Single-molecule localization microscopy

SMLM describes a family of imaging techniques with increased spatial resolution, able to find individual fluorescent molecules. 


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  • In this Primer, Flitsch and colleagues describe how biocatalysis is facilitating synthetic chemistry in both academia and industry. Detailed considerations required to find, select and optimize a biocatalyst are described, followed by an analysis of the performance metrics used to define a good industrial catalyst.

    • Elizabeth L. Bell
    • William Finnigan
    • Sabine L. Flitsch
  • This Primer provides an overview of the best practices for C–H activation as well as key advances in asymmetric, photoinduced and electrocatalytic-mediated catalysis for this synthetic platform. An overview of how C–H activation facilitates the synthesis of molecules such as structurally complex (bio)polymers and drugs is provided along with the current challenges and priorities for the next decade.

    • Torben Rogge
    • Nikolaos Kaplaneris
    • Lutz Ackermann
  • This Primer on electrochemical impedance spectroscopy (EIS) provides an experimental design guide to measure impedance and how these data are analysed. The range of applications that require EIS, from measuring battery performance to electrochemical biosensors, is highlighted. Limitations of the method along with emerging trends in experimental optimizations and data interpretation are also described.

    • Shangshang Wang
    • Jianbo Zhang
    • Mark E. Orazem
  • This Primer explains the central concepts of single-molecule localization microscopy (SMLM) before discussing experimental considerations regarding fluorophores, optics and data acquisition, processing and analysis. The Primer further describes recent high-impact discoveries made by SMLM techniques and concludes by discussing emerging methodologies.

    • Mickaël Lelek
    • Melina T. Gyparaki
    • Christophe Zimmer
  • Puzzarini and colleagues explore the computational characterization of medium-sized molecular systems using different spectroscopic techniques. The Primer provides essential information about the characteristics, accuracy and limitations of current computational approaches used for modelling spectroscopic phenomena with a focus on estimating error bars, limitations and coupling interpretability to accuracy.

    • Vincenzo Barone
    • Silvia Alessandrini
    • Cristina Puzzarini
  • Bian et al. discuss the utility of different variants of scanning probe microscopy, with a focus on scanning tunnelling microscopy and atomic force microscopy. They summarize how the tools are used in the life and physical sciences.

    • Ke Bian
    • Christoph Gerber
    • Ying Jiang
  • As Nature Reviews Methods Primers publishes its first articles, the editors outline the journal’s aims and scope and our contribution to the pursuit of reproducibility.