Membrane trafficking articles within Nature Communications

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  • Article
    | Open Access

    BMP ligands act as retrograde signalling molecules to regulate presynaptic development, and regulation of BMP receptors by endocytosis may be an important component of this signalling pathway. Here, the authors show that Abi-mediated macropinocytosis of BMP receptors in Drosophila larva and contributes to neuromuscular development.

    • Najin Kim
    • , Sungdae Kim
    •  & Seungbok Lee

  • Article
    | Open Access

    The architecture of functional TNTs is still under debate. Here, the authors combine correlative FIB-SEM, light- and cryo-electron microscopy approaches to elucidate the structure of TNTs in neuronal cells, showing that they form structures that are distinct form other membrane protrusions.

    • Anna Sartori-Rupp
    • , Diégo Cordero Cervantes
    •  & Chiara Zurzolo

  • Article
    | Open Access

    Drp1 and Dnm2 have been implicated in mitochondrial fission events, although their specific activities in constriction and scission have been unclear. Here, the authors demonstrate that Drp1 is sufficient to constrict and sever mitochondrial and peroxisomal membranes in the absence of Dnm proteins.

    • Sukrut C. Kamerkar
    • , Felix Kraus
    •  & Michael T. Ryan

  • Article
    | Open Access

    The GTPase dynamin catalyzes membrane fission but activity of the dynamin-related ATPase EHD1 is unknown. Here, using in vitro reconstitution assays and molecular dynamics simulations, the authors report that EHD1 hydrolyzes ATP to remodel, causing fission of membrane tubes and that this is necessary for endocytic recycling.

    • Raunaq Deo
    • , Manish S. Kushwah
    •  & Thomas J. Pucadyil

  • Article
    | Open Access

    Trastuzumab binding to tumor cells depends on the availability of HER2 at the cell membrane. Here the authors show that caveolin-1 (CAV1) regulates HER2 density at the cell membranes and that CAV1 gene knockdown or protein depletion via the cholesterol modulator lovastatin, increases trastuzumab binding and anti-tumor activity.

    • Patrícia M. R. Pereira
    • , Sai Kiran Sharma
    •  & Jason S. Lewis

  • Article
    | Open Access

    Exocyst complex tethers vesicles to plasma membranes, but assembly mechanisms remain unclear. Here, the authors use Cas9 gene editing to tag exocyst components in epithelial cells, and find that exocyst subcomplexes are recruited to membranes independently, but are both needed for vesicle fusion.

    • Syed Mukhtar Ahmed
    • , Hisayo Nishida-Fukuda
    •  & Ian G. Macara

  • Article
    | Open Access

    Some p53 mutants promote invasive migration of cancer cells and metastasis of tumours in vivo. However the key mechanistic details behind these phenomena remain unclear. Here the authors propose a non-cell autonomous mechanism involving fibroblasts, whereby mutant p53-expressing cancer cells activate an exosome-mediated mechanism that influences integrin recycling in fibroblasts, thus influencing extracellular matrix remodelling to favour cancer cell invasion and migration.

    • David Novo
    • , Nikki Heath
    •  & Jim C. Norman

  • Article
    | Open Access

    During de novo establishment of apical-basal polarity, a basolateral membrane must be converted into an apical delivery zone. Here, the authors use MDCK 3D cysts to uncover that the phospholipid PI(3,4)P2 is an apical membrane determinant.

    • Álvaro Román-Fernández
    • , Julie Roignot
    •  & David M. Bryant

  • Article
    | Open Access

    The COPII coat assembles in two concentric layers and mediates protein export from the endoplasmic reticulum. Here the authors present the 4.9 Å resolution cryo-tomography and subtomogram averaging structure of the membrane bound COPII inner coat that was obtained by in vitro reconstitution and discuss mechanistic implications.

    • Joshua Hutchings
    • , Viktoriya Stancheva
    •  & Giulia Zanetti

  • Article
    | Open Access

    The nature of the linker is known to affect the efficacy of antibody–drug conjugate (ADC). Here the authors show cetuximab-guided Avidin-Nucleic-Acid-Nanoassemblies to be superior to cetuximab-doxorubicin conjugate, and show its efficacy in KRAS mutant breast cancer, allowing for therapeutic expansion of anti-EGFR therapy.

    • Francesco Roncato
    • , Fatlum Rruga
    •  & Margherita Morpurgo

  • Article
    | Open Access

    Exosomes mediate cell-to-cell communication by transporting proteins, mRNAs, and miRNAs but the mechanisms of protein sorting to exosomes are poorly understood. Here, the authors uncover that ubiquitin-like 3 (UBL3) regulates protein sorting to exosomes by acting as a posttranslational modification.

    • Hiroshi Ageta
    • , Natsumi Ageta-Ishihara
    •  & Kunihiro Tsuchida

  • Article
    | Open Access

    Rab11 GTPases are involved in various cellular processes but their activation by guanine nucleotide exchange factors (GEFs) is not fully understood. Here, the authors present a structural and biochemical analysis of Rab11 bound to the GEF SH3BP5, providing insights how Rab-GEF specificity is achieved.

    • Meredith L. Jenkins
    • , Jean Piero Margaria
    •  & John E. Burke

  • Article
    | Open Access

    Sustained Wnt secretion requires the endosomal SNX3-retromer complex for endosome-to-trans-Golgi network transport of the internalised Wnt chaperone Wntless. Here the authors show that in both C. elegans and human cells, SNX3-retromer requires an evolutionary conserved membrane remodelling complex for Wntless sorting and Wnt secretion.

    • Ian J. McGough
    • , Reinoud E. A. de Groot
    •  & Peter J. Cullen

  • Article
    | Open Access

    The endosomal sorting complex required for transport-III (ESCRT-III) has been implicated in the packaging of HIV and HSV-1 viruses in the cytoplasm. Here the authors show that ESCRT-III proteins are required for the transport of HSV-1 nucleocapsids from nucleoplasm to cytosol through the nuclear envelope and confirm that the same mechanism is also used for the nucleocytoplasmic transport of RNP in Drosophila cells.

    • Jun Arii
    • , Mizuki Watanabe
    •  & Yasushi Kawaguchi

  • Article
    | Open Access

    Intraluminal vesicles are formed by the endosomal sorting complex required for transport (ESCRT) machinery. Here, the authors unravel the timing of vesicle budding, and that endosomal clathrin regulates concerted recruitment of ESCRT subcomplexes, required for efficient membrane remodeling.

    • Eva Maria Wenzel
    • , Sebastian Wolfgang Schultz
    •  & Camilla Raiborg

  • Article
    | Open Access

    During autophagy, phagophores elongate to form double-membrane vesicles but the mechanism behind their closure is unknown. Here, the authors develop an autophagy assay and find a role for the endosomal sorting complexes required for transport component CHMP2A as a phagophore closure regulator.

    • Yoshinori Takahashi
    • , Haiyan He
    •  & Hong-Gang Wang

  • Article
    | Open Access

    Several endocytic pathways operate simultaneously at the cell surface, including the clathrin and dynamin-independent CLIC/GEEC (CG) pathway. Here the authors show that small GTPases and BAR domain proteins regulate branched actin to make clathrin and dynamin-independent endocytic vesicles.

    • Mugdha Sathe
    • , Gayatri Muthukrishnan
    •  & Satyajit Mayor

  • Article
    | Open Access

    Membrane fusion and fission events at exoplasmic membrane surfaces are not well understood. Here the authors show that the C. elegans cell–cell fusogen AFF-1 is required for endocytic scission and apically-directed membrane trafficking during the development of a unicellular tube.

    • Fabien Soulavie
    • , David H. Hall
    •  & Meera V. Sundaram

  • Article
    | Open Access

    Endocytosis of T cell receptors (TCR) and their polarized recycling back to the plasma membrane is crucial for T cell activation; however, the underlying mechanisms remain unclear. Here the authors follow TCR and show that a mobile endocytic network connects clathrin-independent receptor endocytosis to recycling which is required for T cell activation.

    • Ewoud B. Compeer
    • , Felix Kraus
    •  & Jérémie Rossy

  • Article
    | Open Access

    Glucagon-like peptide-1 receptor (GLP-1R) promotes insulin secretion from pancreatic beta cells and undergoes agonist-mediated endocytosis. Here, authors study GLP-1R endocytosis caused by different agonists and show that a longer plasma membrane retention time of GLP-1R results in greater long-term insulin release.

    • Ben Jones
    • , Teresa Buenaventura
    •  & Stephen R. Bloom

  • Article
    | Open Access

    The CORVET complex is a multi-subunit complex that regulates fusion between early endosomes. Here the authors show that the CORVET subunits Vps3 and Vps8 also regulate vesicular transport from early to recycling endosomes and are required for recycling integrins to the plasma membrane.

    • Caspar T. H. Jonker
    • , Romain Galmes
    •  & Judith Klumperman

  • Article
    | Open Access

    Vacuolar sorting receptors (VSRs) are suggested to efficiently transport hydrolases by continuous cycling. Here, the authors use a nanobody-epitope interaction-based labeling approach to trace VSR recycling from the TGN/EE to the cis-Golgi and reveal ligand reloading of recycled VSRs.

    • Simone Früholz
    • , Florian Fäßler
    •  & Peter Pimpl

  • Article
    | Open Access

    Trafficking from endosomes to the trans-Golgi network requires recognition of vesicle tethers during membrane docking. Here, the authors identify a complex localised to AP-1 generated vesicles containing WDR11, C17orf75 and FAM91A, which together with TBC1D23 facilitates vesicle capture on Golgi membranes

    • Paloma Navarro Negredo
    • , James R. Edgar
    •  & Margaret S. Robinson

  • Article
    | Open Access

    The yeast plasma membrane consists of membrane microdomains with distinct protein composition. Here the authors use high-resolution single molecule imaging to observe diffusion of specific transmembrane proteins in and out of these microdomains, and propose features that dictate their inclusion/exclusion from these structures.

    • Frans Bianchi
    • , Łukasz Syga
    •  & Bert Poolman

  • Article
    | Open Access

    Two distinct and opposing models for clathrin-mediated endocytosis have been inferred from EM and structural biology data. Here the authors develop an optical method to directly visualize membrane-bending dynamics and show that coat assembly accommodates membrane bending during or after the assembly of the clathrin lattice, which is not predicted by either model.

    • Brandon L. Scott
    • , Kem A. Sochacki
    •  & Adam D. Hoppe

  • Article
    | Open Access

    Adapter proteins assist clathrin coated pit assembly. Here, the authors combine native mass spectrometry, crystallography and SAXS measurements and show that the membrane–proximal domains of the adaptor proteins epsin and Sla2 form complexes mediated through phosphatidylinositol 4,5-bisphosphate interfaces leading to assembly formation.

    • Maria M. Garcia-Alai
    • , Johannes Heidemann
    •  & Rob Meijers

  • Article
    | Open Access

    Neurodegeneration is increasingly associated with endolysosomal and autophagy dysfunction. Here, Miranda and colleagues show that disruption of neuronal PI3P/Vps34 signaling leads to endolysosomal membrane damage and aberrant release of undigested material in APP-CTF- and BMP-positive exosomes.

    • André M. Miranda
    • , Zofia M. Lasiecka
    •  & Gilbert Di Paolo

  • Article
    | Open Access

    Glutamine metabolism is well known to support tumour growth. Here the authors show that cancer cells also utilize glutamine to promote invasiveness by converting it to glutamate, which upon secretion activates metabotropic glutamate receptors to stimulate matrix metalloproteases recycling to the cell surface.

    • Emmanuel Dornier
    • , Nicolas Rabas
    •  & Jim C. Norman

  • Article
    | Open Access

    Impaired turnover of TDP-43 by impaired autophagy or proteasomal function have been suggested to be the cause of TDP-43 accumulation, a hallmark of ALS. Here the authors demonstrate that endocytosis is also important for regulating TDP-43 turnover and toxicity.

    • Guangbo Liu
    • , Alyssa N. Coyne
    •  & J. Ross Buchan

  • Article
    | Open Access

    Lysosomal integral membrane protein-2 (LIMP-2) is a glucocerebrosidase receptor, which is linked to kidney failure and other diseases. Here the authors show that LIMP-2 is also a phospholipid receptor and present the lipid-bound structure of the LIMP-2 luminal domain dimer and discuss its lipid trafficking mechanism.

    • Karen S. Conrad
    • , Ting-Wen Cheng
    •  & Michael D. Ehlers

  • Article
    | Open Access

    Sortilin is an endocytosis receptor with a luminal β-propeller domain. Here the authors present the structures of the β-propeller domain at neutral and acidic pH, which reveal that sortilin dimerises and undergoes conformational changes at low pH and further propose a model for low pH-induced ligand release by endocytosis receptors.

    • Nadia Leloup
    • , Philip Lössl
    •  & Bert J. C. Janssen

  • Article
    | Open Access

    The role of annexins in cell membrane repair is largely undefined. Here the authors use a model lipid bilayer to show that annexin A4 induces curvature at the membrane free edge and annexin A6 induces constriction force, and find that both annexins are recruited to wound edges in cells and are required for repair.

    • Theresa Louise Boye
    • , Kenji Maeda
    •  & Jesper Nylandsted

  • Article
    | Open Access

    Lysosomal distribution is linked to the role of lysosomes in many cellular functions. Here the authors show that the lysosomal protein TMEM55B is regulated by TFEB and recruits JIP4 to the lysosomal surface inducing dynein-dependent transport of lysosomes toward the cell center in response to stress conditions.

    • Rose Willett
    • , José A. Martina
    •  & Rosa Puertollano

  • Article
    | Open Access

    Existing pH-sensitive red fluorescent protein probes don’t perform well in monitoring exocytosis and endocytosis. Here, the authors combine organic dyes with self-labeling tags or antibodies to develop semisynthetic protein conjugates that can image synaptic vesicle fusion events in living cells.

    • Magalie Martineau
    • , Agila Somasundaram
    •  & David Perrais

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