New nanomaterials are being developed for efficient biomolecule delivery to plants. However, detection and quantification of plant cell entry are challenging and currently rely on subjective methods that lack proper controls. The necessary considerations of performing nanoparticle-mediated delivery in plants and how to accurately quantify delivery efficiency are discussed.
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References
Rustgi, S., Naveed, S., Windham, J., Zhang, H. & Demirer, G. S. Plant biomacromolecule delivery methods in the 21st century. Front. Genome Ed. 4, 1011934 (2022).
Demirer, G. S. et al. Nanotechnology to advance CRISPR–Cas genetic engineering of plants. Nat. Nanotechnol. https://doi.org/10.1038/s41565-021-00854-y (2021).
Wang, J. W. et al. Nanoparticle-mediated genetic engineering of plants. Mol. Plant 12, 1037–1040 (2019).
Demirer, G. S. et al. High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants. Nat. Nanotechnol. 14, 456–464 (2019).
Demirer, G. S. et al. Carbon nanocarriers deliver siRNA to intact plant cells for efficient gene knockdown. Sci. Adv. 6, eaaz0495 (2020).
Zhang, H. et al. DNA nanostructures coordinate gene silencing in mature plants. Proc. Natl Acad. Sci. USA 116, 7543–7548 (2019).
Ng, K. K. et al. Intracellular delivery of proteins via fusion peptides in intact plants. PLoS ONE 11, e0154081 (2016).
Lee, S.-Y., Tyler, J. Y., Kim, S., Park, K. & Cheng, J.-X. FRET imaging reveals different cellular entry routes of self-assembled and disulfide bonded polymeric micelles. Mol. Pharm. 10, 3497–3506 (2013).
Wang, J. W., Goh, N., Lien, E., González-Grandio, E. & Landry, M. P. Quantification of cell penetrating peptide mediated delivery of proteins in plant leaves. Preprint at bioRxiv https://doi.org/10.1101/2022.05.03.490515 (2022).
Nekrasov, V., Staskawicz, B., Weigel, D., Jones, J. D. G. & Kamoun, S. Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nat. Biotechnol. 31, 691–693 (2013).
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The author thanks the Resnick Sustainability Institute at Caltech and the Henry Luce Foundation for funding.
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Demirer, G.S. Detecting and quantifying nanoparticle-mediated biomolecule delivery in plants. Nat Rev Methods Primers 3, 16 (2023). https://doi.org/10.1038/s43586-023-00213-2
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DOI: https://doi.org/10.1038/s43586-023-00213-2
