Featured
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Detecting organelle-specific activity of potassium channels with a DNA nanodevice
A potassium activity reporter detects organelle-specific channel function.
- Palapuravan Anees
- , Anand Saminathan
- & Yamuna Krishnan
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Research Briefing |
Electroosmotic flow across nanopores for single-molecule protein sequencing
By using fixed charges to engineer a strong electroosmotic flow, we achieve the unidirectional transport of natural polypeptides across nanopores. Our approach enables native proteins to be transported enzymatically and non-enzymatically in the absence of denaturant and electrophoretic tags, with potential applications for protein sequencing.
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Article |
Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force
An engineered nanopore translocates untagged full-length proteins by electroosmotic force.
- Adina Sauciuc
- , Blasco Morozzo della Rocca
- & Giovanni Maglia
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Article |
A microneedle vaccine printer for thermostable COVID-19 mRNA vaccines
Automated fabrication of microneedle patch mRNA vaccines for COVID-19 may improve vaccine access.
- Aurélien vander Straeten
- , Morteza Sarmadi
- & Ana Jaklenec
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Research Briefing |
Augmenting electron microscopy with barcoded gene reporters
Genetically encoded concentric barcodes function as multiplexed electron microscopy (EM) gene reporters (EMcapsulins) for cell culture and in vivo models. These barcodes can be used to visualize gene expression information as automatically labeled overlays on volume EM data.
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Article
| Open Access
Genetically encoded barcodes for correlative volume electron microscopy
Multiplexable barcodes for electron microscopy are applied to brain imaging.
- Felix Sigmund
- , Oleksandr Berezin
- & Gil Gregor Westmeyer
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Article |
Unidirectional single-file transport of full-length proteins through a nanopore
Full-length, unfolded proteins are slowly translocated through nanopores without enzymes and fingerprinted.
- Luning Yu
- , Xinqi Kang
- & Meni Wanunu
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Research Briefing |
Real-time monitoring of tumor-homing bacteria and tumor cells in vivo using ultrasound
By screening natural prokaryotic gas vesicle gene clusters, we found and engineered new acoustic reporter genes (ARGs) that give bacteria and mammalian cells brighter ultrasound contrast for real-time noninvasive imaging. Expressing these ARGs in engineered cells enabled us to image tumor-homing bacteria and perform genetically guided tumor biopsies in vivo.
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Article |
RNA-responsive elements for eukaryotic translational control
The translation of transgenes is regulated using RNA sensors.
- Evan M. Zhao
- , Angelo S. Mao
- & James J. Collins
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Article |
Purification of multiplex oligonucleotide libraries by synthesis and selection
Accurate oligonucleotide libraries are produced by synthesis and selection.
- Hansol Choi
- , Yeongjae Choi
- & Sunghoon Kwon
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Article |
Monitoring deep-tissue oxygenation with a millimeter-scale ultrasonic implant
The oxygenation of deep tissues is continuously measured using an ultrasound-powered wireless implant.
- Soner Sonmezoglu
- , Jeffrey R. Fineman
- & Michel M. Maharbiz
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Correspondence |
Research priorities for COVID-19 sensor technology
- Allison Tong
- , Tania C. Sorrell
- & Hans Zoellner
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News & Views |
Reading amino acids in a nanopore
In a step toward nanopore sequencing of proteins, an aerolysin pore discriminates many of the proteinogenic amino acids.
- Stefan Howorka
- & Zuzanna S. Siwy
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Letter |
Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency
Precise genome editing is made more efficient by stabilizing Cas9 and enhancing shuttling to the nucleus.
- David N. Nguyen
- , Theodore L. Roth
- & Alexander Marson
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Article |
Increasing the accuracy of nanopore DNA sequencing using a time-varying cross membrane voltage
The accuracy of nanopore DNA sequencing is substantially improved by application of a variable-voltage sequencing mode.
- Matthew T. Noakes
- , Henry Brinkerhoff
- & Jens H. Gundlach
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Article |
Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts
A ribozyme-based circularization strategy improves the stability of exogenously expressed RNA aptamers.
- Jacob L. Litke
- & Samie R. Jaffrey
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Letter |
Real-time measurement of protein–protein interactions at single-molecule resolution using a biological nanopore
Single-molecule analyses of transient protein–protein interactions are enabled using an engineered biological nanopore.
- Avinash Kumar Thakur
- & Liviu Movileanu
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News & Views |
Smart cancer therapy with DNA origami
Tumor infarction is achieved through targeted delivery of thrombin by DNA nanorobots.
- Ennio Tasciotti
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Article |
Virus stamping for targeted single-cell infection in vitro and in vivo
Viruses are transduced to single cells in tissues, organoids and in the mouse brain using mechanical carriers.
- Rajib Schubert
- , Stuart Trenholm
- & Botond Roska
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Article |
Molecular afterglow imaging with bright, biodegradable polymer nanoparticles
Ultra-high signal-to-background in vivo imaging is enabled by biocompatible semiconducting polymer nanoparticles.
- Qingqing Miao
- , Chen Xie
- & Kanyi Pu
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Article |
Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo
Protein origami using coiled-coil building blocks produces self-assembling polyhedral cages for diverse applications.
- Ajasja Ljubetič
- , Fabio Lapenta
- & Roman Jerala
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News & Views |
Manufacture of CAR-T cells in the body
Engineered T cells for cancer therapy are produced in the mouse bloodstream, avoiding the need to harvest T cells and manipulate them ex vivo.
- Johanna Olweus
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News & Views |
Automated design of DNA origami
A top-down computational method enables fabrication of DNA objects in any shape.
- Veikko Linko
- & Mauri A Kostiainen
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Letter |
An injectable nanoparticle generator enhances delivery of cancer therapeutics
A silicon-based microparticle that delivers doxorubicin nanoparticles improves the efficacy of breast cancer chemotherapy.
- Rong Xu
- , Guodong Zhang
- & Haifa Shen
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News & Views |
Big thinking for adjuvants
Particles formed by polymeric adjuvants preferentially localize to the lymph node and elicit robust immunity.
- Eric L Dane
- & Darrell J Irvine
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Article |
In vivo characterization of the physicochemical properties of polymer-linked TLR agonists that enhance vaccine immunogenicity
Vaccine efficacy is enhanced by optimizing the design of polymeric particles for adjuvant and antigen delivery.
- Geoffrey M Lynn
- , Richard Laga
- & Robert A Seder
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Article |
Single-cell ChIP-seq reveals cell subpopulations defined by chromatin state
Chromatin state is analyzed for the first time in single cells, revealing new cell subpopulations.
- Assaf Rotem
- , Oren Ram
- & Bradley E Bernstein
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Brief Communication |
Subangstrom single-molecule measurements of motor proteins using a nanopore
The movement of single motor proteins on DNA is measured at subangstrom resolution using a nanopore.
- Ian M Derrington
- , Jonathan M Craig
- & Jens H Gundlach
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Perspective |
Microfluidic organs-on-chips
Organ-level physiology is recapitulated in vitro by culturing cells in perfused, microfluidic devices.
- Sangeeta N Bhatia
- & Donald E Ingber
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News & Views |
Next-generation wearable electronics
New fabric-like sensors measure and transmit mechanical strain on the skin with unprecedented sensitivity.
- Michael J Cima
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Letter |
Decoding long nanopore sequencing reads of natural DNA
Laszlo et al. demonstrate sequence alignment, and proof-of-concept organism identification, genome assembly and polymorphism detection from nanopore analysis of natural DNA.
- Andrew H Laszlo
- , Ian M Derrington
- & Jens H Gundlach
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Article |
Real-time imaging of oxidative and nitrosative stress in the liver of live animals for drug-toxicity testing
Semiconducting polymer nanoparticles are used to image toxic reactive oxygen and reactive nitrogen species in live mice.
- Adam J Shuhendler
- , Kanyi Pu
- & Jianghong Rao
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Brief Communication |
Single-molecule site-specific detection of protein phosphorylation with a nanopore
A protein nanopore can distinguish model proteins phosphorylated at adjacent sites.
- Christian B Rosen
- , David Rodriguez-Larrea
- & Hagan Bayley
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News & Views |
Cages from coils
The use of coiled coils could facilitate the modular, predictable design of protein nanocages.
- Bryan S Der
- & Brian Kuhlman
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News & Views |
A window onto siRNA delivery
Two studies trace the intracellular trafficking of siRNA delivered by lipid nanoparticles.
- Yuhua Wang
- & Leaf Huang
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Letter |
Unfoldase-mediated protein translocation through an α-hemolysin nanopore
Proteins are denatured and threaded through a nanopore by a bacterial unfoldase, allowing certain features of the protein sequence to be detected.
- Jeff Nivala
- , Douglas B Marks
- & Mark Akeson
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Patents |
Recent patent applications in biosensors
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News & Views |
Channeling DNA for optical mapping
Stretching DNA molecules in nanochannels allows structural and copy-number variations to be visualized like beads on a string.
- Yael Michaeli
- & Yuval Ebenstein
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Article |
Genome mapping on nanochannel arrays for structural variation analysis and sequence assembly
Optical maps of a genome, which are generated by imaging labeled single molecules of DNA, facilitate structural variation analysis and sequence assembly. Lam et al. immobilize DNA molecules in nanoscale channels, increasing the accuracy and throughput of the mapping process.
- Ernest T Lam
- , Alex Hastie
- & Pui-Yan Kwok
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Article |
Tandem fluorescent protein timers for in vivo analysis of protein dynamics
Khmelinskii et al. describe tandem fluorescent protein timers for measuring protein turnover and trafficking in living cells. Data from a single time point are used to determine protein stability, allowing the authors to screen for components of protein degradation pathways.
- Anton Khmelinskii
- , Philipp J Keller
- & Michael Knop
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News & Views |
Nanopores as protein sensors
Two studies describe progress in designing nanopores capable of detecting single protein molecules.
- Stefan Howorka
- & Zuzanna S Siwy
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A DNA nanodevice for mapping sodium at single-organelle resolution
Ablynx's nanobody fragments go places antibodies cannot
Turning silver dust into electronics
Stretching sensors by confinement
Membrane channels built from DNA
Proof of concept for next-generation nanoparticle drugs in humans