Abstract
Apoptosis resistance is a hurdle for cancer treatment. HECTD3, a new E3 ubiquitin ligase, interacts with caspase-8 death effector domains and ubiquitinates caspase-8 with K63-linked polyubiquitin chains that do not target caspase-8 for degradation but decrease the caspase-8 activation. HECTD3 depletion can sensitize cancer cells to extrinsic apoptotic stimuli. In addition, HECTD3 inhibits TNF-related apoptosis-inducing ligand (TRAIL)-induced caspase-8 cleavage in an E3 ligase activity-dependent manner. Mutation of the caspase-8 ubiquitination site at K215 abolishes the HECTD3 protection from TRAIL-induced cleavage. Finally, HECTD3 is frequently overexpressed in breast carcinomas. These findings suggest that caspase-8 ubiquitination by HECTD3 confers cancer cell survival.
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Main
Apoptosis is a critical cellular process required for the maintenance of tissue homeostasis; dysregulation of apoptosis is a major contributor to human pathologies including cancer. Caspases, a family of cysteine proteases, are common death effector molecules that cleave a number of different substrates.1 Caspase activation can be initiated by the mitochondrial (intrinsic) or death receptor (extrinsic) pathways.2 The extrinsic pathway is activated by TNF family ligands, including TNFα, FasL, and the TNF-related apoptosis-inducing ligand (TRAIL) through the respective binding with their specific receptors. Subsequently, an initial caspase, pro-caspase-8, is recruited to the oligomeric membrane-associated death-inducing signaling complex (DISC) through FADD, resulting in pro-caspase-8 oligomerization and self-cleavage.3, 4 Activated caspase-8 induces apoptosis by directly cleaving effector caspases, e.g., caspase-3 and -7.5 The available evidence suggests that caspase activation is regulated by ubiquitination. Several inhibitors of apoptosis (IAP) E3 ligases, XIAP and cIAP1/2, inhibit caspase-9, -7, and -3 activation through direct interaction6, 7, 8 and ubiquitination.9, 10 Caspase-8 ubiquitination at its C-terminus by a CUL3-based E3 ligase complex promotes caspase-8 activation and apoptosis.11 Peng et al. reported that EGF induces caspase-8 phosphorylation, ubiquitination, and degradation, although the responsible E3 ligase is unknown.12 E3 ubiquitin ligases SIAH2 and POSH have been shown to inhibit caspase-8 activity;13 however, whether these E3 ligases ubiquitinates caspase-8 has not been tested. TRAF2-mediated K48-linked polyubiquitination on the large catalytic domain (p18) of caspase-8 increases the degradation of active caspase-8 and the signal threshold for death receptor-mediated apoptosis.14 Consistently, inhibition of the proteasomal degradation of p18 sensitizes cancer cells to TRAIL-induced apoptosis.15, 16
Ubiquitination regulates multiple cellular processes including apoptosis. The ubiquitin (Ub) can be conjugated to the substrate’s lysine (K) residues through isopeptide bonds. Protein ubiquitination is sequentially mediated by three enzymes: the ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3) that controls substrate specificity. Ub is conjugated either as a single moiety or as polyubiquitin chains linked through K48, K63, or other K residues of Ub with different functional consequences. K48-linked polyubiquitin chains target substrates to the 26S proteasome for degradation while K63-linked polyubiquitin chains initiate non-degradation signaling.17
E3 ligases partition into two subfamilies; the RING finger domain-containing E3s and the HECT (homologous to E6-AP COOH terminus) domain-containing E3s.18, 19 All 28 HECT-type E3s contain a conserved C-terminal HECT domain and a highly variable N-terminal domain that is responsible for substrate binding.20, 21, 22, 23, 24 The HECT domain-containing 3 (HECTD3) E3 ligase contains an N-terminal DOC (destruction of cyclin) domain. The DOC domain has been linked to substrate recognition in several E3 ligases including the anaphase-promoting complex subunit 10 (APC10/DOC1),25 PARC, CUL7, and HERC2.26 N-terminal-truncated HECTD3 targets Tara (Trio-associated repeat on actin) for ubiquitin-mediated degradation.27 In addition, HECTD3 depletion induces multipolar spindle formation in HeLa cells.27 Moreover, HECTD3 has been shown to ubiquitinate Syntaxin-8.28 Most recently, we reported that HECTD3 ubiquitinates MALT1 with nondegradative polyubiquitin chains, stabilizes MALT1, and confers cancer cells to cisplatin.29 The role and action mechanism of HECTD3 in cancer, however, is not completely understood.
Results
HECTD3 interacts with caspase-8 through the DOC/DED domains
HECTD3 ubiquitin E3 ligase interacts with MALT1,29 which has been reported to form complex with Caspase-8.30 We wondered whether HECTD3 interacts with caspase-8. The protein interaction between HECTD3 and caspase-8 was confirmed by co-immunoprecipitation (IP). HECTD3 specifically interacted with the endogenous caspase-8 but not caspase-3 and -7 compared with HECTD3Δ1-511, which does not have the DOC domain (Figures 1a and b). The HECTD3-caspase-8 protein interaction was further confirmed by a reciprocal co-immunoprecipitation experiment (Figure 1c). The GST pull-down experiment indicated that the purified recombinant HECTD3 protein from E.coli (Supplementary Figure S1A) interacted with the caspase-8 protein translated in vitro using a cell-free in vitro translation system (Figure 1d). In contrast, HECTD3 failed to pull-down the in vitro translated caspase-3 protein (Figure 1d). These results indicated that HECTD3 specifically and directly interacts with caspase-8. We further demonstrated that the endogenous HECTD3 protein interacted with the endogenous caspase-8 proteins in HeLa (Figure 1e). These results suggest that HECTD3 and caspase-8 interact with each other at the physiological level. The localization of Flag-HECTD3 and caspsase-8 in HEK293T cells were evaluated by immunofluorescence staining. As shown in Figure 1f, both Flag-HECTD3 and caspsase-8 are predominately localized in the cytoplasm.
The HECTD3 protein domain responsible for caspase-8 binding was mapped. As shown in Figure 1g, depletion of the N-terminal 511 residues from HECTD3 resulted in the loss of binding with caspase-8. The smallest HECTD3 domain responsible for caspase-8 binding was mapped to a region between 109 and 393 that harbors the DOC domain (Figure 1a). Compared with the wild-type (WT) HECTD3, several HECTD3 deletion mutants, namely Δ1–215, Δ216–393, Δ394–511, and Δ512–861, displayed enhanced binding to caspase-8 (Supplementary Figure S1B). Finally, GST pull-down assays indicated that H109–393 was sufficient to bind to caspase-8 (Figure 1h). Thus, the DOC domain (216–393) alone was insufficient to bind to caspase-8, and the region between 109 and 393 from HECTD3 was necessary and sufficient for caspase-8 binding.
Caspase-8 contains two death effector domains (DEDs) and two caspase domains (p18 and p10) (Figure 1a). DEDs are responsible for the caspase-8 recruitment into DISC through FADD. To map the caspase-8 domain responsible for the association with HECTD3, we expressed the DED and caspase domains of caspase-8 as GST-fusion proteins in HEK293T and performed the GST pull-down assay. Our experimental results indicated that either DED1 or DED2 is sufficient to interact with HECTD3 by performing the GST pull-down assay (Figure 1i and Supplementary Figure S1C). Another DED domain-containing protein FLIPL does not interact with HECTD3 (Supplementary Figure S1D).
HECTD3 ubiquitinates caspase-8 with K63-linked polyubiquitin chains
As shown in Figure 2a, HECTD3 significantly increased caspase-8 polyubiquitination compared with the vector control. It is noted that HECTD3 does not affect the steady-state level of pro-caspase-8 in HEK293T cells, indicating that overexpression of HECTD3 does not target pro-caspase-8 for degradation (Figure 2a). It is possible that HECTD3 modifies caspase-8 with non-K48-linked polyubiquitin chains. The linkage of caspase-8 polyubiquitin chains mediated by HECTD3 was analyzed by using linkage-specific antibodies that specifically recognize K48- or K63-linked polyubiquitin chains under denaturing conditions.31 HECTD3-mediated caspase-8 ubiquitination could be detected by the antibody against K63-linked polyubiquitin chains, but not by the antibody against K48-linked polyubiquitin chains (Figure 2b). To further confirm this result, different ubiquitin mutants including K48-only, K63-only, K48R, K63R, and K0 were used. Consistently, HECTD3-mediated caspase-8 polyubiquitination was supported by WT, K63-only, or K48R Ub, but not by K48-only, K63R, or K0 Ub (Figure 2c). These results clearly indicate that HECTD3 ubiquitinates caspase-8 with K63-linked polyubiquitin chains. Finally, we demonstrated that endogenous ubiquitination of caspase-8 is decreased when endogenous HECTD3 is knocked down in MDA-MB-231 (Supplementary Figure S2E). In contrast to the CUL3-based E3 ligase,11 HECTD3-mediated caspase-8 ubiquitination was not induced by TRAIL (Supplementary Figure S2F).
The catalytic cysteine residue of HECTD3 for caspase-8 is C823
Almost all HECT-type E3 ligases contain a C-terminal catalytic HECT domain with a conserved catalytic cysteine (Cys) located in ‘HTCFN’ box32 (Figure 2d). The catalytic Cys residue is conserved in the HECTD3, although HECTD3 does not have such a box. When HECTD3 C823 was mutated into A, the E3 ligase activity was completely abolished as determined by performing the in vitro HECTD3 self-ubiquitination assay (Figure 2e). Consistently, ubiquitination of caspase-8 by HECTD3 was decreased by the C823A mutation in HEK293T cells (Figure 2f). As HECTD3-C823A appeared to still increase the ubiquitination of caspase-8 compared with the vector control (Figure 2f), we tested whether other C-residues contribute to the caspase-8 ubiquitination. Six other C-residues in the HECT domain were mutated to A, and their E3 ligase activities toward caspase-8 in HEK293T were assessed. Compared with WT and other mutants, the C744A and C744, 823A double mutants efficiently decreased caspase-8 polyubiquitination by HECTD3 (Figure 2f and Supplementary Figure S2A). C744 is conserved in several other HECT-type E3 ligases (Figure 2d). Mutation of five other C residues into A in the HECT domain did not affect the polyubiquitination of caspase-8 by HECTD3 (Supplementary Figure S2A).
HECTD3 ubiquitinates pro-caspase-8 at K215
To identify which lysine (K) residue of caspase-8 is ubiquitinated, different caspase-8 mutants with DEDs (these mutants can interact with HECTD3) were evaluated for HECTD3-mediated ubiquitination. HECTD3 ubiquitinated WT caspase-8, C1–391, but not C1–212 (Supplementary Figure S2B), implying that the HECTD3-mediated caspase-8 ubiquitination sites may be located between 213 and 391. HECTD3 did not efficiently ubiquitinate caspase-8Δ213–233 (Supplementary Figure S2C). There is only one K (215) residue in this region; therefore, the caspase-8-K215R mutant was generated. HECTD3 indeed did not ubiquitinate this mutant (Figure 2g), although the K215R mutation did not affect the interaction between caspase-8 and HECTD3 (Supplementary Figure S2D).
It is well known that caspase-8 cleavage occurs at D384 followed by D210 and D216.33, 34 To test whether caspase-8 cleavage has a role in HECTD3-mediated caspase-8 polyubiquitination, we measured the ubiquitination of an inactive caspase-8 mutant C377A that prevents caspase-8 self-cleavage events.35 As shown in Supplementary Figure S3A, the cleaved caspase-8 band is detected when WT caspase-8 is overexpressed; however, it is undetectable when caspase-8 C377A is overexpressed. Consistently, the basal level of ubiquitination for caspase-8(C377A) is higher than WT caspase-8. Nevertheless, HECTD3 increased the ubiquitination of both WT caspase-8 and caspase-8 (C377A) mutant, suggesting that HECTD3 ubiquitinates pro-caspase-8 independent of its cleavage.
Depletion of HECTD3 increases caspase-8 activation and apoptosis induced by the extrinsic apoptosis pathway
To test if HECTD3 regulates the caspase-8 activation through ubiquitinating caspase-8, HECTD3 was stably knocked down by shRNA in the HeLa cervical cancer cell line (Figure 3a) and MDA-MB-231 breast cancer cell line (Figure 3b). As caspase-8 is mainly activated by the extrinsic apoptotic pathway, stable HECTD3 knockdown cells were treated with different dosages of TRAIL. HECTD3 depletion promotes the caspase-8 cleavage (Figures 3a and b) and activity (Supplementary Figure S3B) in the presence of TRAIL in a dose-dependent manner. In addition, HECTD3-depleted cells showed increased apoptosis as indicated by the increase of cleaved caspase-3 and PARP in the presence of TRAIL (Figure 3a). Similar results were observed in both HeLa and MDA-MB-231 treated with the Fas antibody and TNFα (Figures 3a and b). Transient knockdown of HECTD3 by two different siRNAs also sensitized HeLa cells to TRAIL-induced caspase-8 activation and apoptosis (Supplementary Figure S4A).
The enhancement of TRAIL-induced apoptosis by HECTD3 depletion was further assessed by the Annexin V/7-AAD staining, the cell viability assay, and the clonogenic assay. As shown in Figure 3c, HECTD3 knockdown in MDA-MB-231 significantly increased the percentage of Annexin V-positive apoptotic cells. In addition, the cell viability of MDA-MB-231 HECTD3sh cells was significantly less than that of Lush cells after the TRAIL treatment (Figure 3d). The clonogenic assay showed that HECTD3 knockdown markedly decreased the colony formation in the presence of TRAIL (Figure 3e).
HECTD3 decreases the caspase-8 recruitment and inhibits its activation
To investigate the potential mechanism by which HECTD3 inhibits caspase-8 activation, we examined TRAIL-induced caspase-8 recruitment into DISC. As shown in Figure 4a, more endogenous caspase-8 proteins were recruited into DISC when endogenous HECTD3 was knocked down in MDA-MB-231. HECTD3 itself was not recruited into DISC (Figure 4a).
To test whether HECTD3 inhibits caspase-8 cleavage and activity by ubiquitinating caspase-8, TRAIL-mediated caspase-8 cleavage and activity in HEK293T was measured. WT HECTD3 significantly inhibited TRAIL-induced caspase-8 cleavage (Figure 4b, lane 5 compared with lane 4) and activity (Supplementary Figure S3C); however, the catalytic inactive HECTD3m (lane 6) did not inhibit TRAIL-induced caspase-8 cleavage and activity (Supplementary Figure S3C). Compared with that of WT caspase-8 (Figure 4b, lane 5), the cleavage of caspase-8-K215R induced by TRAIL (Figure 4b, lane 11) could not be blocked by HECTD3 overexpression. Consistently, the caspase-8 activity is neither reduced by HECTD3 (Supplementary Figure S3C). TRAF2 has been shown to target caspase-8 p18 for proteasomal degradation.14 The proteasome inhibitor MG132 only slightly increased the p18 protein level in MDA-MB-231 cells treated with TRAIL (Supplementary Figure S3D), suggesting that HECTD3 does not target p18 for proteasomal degradation.
HECTD3 overexpression was evaluated as a potential rescue of the HECTD3 knockdown-induced caspase-8 cleavage and apoptosis increase in the presence of TRAIL. HECTD3 shRNA-resistant WT HECTD3R and HECTD3mR were stably expressed in the HECTD3-depleted MDA-MB-231 cells. As shown in Figures 4c and d, the expression of HECTD3R protected MDA-MB-231 cells from TRAIL-induced caspase-8 cleavage and cell viability decrease; however, the catalytic inactivated HECTD3m R did not. These results not only suggest that the pro-apoptosis phenotype caused by HECTD3 shRNA is not an off-target effect but also further confirm that the E3 ligase activity of HECTD3 is required to suppress TRAIL-induced apoptosis.
HECTD3 promotes cell survival through caspase-8
Previously, we demonstrated that HECTD3 promotes cancer cell survival partially through MALT1.29 To test if HECTD3 decreases TRAIL-induced apoptosis through caspase-8, a rescue experiment was performed by depleting caspase-8 and HECTD3 together using siRNA in both MDA-MB-231 and HeLa cells followed by treatment of the cells with TRAIL. Caspase-8 ablation almost completely abrogates HECTD3 siRNA-induced apoptosis increase in the presence of TRAIL, as determined by measuring caspase-8, -3, -7, and PARP cleavage as well as cell viability (Supplementary Figure S4B-E). In contrast, caspase-9 ablation has no significant effect on HECTD3 siRNA-induced apoptosis increase in the presence of TRAIL (Supplementary Figure S4F).
HECTD3 is frequently overexpressed in breast cancers
HECTD3 mRNA is strongly expressed in the human salivary gland, liver, thyroid, kidney, muscle, and brain, but weakly expressed in the ovary, prostate, skin, cervix, and mammary gland (Supplementary Figure S5A). The HECTD3 expression in different human tissues was further validated by immunohistochemical analysis (IHC) (Supplementary Figure S5B-C and data not shown).
As HECTD3 mRNA is weakly expressed in normal mammary glands, the HECTD3 mRNA levels were measured by qRT-PCR in 30 breast cancer cell lines. Compared with four nontransformed breast cell lines (HMEC, 184A1, MCF10A, and 184B5), 19 of 30 (63.3%) of breast cancer cell lines overexpress HECTD3 by at least twofold (Figure 5a).
To further validate the qRT-PCR results, northern blot analyses for HECTD3 were performed in several breast cancer cell lines. As expected, a single ∼3.6-Kb mRNA band was detected in all samples examined (Supplementary Figure S6A). The HECTD3 mRNA levels are higher in cancer cell lines than those in immortalized cell lines (Supplementary Figure S6A). To test if the HECTD3 gene undergoes genetic alterations in breast cancer, the DNA copy-number of HECTD3 in 31 breast cancer cell lines was evaluated. Compared with the genomic DNA from normal human cells and three immortalized cell lines (MCF10A, 184A1, and 184B5), 15 of the 31 breast cancer cell lines (48.4%) showed gains in gene copy-number for HECTD3 (Supplementary Figure S6B). There is a significant correlation between gain of the HECTD3 gene copy-number and the HECTD3 mRNA overexpression in breast cancer cell lines (Supplementary Table S1).
The endogenous HECTD3 protein levels in breast cancer cell lines were measured by western blotting (WB). The HECTD3 protein band was detected at 96 KDa in all samples (Figure 5b). In agreement with the mRNA expression levels, the HECTD3 protein levels were found to be low in the HMEC nontransformed breast cell line but elevated in breast cancer cell lines.
To confirm whether HECTD3 is also overexpressed in breast tumors, the HECTD3 mRNA levels were measured in a panel of breast tumors by qRT-PCR. The average HECTD3 mRNA levels at normal breast and breast tumors are plotted in Figure 5c. Compared with normal breast tissues, breast tumors showed significantly elevated expression of the HECTD3 mRNA (P<0.05, t-test). Furthermore, HECTD3 protein expression was also examined in normal breast tissues and breast carcinomas by IHC (Figure 5d). The anti-HECTD3 antibody was validated to specifically detect the HECTD3 protein by IHC (Supplementary Figure S5B). The majority (86%, 36/42) of normal breast tissue sample and normal breast tissues adjacent to invasive carcinomas are negative for HECTD3 expression. In contrast, HECTD3 protein is overexpressed in 42 of 79 (53%) of breast carcinomas (P=0.0002, Fisher’s exact test) and localized in both the cytoplasm and the nucleus.
Discussion
E3 ligases, such as Mdm2 and IAPs, have been well documented to inhibit apoptosis. The present data support a model that a novel HECT type E3 ligase HECTD3 inhibits the extrinsic apoptosis pathway at least partially through ubiquitinating caspase-8 with K63-linked polyubiquitin chains in human cancers. HECTD3 is frequently overexpressed in breast cancer cell lines and tumors at both mRNA and protein levels. HECTD3 depletion sensitized cancer cell lines to TRAIL, TNFα, and Fas antibody induced apoptosis. Ectopic expression of HECTD3 conferred MDA-MB-231 TRAIL resistance in an E3 ligase activity-dependent manner. HECTD3 interacts with caspase-8, ubiquitinates caspase-8 with K63-linked polyubiquitin chains at K215, and reduces its recruitment into DISC for activation. Taken together, the HECTD3 E3 ligase overexpression in breast cancers promotes cancer cell survival from extrinsic apoptotic stimuli at least partially through ubiquitinating caspase-8 with K63-linked polyubiquitin chains (Supplementary Figure S7).
HECTD3 interacts with caspase-8 through the DOC-DED interactions (Figure 1). Several other E3 ubiquitin ligases, such as APC10, CUL7, PARC, and HERC2, contain a DOC domain.36 The DOC domain of APC10 contributes to substrate recognition for the anaphase-promoting complex (APC) E3 ligase,37 although the DOC-binding partner is unknown. Here, we demonstrated that the DOC domain of HECTD3 binds to the DED domain of caspase-8 (Figure 1) and DD domain of MALT1.29 It would be interesting to test whether other DOC domain-containing E3 ligases also interact with caspase-8. There are several other DED domain-containing proteins, such as FLIP, FADD, PEA-15, and DEDD.38 HECTD3 did not interact with FLIPL. These results indicate that the interaction between HECTD3 and caspase-8 is specific.
The data presented in this study indicate for the first time that HECTD3 promotes cell survival partially through ubiquitinating caspase-8 with K63-linked polyubiquitin chains at K215. Ubiquitination-mediated inactivation of caspases has been long linked to apoptosis resistance.39 Ditzel et al.40 reported that the DIAP1 E3 ligase inactivates effector caspase (drICE) through nondegradative polyubiquitination in Drosophila. The current study provides the first evidence that HECTD3-mediated caspase-8 K63-linked polyubiquitination suppresses the activation of caspase-8. Caspase-8 is well known to be recruited into DISC for self-cleavage and activation. Depletion of HECTD3 increases the caspase-8 recruitment and activation. In contrast to the CUL3-mediated caspase-8 polyubiquitination at the C-terminus, HECTD3-mediated caspase-8 polyubiquitination at K215 decreases its activation. This mechanism is also different from TRAF2-mediated K48-linked polyubiquitination on the large catalytic domain of caspase-814 and TRIM13-mediated K63-linked caspase-8 polyubiquitination, which results in caspase-8 activation during ER stress.41
Yu and his colleagues previously reported that HECTD3 is the E3 ligase for Tara.27 An N-terminal-truncated HECTD3 (284–861) was shown to interact with exogenous Tara and to target exogenous Tara for ubiquitin-mediated proteasomal degradation.27 In another report, Zhang et al.28 identified the Syntaxin-8 protein as a HECTD3 interacting protein by yeast two-hybrid screening and demonstrated that HECTD3 increases the Syntaxin-8 ubiquitination in HEK293T cells. Syntaxin-8 is a vesicle trafficking protein associated with neurodegenerative diseases.42 Thus, it cannot be completely excluded the possibility that HECTD3 promotes cell survival partially by targeting Tara and/or Syntaxin-8 for degradation. Our previous study showed that HECTD3 stabilizes MALT1 and protects cancer cells from cisplatin-induced apoptosis.29 MALT1 and caspase-8 are known to form a protein complex.30 Thus, it seems that HECTD3 functions partially through both MALT1 and Caspase-8. It is also possible that HECTD3 possesses other unidentified functions by regulating Tara, Syntaxin-8, or other substrates.
HECTD3 is a pro-survival protein overexpressed in breast cancers. Whether the HECTD3 expression level is an ideal biomarker for breast cancer diagnosis and prognosis requires larger scale IHC studies in the future. The future HECTD3-targeted therapy should combine with other therapies such as TRAIL and cisplatin.
Materials and Methods
Cell culture
MDA-MB-231 was cultured in DMEM supplemented with 2 mM L-glutamine, 0.1 mM non essential amino acid, 1.5 g/l sodium bicarbonate, 1 mM sodium pyruvate and 10% FBS. HeLa was cultured in DMEM supplemented with 5% FBS and 1% PS.
Antibodies
The anti-HECTD3 rabbit polyclonal antibody was described in our previous study.29 The anti-HECTD3 antibody was validated by the exogenous expressed HECTD3 protein by using a standard WB protocol (Supplementary Figure S5B). The anti-β-actin mouse monoclonal antibody, the anti-Flag rabbit polyclonal antibody, the anti-PARP, anti-caspase-7, anti-cl-caspase-7, anti-caspase-8, and anti-caspase-3 antibodies are from Cell Signaling (Danvers, MA, USA). The rabbit anti-caspase-8 antibody is from R&D systems. The anti-DR4 antibody is from DIACLONE (Tepnel Company, France). The anti-DR5 antibody is from ProSci Inc. (Poway, CA, USA). The anti-FADD, anti-Ub, anti-ERα, and anti-HA antibodies are from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-GST antibody and anti-Flag M2 monoclonal antibody are from Sigma (St. Louis, MO, USA). Ubiquitin linkage-specific antibodies (Apu2.07 and Apu3.A8) are gifts from Dr. Dixit, V. M. in Genentech.31
Plasmids
The endogenous HECTD3 protein is about 96 KDa, which is consistent with the open reading frame of the HECTD3 mRNA sequence (NM_024602.5) (Supplementary Figure S8). The full-length HECTD3 gene was cloned into the pLenti6/V5-D vector as described.29 MDA-MB-231 cells were transduced with WT HECTD3 and mutant HECTD3-C774, 823A respectively. The cell populations were obtained after selection with 10 μg/ml blasticidin. The deletion mutants of Flag-HECTD3 (Δ1–215, Δ109–393, Δ216–393, Δ394–511, Δ1-511, and Δ512–861) and HECTD3 mutants were generated by a PCR-based approach. To generate HECTD3 shRNA (Hsh)-resistant HECTD3 cDNA, we silently mutated 10 nucleotides within the shRNA target sequence (from 5′-GCGGGAACTAGGGTTGAAT-3′ to 5′-AAGAGAGTTGGGTCTAAAC-3′). Different regions of HECTD3 were cloned into pEBG and pGEX-6p-1 vectors to produce GST-fusion proteins in mammalian cells and in E.coli, respectively. The pcDNA3-caspase-8 (NM_001228) plasmid is a gift from Dr. Gabriel Nuñez from University of Michigan. pEF-IRES-P-HA-Ub and Ub mutants (K0, K48-only, K63-only, K48R, and K63R) are gifts from Dr. James Zhijian Chen (University of Texas Southwestern Medical Center).43
Transfection
All transfections for plasmids and siRNAs were performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instruction. All chemically synthesized siRNAs were purchased from Ambion (Austin, TX, USA) and transfected at 25 nM final concentration. The siRNA target sequences for the human HECTD3 gene are 5′- GCGGGAACTAGGGTTGAAT-3′. The sequence was chosen for preparing the HECTD3 shRNA construct in the pSIH1-H1-Puro shRNA vector. The siRNA target sequence for the human caspase-8 gene is 5′- GATCAGAATTGAGGTCTTT- 3′.
Ubiquitination assays
HEK293T cells were transiently transfected with HA-Ub and other plasmids as necessary in six-well plates. Two days after transfection, the cells were collected in 150 μl SDS lysis buffer (50 mM Tris-Cl, pH 6.8, 1.5% SDS). The samples were boiled for 15 min. One hundred microliters of protein lysate was diluted with 1.2 ml EBC/BSA buffer (50 mM Tris-Cl, pH 6.8, 180 mM NaCl, 0.5% CA630, 0.5% BSA) and incubated with anti-Flag M2-agarose overnight at 4 °C with rotation. The beads were collected by centrifugation at 10 000 × g for 30 s at 4 °C and washed three times with 1 ml ice-cold EBC/BSA buffer. Proteins were resuspended with 30 μl of 3 × SDS sample loading buffer and analyzed by WB. Ubiquitinated caspase-8 was detected by an anti-HA antibody.
The HECTD3 self-ubiquitination assay was performed with recombinant-purified GST-HECTD3, GST-HECTD3-C823A, E1, E2 (UbcH5b), Ub, and ATP in a 15-μl system. The reactions were incubated at 30 °C for 3 h. The ubiquitinated HECTD3 proteins were detected by WB using the anti-Ub antibody.
DISC formation
MDA-MB-231 cells (∼1 × 107 Lush and Hsh) were grown in a 15-cm dish and collected in 2 ml complete DMEM. The recombinant human soluble Flag-tagged TRAIL (1 μg, Alexis) and the anti-Flag M2 agarose (4.6 μg) were premixed for 15 min on ice in a 1.5 ml Eppendorf tube. The cells were added into the tube for 30 min at 37 °C with gentle shaking. The reaction was stopped by the addition of 10 ml ice-cold PBS. The cells were immediately collected (400 g, 5 min, 4 °C), washed with 1 ml ice-cold PBS, and lysed in 1 ml lysis buffer for 15 min on ice. The lysate was centrifuged twice at 16 000 g for 10 min at 4 °C. In unstimulated controls, 1 μg Flag-TRAIL and the anti-Flag M2 agarose were directly added into the cell lysate. Beads were recovered by centrifugation, washed five times with 500 μl lysis buffer, and subjected to WB.
HECTD3 mRNA analysis
The HECTD3 mRNA levels were examined by qRT-PCR as described in our previous studies.44, 45 Primer sequences for HECTD3 are 5′- GGGCTACGAGACCACAGACG -3′ and 5′- GCAAGGCCAAGAGGGACAC -3′. All qPCRs were performed in duplicate. GAPDH and β-actin were used as loading controls. HECTD3 overexpression is defined when the ratio in a cancer sample is larger than two compared with the average ratio in all normal samples. cDNA from 48 breast tissues (BCRT102) and normal tissues (HMRT102) was purchased from Origene (Rockville, MD, USA).
Immunohistochemical staining
To confirm the specificity of the anti-HECTD3 antibody for IHC, the HEK293T cells (transfected with HECTD3 and an empty vector control) were stained by using an indirect biotin avidin diaminobenzidine detection system according to the routine IHC procedure (Supplementary Figure S5B). After validating the anti-HECTD3 antibody for IHC, normal breast and breast carcinoma tissue microarrays were purchased from US Biomax, Inc (BC08022 and BC08014). In total, 42 different normal or adjacent (1.5 cm away from the tumor) normal breast tissues and 79 breast carcinomas were stained. Slides were baked at 60 °C for 2 h and underwent antigen retrieval procedures before regular IHC. The anti-HECTD3 primary antibody was used at a dilution of 1 : 100. Immunoreactivity for HECTD3 was interpreted by two pathologists without previous knowledge of the clinicopathologic parameters. Immunostained slides were evaluated by light microscopy, and the IHC signal was scored using the ‘Allred Score’ method.46
Immunoprecipitation and GST pull-down
Immunoprecipitation using anti-Flag M2-agarose (Sigma, #A2220) and GST pull-down experiments have been described in our previous studies.23 The anti-HECTD3 rabbit polyclonal antibody was used to immunoprecipitate the endogenous HECTD3 proteins from HeLa. The rabbit IgG was used as the negative control.
In vitro translation
Caspase-8 and the caspase-3 proteins were synthesized from the pcDNA3-Caspase-8 or -3 plasmids using the TNT Quick Coupled Transcription/Translation Systems (Promega, Madison, WI, USA) in the presence of methionine, according to the manufacturer’s protocol.
Apoptosis
Cells were transfected with the HECTD3 siRNA and control siRNA for 2 days. If applied, different concentrations of TRAIL were added for indicated time. The cell viability was measured by performing the SRB assay as described in our previous study.44 Cleaved PARP and cleaved caspase-3, -7, -8 protein levels were detected by WB. Annexin V/7-AAD (BD, Franklin Lakes, NJ, USA) staining was performed according to the manufacturer’s protocol.
Statistical analysis
The cell viability experiments were conducted in triplicate and repeated at least once. The data were pooled to generate means±S.D. and analyzed by Student’s t-test. The HECTD3 protein expression in tumors and the correlation between the HECTD3 gene copy-number gains and mRNA overexpression were analyzed by Fisher’s exact test. P-values of <0.05 were considered to be significant.
Abbreviations
- APC:
-
anaphase-promoting complex
- C:
-
cysteine
- DED:
-
death effector domains
- DISC:
-
death-inducing signaling complex
- DOC:
-
destruction of cyclin
- HECT:
-
homologous to E6-AP COOH terminus
- IAP:
-
inhibitor of apoptosis
- IHC:
-
immunohistochemical analysis
- IP:
-
immunoprecipitation
- K:
-
lysine
- TRAIL:
-
TNF-related apoptosis-inducing ligand
- Ub:
-
ubiquitin
- WB:
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western blotting
- WT:
-
wild type
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Acknowledgements
We thank Dr. Gabriel Nuñez from University of Michigan, Dr. James Zhijian Chen from University of Texas, Dr. Chunghong Yan from Albany Medical College, and Dr. Dixit, V. M. from Genentech for reagents. Drs. Gang Liu and Guoning Liao from Albany Medical College provided technical support. This study was supported in part by National Key Basic Research Program of China (No. 2013CB910900), grants from National Nature Science Foundation of China (81120108019, 81072162, and U1132605), and a grant from Yunnan Province High-Profile Scientist Program (2010CI114).
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Li, Y., Kong, Y., Zhou, Z. et al. The HECTD3 E3 ubiquitin ligase facilitates cancer cell survival by promoting K63-linked polyubiquitination of caspase-8. Cell Death Dis 4, e935 (2013). https://doi.org/10.1038/cddis.2013.464
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DOI: https://doi.org/10.1038/cddis.2013.464
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