This Perspective reviews efforts to map six different RNA modifications — pseudouridine (Ψ), 5-methylcytidine (m5C), N
1-methyladenosine (m1A), N
4-acetylcytidine (ac4C), ribose methylations (Nm) and N
7-methylguanosine (m7G) — and how they differ from N
6-methyladenosine (m6A). The authors discuss the technical and analytical challenges of characterizing the epitranscriptome and provide their own conclusions on the abundance and distribution of these modifications.
- David Wiener
- Schraga Schwartz