Browse our February issue

Latest Research

  • Article |

    2′-O-methylation within mRNA coding regions sterically perturbs interactions of ribosomal-monitoring bases with cognate codon–anticodon helices, leading to excessive rejection of cognate aminoacylated tRNAs during initial selection and proofreading.

    • Junhong Choi
    • , Gabriele Indrisiunaite
    • , Hasan DeMirci
    • , Ka-Weng Ieong
    • , Jinfan Wang
    • , Alexey Petrov
    • , Arjun Prabhakar
    • , Gideon Rechavi
    • , Dan Dominissini
    • , Chuan He
    • , Måns Ehrenberg
    •  & Joseph D. Puglisi
  • Article |

    In vivo, in vitro and in silico experiments demonstrate that interface residues of homomeric proteins are enriched toward protein C termini to avoid premature assembly and aggregation.

    • Eviatar Natan
    • , Tamaki Endoh
    • , Liora Haim-Vilmovsky
    • , Tilman Flock
    • , Guilhem Chalancon
    • , Jonathan T. S. Hopper
    • , Bálint Kintses
    • , Peter Horvath
    • , Lejla Daruka
    • , Gergely Fekete
    • , Csaba Pál
    • , Balázs Papp
    • , Erika Oszi
    • , Zoltán Magyar
    • , Joseph A. Marsh
    • , Adrian H. Elcock
    • , M. Madan Babu
    • , Carol V. Robinson
    • , Naoki Sugimoto
    •  & Sarah A. Teichmann
  • Technical Report |

    Yeast surface display platform allows nanobody discovery within two to three weeks. Examples include nanobodies for crystallographic applications, targeting nonpurified antigen or conformationally selective nanobodies to two distinct human GPCRs.

    • Conor McMahon
    • , Alexander S. Baier
    • , Roberta Pascolutti
    • , Marcin Wegrecki
    • , Sanduo Zheng
    • , Janice X. Ong
    • , Sarah C. Erlandson
    • , Daniel Hilger
    • , Søren G. F. Rasmussen
    • , Aaron M. Ring
    • , Aashish Manglik
    •  & Andrew C. Kruse
  • Article |

    Cryo-ET analyses of the microtubule-bound mouse dynein–dynactin complex reveal two dimeric dyneins interacting with the dynactin–cargo adaptor complex, a configuration that can account for processivity and directionality of dynein transport activity.

    • Danielle A. Grotjahn
    • , Saikat Chowdhury
    • , Yiru Xu
    • , Richard J. McKenney
    • , Trina A. Schroer
    •  & Gabriel C. Lander

News & Comment

  • News & Views |

    Chd1 is a highly conserved chromatin remodeler found across all eukaryotic species. A recent study shows the structural changes that take place when yeast Chd1 binds to its nucleosomal substrate and reveals how they relate to remodeler function.

    • Michaela M. Smolle
  • Editorial |

    This January 2018 issue starts the 25th year of NSMB’s journey. We mark the occasion by launching a special series that celebrates the exciting research uncovering the fundamental principles behind biological processes.

  • News & Views |

    The helicase intrinsic to DNA polymerase θ (Polθ), the versatile mediator of microhomology-based repair of DNA double-strand breaks and stalled replication forks, is now revealed to be a member of an elite group of proteins known as annealing helicases. This small family of enzymes remodels DNA intermediates in multiple repair processes that are crucial to preserving genome stability and warding off cancer and aging.

    • Judith L Campbell
    •  & Hongzhi Li
  • News & Views |

    The crystal structure of an oligosaccharyltransferase in complex with a sugar donor and an acceptor peptide provides insight into the mechanism of protein glycosylation and reveals how lipid-linked oligosaccharides are positioned in the enzyme active site.

    • Shiteshu Shrimal
    • , Natalia A Cherepanova
    •  & Reid Gilmore
  • News & Views |

    Deadenylation of mRNAs is generally associated with translational inhibition and mRNA decay. A study now reports that, unexpectedly, highly expressed genes tend to have shorter poly(A) tails and suggests that poly(A) tails can be 'pruned', generating a 30-nucleotide-biased phased distribution, likely due to protection by poly(A)-binding proteins.

    • Luciana A Castellano
    •  & Ariel A Bazzini
  • News & Views |

    Assembly of the small ribosomal subunit from an RNA strand and 33 proteins is an intricate and dynamic process. Two cryo-EM studies now provide insight into a complicated complex of at least 51 trans-factors that act on the preribosomal small subunit to sequentially fold it into a 3D molecular machine.

    • Joanna Rorbach
    • , Shintaro Aibara
    •  & Alexey Amunts

Series

NSMB 25

We mark the 25th year of NSMB with a special Series of commissioned Reviews and Comments that celebrate exciting research uncovering the fundamental principles behind biological processes.