Abstract
Central Europe during the Last Glacial Maximum (LGM) was dominated by polar desert and steppe-tundra biomes. Despite this, a human presence during this time period is evident at several locations across the region, including in Switzerland, less than 50 km from the Alpine ice sheet margin. It has been hypothesised that such human activity may have been restricted to brief periods of climatic warming within the LGM, but chronological information from many of these sites are currently too poorly resolved to corroborate this. Here we present a revised chronology of LGM human occupation in Switzerland. AMS radiocarbon dating of cut-marked reindeer (Rangifer tarandus) bones from the sites of Kastelhöhle-Nord and Y-Höhle indicates human occupation of Switzerland was most likely restricted to between 23,400 and 22,800 cal. BP. This timeframe corresponds to Greenland Interstadial 2, a brief warming phase, supporting the hypothesis that human presence was facilitated by favourable climatic episodes. Carbon, nitrogen and sulphur stable isotope analysis of the fauna provides palaeoenvironmental information for this time period. These findings contribute to our understanding of human activity in ice-marginal environments and have implications for understanding cultural connections across central Europe during the LGM.
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Introduction
Switzerland during the Last Glacial Maximum (LGM, c. 26,500–19,000 BP1,2) was almost entirely covered by ice, with only a small region north of the Jura Mountains remaining ice-free3. Despite this, human occupation during this period is evidenced from two locations in Switzerland; Kastelhöhle-Nord and Y-Höhle4,5 (Fig. 1). Previous radiocarbon dates from these sites indicate that people were present between 24,000 and 22,000 cal. BP5,6. This broadly corresponds to a period of global glacial advance, when a cold-arid climate existed in Europe north of the Alps, and mean annual temperatures were as much as 15 °C lower than present-day7,8. However, short-term climatic fluctuations are also evident during this time interval, including a brief warming event associated with Greenland Interstadial 2 (GI-2, c. 23,300–22,800 BP9), which may have facilitated human occupation in Switzerland6. If this hypothesis is to be tested, more precise chronological and environmental information regarding the human activity at Kastelhöhle-Nord and Y-Höhle is required. This information is also crucial to situate the Swiss LGM archaeological record within the wider debate surrounding the extent of human occupation in central Europe during the LGM, the environmental conditions under which it occurred, and the extent to which different cultural groups may have interacted6. Here we present a revised chronology of human occupation in Switzerland during the LGM based on new radiocarbon dates, and environmental interpretations based on stable isotope analysis (carbon, nitrogen and sulphur) of reindeer bone collagen.
The LGM is a time-transgressive period within Marine Isotope Stage 2 (MIS2). The nomenclature is variously used to refer to the episode during which maximum global ice volume was reached, when minimum global sea level occurred, or when the lowest oxygen isotope values (δ18O) are recorded in marine or ice core records10,11. Associated time intervals range from c. 25,000 to 18,000 BP, but broadly centre upon 21,000 BP10,11. Within Switzerland, the last glacial advanced occurred sometime after 30,000 BP1. Local geomorphological evidence and speleothem δ18O minima suggest maximum ice extent may have been reached between 26,500 and 22,000 BP, while the onset of rapid ice decay started by c. 19,000 BP1,2,12,13. Climate models and proxy data indicate a cold dry climate existed north of the Alps during this time period, while the formation of Alpine speleothems suggests warm air masses from the south delivered precipitation to the northern Alpine slopes2,7,8. North of the mountains, continuous periglacial permafrost conditions existed, with tundra and steppe landscapes dominated by graminoid and forb vegetation14,15. Nonetheless, the Alpine LGM was punctuated by sub-millennial climate fluctuations related to changing temperature and precipitation regimes, which influenced local environmental conditions and ice extent2,16,17,18. Within Switzerland a positive excursion in speleothem δ18O occurred between 23,230 ± 37 BP and 22,759 ± 47 BP2, linked to changing precipitation patterns, and this appears to have coincided with a phase of recession in the Alpine ice sheet18. The timings of these local environmental changes matches with the Greenland warm period of GI-2 (23,290 ± 596 BP to 22,850 ± 573 BP9), within dating uncertainties. This suggests that the warming in Switzerland may have been associated with continental-scale processes. The Greenland record of GI-2 is interrupted by a brief climatic cooling lasting around 200 years (Greenland Stadial 2.2) and centring on approximately 23,100 BP9. The expression of this event in the Swiss record is less clear but may occur between 22,971 ± 46 BP and 22,888 ± 50 BP2.
The link between GI-2 and human activity in Switzerland has previously been postulated6. However, chronological resolution is currently poor and archaeological evidence is sparse. The dated cut-marked reindeer (Rangifer tarandus) bones from Kastelhöhle-Nord (n = 3) and Y-Höhle (n = 1) represent the only directly dateable archaeological evidence from this time period in Switzerland5,6. Both cave sites are located within 10 km of one another, on the north-eastern edge of the Jura Mountains, less than 50 km from the maximum ice sheet extent. Lithics found in association with the reindeer bones at Kastelhöhle-Nord have been attributed to the Badegoulian/Early Magdalenian4,6, a Late Upper Palaeolithic culture found in western-central Europe. In contrast, the cut-marked reindeer bone from Y-Höhle was found in a secondary context with no association to other archaeological remains5. Lithics of Badegoulian character are also found in Germany (Wiesbaden-Igstadt, Rhineland, and Gera-Zoitzberg, Thuringia)6,19, while broadly contemporaneous evidence of human activity is also present in southern Germany (Mittlere Klause), and further east in Austria (Kammern-Grubgraben and Langmannersdorf), the Czech Republic (Stranska Skala IV) and possibly Poland (Deszczowa Cave and Zytnia Skala)20,21,22,23. The latter are usually attributed to Epigravettian cultures of eastern central Europe21. Hence, a great amount of uncertainty surrounds the cultural and chronological relationship between these sites, as well as the environmental conditions under which they were occupied, but it has been postulated that brief climatic warming events may have facilitated the periodic expansions of human populations into northern central Europe, and cultural contact across it6.
Radiocarbon and stable isotope analyses
One of the most significant developments in the radiocarbon dating of bone collagen in the last 20 years has been the routine inclusion of an ultrafiltration step in the sample pre-treatment procedure at some laboratories24,25. Ultrafilters remove the low molecular weight fraction from the sample, thus more thoroughly removing modern organic contaminants24. However, not all studies conclude that ultrafiltration is a necessary part of the radiocarbon bone sample preparation procedure26,27, and without stringent cleaning protocols their use may risk introducing additional contaminants into the sample24,28,29. Certainly, it is necessary to employ rigorous quality checks to the radiocarbon dating process, regardless of the method of sample preparation used30,31. Following such quality controls, the application of the ultrafiltration method has been shown to have particularly significant implications for the dating of bone collagen from Palaeolithic contexts, where the contribution of even the smallest amount of contamination can have significant consequence for the obtained date32,33,34. Considering these developments, it is timely to re-evaluate the radiocarbon chronology of human occupation in Switzerland during the LGM. As such, the four previously dated cut-marked reindeer bones from Kastelhöhle-Nord intermediate horizon and Y-Höhle were targeted for radiocarbon dating using the current ultrafiltration sample preparation methodology and quality control criteria used by the Oxford Radiocarbon Accelerator Unit (ORAU).
Bone collagen can also be analysed for its stable isotope compositions, which are powerful tools for investigating past ecology and terrestrial environments, and have been employed widely in Late Pleistocene archaeological research35,36,37,38,39,40,41. In this study we use carbon (δ13C), nitrogen (δ15N) and sulphur (δ34S) isotope ratios in reindeer bone collagen to examine paleoenvironmental conditions during the period of human activity at Kastelhöhle-Nord and Y-Höhle. Carbon isotope ratios are largely determined by dietary behaviour, and reindeer δ13C values are known to be systematically enriched in comparison to other herbivore species due the consumption of lichen42,43. Dietary δ13C values are also influenced by atmospheric CO2 δ13C value and concentration, and by environmental parameters such as temperature, moisture availability and density of vegetation cover35,44,45,46. Bone collagen δ15N values are linked to both dietary specialisation41,47,48 and to environmental conditions29,30,31,32. Soil and plant δ15N values are influenced by climatic variables such as temperature and precipitation, mediated through soil processes49,50. In particular, soil maturity, nutrient availability and microbial activity have been cited as having a strong control on herbivore bone collagen δ15N values, with permafrost and proximity to ice sheets likely playing a significant role in the generally low δ15N values observed in Late Pleistocene Europe36,37,38,39. Bone collagen δ34S values largely reflect the varying forms of sulphur present in soil, which is related to mineral input from the underlying geology, deposition of sulphates from groundwater and the atmosphere, and microbially-mediated fractionation processes51. As such, δ34S values are spatially variable and often considered a tool for exploring mobility and landscape utilisation38,52,53. However, bone collagen δ34S values may also hold significant promise as a palaeoenvironmental proxy representing changing hydrological and microbial processes51,54,55. In this study, bone collagen stable isotope analyses are performed on the 4 dated specimens, plus a further 7 reindeer bones from Kastelhöhle-Nord intermediate horizon.
Results
Collagen preservation at both sites was excellent; all samples produced collagen yields between 2.9% and 14.3%, which is comparable to the amount of collagen produced from modern samples prepared using the same methodology56. All samples had C:N atomic ratios between 3.2 and 3.4, and carbon and nitrogen content between 41–45% and 14–16%, respectively, comparable to in vivo collagen57,58. Sulphur content ranged between 0.14–0.19%, while C:S and N:S atomic ratios were between 625–785 and 190–245, indicating good sample integrity59.
The new radiocarbon determinations fall within the range of the previously published dates, but indicate a significantly shorter duration of human activity (Table 1, Fig. 2). Two new radiocarbon dates were produced on sample UPN-223 as an internal laboratory quality check. As the results are statistically identical an error-weighted mean of 19,121 ± 60 14C BP shall be used for this sample in the subsequent discussion of the results. The overall range represented by the new radiocarbon dates is 19,300 ± 90 14C BP to 19,121 ± 60 14C BP (Table 1). A Bayesian statistical modelling approach was applied to the Kastelhöhle-Nord samples assuming that the three samples represent the same phase of activity at the site. The results show that occupation occurred between 23,450 and 22,733 cal. BP (95% probability). This compares to the date from Y-Höhle of 23,531–22,963 cal. BP (95% probability) (Fig. 3).
For stable isotope analyses, each sample was analysed in duplicate and reproducibility was better than ±0.1‰ for δ13C, ±0.2‰ for δ15N and ±0.3‰ for δ34S. Reindeer δ13C and δ15N values from the Kastelhöhle-Nord intermediate horizon and Y-Höhle ranged from –19.7‰ to –18.5‰ and +2.5‰ to +3.9‰ respectively (Fig. 4, Supplementary Table S1). These values are typical of Late Pleistocene reindeer in Europe36,37,38 and largely reflect the animals’ behavioural ecology and dietary specialisation for lichen, underlain by environmental influences. Reindeer δ34S values from the Kastelhöhle-Nord intermediate horizon ranged from –10.8‰ to –7.5‰, while the δ34S value for the Y-Höhle sample was measured as –12.6‰ (Fig. 4, Supplementary Table S1).
Discussion
Chronology of human settlement in Switzerland and central Europe during the LGM
Based on the new radiocarbon evidence, human settlement in Switzerland took place between c. 23,400 and 22,800 cal. BP. While the possibility of human presence in Switzerland during the LGM and early post-LGM outside of this time interval cannot be excluded, currently no evidence exists to support this. The radiocarbon age range broadly coincides with the end of a phase of Alpine glacier recession (c. 23,500 BP1,18), and with a period of change to Alpine precipitation patterns (23,230 ± 37 BP to 22,759 ± 47 BP2). This phase of Alpine environmental change likely corresponds to Greenland Interstadial 2 (23,290 ± 596 BP to 22,850 ± 573 BP9), although the dating uncertainties do no permit correlation with absolute certainty. Nonetheless, the age range of the faunal radiocarbon dates does appear to correlate well with the local, short-lived episode of climatic amelioration and glacial recession (Fig. 3), suggesting human presence in Switzerland at this time may have been facilitated by environmental change. However, even the smaller uncertainties associated with these local records means that we cannot be certain that the phase of human activity corresponds to the interstadial. This is where the isotope data derived directly from the cut-marked specimens can facilitate our interpretation of local environmental conditions at the time of human activity.
The slightly older date from Y-Höhle compared to Kastelhöhle-Nord (and perhaps rather speculatively, the different δ34S values between the sites), indicates that human activity during this time period was likely not restricted to a single occupation event. Indeed, while the Y-Höhle sample was found in a secondary context with no association to other archaeological remains5, the Kastelhöhle-Nord samples were found in association with a lithic assemblage of 228 artefact5, which could represent multiple periods activity at the site. The impact of the cooler phase that occurred within GI-2 (GS-2.2, dated locally to c. 22,971 ± 46 BP to 22,888 ± 50 BP) cannot be assessed given the short duration of the event relative to the dating uncertainties.
Although sparse, the archaeological record of central Europe after c. 25,000 BP does attest to human activity persisting during the LGM part of MIS 2, at least sporadically6,21. Similarities between the Swiss lithic record and those from Germany (Wiesbaden-Igstadt and Gera-Zoitzberg) and lower Austria (Kammern-Grubgraben) have been interpreted as indicating long-distance contact across western and eastern central Europe during this time, and this is supported by the overlapping radiocarbon chronologies of the sites (with the exception of Gera-Zoitzberg where there is no absolute chronological information available)6,19. More broadly, radiocarbon dating of archaeological material from Mittlere Klause in southern Germany, Langmannersdorf in Austria and Stranska Skala IV in the Czech Republic also span this time interval6,20,22.
Recent chronological investigations at Kammern-Grubgraben (Layers 2–4) have produced a series of 6 ultrafiltered AMS dates ranging from 19,330 ± 70 14C BP to 19,070 ± 60 14C BP (c. 23,400 and 22,850 cal. BP), significantly refining the likely duration of human activity from a previous estimate of 19,380 ± 90 14C BP to 17,350 ± 190 14C BP23. This revised chronology shows striking overlap with the Swiss dates, and with GI-2. By comparison, Wiesbaden-Igstadt, Mittlere Klause, Langmannersdorf and Stranska Skala IV all have radiocarbon chronologies that span this time interval, but with age ranges too broad to evaluate whether a more certain relationship with GI-2 can be established. Indeed, the only ultra-filtered date from Mittlere Klause (OxA-9856 18590 ± 260 14C BP4) was later withdrawn due to concerns over potential contamination60. It would certainly be of interest to re-date this sample and revisit the chronology of other sites to further evaluate the timing of human activity in central Europe during the LGM.
Environmental context of human settlement in Switzerland during the LGM
The δ13C values obtained from the Swiss reindeer samples are characteristic of a diet incorporating a significant proportion of lichen37,38. Lichens are typically enriched in 13C by c. 2–4‰, relative to C3 vascular plants growing within the same environment61, and this enrichment is reflected in reindeer skeletal δ13C, relative to other herbivores with a diet based solely on C3 vascular plants42,43. For many modern reindeer herds lichen is an important food source, particularly in the winter months when reindeer forage for the resource under snow cover62. It is likely that the ability to utilise lichen as a significant dietary component facilitated the survival of reindeer close to the ice sheet margins. Their use of this habitat could also explain the reason for human presence in the region during the LGM. However, it is not possible to evaluate whether reindeer, or indeed people, remained year-round in the vicinity of Kastelhöhle-Nord and Y-Höhle. Both sedentary and long-distance seasonal migratory behaviours are observed in modern reindeer herds63,64,65 and a similar diversity of behaviours has been argued for European Pleistocene populations66,67,68,69. It is therefore possible that human presence in northern Switzerland represents only short-term, perhaps seasonally-restricted, exploitation of local reindeer herds during periods of favourable environmental conditions.
Regardless of whether the reindeer represent a sedentary or migratory population, their δ15N and δ34S values provide information on the environment in which they lived. In the context of the Jura Mountains during the Late Pleistocene, soil maturity has been suggested as the controlling factor on reindeer δ15N isotope ratios, with low values (≤2‰) indicating recently deglaciated, nutrient limited landscapes and higher values (≥3.5‰) indicating reindeer were utilising refugial areas, where soils were more developed38. The majority of the LGM Kastelhöhle-Nord and Y-Höhle reindeer display intermediary δ15N values (+2.5‰ to +3.9‰). We suggest that the observed δ15N values could be produced in an environment where climatic/permafrost conditions limited soil nutrient cycling, which would have produced low environmental δ15N values, but where landscape stability facilitated the development/survival of soils and hardy vegetation enough to sustain reindeer populations. There is a smaller amount of variation in the δ34S values for the Swiss reindeer than in other LGM populations38,70, suggesting the Swiss animals had a more homogenous geographical range. However, it is interesting to note that of the four directly dated samples, the oldest (from Y-Höhle) has the lowest δ34S value (–12.6‰), while the three slightly younger Kastelhöhle-Nord samples have an average δ34S value of –8.2 ± 0.4‰, potentially indicating a temporal change in local environmental conditions or migratory behaviour.
When results are compared to published reindeer collagen stable isotope data from other regions in Europe during the LGM, considerable overlap in δ13C and δ15N values between locations is observed, suggesting reindeer ecology and behaviour plays a significant role in the derived isotopic signatures (Fig. 5, Supplementary Table S2). The highest δ13C values are observed in reindeer from the Swiss and French Jura and the Massif Central in France, which may indicate a greater reliance on lichen as a food source in these environments, or potentially a small altitudinal effect on the carbon isotope signatures71. δ15N values overlap between all regions considered, but the lowest (≤2‰) and highest (≥5‰) δ15N values that are observed in the Middle Rhine region of Germany and southwest France respectively, are absent from the Swiss and French Jura and the Massif Central samples. While the lack of the highest δ15N values suggests an environment in which soil nutrient cycling was limited, probably due to overall low environmental temperatures, the lack of the lowest δ15N values also suggests periglacial processes likely did not dominate the environmental δ15N signature36,38. Significant location-based differences are apparent in the δ34S values (Fig. 5, Supplementary Table S2), although the comparatively small sample size used in the data comparison should be noted (n = 9 for δ34S, compared to n = 103 for δ13C and δ15N). Reindeer δ34S values reflect the soil δ34S values upon which they fed. Soil δ34S values are related to underlying lithology and can be altered through changing rates of mineral weather and soil-bedrock interactions, controlled by hydrological conditions and bacterially-mediated reduction and oxidation processes51,54,55. The surface lithology of the regions compared (southwest France, northern Swiss Jura, and French Jura) are underlain by similar late Mesozoic and Cenozoic limestone and sandstone deposits72, which are unlikely to vary in δ34S values to the extent observed in the reindeer data. This suggests environmental parameters are influential in the observed signal. Low environmental δ34S values have been related to low oxygen/water-logged soil conditions, though the processes that govern these fractionations are complex and not yet fully understood55,73,74,75. It is therefore possible that the lower δ34S values observed in the Swiss samples, compared to the French samples, could indicate an environment that had experienced wetter/more oxygen limited soil conditions. Such conditions could have been brought about by changing Alpine precipitation regimes, as identified in local speleothem records2, or by localised permafrost thaw related to the (albeit small) increased temperatures of GI-2.
Conclusion
The refined chronological model presented here, based on current available archaeological evidence, indicates that the LGM human occupation of Switzerland was likely restricted to a brief period of local climatic warming, most probably associated with Greenland Interstadial 2. This supports the hypothesis put forward by Terberger and Street6. Stable isotope analysis of reindeer bone collagen, directly associated with human activity, further supports this interpretation. While the data indicates the landscapes in which humans hunted reindeer remained comparatively cold, it also suggests relatively stable environmental conditions. Compared to other European reindeer data, the higher carbon isotope values suggest a greater reliance on lichen by the Swiss reindeer, indicating that other vegetation types may have been more limited. The low sulphur and intermediary nitrogen isotope values could represent the influence of reduced/changing soil bacterial activity related to a brief respite in the most severe climatic conditions associated with the global LGM and local glacial advance. Combined, this data supports the hypothesis that human activity in Switzerland was made possible by a phase of climatic warming that occurred during the part of MIS2 that is more broadly characterised by global glacial advance.
While the chronologies of many of the central European LGM archaeological sites are currently too imprecise to permit robust comparison, the striking similarities between the revised chronology for Switzerland and that recently published for Kammern-Grubgraben in Austria23, offer a tantalising insight into what could be a relatively brief episode of LGM human activity across central Europe, facilitated by the warmer environmental conditions that GI-2 provided. Whether the other known LGM archaeological sites in central Europe also correspond to this warmer period, or whether they represent different episodes of human activity during the LGM, can only be further examined through better chronological characterisation of these sites. Additionally, we stress the importance of also obtaining location-specific palaeoenvironmental records when making inferences about the types of landscapes in which human occupation took place.
Methods
0.2 to 1.3 g of bone sample was collected from each specimen using a dental drill. Collagen extraction was performed at University College London (UCL) using a modified version of the Oxford Radiocarbon Accelerator Unit (ORAU) collagen extraction procedure (AF)31, which is based on a modified version of the Longin75 method. All samples were treated with 0.5 M hydrochloric acid (HCl) at 4 °C until fully demineralised (24hrs – 2 weeks), then thoroughly rinsed with ultrapure water. Demineralised samples were then gelatinised in pH3 HCl solution at 75 °C for 48hrs and filtered using a pre-cleaned Ezee-filter. The filtrate was passed through a pre-cleaned 15–30 kD ultrafilter, with the >30 kD fraction collected and lyophilised.
For stable isotope analysis, 1.2–1.5 mg aliquots of purified collagen were weighed into tin capsules and analysed using a Delta V Advantage continuous-flow isotope ratio mass spectrometer coupled via a ConfloIV to an EA IsoLink elemental analyser (Thermo Fisher Scientific, Bremen) at the Scottish Universities Environmental Research Centre (SUERC). For every ten unknown samples, three in-house standards that are calibrated to the International Atomic Energy Agency (IAEA) reference materials USGS40 (L-glutamic acid, δ13CVPDB = –26.4‰, δ15NAIR = –4.5‰), USGS41 (L-glutamic acid, δ13CVPDB = +37.6‰, δ15NAIR = –47.6‰), USGS43 (Indian Human Hair: δ15NAIR = +8.44‰, δ13CVPDB = –21.28‰, δ34SVCDT = +10.46‰), IAEA-S-2 (silver sulfide, δ34SVCTD = +22.7‰), and IAEA-S-3 (silver sulfide, δ34SVCTD = –32.3‰) were analysed76. Results are reported as per mil (‰) relative to the internationally accepted standards VPDB, AIR and VCDT. Measurement uncertainty was determined to be ±0.1‰ for δ13C, ±0.2‰ for δ15N, and ±0.3‰ for δ34S on the basis of repeated measurements of an in-house bone collagen standard and a certified fish gelatin standard (Elemental Microanalysis, UK).
Radiocarbon dating was performed at ORAU using their standard procedures, as described by Brock et al.31. Approximately 5 mg of purified collagen was weighed into a tin capsule that had been baked at 500 °C for 12 hours. Samples were combusted using an elemental analyser coupled to an isotope ratio mass spectrometer, employing a splitter to allow for collection of the CO2 31,77. Samples were graphitised by reduction of collected CO2 over an iron catalyst in an excess H2 atmosphere at 560 °C78,79. 14C dates were measured on the Oxford AMS system using a caesium ion source for ionisation of the solid graphite sample24. To denote the bone pre-treatment at UCL rather than at ORAU, all measured dates were given “OxA-V-wwww-pp” numbers, where “wwww” indicates the wheel number, and “pp” is the position of the sample on the wheel24. As bone pre-treatment was performed in the laboratory at UCL dates were corrected for potential background contamination within this laboratory using two known-age reference samples80. Corrected dates are denoted by adding a “C” to the end of the date code assigned by ORAU. Uncorrected measured date values as well as further details of the correction calculations are provided in Supplemental Information 3. Details of the pre-treatment procedure used in the previous dating of these samples is given in Supplementary Information 4.
Data availability
All data is provided in the supplementary information.
References
Preusser, F., Graf, H. R., Keller, O., Krayss, E. & Schlüchter, C. Quaternary glaciation history of northern Switzerland. EG Quaternary Sci. 60, 282–305 (2011).
Luetscher, M. et al. North Atlantic storm track changes during the Last Glacial Maximum recorded by Alpine speleothems. Nat. Commun. 6, 6344, https://doi.org/10.1038/ncomms7344 (2015).
Bini, A. et al. La Suisse durant le denier maximum glaciaire (LGM) 1:500000. (Federal Office of Topography Swisstopo, Switzerland, 2009).
Schweizer, T., Schmid, E. & Bay, R. Die «Kastelhöhle» im Kalt-brunnental, Gemeinde Himmelried (Solothurn). Jahrbuch für Solothurnische Geschichte 32, 1–88 (1959).
Sedlmeier, J. Die jungpaläolithischen Funde aus der Mittleren Fundschicht der Kastelhöhle-Nord im nordwestschweizerischen Kaltbrunnental, Himmelried SO. Ein Nachweis für die Begehung der Schweiz durch Mensch und Tier kurz nach dem Kältemaximum der letzten Eiszeit. Jahrb. der Archäologie Schweiz. 93, 7–34 (2010).
Terberger, T. & Street, M. Hiatus or continuity? New results for the question of pleniglacial settlement in Central Europe. Antiquity 76, 691–698 (2002).
Strandberg, G., Brandefelt, J., Kjellstrom, E. & Smith, B. High resolution regional simulation of last glacial maximum climate in Europe. Tellus 63, 107–125 (2011).
Heyman, B. M., Heyman, J., Fickert, T. & Harbor, J. M. Paleoclimate of the central European uplands during the last glacial maximum based on glacier mass-balance modelling. Quaternary Res. 79, 49–54 (2013).
Rasmussen, S. O. et al. A stratigraphic framework for abrupt climatic changes during the Last Glacial period based on three synchronized Greenland ice-core records: refining and extending the INTIMATE event stratigraphy. Quaternary Sci. Rev. 106, 14–28 (2014).
Hughes, P. D., Gibbard, P. L. & Ehlers, J. Timing of glaciation during the last glacial cycle: evaluating the concept of a global ‘Last Glacial Maximum’ (LGM). Earth-Sci Rev. 125, 171–198 (2013).
Hughes, P. D. & Gibbard, P. L. A stratigraphical basis for the Last Glacial Maximum (LGM). Quatern Int. 383, 174–185 (2015).
Ivy-Ochs, S., Schäfer, J., Kubik, P. W., Synal, H.-A. & Schlüchter, C. The timing of deglaciation on the northern Alpine foreland (Switzerland). Eclogae Geol. Helv. 97, 47–55 (2004).
Reber, R. et al. Timing of retreat of the Reuss Glacier (Switzerland) at the end of the Last Glacial Maximum. Swiss J. Geosci. 107, 293–307 (2014).
Haeberli, W. Glaciological conditions in Northern Switzerland during recent Ice Ages. (Nationale Genossenschaft für die Lagerung radioaktiver Abfälle, Wettingen, CH, 2010).
Binney, H. et al. Vegetation of Eurasia from the last glacial maximum to present: Key biogeographic patterns. Quaternary Sci. Rev. 157, 80–97 (2017).
Scapozza, C., Castelletti, C., Soma, L., Dall’Agnolo, S. & Ambrosi, C. Timing of LGM and deglaciation in the southern Swiss Alps. Géomorphologie 4, 307–322 (2015).
Ivy‐Ochs, S. Glacier variations in the European Alps at the end of the last glaciation. Cuad. Invest. Geogr. 41, 295–315 (2015).
Monegato, G. et al. Evidence of a two-fold glacial advance during the last glacial maximum in the Tagliamento end moraine system (eastern Alps). Quat. Res. 68, 284–302 (2007).
Street, M. & Terberger, T. The last Pleniglacial and the human settlement-of Central Europe: new information from the Rhineland site of Wiesbaden-Igstadt. Antiquity 73, 259–272 (1999).
Svoboda, J., Lozek, V. & Vlcek, E. Hunters between east and west: the Paleolithic of Moravia. (Plenum Press, London, 1996).
Verpoorte, A. Eastern Central Europe during the Pleniglacial. Antiquity 78, 257–266 (2004).
Salcher-Jedrasiak, T. A., Nývltová Fišáková, M. & Fladerer, F. A. New warm season data from the 21 ka BP mammoth hunters’ camp site at Langmannersdorf an der Perschling (Lower Austria). Poster presented at International Council for Zooarchaeology (ICAZ) conference, Paris. http://alexandriaarchive.org/bonecommons/exhibits/show/icaz2010paris/session4_3/item/1511 (2010).
Haesaerts, P. & Damblon, F. Radiocarbon chronology of the Late Palaeolithic loess site of Kammern-Grubgraben (Lower Austria). Archaeologia Austriaca 100, 271–277 (2016).
Bronk Ramsey, C., Higham, T., Bowles, A. & Hedges, R. E. M. Improvements to the pretreatment of bone at Oxford. Radiocarb. 46, 155–63 (2004).
Higham, T. G., Jacobi, R. M. & Bronk Ramsey, C. AMS radiocarbon dating of ancient bone using ultrafiltration. Radiocarb. 48, 179–195 (2006).
Fülöp, R. H., Rethmeyer, J., Heinze, S. & König, S. Ultrafiltration is neither the problem nor the solution. Radiocarb. 55, 491–500 (2013).
Huels, M., der Plicht, V., Brock, J., Matzerath, F. & Chivall, S. D. Laboratory intercomparison of Pleistocene bone radiocarbon dating protocols. Radiocarb. 59, 1543–1552 (2017).
Hüls, C. M., Grootes, P. M. & Nadeau, M.-J. Ultrafiltration: boon or bane? Radiocarb. 51, 613–25 (2009).
Minami, M., Yamazaki, K., Omori, T. & Nakamura, T. Radiocarbon dating of VIRI bone samples using ultrafiltration. Nucl. Instrum. Methods B 294, 240–245 (2013).
Brock, F., Bronk Ramsey, C. & Higham, T. F. G. Quality assurance of ultrafiltered bone dating. Radiocarb. 49, 187–192 (2007).
Brock, F., Higham, T. F. G., Ditchfield, P. & Bronk Ramsey, C. Current pretreatment methods for AMS radiocarbon dating at the Oxford Radiocarbon Accelerator Unit (ORAU). Radiocarb. 52, 103–112 (2010).
Jacobi, R. M., Higham, T. F. G. & Bronk Ramsey, C. AMS radiocarbon dating of Middle and Upper Palaeolithic bone in the British Isles: improved reliability using ultrafiltration. J. Quaternary Sci. 21, 557–573 (2006).
Jacobi, R. M. & Higham, T. F. G. The early Lateglacial re-colonization of Britain: new radiocarbon evidence from Gough’s Cave, southwest England. Quaternary Sci. Rev. 28, 1895–1913 (2009).
Higham, T. F. G. et al. Precision dating of the Palaeolithic: a new radiocarbon chronology for the Abri Pataud (France), a key Aurignacian sequence. J. Hum. Evol. 61, 549–563 (2011).
Stevens, R. E. & Hedges, R. E. M. Carbon and nitrogen stable isotope analysis of northwest European horse bone and tooth collagen, 40,000 BP-present: palaeoclimatic interpretations. Quaternary Sci. Rev. 23, 977–991 (2004).
Stevens, R. E. et al. Nitrogen isotope analyses of reindeer (Rangifer tarandus), 45,000 BP to 9,000 BP: Palaeoenvironmental reconstructions. Palaeogeogr. Palaeoclimatol. Palaeoecol. 262, 32–45 (2008).
Drucker, D. G., Kind, C. J. & Stephan, E. Chronological and ecological information on Late-glacial and early Holocene reindeer from northwest Europe using radiocarbon (14C) and stable isotope (13C, 15N) analysis of bone collagen: case study in southwestern Germany. Quatern Int. 245, 218–224 (2011).
Drucker, D. G., Bridault, A. & Cupillard, C. Environmental context of the Magdalenian settlement in the Jura Mountains using stable isotope tracking (13C, 15N, 34S) of bone collagen from reindeer (Rangifer tarandus). Quatern Int. 272-273, 268–278 (2012).
Stevens, R. E., Hermoso-Buxán, X. L., Marín-Arroyo, A. B., González-Morales, M. R. & Straus, L. G. Investigation of Late Pleistocene and Early Holocene palaeoenvironmental change at El Mirón cave (Cantabria, Spain): insights from carbon and nitrogen isotope analyses of red deer. Palaeogeogr. Palaeoclimatol. Palaeoecol. 414, 46–60 (2014).
Drucker, D. G. et al. Environment and subsistence in north-western Europe during the Younger Dryas: An isotopic study of the human of Rhünda (Germany). J. Archaeol. Sci. Rep. 6, 690–699 (2016).
Drucker, D. G., Stevens, R. E., Germonpré, M., Sablin, M. V. & Bocherens, H. Collagen stable isotopes provide insights into the end of the mammoth steppe in the central East European plains during the Epigravettian. Quaternary Res. 90, 457–469 (2018).
Drucker, D. G., Hobson, K. A., Ouellet, J.-P. & Courtois, R. Influence of forage preferences and habitat use on 13C and 15N abundance in wild caribou (Rangifer tarandus caribou) and moose (Alces alces) from Canada. Isot. Env. Healt S 46, 107–121 (2010).
Ben-David, M., Shochat, E. & Adams, L. G. The utility of stable isotope analysis in studying the foraging ecology of herbivores: Examples from moose and caribou. Alces 37, 421–434 (2001).
Heaton, T. H. E. Spatial, species, and temporal variations in the13C/12C ratios of C3 plants: implications for palaeodiet studies. J. Archaeol. Sci. 26, 637–649 (1999).
Drucker, D. G., Bridault, A., Hobson, K. A., Szuma, E. & Bocherens, H. Can carbon-13 in large herbivores reflect the canopy effect in temperate and boreal ecosystems? Evidence from modern and ancient ungulates. Palaeogeogr. Palaeoclimatol. Palaeoecol. 266, 69–82 (2008).
Kohn, M. J. Carbon isotope compositions of terrestrial C3 plants as indicators of (paleo)ecology and (paleo)climate. P Natl Acad. Sci. USA 107, 19691–19695 (2010).
Fox-Dobbs, K., Leonard, J. A. & Koch, P. L. Pleistocene megafauna from eastern Beringia: Paleoecological and paleoenvironmental. interpretations of stable carbon and nitrogen isotope and radiocarbon records. Palaeogeogr. Palaeoclimatol. Palaeoecol. 261, 30–46 (2008).
Bocherens, H. et al. Reconstruction of the Gravettian food-web at Předmostí I using multi-isotopic tracking (13C, 15N, 34S) of bone collagen. Quatern Int. 359-360, 211–228 (2015).
Amundson, R. et al. Global patterns of the isotopic composition of soil and plant nitrogen. Glob. Biogeochem. Cy 17, 1031 (2003).
Craine, J. M. et al. Convergence of soil nitrogen isotopes across global climate gradients. Sci. Rep. 5, 8280 (2015).
Nehlich, O. The application of sulphur isotope analyses in archaeological research: A review. Earth-Sci Rev. 142, 1–17 (2015).
Jones, J. R., Richards, M. P., Reade, H., de Quiros, F. B. & Marin-Arroyo, A. B. Multi-Isotope investigations of ungulate bones and teeth from El Castillo and Covalejos caves (Cantabria, Spain): Implications for paleoenvironment reconstructions across the Middle-Upper Palaeolithic transition. J. Archaeol. Sci. Rep. 23, 1029–1042 (2018).
Wißing, C. et al. Stable isotopes reveal patterns of diet and mobility in the last Neandertals and first modern humans in Europe. Sci. Rep. 9, 4433 (2019).
Thode, H.G. Sulfur isotopes in nature and the environment: an overview in Stable Isotopes: Natural and Anthropogenic Sulfur in the Environment 1 (eds. Krouse, H.R. & Grinenko, V.A.), 1–26 (John Wiley & Sons, 1991).
Nitsch, E. K. et al. The Preservation and Interpretation of δ34S Values in Charred Archaeobotanical Remains. Archaeometry 61, 161–178 (2019).
Sealy, J., Johnson, M., Richards, M. & Nehlich, O. Comparison of two methods of extracting bone collagen for stable carbon and nitrogen isotope analysis: comparing whole bone demineralization with gelatinization and ultrafiltration. J. Archaeol. Sci. 47, 64–69 (2014).
DeNiro, M. J. Postmortem preservation and alteration of in vivo bone collagen isotope ratios in relation to palaeodietary reconstruction. Nat. 317, 806–809 (1985).
Ambrose, S. H. Preparation and characterization of bone and tooth collagen for isotopic analysis. J. Archaeol. Sci. 17, 431–451 (1990).
Nehlich, O. & Richards, M. P. Establishing quality criteria for sulphur isotope analysis of archaeological bone collagen. Archaeol. Anthr. Sci. 1, 59–75 (2009).
Bronk Ramsey, C., Higham, T. F. G., Brock, F., Baker, D. & Ditchfield, P. Radiocarbon dates from the Oxford AMS system: archaeometry datelist 33. Radiocarb. 51, 323–349 (2009).
Brooks, J. R., Flanagan, L. B., Buchman, N. & Ehleringer, J. R. Carbon isotope composition of boreal plants: functional grouping of life forms. Oecologia 110, 301–311 (1997).
Holleman, D. F., Luick, J. R. & White, R. G. Lichen intake estimates for reindeer and caribou during winter. J. Wildl. Manage. 43, 92–201 (1979).
Fancy, S. G., Pank, L. F., Whitten, K. R. & Regelin, W. L. Seasonal movements of caribou in arctic Alaska as determined by satellite. Can. J. Zoo. 67, 644–650 (1989).
Ferguson, S. H. & Elkie, P. C. Seasonal movement patterns of woodland caribou. J. Zool. 262, 125–134 (2004).
Tyler, N. J. C. & Øritsland, N. A. Why don’t Svalbard reindeer migrate? Holarct. Ecol. 12, 369–376 (1989).
Weinstock, J. Late Paleolithic reindeer populations in central and western. Europe. Anthropozoologica 25-26, 383–388 (1997).
Britton, K. et al. Strontium isotope evidence for migration in late Pleistocene Rangifer: Implications for Neanderthal hunting strategies at the Middle Palaeolithic site of Jonzac, France. J. Hum. Evol. 61, 176–185 (2011).
Fontana, L. The four seasons of reindeer: Non-migrating reindeer in the Dordogne region (France) between 30 and 18k? Data from the Middle and Upper Magdalenian at La Madeleine and methods of seasonality determination. J. Archaeol. Sci. Rep. 12, 346–362 (2017).
Price, D. T., Meiggs, D., Weber, M.-J. & Pike-Tay, A. The migration of Late Pleistocene reindeer: isotopic evidence from northern Europe. Archaeol. Anthr. Sci. 9, 371–394 (2017).
Drucker, D. G. et al. Tracking possible decline of woolly mammoth during the Gravettian in Dordogne (France) and the Ach Valley (Germany) using multi-isotope tracking (13C, 14C, 15N, 34S, 18O). Quaternary Int. 359-360, 304–317 (2015).
Körner, C., Farquhar, G.D. & Wong, S.C. Carbon isotope discrimination by plants follows latitudinal and altitudinal trends. Oecologia 88, 30–40.
EGDI: European Geological Data Infrastructure. 1:1 Million OneGeology pan-European Surface Geology. Version: 5.020, http://www.europe-geology.eu/ (2019)
Fry, B., Scalan, R. S., Winters, J. K. & Parker, P. L. Sulphur uptake by salt grasses, mangroves, and seagrasses in anaerobic sediments. Geochim. Cosmochim. Ac. 46, 1121–1124 (1982).
Groscheová, H., Novák, M. & Alewell, C. Changes in the δ34S ratio of pore-water sulfate in incubated Sphagnum peat. Wetl. 20, 62–69 (2000).
Longin, R. New method of collagen extraction for radiocarbon dating. Nat. 230, 241–242 (1971).
Sayle, K. L., Brodie, C. R., Cook, G. T. & Hamilton, W. D. Sequential measurement of δ15N, δ13C and δ34S values in archaeological bone collagen at the Scottish Universities Environmental Research centre (SUERC): A new analytical frontier. Rapid Commun. Mass. Spectrom. 33, 1258–1266 (2019).
Bronk Ramsey, C. & Humm, M. J. On-line combustion of samples for AMS and ion source developmets at ORAU. Nucl. Instr. Meth Phys. Res. B. 172, 242–246 (2000).
Bronk Ramsey, C. & Hedges, R. E. M. Hybrid ion sources: Radiocarbon measurements from microgram to milligram. Nucl. Instr. Meth Phys. Res. B 123, 539–45 (1997).
Dee, M. & Bronk Ramsey, C. Refinement of graphite target production at ORAU. Nucl. Instr. Meth Phys. Res. B. 172, 449–453 (2000).
Wood, R. E., Bronk Ramsey, C. & Higham, T. G. Refining background corrections for radiocarbon dating of bone collagen at ORAU. Radiocarb. 52, 600–611 (2010).
Geologische Bundesanstalt. Der Alpenraum zum Höhepunkt der letzten Eiszeit: Rekonstruktion der maximalen Gletscherausbreitung während des Höhepunktes der letzten Eiszeit (Würm) von 26 000 bis 20 000 Jahren vor heute. (Verlag der Geologischen Bundesanstalt (GBA), Vienna, 2013).
Bronk Ramsey, C. Methods for Summarizing Radiocarbon Datasets. Radiocarb. 59, 1809–1833 (2017).
Reimer, P. J. et al. IntCal13 and Marine13 Radiocarbon Age Calibration Curves 0-50,000 Years cal BP. Radiocarb. 55, 1869–1887 (2013).
Andersen, K. K. et al. The Greenland Ice Core Chronology 2005, 15-42 ka. Part 1: Constructing the time scale. Quaternary Sci. Rev. 25, 3246–3257 (2006).
Svensson, A. et al. The Greenland Ice Core Chronology 2005, 15-42 ka. Part 2: Comparison to other records. Quaternary Sci. Rev. 25, 3258–3267 (2006).
Drucker, D. G., Bocherens, H. & Billiou, D. Evidence for shifting environmental conditions in Southwestern France from 33 000 to 15 000 years ago derived from carbon-13 and nitrogen-15 natural abundances in collagen of large herbivores. Earth Planet. Sc. Lett. 216, 163–173 (2003).
Vercoutère, C. et al. Deer (Rangifer tarandus and Cervus elaphus) remains from the Final Gravettian of the Abri Pataud and their importance to humans in Deer and People (eds. Baker, K., Carden, R. & Madgwick, R.), 145–158 (Oxbow Books, 2014).
Drucker, D. G., Bocherens, H., Cleyet-Merie, J.-J., Madelaine, S. & Mariotti, A. Implications paléoenvironnementales de l'étude isotopique (¹³C, 15N) de la faune des grands mammifères des Jamblancs (Dordogne, France). Paléo 12, 127–140 (2000).
Acknowledgements
We thank Denise Leesch, Werner Müller, and the Archaeological Services of the cantons of Soluthurn and Neuchâtel for providing access to the material included in this research. We are grateful to the Mary Rose Trust for their donation of cow ribs from the Mary Rose shipwreck, which provided known-age standard material for radiocarbon analysis. Emily Walsh is thanked for her assistance with the collagen extraction laboratory procedure. This research was funded by a European Research Council Consolidator Grant awarded to RES (grant ID: 617777).
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R.E.S., H.R., S.G., T.H. and I.B. contributed to the conception and design of the project. H.R. and S.C. conducted the sampling. H.R., J.T., A.F. and K.L.S. undertook laboratory processing and analysis of the samples. H.R., J.T. and R.E.S. interpreted the data. H.R. wrote the initial draft of the manuscript. All authors reviewed and edited the manuscript. R.E.S. secured the funding for the research.
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Reade, H., Tripp, J.A., Charlton, S. et al. Radiocarbon chronology and environmental context of Last Glacial Maximum human occupation in Switzerland. Sci Rep 10, 4694 (2020). https://doi.org/10.1038/s41598-020-61448-7
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DOI: https://doi.org/10.1038/s41598-020-61448-7
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