Welcome to Nature Protocols
Nature Protocols is an online resource for protocols, including authoritative, peer-reviewed 'Nature Protocols' and an interactive 'Protocols Network'. The two create a dynamic forum for scientists to upload and comment on protocols.
Featured Protocols
COMPUTATIONAL BIOLOGY
Visualizing 3D nucleic acid structures with 3DNAThis article contains protocols for 5 real-world applications that give a clear introduction to 3DNA - a software system that can be used to analyze, rebuild and visualize 3D nucleic-acid-containing structures, including their complexes with other molecules.
NUCLEIC ACID BASED MOLECULAR BIOLOGY
Improved northern blot method for enhanced detection of small RNAThis northern blotting protocol uses chemical crosslinking rather than UV-crosslinking to enhance the detection of small RNAs (< 40 nucleotides), including regulatory species such as miRNA, siRNA and piRNA.
BIOCHEMISTRY AND PROTEIN ANALYSIS
Quantitative DNase footprint titration: a tool for analyzing the energetics of protein-DNA interactionsDNase footprinting is often used to determine the site of interaction between proteins and DNA. However, information about the energetics of a protein-DNA interaction can be obtained by carrying out a DNase footprint titration with a range of protein concentrations, as described in this protocol.
BIOCHEMISTRY AND PROTEIN ANALYSIS
Detection of low-abundance proteins in biological extractsIn this elegant method, the protein extract is mixed with a library of hexapeptide-ligand coated beads. Theoretically, the maximum amount of each protein that can bind is the same. After removal of the unbound components, those proteins present in excess of the ligand concentration are diluted, while those of relatively lower abundance are concentrated.
NUCLEIC ACID BASED MOLECULAR BIOLOGY
Loop-mediated isothermal amplification (LAMP) of gene sequencesThis protocol describes the loop-mediated isothermal amplification (LAMP) method of gene amplification. It also uses a fluorescent metal indicator system to allow simple visual detection of products.
Protocols Network
There are various ways you can contribute:
Upload your own lab protocols for further feedback and refinement
Discuss techniques in our discussion forum
Highlights from the network
- Detection of Heteromerization of More than Two Proteins by Sequential BRET-FRET
- Multiple-Target Tracing (MTT) Algorithm Probes Molecular Dynamics at Cell Surface
- Neural Stem Cell Culture (1 comment)
- A method for ameliorating autoimmune disease by passive transfer of IVIg-primed leukocytes (1 comment)
- Monitoring Solid Phase Peptide Synthesis
- Direct thionation of reducing sugars
- Analysis protein complexes by 1D-SDS-PAGE and tandem mass spectrometry
- Analysis protein complexes by 1D-SDS-PAGE and tandem mass spectrometry
- Deathless transgenesis: A new spermatogonia mediated in vivo approach for generation of transgenic mice
- Modeling evolution of insect resistance to genetically modified crops
Noticeboard
Browse categories
- Biochemistry and protein analysis
- Cell and developmental biology
- Cell and tissue culture
- Chemical modification
- Computational and theoretical biology
- Genetic analysis
- Genetic modification
- Genomics and proteomics
- Imaging
- Immunological techniques
- Isolation, purification and separation
- Microbiology and virology
- Model organisms
- Nanotechnology
- Neuroscience
- Nucleic acid based molecular biology
- Pharmacology and toxicology
- Plant biology
- Spectroscopy and structural analysis
- Synthetic chemistry
Comment on published Nature Protocols, or protocols submitted by other researchers