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Phase contrast (left) and immunostained micrographs (right) of perfusable human engineered microvessels used to investigate vascular barrier function. Immunostaining for VE-cadherin (white) and nuclei (magenta) demonstrates the formation of endothelial cell–cell adherens junctions that regulate the permeability of the vessel in response to flow.
Mouse pluripotent stem cells are grown in vitro into embryoid bodies before transplantation into mice, where they further differentiate into an integumentary organ system with appendages, including hair follicles and sebaceous glands.
This protocol describes how to fabricate and apply silicon-based structures for optically controlled neuromodulation. The structures can be used for nongenetic neuronal excitation in cultured neurons, brain slices, and in vivo applications.
Individual animals are cultivated in custom microfabricated multiwell substrates. This protocol describes how to design, produce, and image the plates, as well as analyze the resulting behavioral data.
A new protocol for high-speed whole-brain fluorescence imaging using block-face serial microscopy tomography (FAST). The protocol contains procedures for hardware assembly, sample preparation, imaging and data processing.
18F labeling of non-activated arenes (e.g., 3-[18F]fluoro-5-[(pyridin-3-yl)ethynyl] benzonitrile ([18F]FPEB)) is an unmet need for PET imaging. This protocol uses a spirocyclic iodonium ylide method for one-step, regioselective radiofluorination.
The aim of this spectral standardization model is to expedite multicenter studies with large numbers of samples. The protocol covers sample preparation, acquisition of FTIR spectra, data preprocessing and model standardization.
This protocol describes optimized procedures for transcriptome-wide sequencing of native 5′ and 3′ ends of mRNAs captured from the same sample to assess the full diversity of processed transcripts that exist in human cells.
This protocol is used to detect RNA decay intermediates in human cultured cells. Reporter mRNAs can be detected and quantified with molecular biology (northern blot, qPCR) and single-molecule fluorescence microscopy (smFISH, live imaging) approaches.
Tubulin with controlled posttranslational modifications can be purified by cycles of polymerization and depolymerization from cultured cells or single mouse brains for use in in vitro reconstitution assays.