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| Open AccessSynthetic receptor scaffolds significantly affect the efficiency of cell fate signals
- Kirato Umene
- & Masahiro Kawahara
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| Open AccessGeneration of Fel d 1 chain 2 genome-edited cats by CRISPR-Cas9 system
- Sang Ryeul Lee
- , Kyung-Lim Lee
- & Il-Keun Kong
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| Open AccessCRISPR-mediated germline mutagenesis for genetic sterilization of Anopheles gambiae males
- Andrea L. Smidler
- , Eryney Marrogi
- & Flaminia Catteruccia
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| Open AccessCol1α2-Cre-mediated recombination occurs in various cell types due to Cre expression in epiblasts
- Yuzuru Matsumoto
- , Shinya Ikeda
- & Noboru Ashida
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| Open AccessEfficient gene editing in induced pluripotent stem cells enabled by an inducible adenine base editor with tunable expression
- Krittika Nandy
- , Dinesh Babu
- & Shaji R. Velayudhan
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Article
| Open AccessAePUb promoter length modulates gene expression in Aedes aegypti
- Michelle A. E. Anderson
- , Philip T. Leftwich
- & Luke Alphey
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| Open AccessNext-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata
- Serafima Davydova
- , Junru Liu
- & Angela Meccariello
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| Open AccessGenomic disturbance of vitellogenin 2 (vtg2) leads to vitellin membrane deficiencies and significant mortalities at early stages of embryonic development in zebrafish (Danio rerio)
- Ozlem Yilmaz
- , Emmanuelle Com
- & Julien Bobe
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| Open AccessPhenotypic screening of signaling motifs that efficiently induce cell proliferation
- Kirato Umene
- , Teruyuki Nagamune
- & Masahiro Kawahara
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| Open AccessSynthetic circuits based on split Cas9 to detect cellular events
- Alicja Przybyszewska-Podstawka
- , Jakub Czapiński
- & Adolfo Rivero-Müller
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| Open AccessChitinase of Trichoderma longibrachiatum for control of Aphis gossypii in cotton plants
- Waheed Anwar
- , Huma Amin
- & Karamat Ali Zohaib
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| Open AccessRemoval of evolutionarily conserved functional MYC domains in a tilapia cell line using a vector-based CRISPR/Cas9 system
- Chanhee Kim
- , Avner Cnaani
- & Dietmar Kültz
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| Open AccessCRISPR/Cas9-mediated disruption of CjACOS5 confers no-pollen formation on sugi trees (Cryptomeria japonica D. Don)
- Mitsuru Nishiguchi
- , Norihiro Futamura
- & Tsuyoshi Emilio Maruyama
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| Open AccessLong-read sequence analysis of MMEJ-mediated CRISPR genome editing reveals complex on-target vector insertions that may escape standard PCR-based quality control
- Yuki Higashitani
- & Kyoji Horie
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| Open AccessMolecular dissection and testing of PRSS37 function through LC–MS/MS and the generation of a PRSS37 humanized mouse model
- Courtney Sutton
- , Kaori Nozawa
- & Martin M. Matzuk
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| Open AccessPhenotypic consequences of a nanophthalmos-associated TMEM98 variant in human and mouse
- Mark M. Hassall
- , Shari Javadiyan
- & Owen M. Siggs
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Article
| Open AccessOptimized metrics for orthogonal combinatorial CRISPR screens
- Ronay Cetin
- , Martin Wegner
- & Manuel Kaulich
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| Open AccessTransgene-induced cell death following dengue-2 virus infection in Aedes aegypti
- Danilo O. Carvalho
- , Andre L. Costa-da-Silva
- & Margareth L. Capurro
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Article
| Open AccessSafety assessment of subchronic feeding of insect-resistant and herbicide-resistant transgenic soybeans to juvenile channel catfish (Ictalurus punctatus)
- Dan Xiang
- , Mingzhong Luo
- & Junfeng Xu
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| Open AccessCloning, characterization, and heterologous expression of a candidate Hirudin gene from the salivary gland transcriptome of Hirudo nipponia
- Ping Shi
- , Jian Wei
- & Zenghui Lu
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| Open AccessFull-length human dystrophin on human artificial chromosome compensates for mouse dystrophin deficiency in a Duchenne muscular dystrophy mouse model
- Yosuke Hiramuki
- , Satoshi Abe
- & Yasuhiro Kazuki
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| Open AccessCRISPR/Cas9 mediated specific ablation of vegfa in retinal pigment epithelium efficiently regresses choroidal neovascularization
- Jinkyu Park
- , Gang Cui
- & Suk Ho Byeon
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| Open AccessThe PROTECTOR strategy employs dCas orthologs to sterically shield off-target sites from CRISPR/Cas activity
- Daniel M. Sapozhnikov
- & Moshe Szyf
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| Open AccessRapid clonal identification of biallelic CRISPR/Cas9 knock-ins using SNEAK PEEC
- Sameer Singh
- , Anoosha Banerjee
- & Sebastian Klinge
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| Open AccessImproved selection of zebrafish CRISPR editing by early next-generation sequencing based genotyping
- Ewa Sieliwonczyk
- , Bert Vandendriessche
- & Dorien Schepers
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| Open AccessCRISPR-mediated generation and characterization of a Gaa homozygous c.1935C>A (p.D645E) Pompe disease knock-in mouse model recapitulating human infantile onset-Pompe disease
- Shih-hsin Kan
- , Jeffrey Y. Huang
- & Raymond Y. Wang
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| Open AccessEfficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice
- Manabu Ozawa
- , Jumpei Taguchi
- & Masahito Ikawa
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| Open AccessSTAT3 suppression and β-cell ablation enhance α-to-β reprogramming mediated by Pdx1
- Yuka Wakabayashi
- , Takeshi Miyatsuka
- & Hirotaka Watada
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| Open AccessGenetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes
- Marcela Nouzova
- , Marten J. Edwards
- & Fernando G. Noriega
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| Open AccessSuppressing gain-of-function proteins via CRISPR/Cas9 system in SCA1 cells
- Mariangela Pappadà
- , Ottavia Bonuccelli
- & Peggy Marconi
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| Open AccessGeneration of bright autobioluminescent bacteria by chromosomal integration of the improved lux operon ilux2
- Carola Gregor
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| Open AccessA growth-based platform for detecting domain–peptide interactions in the cytoplasm of mammalian cells
- Yosuke Kimura
- , Daiki Kashima
- & Masahiro Kawahara
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Article
| Open AccessEfficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model
- Marisa Carbonaro
- , Jeffrey Lee
- & Zhe Li
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| Open AccessEfficient genome editing in wild strains of mice using the i-GONAD method
- Yuji Imai
- , Akira Tanave
- & Tsuyoshi Koide
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Article
| Open AccessCRISPR-Cas12a nucleases function with structurally engineered crRNAs: SynThetic trAcrRNA
- D. J. Jedrzejczyk
- , L. D. Poulsen
- & R. T. Gill
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| Open AccessSmooth muscle protein 22α-Cre recombination in resting cardiac fibroblasts and hematopoietic precursors
- Shinya Ikeda
- , Sachiko Sugioka
- & Noboru Ashida
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Article
| Open AccessInsights from one thousand cloned dogs
- P. Olof Olsson
- , Yeon Woo Jeong
- & Woo Suk Hwang
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| Open AccessHideRNAs protect against CRISPR-Cas9 re-cutting after successful single base-pair gene editing
- Tim J. W. Harmsen
- , Colin E. J. Pritchard
- & Hein te Riele
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| Open AccessOxidative stress monitoring in iPSC-derived motor neurons using genetically encoded biosensors of H2O2
- Elizaveta Ustyantseva
- , Sophia V. Pavlova
- & Sergey P. Medvedev
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| Open AccessEvaluation of the α-casein (CSN1S1) locus as a potential target for a site-specific transgene integration
- A. V. Smirnov
- , G. V. Kontsevaya
- & N. R. Battulin
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| Open AccessHigh throughput screen for the improvement of inducible promoters for tumor microenvironment cues
- Omri Sharabi
- , Yariv Greenshpan
- & Roi Gazit
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| Open AccessPhosphate-regulated expression of the SARS-CoV-2 receptor-binding domain in the diatom Phaeodactylum tricornutum for pandemic diagnostics
- Samuel S. Slattery
- , Daniel J. Giguere
- & David R. Edgell
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Article
| Open AccessSingle 3′-exonuclease-based multifragment DNA assembly method (SENAX)
- Viet Linh Dao
- , Sheena Chan
- & Chueh Loo Poh
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Article
| Open AccessConstruction and validation of safe Clostridium botulinum Group II surrogate strain producing inactive botulinum neurotoxin type E toxoid
- Maria B. Nowakowska
- , Katja Selby
- & Miia Lindström
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| Open AccessMachine learning-based approaches for identifying human blood cells harboring CRISPR-mediated fetal chromatin domain ablations
- Yi Li
- , Shadi Zaheri
- & Leonidas Bleris
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| Open AccessGFAP splice variants fine-tune glioma cell invasion and tumour dynamics by modulating migration persistence
- Rebeca Uceda-Castro
- , Jessy V. van Asperen
- & Elly M. Hol
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| Open AccessShort term but highly efficient Cas9 expression mediated by excisional system using adenovirus vector and Cre
- Sayaka Nagamoto
- , Miyuki Agawa
- & Yumi Kanegae
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| Open AccessGeneration of mutant pigs by lipofection-mediated genome editing in embryos
- Maki Hirata
- , Manita Wittayarat
- & Takeshige Otoi
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| Open AccessOrphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish
- Nadja R. Brun
- , Matthew C. Salanga
- & John J. Stegeman