Article
|
Open Access
Featured
-
-
Article
| Open AccessESCRT-dependent STING degradation inhibits steady-state and cGAMP-induced signalling
STING is an intracellular sensor of pathogen- or host-derived DNA. In this study, the authors identify an ESCRT complex that regulates STING degradation, thus acting as a homeostatic regulator of STING signalling and type-I interferon responses.
- Matteo Gentili
- , Bingxu Liu
- & Nir Hacohen
-
Article
| Open AccessHRS phosphorylation drives immunosuppressive exosome secretion and restricts CD8+ T-cell infiltration into tumors
Lack of CD8+ T-cell infiltration into solid tumors is associated with poor responsiveness to immune checkpoint therapy (ICT). Here, the authors show that blocking the phosphorylation of HRS to reduce the induction of immunosuppressive exosomes promotes CD8+ T-cell infiltration into tumors and enhances the efficacy of ICT in mouse melanoma models.
- Lei Guan
- , Bin Wu
- & Wei Guo
-
Article
| Open AccessT-cell trans-synaptic vesicles are distinct and carry greater effector content than constitutive extracellular vesicles
T cells communicate with antigen-presenting cells (APC) via the signaling crosstalk at the immunological synapse (IS). Here the authors use bead-supported lipid bilayers as synthetic APCs to find that trans-synaptic vesicles produced by T cells in the IS carry specialized cargos distinct from constitutive extracellular vesicles to serve as intercellular messengers.
- Pablo F. Céspedes
- , Ashwin Jainarayanan
- & Michael L. Dustin
-
Article
| Open AccessAsgard archaea shed light on the evolutionary origins of the eukaryotic ubiquitin-ESCRT machinery
The ESCRT pathway is crucial for membrane remodelling in eukaryotes. Here, Hatano et al. explore the phylogeny, structure, and biochemistry of homologues of the ESCRT machinery and the associated ubiquitylation system in Asgard archaea, the closest living relatives of eukaryotes.
- Tomoyuki Hatano
- , Saravanan Palani
- & Mohan Balasubramanian
-
Article
| Open AccessSnf7 spirals sense and alter membrane curvature
Snf7 is the major component of the ESCRTIII membrane deformation system. Here, the authors used high-speed AFM to study Snf7 on nano-patterned and soft supports and show that loaded Snf7 spiral springs are curvature sensitive and deform membranes.
- Nebojsa Jukic
- , Alma P. Perrino
- & Simon Scheuring
-
Article
| Open AccessCoordination of fungal biofilm development by extracellular vesicle cargo
The fungal pathogen Candida albicans can release extracellular vesicles that promote biofilm formation and antifungal resistance. Here, Zarnowski et al. define functions for numerous vesicle cargo proteins in biofilm matrix assembly and drug resistance, as well as in fungal cell adhesion and dissemination.
- Robert Zarnowski
- , Andrea Noll
- & David R. Andes
-
Article
| Open AccessAn ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D−TSG101 interactions
Metabolic rewiring requires the mobilization of fatty acids (FA) from lipid droplets (LDs) at membrane contact sites (MCSs), although the details of FA transfer remain unclear. Here, the authors show that VPS13D and the ESCRT complex remodel LD membranes to promote FA trafficking to mitochondria.
- Jingru Wang
- , Na Fang
- & Wei-Ke Ji
-
Article
| Open AccessHuman ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation
ESCRT-III complexes assemble in vivo inside membrane structures with a negative Gaussian curvature, but how membrane shape influences ESCRT-III polymerization remains unclear. Here authors use structural and biophysical methods to show how human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation.
- Aurélie Bertin
- , Nicola de Franceschi
- & Patricia Bassereau
-
Article
| Open AccessThe intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation
Cell surface receptors are thought to be internalized via the multivesicular bodies (MVBs) in an ESCRT-dependent pathway. Here, the authors report that in yeast, a hexose transporter is internalized via an ESCRT-independent pathway into intralumenal fragments (ILF).
- Erin Kate McNally
- & Christopher Leonard Brett
-
Article
| Open AccessESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity
The endosomal sorting complex required for transport-III (ESCRT-III) has been implicated in the packaging of HIV and HSV-1 viruses in the cytoplasm. Here the authors show that ESCRT-III proteins are required for the transport of HSV-1 nucleocapsids from nucleoplasm to cytosol through the nuclear envelope and confirm that the same mechanism is also used for the nucleocytoplasmic transport of RNP in Drosophila cells.
- Jun Arii
- , Mizuki Watanabe
- & Yasushi Kawaguchi
-
Article
| Open AccessConcerted ESCRT and clathrin recruitment waves define the timing and morphology of intraluminal vesicle formation
Intraluminal vesicles are formed by the endosomal sorting complex required for transport (ESCRT) machinery. Here, the authors unravel the timing of vesicle budding, and that endosomal clathrin regulates concerted recruitment of ESCRT subcomplexes, required for efficient membrane remodeling.
- Eva Maria Wenzel
- , Sebastian Wolfgang Schultz
- & Camilla Raiborg
-
Article
| Open AccessAn autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure
During autophagy, phagophores elongate to form double-membrane vesicles but the mechanism behind their closure is unknown. Here, the authors develop an autophagy assay and find a role for the endosomal sorting complexes required for transport component CHMP2A as a phagophore closure regulator.
- Yoshinori Takahashi
- , Haiyan He
- & Hong-Gang Wang
-
Article
| Open AccessIst1 regulates ESCRT-III assembly and function during multivesicular endosome biogenesis in Caenorhabditis elegans embryos
During the oocyte-to-embryo transition in C. elegans, multivesicular endosomes (MVEs) are formed rapidly. Using live imaging and electron microscopy, the authors here show that the ESCRT-III subunit Ist1 is required for normal MVE biogenesis in the early C. elegans embryo.
- E. B. Frankel
- , Raakhee Shankar
- & Anjon Audhya
-
Article
| Open AccessCryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution
The ESCRT-III and Vps4 complexes mediate a variety of membrane remodelling events. Here the authors describe the structure of the Vps4 hexamer complexed to its cofactor Vta1, and show that Vta1 bridges adjacent Vps4 subunits to promote formation of the active hexamer during ESCRT-III filament disassembly.
- Shan Sun
- , Lin Li
- & Sen-Fang Sui
-
Article |
Mechanism of Ca2+-triggered ESCRT assembly and regulation of cell membrane repair
Mechanical activity predisposes muscle cell membranes to damage, which is repaired by a poorly defined Ca2+-dependent mechanism. Scheffer et al. describe the machinery for Ca2+-induced assembly of ESCRT III membrane remodelling complex at damaged cell membranes, which facilitates repair.
- Luana L. Scheffer
- , Sen Chandra Sreetama
- & Jyoti K. Jaiswal