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| Open AccessOrphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish
- Nadja R. Brun
- , Matthew C. Salanga
- & John J. Stegeman
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| Open AccessBroad spectrum of CRISPR-induced edits in an embryonic lethal gene
- Kayla T. B. Fuselier
- , J. Michael Salbaum
- & Claudia Kappen
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| Open AccessTargeted insertion of large genetic payloads using cas directed LINE-1 reverse transcriptase
- Femila Manoj
- , Laura W. Tai
- & Thomas E. Kuhlman
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| Open AccessExperimental strategies to achieve efficient targeted knock-in via tandem paired nicking
- Md. Lutfur Rahman
- , Toshinori Hyodo
- & Hiroyuki Konishi
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| Open AccessA selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines
- Reto Eggenschwiler
- , Thomas Gschwendtberger
- & Tobias Cantz
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| Open AccessPRIMA: a rapid and cost-effective genotyping method to detect single-nucleotide differences using probe-induced heteroduplexes
- Hiroyuki Kakui
- , Misako Yamazaki
- & Kentaro K. Shimizu
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| Open AccessConstruction of stable mouse artificial chromosome from native mouse chromosome 10 for generation of transchromosomic mice
- Satoshi Abe
- , Kazuhisa Honma
- & Yasuhiro Kazuki
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| Open AccessGeneration of knock-in lampreys by CRISPR-Cas9-mediated genome engineering
- Daichi G. Suzuki
- , Hiroshi Wada
- & Shin-ichi Higashijima
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| Open AccessCas9-mediated genome editing reveals a significant contribution of calcium signaling pathways to anhydrobiosis in Pv11 cells
- Yugo Miyata
- , Hiroto Fuse
- & Takahiro Kikawada
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| Open AccessThe β2Tubulin, Rad50-ATPase and enolase cis-regulatory regions mediate male germline expression in Tribolium castaneum
- Sher Afzal Khan
- , Emma Jakes
- & Zach N. Adelman
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| Open AccessTargeting chromosome trisomy for chromosome editing
- Takuya Abe
- , Yuya Suzuki
- & Kouji Hirota
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| Open AccessTargeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants
- Martina Carlessi
- , Lorenzo Mariotti
- & Silvia Gonzali
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| Open AccessThe Penn State Protein Ladder system for inexpensive protein molecular weight markers
- Ryan T. Santilli
- , John E. Williamson III
- & Song Tan
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| Open AccessRapid target validation in a Cas9-inducible hiPSC derived kidney model
- Yasaman Shamshirgaran
- , Anna Jonebring
- & Ryan Hicks
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| Open AccessshRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus
- Wenping Hu
- , Haixue Zheng
- & Ning Li
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| Open AccessElectrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei
- Sami Havukainen
- , Jonai Pujol-Giménez
- & Christopher P. Landowski
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| Open AccessGenetic engineering of porcine endothelial cell lines for evaluation of human-to-pig xenoreactive immune responses
- Ping Li
- , Julia R. Walsh
- & Burcin Ekser
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| Open AccessA transgenic female killing system for the genetic control of Drosophila suzukii
- Marc F. Schetelig
- , Jonas Schwirz
- & Ying Yan
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| Open AccessA reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models
- Wen-Hsin Liu
- , Kerstin Völse
- & Irmela Jeremias
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| Open AccessCRISPR mediated targeting of DUX4 distal regulatory element represses DUX4 target genes dysregulated in Facioscapulohumeral muscular dystrophy
- Sunny Das
- & Brian P. Chadwick
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| Open AccessHGK promotes metastatic dissemination in prostate cancer
- Sara Garcia-Garcia
- , Maria Rodrigo-Faus
- & Alvaro Gutierrez-Uzquiza
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| Open AccessEvaluation method for asymmetric uncertainty of quantitative polymerase chain reaction measurements of deoxyribonucleic acids with low copy number
- Unoh Ki
- , Takeru Suzuki
- & Hirotaka Unno
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| Open AccessCRISPR-mediated mutations in the ABC transporter gene ABCA2 confer pink bollworm resistance to Bt toxin Cry2Ab
- Jeffrey A. Fabrick
- , Dannialle M. LeRoy
- & Bruce E. Tabashnik
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| Open AccessHemiacetal-less rapamycin derivatives designed and produced by genetic engineering of a type I polyketide synthase
- Kei Kudo
- , Takehiro Nishimura
- & Kazuo Shin-ya
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| Open AccessDirect conversion of porcine primary fibroblasts into hepatocyte-like cells
- Mariane Fráguas-Eggenschwiler
- , Reto Eggenschwiler
- & Heiner Niemann
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| Open AccessIn vivo enrichment of busulfan-resistant germ cells for efficient production of transgenic avian models
- Young Min Kim
- , Kyung Je Park
- & Jae Yong Han
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| Open AccessThe VH framework region 1 as a target of efficient mutagenesis for generating a variety of affinity-matured scFv mutants
- Yuki Kiguchi
- , Hiroyuki Oyama
- & Norihiro Kobayashi
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| Open AccessDevelopment of a novel heterologous gene expression system using earthworms
- Shin-ichi Akazawa
- , Yu Machida
- & Hayato Murayama
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| Open AccessThe efficacy of CRISPR-mediated cytosine base editing with the RPS5a promoter in Arabidopsis thaliana
- Minkyung Choi
- , Jae-Young Yun
- & Sang-Tae Kim
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| Open AccessAn efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
- Jens Hamar
- & Dietmar Kültz
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| Open AccessNovel combination of CRISPR-based gene drives eliminates resistance and localises spread
- Nicky R. Faber
- , Gus R. McFarlane
- & Gregor Gorjanc
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| Open AccessCRISPR/Cas9-based targeting of fluorescent reporters to human iPSCs to isolate atrial and ventricular-specific cardiomyocytes
- Orlando Chirikian
- , William R. Goodyer
- & Sean M. Wu
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| Open AccessEpigenetic silencing directs expression heterogeneity of stably integrated multi-transcript unit genetic circuits
- Jan Zimak
- , Zachary W. Wagoner
- & Weian Zhao
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| Open AccessThe plant AlcR-pAlcA ethanol-inducible system displays gross growth artefacts independently of downstream pAlcA-regulated inducible constructs
- Ricardo S. Randall
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| Open AccessDeficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
- Hieu Hoang Trung
- , Toru Yoshihara
- & Takashi Kuramoto
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| Open AccessGenetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37 °C
- L. G. V. Fernandes
- , R. L. Hornsby
- & J. E. Nally
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| Open AccessA selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines
- Kathryn Rozen-Gagnon
- , Soon Yi
- & Charles M. Rice
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| Open AccessCombined lentiviral- and RNA-mediated CRISPR/Cas9 delivery for efficient and traceable gene editing in human hematopoietic stem and progenitor cells
- David Yudovich
- , Alexandra Bäckström
- & Jonas Larsson
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| Open AccessDevelopment of a pVEC peptide-based ribonucleoprotein (RNP) delivery system for genome editing using CRISPR/Cas9 in Chlamydomonas reinhardtii
- Seongsu Kang
- , Seungjib Jeon
- & Yeu-Chun Kim
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| Open AccessRedesigned reporter gene for improved proton exchange-based molecular MRI contrast
- Or Perlman
- , Hirotaka Ito
- & Christian T. Farrar
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| Open AccessFemale-to-male sex conversion in Ceratitis capitata by CRISPR/Cas9 HDR-induced point mutations in the sex determination gene transformer-2
- Roswitha A. Aumann
- , Irina Häcker
- & Marc F. Schetelig
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| Open AccessElectroporation and genetic supply of Cas9 increase the generation efficiency of CRISPR/Cas9 knock-in alleles in C57BL/6J mouse zygotes
- Samy Alghadban
- , Amine Bouchareb
- & Benjamin Davies
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| Open AccessGenome targeting by hybrid Flp-TAL recombinases
- Eugenia Voziyanova
- , Feng Li
- & Yuri Voziyanov
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| Open AccessBacteria-induced expression of the pig-derived protegrin-1 transgene specifically in the respiratory tract of mice enhances resistance to airway bacterial infection
- Fang Zeng
- , Chengcheng Zhao
- & Zhenfang Wu
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| Open AccessVisualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST
- Yankel Chekli
- , Caroline Peron-Cane
- & Christophe Beloin
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| Open AccessThe zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish
- Aubrey Converse
- & Peter Thomas
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| Open AccessBoundary sequences flanking the mouse tyrosinase locus ensure faithful pattern of gene expression
- Davide Seruggia
- , Almudena Fernández
- & Lluis Montoliu
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| Open AccessAdeno-associated virus-mediated gene delivery promotes S-phase entry-independent precise targeted integration in cardiomyocytes
- Yasuaki Kohama
- , Shuichiro Higo
- & Yasushi Sakata
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| Open AccessRapid, precise quantification of large DNA excisions and inversions by ddPCR
- Hannah L. Watry
- , Carissa M. Feliciano
- & Luke M. Judge