Featured
-
-
Article
| Open Access
A multivesicular body-like organelle mediates stimulus-regulated trafficking of olfactory ciliary transduction proteins
Odor stimuli are transduced in cilia of olfactory sensory neurons. Here the authors identify an odor stimulus-regulated organelle that specifically stores and releases transduction proteins in the dendrite.
- Devendra Kumar Maurya
- , Anna Berghard
- & Staffan Bohm
-
Article
| Open Access
SNX19 restricts endolysosome motility through contacts with the endoplasmic reticulum
Endoplasmic reticulum (ER)-interorganelle membrane contact sites have emerged as key regulators of organelle dynamics. Here, the authors report that the ER-resident protein SNX19 mediates ER-endolysosome membrane contacts to maintain the perinuclear distribution of endolysosomes and restrict their motility.
- Amra Saric
- , Spencer A. Freeman
- & Juan S. Bonifacino
-
Article
| Open Access
KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a
Exosomes are intercellular signaling vesicles created by fusion of multivesicular bodies (MVBs) and the plasma membrane (PM), but secretory regulation is ill-defined. Song et al. show that KIBRA controls exosome secretion by protecting Rab27a from proteasomal degradation, promoting MVB-PM docking.
- Lin Song
- , Shi Tang
- & Yifeng Du
-
Article
| Open Access
The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation
Cell surface receptors are thought to be internalized via the multivesicular bodies (MVBs) in an ESCRT-dependent pathway. Here, the authors report that in yeast, a hexose transporter is internalized via an ESCRT-independent pathway into intralumenal fragments (ILF).
- Erin Kate McNally
- & Christopher Leonard Brett
-
Article
| Open Access
UBL3 modification influences protein sorting to small extracellular vesicles
Exosomes mediate cell-to-cell communication by transporting proteins, mRNAs, and miRNAs but the mechanisms of protein sorting to exosomes are poorly understood. Here, the authors uncover that ubiquitin-like 3 (UBL3) regulates protein sorting to exosomes by acting as a posttranslational modification.
- Hiroshi Ageta
- , Natsumi Ageta-Ishihara
- & Kunihiro Tsuchida
-
Article
| Open Access
A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis
The ESCRT complex contributes to selective vacuolar degradation of membrane proteins. Here, the authors show that the plant-specific BRAF protein negatively regulates intraluminal vesicle formation and membrane protein degradation by preventing FREE1 binding to the ESCRT-I complex protein Vps23.
- Jinbo Shen
- , Qiong Zhao
- & Liwen Jiang
-
Article
| Open Access
Ist1 regulates ESCRT-III assembly and function during multivesicular endosome biogenesis in Caenorhabditis elegans embryos
During the oocyte-to-embryo transition in C. elegans, multivesicular endosomes (MVEs) are formed rapidly. Using live imaging and electron microscopy, the authors here show that the ESCRT-III subunit Ist1 is required for normal MVE biogenesis in the early C. elegans embryo.
- E. B. Frankel
- , Raakhee Shankar
- & Anjon Audhya
-
Article
| Open Access
Exosome-delivered microRNAs modulate the inflammatory response to endotoxin
Response to inflammatory stimuli such as endotoxin is coordinated at many levels. Here, the authors show that two microRNAs known to regulate inflammatory response inside the cell are secreted by dendritic cells and modulate inflammatory signalling in the neighbouring cells.
- Margaret Alexander
- , Ruozhen Hu
- & Ryan M. O’Connell
-
Article
| Open Access
A balance between membrane elasticity and polymerization energy sets the shape of spherical clathrin coats
A relationship between membrane tension and clathrin polymerization during endocytosis has not been experimentally established. Here, the authors show using an in vitroreconstituted system and theoretical modelling that membrane tension regulates clathrin polymerization into spherical cages by varying the membrane budding energy.
- Mohammed Saleem
- , Sandrine Morlot
- & Aurélien Roux
A phosphoinositide switch mediates exocyst recruitment to multivesicular endosomes for exosome secretion