Mass spectrum resulting from the Glyco- copper-catalysed Huisgen cycloadditon reaction of SSβG-Aha43 (a β-Galactosidase mutant protein from Sulfolobus solfataricus with single azide tag) with GlcNAc-O-CH2-alkyne. This is an example of a site-selective protein glycosylation. Image is from the protocol by van Kasteren et al. Cover by Jessica Iannuzzi.

DNA silver staining of PAGE separated arbitrary primed DNA amplification products. Image is from the protocol by Basso and Gresshoff. Cover image by Jessica Iannuzzi.

Toponome colocalization map of a CD4 T-lymphocyte with clear-cut cell surface protein clusters singled out in different colours. Image is from the protocol by Schubert et al. Cover by Jessica Iannuzzi.

Scanning electron micrograph of nuclear pore complexes (yellow) in the nuclear envelope of S. cerevisiae. Image is from the protocol by Kiseleva et al. Cover by Jessica Iannuzzi.

Equipment set-up for addition of the lithium in the ammonia-free Birch Reduction. Strips of lithium are removed from pentane using tweezers and cut into small portions with scissors while holding them above the mouth of the neck of the flask (directly underneath a funnel attached to an argon line), allowing them to fall into the reaction vessel as they are cut. Photograph by Karl Harrison and published in the protocol by Donohoe and Thomas.

Relative orientation and dynamics of A-form RNA helices using an order tensor analysis of Residual Dipolar Couplings. This protocol is particularly valuable for exploring adaptive changes in RNA conformation that occur in response to biologically relevant signals

Velocity 11 VPrep Automated Liquid Handling System dispensing samples in a 384-well format. In this image Coomassie blue is being distributed into wells to ensure reproducible and accurate liquid handling in the range of 1-30 microlitres. Photo taken by Caleb Foster, one of the authors of the protocol by Tierno et al., describing the development and optimization of high-throughput in vitro protein phosphatase screening assays. Cover by Jessica Iannuzzi.

Fluorescent micrograph of cultured Caenorhabditis elegans mechanosensory neurons expressing mec-4::GFP. Cell nuclei are labelled with DAPI and shown in blue. Image is from the protocol by Strange et al., which describes the large-scale primary culture of C. elegans developing embryo cells for use in electrophysiological, cell biological and molecular studies. This protocol also details methods for inducing RNA interference (RNAi) in embryo cell cultures, isolating specific cell types from cultures by fluorescence activated cell sorting (FACS), and patch clamp electrophysiology of cultured cells. Cover by Jessica Iannuzzi.

Colonies of the ascomycete Aspergillus nidulans growing on agar medium. Image from the protocol by Hynes et al. Cover by Jessica Iannuzzi.

Overlaid crystal structures of hepatitis B virus with (red, PDB file 2G34) and without (blue, 2G33) a small molecule that enhances assembly and distorts capsid structure: in the presence of HAP1, fivefolds are at greater radius and quasi-sixfolds are flattened. Image courtesy of Cristina Bourne, one of the authors of the protocol by Zlotnick et al.

Two successive steps in mouse piezo intracytoplasmic sperm injection. From the protocol by Naoko Yoshida and Anthony Perry describing piezo-actuated mouse intracytoplasmic sperm injection. Cover by Jessica Iannuzzi.

Low-power view of a dorsal-root ganglion neuron in a thyl-GFP-S mouse. From the protocol by Misgeld et al. describing in vivo imaging of single axons in the mouse spinal cord. Cover by Jessica Iannuzzi.