Abstract
The alarming rise of hospital- and community-associated methicillin-resistant Staphylococcus aureus (HA- and CA-MRSA) infections has prompted a desperate search for novel antibiotics. We discovered the streptogramin etamycin produced by an actinomycete species isolated from the coast of Fiji, the first time this antibiotic has been identified from a marine microbe. Etamycin was extracted and purified from this strain (CNS-575) and identified as a three-rotamer species by 2D NMR spectroscopy. Etamycin demonstrated potent activity against HA- and CA-MRSA in microbroth dilution assays, with minimum inhibitory concentrations (MIC) as low as 1â2âmgâlâ1 against HA- and CA-MRSA strains. Furthermore, etamycin was also active against other Gram-positive and several Gram-negative pathogens and was found to be non-cytotoxic at concentrations more than 20-fold above MIC. Etamycin displayed favorable time-kill kinetics compared with the first-line MRSA antibiotic, vancomycin, and also conferred significant protection from mortality in a murine model of systemic lethal MRSA infection. These data emphasize the utility of the marine environment as a relatively untapped source of antibiotics against major drug-resistant human pathogens. These studies will also guide future isolation and preclinical development of depsipeptide anti-MRSA compounds from marine-derived actinomycetes.
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Introduction
The alarming rise of methicillin-resistant Staphylococcus aureus (MRSA) infections in several countries worldwide has provided urgency to the search for structurally novel antibiotics that target these organisms.1 Hospital-associated (HA)-MRSA are frequently resistant to multiple antibiotic classes, and rapidly emergent strains of community-associated (CA)-MRSA, which have demonstrated unusually high virulence and ease of transmission, now represent the most common cause of skin and soft tissue infections in the United States.2, 3 Furthermore, the approval of novel antibiotics for human medicine has not kept pace with the development of resistance by important bacterial pathogens such as MRSA. Just a few drugs with novel scaffolds (linezolid, daptomycin) have been introduced to the antibacterial formulary in the past 11 years, and only three new antibacterial compounds are in advanced stages of clinical development.4, 5 Both HA- and CA-MRSA strains have already demonstrated the capacity to evolve resistance to linezolid and daptomycin.
Natural products represent a vast source of potential new chemical structures possessing antimicrobial activity. At least three of every four current antibacterial agents are related in some way to natural products,6 and a majority of these discoveries have come from filamentous bacteria of the order Actinomycetales (actinomycetes). More than 9000 biologically active molecules have been isolated from actinomycetes, yielding >60 pharmaceutical agents used in the medical or agricultural fields.6 Although terrestrial (soil) actinomycetes were the mainstay for antibiotic discovery efforts in the previous five decades, there is evidence that this resource is becoming exhausted. In contrast, the marine environment, covering more than three-fourths of the world's surface, represents a vast and relatively untapped source of novel scaffolds with unique antimicrobial properties.7, 8, 9, 10 Recently, structures identified from new marine actinomycete species have been shown to exhibit potent anti-MRSA activity with desirable cross-activity against other bacterial pathogens.11, 12
In the course of screening a library of extracts from previously unidentified species of marine-derived actinomycetes for anti-MRSA activity, we followed a hit and purified an active secondary metabolite that we confirmed by NMR and mass spectrometric analysis to be etamycinâa classical depsipeptide antibiotic of the streptogramin B class. As decades have elapsed since consideration of the antibiotic properties of etamycin, we undertook an evaluation of its activity against a panel of contemporary, clinically relevant HA- and CA-MRSA strains. We find that etamycin exhibits favorable in vitro antibacterial activity and killing kinetics and is also protective against MRSA-induced lethality in a murine systemic infection model. These data suggest that etamycin could serve as the basis for in-depth study of related marine-derived molecules as MRSA-targeted therapies.
Materials and methods
Bacterial strains
A panel of HA- and CA-MRSA and other Gram-positive and -negative bacterial strains was used to probe the antimicrobial activity of etamycin. These included MRSA strains ATCC 33591, NRS22 (USA600), NRS71 (Sanger 252, genome strain), NRS119, USA200 (UAMS-1), NRS192, USA300 (UAMS-1182) and strain NRS386 (USA700). Isolates with the NRS designation were obtained through the Network of Antimicrobial Resistance in Staphylococcus aureus (NARSA) program supported under NIAID/NIH contract # HHSN272200700055C. The USA200 and USA300 isolates were kind gifts of Greg Somerville at the University of Nebraska, Lincoln, and originally obtained from Mark Smeltzer at the University of Arkansas Medical Center. Other Gram-positive strains tested were Streptococcus pyogenes serotype M1 strain 5448,13 Streptococcus agalactiae strain COH114 and vancomycin-resistant Enterococcus faecalis (ATCC 51299). The Gram-negative strains used were Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Moraxella catarrhalis (ATCC 25238) and Haemophilus influenzae (Type b, ATCC 10211).
The actinomycete strain CNS-575 was isolated from a sediment sample collected from the Nasese shoreline, Viti Levu, Fiji, at low tide from a depth of â0.5âm (coordinates 18.09â²04.0 S and 178.27â²11.4 E). The 16S ribosomal RNA gene sequence (accession number Bankit 1235828) places this strain within the genus Streptomyces.
Fermentation and isolation of etamycin
Culture conditions
A 2âml frozen stock of actinomycete strain CNS-575 was used to inoculate 25âml A1 medium (10âg starch, 4âg yeast extract, 2âg peptone in 1âl seawater) in a 125âml Erlenmeyer flask and shaken at 27â°C. The 5-day-old seed culture was then used to inoculate a 1âl culture in A1bfe+C (A1 media described above, with the addition of 1âg CaCO3, 5âml of 2% (w/v) KBr stock solution and 5âml of 0.8% (w/v) Fe2(SO4)·4H2O stock solution). Lastly, 25âml aliquots of the 3-day-old 1âl culture were used to inoculate each of 18 1âl cultures in 2.8âl Fernbach flasks containing medium A1bfe+C. Strain CNS-575 was allowed to grow with shaking for 7 days at 27â°C before extraction.
Isolation
Column chromatography was carried out on silica gel (Selecto Scientific, Suwanee, GA, USA; particle size 63â200âÎŒm). HPLC was carried out on a Beckman System Gold liquid chromatograph (126) (Beckman-Coulter, Brea, CA, USA) equipped with a Shimadzu diode array detector (SPD-M10AVP) (Shimadzu Corporation, Kyoto, Japan) and using an Ultracarb ODS (30) column (Phenomenex, Torrance, CA, USA; 250 à 10.0âmm, 5âÎŒm particle size). A 40âl culture of strain CNS-575 was extracted using XAD-7 resin and eluted with acetone (2 à ). The acetone solvent was removed by evaporation, and the remaining water was extracted three times with ethyl acetate. The obtained extract (1.83âg) was fractionated on silica gel (step gradient from 100:1 to 5:1 dichloromethane: methanol eluent). Fractions with antibacterial activity were eluted with 50:1 and 20:1 dichloromethane: methanol. The active fractions (487âmg) were pooled and subjected to HPLC separation (isocratic, 60% aqueous acetonitrile eluent, 3âmlâminâ1 flow rate) to give etamycin (108.9âmg) after 31âmin.
Spectroscopic analysis of etamycin
All NMR spectra were measured using a Varian Oxford AS500 spectrometer (Varian Inc., Palo Alto, CA, USA) (5âmm double-resonance inverse broadband probe) at 500 and 125âMHz for 1H and 13C NMR, respectively. Offline processing was conducted using Mestre-C NMR Software (Mestrelab Research, La Coruña, Spain; http://www.mestrec.com). 1H and 13C chemical shifts were referenced with the CDCl3 solvent peaks at ÎŽ 7.26 and ÎŽ 77.0, respectively (Figure 1 and Table 1). The IR spectrum was recorded in KBr on a Nicolet IR100 FTIR spectrometer (Thermo, Waltham, MA, USA). The UV spectrum was recorded in methanol on a Beckman Coulter DU800 spectrophotometer (Beckman-Coulter). The optical rotation was measured in methanol on a Jasco P-2000 polarimeter (Jasco, Inc., Easton, MD, USA). HR-MS were acquired at the UCSD Molecular Mass Spectrometry Facility.
Susceptibility testing
Minimum inhibitory concentration (MIC) assays were performed by microbroth dilution. Briefly, an overnight culture of the test strain was diluted to 1:100 in broth and grown to mid-logarithmic phase at 37â°C in a shaking incubator. Todd-Hewitt broth (obtained from Hardy Diagnostics, Santa Maria, CA, USA) was used for all strains except H. influenzae, for which the susceptibility testing was carried out in brainâheart infusion media supplemented with hemin (10âÎŒgâmlâ1) and NAD (10âÎŒgâmlâ1). The culture was pelleted and resuspended to OD600=0.4 in phosphate-buffered saline. The bacteria were then added to a 96-well test plate containing etamycin dilutions in broth; the final bacterial concentration was 5 à 105âCFUâmlâ1 in a final volume of 100âÎŒl. Vancomycin (Hospira, Lake Forest, IL, USA) was used as a control for all MRSA strains. Other control antibiotics included: ampicillin (Sandoz, Princeton, NJ, USA) for E. faecalis and Streptococcus spp, cloramphenicol (Sigma Chemical, St Louis, MO, USA) for M. catarrhalis and H. influenzae, and ciprofloxacin (Bedford Laboratories, Bedford, OH, USA) for P. aeruginosa and E. coli. The test plate containing the etamycin dilutions and target bacteria was incubated at 37â°C in a shaking incubator for 18â20âh. MIC was determined to be the lowest concentration of etamycin that inhibited bacterial growth as determined by turbidometric assessment at OD600.
Cytotoxicity assay
Analysis of mammalian cell cytotoxicity was carried out essentially as described.15 Briefly, etamycin dilutions were added to HeLa cells (2 Ã 104âcells per well) in a sterile 96-well plate. Cytotoxicity was assayed by MTS at 24âh using the CellTiter 96 AQueous non-radioactive cell proliferation assay according to the manufacturer's instructions (Promega, Madison, WI, USA). Plates were read at A490 in a Versamax microplate reader (Molecular Devices, Mountain View, CA, USA).
In vitro time-kill assays
Time-kill assays were carried out as previously described16, 17, 18 with modifications. Briefly, MRSA strain USA300 (UAMS-1182) was cultured overnight at 37â°C in Todd-Hewitt broth. The bacteria were then grown to mid-logarithmic phase and prepared in phosphate-buffered saline as for the MIC assays. Test tubes were prepared in duplicate containing vehicle control, etamycin, or vancomycin at concentrations of 1, 4 or 10 times the MIC of 4âÎŒgâmlâ1 and 1.5âÎŒgâmlâ1, respectively. Bacteria were added to each tube at a starting inoculum of âŒ5 à 105âCFUâmlâ1 in a final volume of 5âml. The cultures were incubated in a 37â°C shaking incubator, and aliquots were removed from each test tube at 0, 3, 6 and 24âh. These aliquots were serially diluted in phosphate-buffered saline and plated on Todd-Hewitt agar plates in the absence of antibiotics. Colonies were enumerated after 24âh at 37â°C. The limit of detection of this assay equated to 40âCFUâmlâ1.
In vivo studies
Female CD1 mice (8 weeks old) (Charles River, Wilmington, MA, USA) were injected i.p. with 2 Ã 109âCFU of HA-MRSA strain Sanger 252 in 4% mucin. At 1 and 8âh after infection, the mice were injected i.p. with etamycin (20âmgâkgâ1) or an equivalent volume of vehicle control. The mice were observed for signs of sepsis, including lethargy, piloerection and dehydration. Survival was monitored for a total of 72âh, and mice that became moribund during the study were humanely euthanized, as were any surviving mice at the end of the study. These studies were reviewed and approved by the University of California San Diego Animal Subjects Committee.
Results
The actinomycete strain designated CNS-575 was cultured from a marine sediment collected along the coast of Fiji. The 16S rRNA gene sequence analysis places this strain within the genus Streptomyces; however, it shares <98% sequence identity with any formally described species, suggesting that it may represent a new species. Solid-phase extraction and chromatographic purification yielded an 879 molecular weight peptide, which appeared as three distinct rotamers in the NMR spectrum. The presence of rotamers was not affected by changes of NMR solvent or acquisition temperature, and we ultimately performed the NMR-based structure elucidation of all three rotamers simultaneously (Table 1). The resulting planar structure was consistent with that of the previously reported terrestrially derived streptogramin, etamycin19, 20 (Figure 1), although a complete chemical shift assignment of etamycin has not previously been reported. Acid hydrolysis followed by analysis using advanced Marfey's method21 revealed the stereochemical configuration to be consistent with etamycin (see Supplementary Information for a detailed discussion of the Marfey's analysis).
Etamycin was originally isolated from terrestrial actinomycete S. griseus alongside the streptogramin-A antibiotic griseoviridin, and the two molecules together displayed synergism common for other streptogramins, including bactericidal activity primarily against some Gram-positive organisms.22 We investigated in more detail the antibiotic properties of etamycin as a single agent against a panel of clinically relevant MRSA strains and discovered it to possess very good activity. MIC ranged from 1â2âmgâlâ1 for CA-MRSA strains and certain HA-MRSA strains, to 8-16âmgâlâ1 for the HA-MRSA strains ATCC 33591 and Sanger 252 (Table 2). Etamycin also showed a low level of activity against strains of the Gram-positive bacteria S. pyogenes and S. agalactiae (MIC=8âmgâlâ1) and minimal activity against vancomycin-resistant E. faecalis (MIC=16âmgâlâ1). The spectrum of etamycin activity extended to strains of the Gram-negative coccobacilli and respiratory tract pathogens M. catarrhalis (MIC=1âmgâlâ1) and to a lesser extent H. influenzae (MIC=16âmgâlâ1), but not to other Gram-negative pathogens tested including E. coli, S. typhimurium and P. aeruginosa. To assess a potential therapeutic index for etamycin against MRSA, we analyzed its effects in a mammalian HeLa cell cytotoxicity assay. We found that etamycin exhibited no cytoxicity at concentrations up to 128âmgâlâ1 (data not shown), suggesting the potential for a favorable therapeutic index against MRSA.
We studied the time-kill kinetics of etamycin at different concentrations for comparison against vancomycin, the most common agent used in current MRSA therapy. Evaluation was conducted at 1 Ã , 4 Ã and 10 Ã the respective MIC of each drug against the CA-MRSA (USA300) strain UAMS-1182 (Figure 2a). As shown in Figure 2b, etamycin killed UAMS-1182 somewhat more rapidly than vancomycin at 10 Ã MIC during the first 6âh; however, vancomycin was able to reduce the number of viable bacteria to below the threshold of detection of this assay by 24âh, whereas killing by etamycin, although substantial, remained incomplete. Collectively, these data suggest that etamycin acts in both time- and concentration-dependent mechanisms and is faster-acting but significantly less bactericidal than vancomycin.
As a first assessment of the in vivo efficacy of etamycin, we used a murine systemic infection model, conservatively choosing as the challenge strain MRSA Sanger 252, against which etamycin exhibited a higher MIC of 8â16âmgâlâ1. Female CD1 mice (8 weeks old) were treated with etamycin (20âmgâkgâ1) or vehicle control at 1 and 8âh after i.p. MRSA infection, and general health and survival were followed up for up to 72âh. Within 6â12âh of MRSA infection, most mice in the control group exhibited signs of sepsis including lethargy, dehydration, and piloerection, whereas the severity of these symptoms was greatly reduced or absent in most of the etamycin-treated animals. As shown in Figure 3, the two doses of etamycin offered significant protection to mice infected with MRSA during the 72âh monitoring period (20% mortality vs 75% morality in controls).
Discussion
For the first time from a marine-derived actinomycete we isolated the streptogramin, etamycin, and found that it had marked antibacterial activity against clinically important strains of MRSA. Although etamycin was first identified more than five decades ago, its antibiotic properties had not been characterized in detail. Those early studies also describe etamycin as a bacteriostatic compound against the S. aureus strain Duncan.22 We found that etamycin alone at high concentration may exhibit limited bactericidal activity against contemporary CA-MRSA strains, as after 24âh of exposure to etamycin at 4 Ã MIC, < 1% of the starting inoculum of CA-MRSA strain UAMS1182 remained viable (Figure 2), representing an approximate three-log kill by 24âh. Furthermore, the progressive decrease in the number of surviving bacteria over time even at 1 Ã MIC also supports a conclusion that etamycin exhibits some, although certainly incomplete, bactericidal activity over 24âh. However, despite this result, etamycin clearly does not kill CA-MRSA as effectively as vancomycin in the 24-h assay.
The streptogramin antibiotic, quinupristin-dalfopristin (Synercid) is currently approved for use in complicated cutaneous infections caused by multiple drug-resistant Gram-positive cocci, including MRSA and vancomycin-resistant Enterococcus faecium.23 In this combination, streptogramins A and B together act to inhibit protein synthesis, with quinupristin (A) preventing polypeptide elongation by binding to the 50S ribosomal subunit and dalfopristin (B) binding to another site on the 50S ribosomal subunit and inducing a conformational change enhancing quinupristin activity.24 In our assays we found that etamycin, presumably through protein synthesis inhibition, exhibits significant activity against MRSA alone without the benefit of its streptogramin A partner. Etamycin also demonstrated a very low level of activity against E. faecalis (MIC=16âmgâlâ1), a frequently drug-resistant nosocomial pathogen that is associated with urinary tract and surgical wound infections,25 and this activity seemed comparable to the very low level of activity of Synercid against this pathogen in our assay. Clearly, if etamycin were ever considered for introduction into clinical use, defined breakpoints would need to be determined.
Our isolation of etamycin from a novel marine-derived actinomycete species emphasizes the utility of the marine environment as a source of antibacterials,7 in particular marine-derived actinomycetes that have been shown to naturally produce a wide variety of active parent molecules and analogs.6, 11, 26 Etamycin was previously isolated from the terrestrial microbe, S. griseoviridus, naturally occurring as multiple congeners in which a hydroxyproline residue was replaced by a proline.27 In addition, limited structureâactivity relationship data point to the importance of the picolinyl moiety of etamycin for its activity.19 Future studies will further investigate the activity of etamycin analogs from marine-derived actinomycetes for characterization and optimization of activity toward further preclinical development.
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Acknowledgements
This work was supported by the National Institutes of Health Training Program in Marine Biotechnology (T32 GM067550 to NH), by the National Institutes of Health (RO1GM084350-01 to WF and VN), by the National Institutes of Health International Cooperative Biodiversity Groups program (U01-TW007401-01 to WF and PRJ), and by the National Institutes of Health Cancer Therapeutics Training Program (T32 CA121938 to KNM). We gratefully acknowledge R Raju and B Aalbersberg for providing the actinomycete strain CNS-575. We also acknowledge George Sakoulas, MD, for many helpful discussions.
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Haste, N., Perera, V., Maloney, K. et al. Activity of the streptogramin antibiotic etamycin against methicillin-resistant Staphylococcus aureus. J Antibiot 63, 219â224 (2010). https://doi.org/10.1038/ja.2010.22
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DOI: https://doi.org/10.1038/ja.2010.22
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