B cells can promote protective immunity to viruses and this has been attributed to their production of high-affinity neutralizing antibodies. A new study by the von Andrian group has challenged this view; it indicates that the ability of B cells to support innate immune responses might be more important than their adaptive immune functions during infection of mice with vesicular stomatitis virus (VSV).
B cells have antibody-independent protective functions during viral infection
Using a subcutaneous infection protocol — which mimics the natural route of VSV transmission — the authors recently showed that subcapsular sinus (SCS) macrophages in the draining lymph nodes are essential for protective immunity to VSV (see further reading). Productive infection of SCS macrophages and replication of VSV promotes early production of type I interferons (IFNs) and prevents VSV from spreading to the central nervous system. In this study, the authors were interested in defining the role of B cells during VSV infection. They used two transgenic mouse strains: μMT mice, which lack all B cells and have abnormal lymphoid tissue architecture; and DHLMP2A mice, in which B cells do not express cell-surface B cell receptors and cannot secrete antibodies, but still support normal lymphoid tissue development. Surprisingly, whereas 60% of μMT mice had fatal neuroinvasion during subcutaneous VSV infection, all of the DHLMP2A mice were protected. This indicates that B cells have antibody-independent protective functions during viral infection.
Exploring this further, the authors found that B cells are required to maintain distinct populations of medullary and SCS macrophages in lymph nodes. The lymph nodes of μMT mice contained fewer total macrophages, and ∼90% of these macrophages had a medullary phenotype. By contrast, in wild-type or DHLMP2A mice, only ∼40% of lymph node macrophages had a medullary phenotype, whereas ∼60% had an SCS phenotype. Macrophages from μMT mice could capture and retain lymph-borne VSV in a similar manner to wild-type controls. However, VSV failed to replicate in the μMT macrophages and these cells could not generate a protective IFN response. The ability of B cells to maintain functional lymph node macrophages with a virus-protective SCS phenotype depended on their production of lymphotoxin-α1β2 (LTα1β2). When LTα1β2-mediated signalling was blocked in wild-type mice, lymph node macrophages showed aberrant differentiation and were not permissive to VSV replication and IFN induction.
Finally, to assess the contribution of adaptive immune mechanisms other than antibody production, the authors depleted T cells from DHLMP2A mice before subcutaneous VSV infection. Notably, the mice did not have increased susceptibility to VSV and all survived the infection. These findings indicate that innate immune mechanisms are sufficient to protect the host during VSV infection.
References
ORIGINAL RESEARCH PAPER
Moseman, E. A. et al. B cell maintenance of subcapsular sinus macrophages protects against a fatal viral infection independent of adaptive immunity. Immunity 1 Mar 2012 (doi:10.1016/j.immuni.2012.01.013)
FURTHER READING
Leavy, O. Antiviral immunity: SCS macrophages protect the CNS. Nature Rev. Immunol. 8, 538 (2010)Article
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Bordon, Y. An innate talent uncovered. Nat Rev Immunol 12, 229 (2012). https://doi.org/10.1038/nri3200
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DOI: https://doi.org/10.1038/nri3200