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Best practices for characterizing RNA lipid nanoparticles

To improve early-stage research in the field of RNA lipid nanoparticles, there are several best practices to be considered for the collection, interpretation and reporting of characterization data.

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Fig. 1: Properties that impact LNP characterization.

References

  1. Semple, S. C. et al. Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: formation of novel small multilamellar vesicle structures. Biochim. Biophys. Acta Biomembr. 1510, 152–166 (2001).

    Article  ADS  Google Scholar 

  2. Akinc, A. et al. The Onpattro story and the clinical translation of nanomedicines containing nucleic acid-based drugs. Nat. Nanotechnol. 14, 1084–1087 (2019).

    Article  ADS  Google Scholar 

  3. Kaplonek, P. et al. mRNA-1273 and BNT162b2 COVID-19 vaccines elicit antibodies with differences in Fc-mediated effector functions. Sci. Transl Med. 14, eabm2311 (2022).

    Article  Google Scholar 

  4. Lau, Y. M. A., Pang, J., Tilstra, G., Couture-Senécal, J. & Khan, O. F. The engineering challenges and opportunities when designing potent ionizable materials for the delivery of ribonucleic acids. Expert Opin. Drug Deliv. 19, 1650–1663 (2022).

    Article  Google Scholar 

  5. Sych, T. et al. High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01825-5 (2023).

    Article  Google Scholar 

  6. Tilstra, G. et al. Iterative design of ionizable lipids for intramuscular mRNA delivery. J. Am. Chem. Soc. 145, 2294–2304 (2023).

    Article  Google Scholar 

  7. Hassett, K. J. et al. Optimization of lipid nanoparticles for intramuscular administration of mRNA vaccines. Mol. Ther. Nucleic Acids 15, 1–11 (2019).

    Article  Google Scholar 

  8. Jayaraman, M. et al. Maximizing the potency of siRNA lipid nanoparticles for hepatic gene silencing in vivo. Angew. Chem. Int. Ed. 51, 8529–8533 (2012).

    Article  Google Scholar 

  9. Carrasco, M. J. et al. Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration. Commun. Biol. 4, 956 (2021).

    Article  Google Scholar 

Download references

Acknowledgements

The author thanks the members of the O.F.K. lab and the University of Toronto’s Medicine by Design initiative, which receives funding from the Canada First Research Excellence Fund (CFREF-2020). The author also thanks the New Frontiers in Research – Exploration stream (NFRFE-2022-00691), the Canada Research Chairs Program (CRC-2020-00097), the Natural Sciences and Engineering Research Council of Canada (RGPIN-2021-03421), the Canadian Institutes of Health Research (PTT-184009), and the Canada Foundation for Innovation John R. Evans Leaders Fund/Ontario Research Fund (41323) for support.

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Correspondence to Omar F. Khan.

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Khan, O.F. Best practices for characterizing RNA lipid nanoparticles. Nat Rev Methods Primers 3, 65 (2023). https://doi.org/10.1038/s43586-023-00258-3

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