachael Bashford-Rogers
    • , Mamunur Rashid
    • , Alexandra Clipson
    • , Wai-In Chan
    • , Daniel Sasca
    • , Loukia Yiangou
    • , Hikari Osaki
    • , Faisal Basheer
    • , Paolo Gallipoli
    • , Natalie Burrows
    • , Ayşegül Erdem
    • , Anastasiya Sybirna
    • , Sarah Foerster
    • , Wanfeng Zhao
    • , Tonci Sustic
    • , Anna Petrunkina Harrison
    • , Elisa Laurenti
    • , Jessica Okosun
    • , Daniel Hodson
    • , Penny Wright
    • , Ken G. Smith
    • , Patrick Maxwell
    • , Jude Fitzgibbon
    • , Ming Q. Du
    • , David J. Adams
    •  & Brian J. P. Huntly
  • Research |

    Monocle 2 uses reversed graph embedding to automatically learn complex, branched pseudotime trajectories of differentiation or cellular state changes from single-cell expression data.

    Monocle 2 uses reversed graph embedding to automatically learn complex, branched pseudotime trajectories of differentiation or cellular state changes from single-cell expression data.

    • Xiaojie Qiu
    • , Qi Mao
    • , Ying Tang
    • , Li Wang
    • , Raghav Chawla
    • , Hannah A Pliner
    •  & Cole Trapnell

News and Comment