To improve early-stage research in the field of RNA lipid nanoparticles, there are several best practices to be considered for the collection, interpretation and reporting of characterization data.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
A careful look at lipid nanoparticle characterization: analysis of benchmark formulations for encapsulation of RNA cargo size gradient
Scientific Reports Open Access 29 January 2024
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 1 digital issues and online access to articles
$99.00 per year
only $99.00 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Semple, S. C. et al. Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: formation of novel small multilamellar vesicle structures. Biochim. Biophys. Acta Biomembr. 1510, 152–166 (2001).
Akinc, A. et al. The Onpattro story and the clinical translation of nanomedicines containing nucleic acid-based drugs. Nat. Nanotechnol. 14, 1084–1087 (2019).
Kaplonek, P. et al. mRNA-1273 and BNT162b2 COVID-19 vaccines elicit antibodies with differences in Fc-mediated effector functions. Sci. Transl Med. 14, eabm2311 (2022).
Lau, Y. M. A., Pang, J., Tilstra, G., Couture-Senécal, J. & Khan, O. F. The engineering challenges and opportunities when designing potent ionizable materials for the delivery of ribonucleic acids. Expert Opin. Drug Deliv. 19, 1650–1663 (2022).
Sych, T. et al. High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01825-5 (2023).
Tilstra, G. et al. Iterative design of ionizable lipids for intramuscular mRNA delivery. J. Am. Chem. Soc. 145, 2294–2304 (2023).
Hassett, K. J. et al. Optimization of lipid nanoparticles for intramuscular administration of mRNA vaccines. Mol. Ther. Nucleic Acids 15, 1–11 (2019).
Jayaraman, M. et al. Maximizing the potency of siRNA lipid nanoparticles for hepatic gene silencing in vivo. Angew. Chem. Int. Ed. 51, 8529–8533 (2012).
Carrasco, M. J. et al. Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration. Commun. Biol. 4, 956 (2021).
Acknowledgements
The author thanks the members of the O.F.K. lab and the University of Toronto’s Medicine by Design initiative, which receives funding from the Canada First Research Excellence Fund (CFREF-2020). The author also thanks the New Frontiers in Research – Exploration stream (NFRFE-2022-00691), the Canada Research Chairs Program (CRC-2020-00097), the Natural Sciences and Engineering Research Council of Canada (RGPIN-2021-03421), the Canadian Institutes of Health Research (PTT-184009), and the Canada Foundation for Innovation John R. Evans Leaders Fund/Ontario Research Fund (41323) for support.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The author declares no competing interests.
Rights and permissions
About this article
Cite this article
Khan, O.F. Best practices for characterizing RNA lipid nanoparticles. Nat Rev Methods Primers 3, 65 (2023). https://doi.org/10.1038/s43586-023-00258-3
Published:
DOI: https://doi.org/10.1038/s43586-023-00258-3