Volume 16 Issue 5, May 2021

This protocol describes how to implement in vivo snapshot photoacoustic topography through an ergodic relay. The authors provide instructions for instrument setup, alignment, calibration and wide-field imaging in mice.

This protocol describes how to fabricate graphene electronic tattoos (GETs) that offer unique electromechanical properties. The GETs can be used for a variety of applications, including wearables, personalized biosensors and human–computer interfaces.

This protocol describes how to use atomic force microscopy to measure the elastic modulus of soft 2D surfaces and cell-laden 3D hydrogels. We provide instructions for sample preparation, instrument calibration and data collection and analysis.

This protocol describes two peat-based plant growth systems for microbiome research in Arabidopsis. While both systems support microbe-free plants and input microbiota control, GnotoPots have advantages for throughput and FlowPots for versatility.

This protocol describes a two-phase assessment of senescence in cultured cells or clinical specimens by first identifying senescent cells using a combination of markers and then defining the senescent subtype by measuring pro-inflammatory activity.

Primary, nonmalignant, human GC B cells are purified and expanded in culture. Genetic manipulation and engraftment into mice enable the generation of genetically customized, synthetic models of human lymphoma.

This protocol recovers prokaryotic genomes from short-read metagenomic data. De novo assembly and binning are used to reconstruct metagenome-assembled genomes, which after quality assessment can provide insights into uncultured bacterial species.

Human iPSCs are differentiated into HSCs by culture with growth factors. They respond to fibrogenic stimuli, arising as a new source of HSC-like cells for in vitro modeling. Subsequent coculture with hepatocytes facilitates self-assembly into 3D hepatic spheroids.

This protocol details the fabrication and application of reusable electrochemical affinity-based biosensors and their integration with microfluidic chips. The sensors can be used for the detection of soluble biomarkers on organ-on-a-chip platforms.

This protocol describes how to prepare and image human fetuses using microfocus computed tomography, a less invasive imaging alternative to conventional autopsy.

This protocol outlines simple and reproducible methods for assessing the biofilm inhibition and eradication capacities of novel antibiofilm agents against adherent bacterial biofilms grown in 96-well microtiter plates.

This protocol describes an approach suitable for genome-wide profiling of transcription factors and other chromatin-associated proteins in low-input samples obtained from single blastocysts or cells.

The authors provide protocols for chromatin tracing (for direct 3D tracing of chromatin folding along individual chromosomes) and multiplexed imaging of nucleome architectures and RNAs in single cells of cell lines and mammalian tissue, respectively.

This protocol describes a detailed workflow for performing genome-wide analyses of strand-specific enrichment of chromatin-associated proteins or modified histones on replicating DNA in low numbers of mammalian cells.