Identification of host-induced pathogen genes by differential fluorescence induction reporter systems - pp770 - 777

Dirk Bumann & Raphael H Valdivia

Published online: 05 April 2007 | doi:10.1038/nprot.2007.78

Abstract - Identification of host-induced pathogen genes by differential fluorescence induction reporter systems | Full Text - Identification of host-induced pathogen genes by differential fluorescence induction reporter systems | PDF (561 KB) - Identification of host-induced pathogen genes by differential fluorescence induction reporter systems

Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry - pp778 - 791

Ric CH De Vos, Sofia Moco, Arjen Lommen, Joost JB Keurentjes, Raoul J Bino & Robert D Hall

Published online: 05 April 2007 | doi:10.1038/nprot.2007.95

Abstract - Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry | Full Text - Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry | PDF (738 KB) - Untargeted large-scale plant metabolomics using liquid chromatography coupled to mass spectrometry

A method for genetic modification of human embryonic stem cells using electroporation - pp792 - 796

Magdaline Costa, Mirella Dottori, Koula Sourris, Pegah Jamshidi, Tanya Hatzistavrou, Richard Davis, Lisa Azzola, Steven Jackson, Sue Mei Lim, Martin Pera, Andrew G Elefanty & Edouard G Stanley

Published online: 05 April 2007 | doi:10.1038/nprot.2007.105

Abstract - A method for genetic modification of human embryonic stem cells using electroporation | Full Text - A method for genetic modification of human embryonic stem cells using electroporation | PDF (428 KB) - A method for genetic modification of human embryonic stem cells using electroporation

In vivo imaging of subcutaneous structures using functional photoacoustic microscopy - pp797 - 804

Hao F Zhang, Konstantin Maslov & Lihong V Wang

Published online: 05 April 2007 | doi:10.1038/nprot.2007.108

Abstract - In vivo: imaging of subcutaneous structures using functional photoacoustic microscopy | Full Text - In vivo imaging of subcutaneous structures using functional photoacoustic microscopy | PDF (346 KB) - In vivo imaging of subcutaneous structures using functional photoacoustic microscopy

A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood - pp805 - 810

Dan G Duda, Kenneth S Cohen, David T Scadden & Rakesh K Jain

Published online: 05 April 2007 | doi:10.1038/nprot.2007.111

Abstract - A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood | Full Text - A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood | PDF (259 KB) - A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood

Genetic manipulation of Aspergillus nidulans: meiotic progeny for genetic analysis and strain construction - pp811 - 821

Richard B Todd, Meryl A Davis & Michael J Hynes

Published online: 05 April 2007 | doi:10.1038/nprot.2007.112

Abstract - Genetic manipulation of : Aspergillus nidulans: : meiotic progeny for genetic analysis and strain construction | Full Text - Genetic manipulation of Aspergillus nidulans: meiotic progeny for genetic analysis and strain construction | PDF (479 KB) - Genetic manipulation of Aspergillus nidulans: meiotic progeny for genetic analysis and strain construction

See also: Protocol by Todd et al.

Genetic manipulation of Aspergillus nidulans: heterokaryons and diploids for dominance, complementation and haploidization analyses - pp822 - 830

Richard B Todd, Meryl A Davis & Michael J Hynes

Published online: 05 April 2007 | doi:10.1038/nprot.2007.113

Abstract - Genetic manipulation of : Aspergillus nidulans: : heterokaryons and diploids for dominance, complementation and haploidization analyses | Full Text - Genetic manipulation of Aspergillus nidulans: heterokaryons and diploids for dominance, complementation and haploidization analyses | PDF (413 KB) - Genetic manipulation of Aspergillus nidulans: heterokaryons and diploids for dominance, complementation and haploidization analyses

See also: Protocol by Todd et al.

Protocol for the purification of proteins from biological extracts for identification by mass spectrometry - pp831 - 837

Luc Guerrier & Egisto Boschetti

Published online: 05 April 2007 | doi:10.1038/nprot.2007.114

Abstract - Protocol for the purification of proteins from biological extracts for identification by mass spectrometry | Full Text - Protocol for the purification of proteins from biological extracts for identification by mass spectrometry | PDF (271 KB) - Protocol for the purification of proteins from biological extracts for identification by mass spectrometry

RNA stable-isotope probing - pp838 - 844

Andrew S Whiteley, Bruce Thomson, Tillmann Lueders & Mike Manefield

Published online: 12 April 2007 | doi:10.1038/nprot.2007.115

Abstract - RNA stable-isotope probing | Full Text - RNA stable-isotope probing | PDF (338 KB) - RNA stable-isotope probing

See also: Protocol by Neufeld et al.

Practical and convenient synthesis of coumarins from phenols and propiolic acid esters - pp845 - 848

Tsugio Kitamura, Juzo Oyamada & Toshiki Tsubota

Published online: 05 April 2007 | doi:10.1038/nprot.2007.103

Abstract - Practical and convenient synthesis of coumarins from phenols and propiolic acid esters | Full Text - Practical and convenient synthesis of coumarins from phenols and propiolic acid esters | PDF (135 KB) - Practical and convenient synthesis of coumarins from phenols and propiolic acid esters

A combined ex/in vivo assay to detect effects of exogenously added factors in neural stem cells - pp849 - 859

Sacri R Ferrón, Celia Andreu-Agulló, Helena Mira, Pilar Sánchez, M Ángeles Marqués-Torrejón & Isabel Fariñas

Published online: 05 April 2007 | doi:10.1038/nprot.2007.104

Abstract - A combined : ex/in vivo: assay to detect effects of exogenously added factors in neural stem cells | Full Text - A combined ex/in vivo assay to detect effects of exogenously added factors in neural stem cells | PDF (387 KB) - A combined ex/in vivo assay to detect effects of exogenously added factors in neural stem cells

DNA stable-isotope probing - pp860 - 866

Josh D Neufeld, Jyotsna Vohra, Marc G Dumont, Tillmann Lueders, Mike Manefield, Michael W Friedrich & J Colin Murrell

Published online: 12 April 2007 | doi:10.1038/nprot.2007.109

Abstract - DNA stable-isotope probing | Full Text - DNA stable-isotope probing | PDF (298 KB) - DNA stable-isotope probing

See also: Protocol by Whiteley et al.

Rapid measurement of total antioxidant capacity in plants - pp867 - 870

Kelly M Gillespie, June M Chae & Elizabeth A Ainsworth

Published online: 12 April 2007 | doi:10.1038/nprot.2007.100

Abstract - Rapid measurement of total antioxidant capacity in plants | Full Text - Rapid measurement of total antioxidant capacity in plants | PDF (206 KB) - Rapid measurement of total antioxidant capacity in plants

See also: Protocol by Gillespie & Ainsworth | Protocol by Ainsworth & Gillespie

Measurement of reduced, oxidized and total ascorbate content in plants - pp871 - 874

Kelly M Gillespie & Elizabeth A Ainsworth

Published online: 12 April 2007 | doi:10.1038/nprot.2007.101

Abstract - Measurement of reduced, oxidized and total ascorbate content in plants | Full Text - Measurement of reduced, oxidized and total ascorbate content in plants | PDF (128 KB) - Measurement of reduced, oxidized and total ascorbate content in plants

See also: Protocol by Gillespie et al. | Protocol by Ainsworth & Gillespie

Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent - pp875 - 877

Elizabeth A Ainsworth & Kelly M Gillespie

Published online: 12 April 2007 | doi:10.1038/nprot.2007.102

Abstract - Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent | Full Text - Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent | PDF (114 KB) - Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent

See also: Protocol by Gillespie et al. | Protocol by Gillespie & Ainsworth

Total internal reflection with fluorescence correlation spectroscopy - pp878 - 890

Nancy L Thompson & Bridgett L Steele

Published online: 12 April 2007 | doi:10.1038/nprot.2007.110

Abstract - Total internal reflection with fluorescence correlation spectroscopy | Full Text - Total internal reflection with fluorescence correlation spectroscopy | PDF (309 KB) - Total internal reflection with fluorescence correlation spectroscopy

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes - pp891 - 903

Manfred T Reetz & José Daniel Carballeira

Published online: 12 April 2007 | doi:10.1038/nprot.2007.72

Abstract - Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes | Full Text - Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes | PDF (718 KB) - Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances - pp904 - 923

Olav Schiemann, Nelly Piton, Jörn Plackmeyer, Bela E Bode, Thomas F Prisner & Joachim W Engels

Published online: 12 April 2007 | doi:10.1038/nprot.2007.97

Abstract - Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances | Full Text - Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances | PDF (622 KB) - Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances

Gene splicing and mutagenesis by PCR-driven overlap extension - pp924 - 932

Karin L Heckman & Larry R Pease

Published online: 12 April 2007 | doi:10.1038/nprot.2007.132

Abstract - Gene splicing and mutagenesis by PCR-driven overlap extension | Full Text - Gene splicing and mutagenesis by PCR-driven overlap extension | PDF (323 KB) - Gene splicing and mutagenesis by PCR-driven overlap extension

Chemical derivatization of histones for facilitated analysis by mass spectrometry - pp933 - 938

Benjamin A Garcia, Sahana Mollah, Beatrix M Ueberheide, Scott A Busby, Tara L Muratore, Jeffrey Shabanowitz & Donald F Hunt

Published online: 12 April 2007 | doi:10.1038/nprot.2007.106

Abstract - Chemical derivatization of histones for facilitated analysis by mass spectrometry | Full Text - Chemical derivatization of histones for facilitated analysis by mass spectrometry | PDF (192 KB) - Chemical derivatization of histones for facilitated analysis by mass spectrometry

Preparation of cultured myofibers from larval salamander limbs for cellular plasticity studies - pp939 - 947

Anoop Kumar & Jeremy P Brockes

Published online: 19 April 2007 | doi:10.1038/nprot.2007.134

Abstract - Preparation of cultured myofibers from larval salamander limbs for cellular plasticity studies | Full Text - Preparation of cultured myofibers from larval salamander limbs for cellular plasticity studies | PDF (457 KB) - Preparation of cultured myofibers from larval salamander limbs for cellular plasticity studies

Agrobacterium rhizogenes-mediated transformation of soybean to study root biology - pp948 - 952

Attila Kereszt, Dongxue Li, Arief Indrasumunar, Cuc DT Nguyen, Sureeporn Nontachaiyapoom, Mark Kinkema & Peter M Gresshoff

Published online: 19 April 2007 | doi:10.1038/nprot.2007.141

Abstract - Agrobacterium rhizogenes: -mediated transformation of soybean to study root biology | Full Text - Agrobacterium rhizogenes-mediated transformation of soybean to study root biology | PDF (259 KB) - Agrobacterium rhizogenes-mediated transformation of soybean to study root biology | Supplementary information

Locating proteins in the cell using TargetP, SignalP and related tools - pp953 - 971

Olof Emanuelsson, Søren Brunak, Gunnar von Heijne & Henrik Nielsen

Published online: 19 April 2007 | doi:10.1038/nprot.2007.131

Abstract - Locating proteins in the cell using TargetP, SignalP and related tools | Full Text - Locating proteins in the cell using TargetP, SignalP and related tools | PDF (799 KB) - Locating proteins in the cell using TargetP, SignalP and related tools

An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with ^99mTc at room temperature - pp972 - 978

Yi Wang, Xinrong Liu & Donald J Hnatowich

Published online: 19 April 2007 | doi:10.1038/nprot.2007.144

Abstract - An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with : 99m: Tc at room temperature | Full Text - An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with 99mTc at room temperature | PDF (208 KB) - An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with 99mTc at room temperature

A protocol for conducting 7-day daily renewal tests with Lemna gibba - pp979 - 987

Richard A Brain & Keith R Solomon

Published online: 19 April 2007 | doi:10.1038/nprot.2007.146

Abstract - A protocol for conducting 7-day daily renewal tests with : Lemna gibba | Full Text - A protocol for conducting 7-day daily renewal tests with Lemna gibba | PDF (505 KB) - A protocol for conducting 7-day daily renewal tests with Lemna gibba

Mapping networks of physical interactions between genomic elements using 5C technology - pp988 - 1002

Josée Dostie & Job Dekker

Published online: 19 April 2007 | doi:10.1038/nprot.2007.116

Abstract - Mapping networks of physical interactions between genomic elements using 5C technology | Full Text - Mapping networks of physical interactions between genomic elements using 5C technology | PDF (499 KB) - Mapping networks of physical interactions between genomic elements using 5C technology

Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studies - pp1003 - 1012

Kevin Strange, Michael Christensen & Rebecca Morrison

Published online: 19 April 2007 | doi:10.1038/nprot.2007.143

Abstract - Primary culture of : Caenorhabditis elegans: developing embryo cells for electrophysiological, cell biological and molecular studies | Full Text - Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studies | PDF (473 KB) - Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological and molecular studies

Synthesis, purification and sample experiment for fluorescent pteridine-containing DNA: tools for studying DNA interactive systems - pp1013 - 1021

Mary E Hawkins

Published online: 19 April 2007 | doi:10.1038/nprot.2007.150

Abstract - Synthesis, purification and sample experiment for fluorescent pteridine-containing DNA: tools for studying DNA interactive systems | Full Text - Synthesis, purification and sample experiment for fluorescent pteridine-containing DNA: tools for studying DNA interactive systems | PDF (275 KB) - Synthesis, purification and sample experiment for fluorescent pteridine-containing DNA: tools for studying DNA interactive systems