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The expression of P2Y14, a purinergic G-protein coupled receptor, defines functionally distinct subpopulations in placenta-derived hematopoietic stem progenitor cells

Leukemia volume 31, pages 2837–2841 (2017)Cite this article

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Hematopoietic stem cell transplantation (HSCT) is, so far, the only everlasting treatment for leukemia. Cancer patients who go through high-dose chemotherapy or radiation therapy also require HSCT. One of the major obstacles in achieving a successful stem cell transplant is an insufficient number of hematopoietic stem progenitor cells (HSPC), which are present at very low frequencies in hematopoietic tissues, such as the bone marrow, blood and spleen.

Induced pluripotent stem cells (iPSC) hold potential promise for the generation of any cell type of interest due to their pluripotent nature, and considerable efforts have been made to derive HSPCs from iPSC cells.1, 2, 3 Additionally, there have been various attempts to expand HSPCs in vivo and in vitro.4 Despite these endeavors, it still remains questionable as to whether iPSC-derived or in vitro-expanded HSPCs maintain the hallmarks of stem cells that include self-renewal, multipotentiality and homing properties. It is also necessary to scrutinize whether manipulated HSPCs, when infused in humans, increase risk of leukemia or other cancers on a long term basis.

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References

  1. Alvarez-Silva M, Belo-Diabangouaya P, Salaun J, Dieterlen-Lievre F . Mouse placenta is a major hematopoietic organ. Development 2003; 130: 5437–5444.

    Article  CAS  PubMed  Google Scholar 

  2. Amabile G, Welner RS, Nombela-Arrieta C, D'Alise AM, Di Ruscio A, Ebralidze AK et al. In vivo generation of transplantable human hematopoietic cells from induced pluripotent stem cells. Blood 2013; 121: 1255–1264.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Hanna J, Wernig M, Markoulaki S, Sun CW, Meissner A, Cassady JP et al. Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin. Science 2007; 318: 1920–1923.

    Article  CAS  PubMed  Google Scholar 

  4. Sauvageau G, Iscove NN, Humphries RK . In vitro and in vivo expansion of hematopoietic stem cells. Oncogene 2004; 23: 7223–7232.

    Article  CAS  Google Scholar 

  5. Cairo MS, Wagner JE . Placental and/or umbilical cord blood: an alternative source of hematopoietic stem cells for transplantation. Blood 1997; 90: 4665–4678.

    CAS  PubMed  Google Scholar 

  6. Gekas C, Rhodes KE, Mikkola HK . Isolation and visualization of mouse placental hematopoietic stem cells. Curr Protoc Stem Cell Biol 2008; Chapter 2: Unit 2A 8 1–2A 8 14.

    Article  Google Scholar 

  7. Ottersbach K, Dzierzak E . The murine placenta contains hematopoietic stem cells within the vascular labyrinth region. Dev Cell 2005; 8: 377–387.

    Article  CAS  PubMed  Google Scholar 

  8. Robin C, Bollerot K, Mendes S, Haak E, Crisan M, Cerisoli F et al. Human placenta is a potent hematopoietic niche containing hematopoietic stem and progenitor cells throughout development. Cell Stem Cell 2009; 5: 385–395.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Cho J, Yusuf R, Kook S, Attar E, Lee D, Park B et al. Purinergic P2Y14 receptor modulates stress-induced hematopoietic stem/progenitor cell senescence. J Clin Invest 2014; 124: 3159–3171.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Kook S, Cho J, Lee SB, Lee BC . The nucleotide sugar UDP-glucose mobilizes long-term repopulating primitive hematopoietic cells. J Clin Invest 2013; 123: 3420–3435.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Lee BC, Cheng T, Adams GB, Attar EC, Miura N, Lee SB et al. P2Y-like receptor, GPR105 (P2Y14), identifies and mediates chemotaxis of bone-marrow hematopoietic stem cells. Genes Dev 2003; 17: 1592–1604.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Chadet S, Jelassi B, Wannous R, Angoulvant D, Chevalier S, Besson P et al. The activation of P2Y2 receptors increases MCF-7 breast cancer cells migration through the MEK-ERK1/2 signalling pathway. Carcinogenesis 2014; 35: 1238–1247.

    Article  CAS  PubMed  Google Scholar 

  13. Williams DA, Xu H, Cancelas JA . Children are not little adults: just ask their hematopoietic stem cells. J Clin Invest 2006; 116: 2593–2596.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Ditadi A, de Coppi P, Picone O, Gautreau L, Smati R, Six E et al. Human and murine amniotic fluid c-Kit+Lin- cells display hematopoietic activity. Blood 2009; 113: 3953–3960.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors are grateful to Min-Hye Kim for animal management for the use of competitor and recipient mice. This research was supported in part by the Department of Defense (W81XWH-09-1-0364 to BCL); and by the National Basic Research Program (2011CB964800) and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (2017R1A2B4009269 and 2016R1A2A2A05921669) and the Ministry of Education (2017R1A6A3A11028201).

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  1. S-H Kook and H-J Sim: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Bioactive Material Sciences, Research Center of Bioactive Materials, Chonbuk National University, Jeonju, South Korea

    S-H Kook, H-J Sim & J-C Lee

  2. Department of Medicine, Division of Hematology and Oncology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    S-H Kook & B-C Lee

  3. Cluster for Craniofacial Development & Regeneration Research, Institute of Oral Biosciences, Chonbuk National University, Jeonju, South Korea

    H-J Sim & J-C Lee

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  1. S-H Kook
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Correspondence to S-H Kook or B-C Lee.

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Kook, SH., Sim, HJ., Lee, JC. et al. The expression of P2Y14, a purinergic G-protein coupled receptor, defines functionally distinct subpopulations in placenta-derived hematopoietic stem progenitor cells. Leukemia 31, 2837–2841 (2017). https://doi.org/10.1038/leu.2017.254

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