Neurostimulation protocols

Protocols

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  Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo

  This protocol describes the use of noninvasively triggered light in deep tissue via focused ultrasound-activated and systemically injected mechanoluminescent nanotransducers to achieve localized emissions with submillimeter resolution and millisecond response times.

  • Shan Jiang
  • Xiang Wu
  • Guosong Hong

  Protocol1 Nov 2023 Nature Protocols
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  All-optical interrogation of neural circuits in behaving mice

  This protocol describes all-optical interrogation experiments in awake, behaving mice, demonstrating the utility of this strategy in three brain areas—barrel cortex, visual cortex and hippocampus—by using different experimental setups.

  • Lloyd E. Russell
  • Henry W. P. Dalgleish
  • Michael Häusser

  Protocol27 Apr 2022 Nature Protocols
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  Engineering brain assembloids to interrogate human neural circuits

  A protocol is described for generating human brain assembloids and performing viral labeling and retrograde tracing, 3D live imaging of axon projection and optogenetics with calcium imaging and electrophysiological recordings to model neural circuits.

  • Yuki Miura
  • Min-Yin Li
  • Sergiu P. Pașca

  Protocol6 Jan 2022 Nature Protocols
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  Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience

  The authors present detailed procedures to fabricate, encapsulate and implant advanced wireless battery-free devices in head- and back-mounted forms in order to facilitate optogenetic experiments in freely moving rodents.

  • Yiyuan Yang
  • Mingzheng Wu
  • John A. Rogers

  Protocol16 Feb 2022 Nature Protocols
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  Imaging and optogenetic modulation of vascular mural cells in the live brain

  This protocol describes a set of tools and procedures for (1) intravital labeling for the identification of mural cells, (2) in vivo calcium imaging of pericytes and vSMCs, and (3) single- and two-photon optogenetics to study blood flow control.

  • Lei Tong
  • Robert A. Hill
  • Jaime Grutzendler

  Protocol9 Dec 2020 Nature Protocols
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  Imaging neuromodulators with high spatiotemporal resolution using genetically encoded indicators

  This protocol describes how to redesign, characterize, validate and apply genetically encoded dopamine sensors, such as those of the dLight1 family, to measure dopamine transients in cultured cells, neurons, acute brain slices and freely behaving, awake mice.

  • Tommaso Patriarchi
  • Jounhong Ryan Cho
  • Lin Tian

  Protocol15 Nov 2019 Nature Protocols
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  High-throughput multicolor optogenetics in microwell plates

  A protocol for the assembly and use of the optoPlate-96, a platform for high-throughput three-color optogenetics experiments in microwell plates. With the provided design files, users can assemble the optoPlate-96 from 3D-printed and laser-cut components.

  • Lukasz J. Bugaj
  • Wendell A. Lim

  Protocol24 Jun 2019 Nature Protocols
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  Nongenetic optical neuromodulation with silicon-based materials

  This protocol describes how to fabricate and apply silicon-based structures for optically controlled neuromodulation. The structures can be used for nongenetic neuronal excitation in cultured neurons, brain slices, and in vivo applications.

  • Yuanwen Jiang
  • Ramya Parameswaran
  • Bozhi Tian

  Protocol12 Apr 2019 Nature Protocols
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  Holographic two-photon activation for synthetic optogenetics

  This protocol describes how to make two-photon excitable genetically modified glutamate receptors for in vivo optogenetics.

  • Ido Carmi
  • Marco De Battista
  • Shai Berlin

  Protocol25 Feb 2019 Nature Protocols
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  Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors

  This protocol describes how to combine up to four genetically encoded fluorescent sensors to image redox landscapes. The procedure describes applications in live imaging and flow cytometry of cultured cells, and in vivo imaging in zebrafish larvae.

  • Yejun Zou
  • Aoxue Wang
  • Yuzheng Zhao

  Protocol26 Sep 2018 Nature Protocols
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  Closed-loop optogenetic intervention in mice

  • Caren Armstrong
  • Esther Krook-Magnuson
  • Ivan Soltesz

  Protocol11 Jul 2013 Nature Protocols
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  Targeted in vivo genetic manipulation of the mouse or rat brain by in utero electroporation with a triple-electrode probe

  This protocol extension describes how to electroporate rodent neuronal progenitors in utero with greater accuracy by using triple-electrode probes.

  • Joanna Szczurkowska
  • Andrzej W Cwetsch
  • Laura Cancedda

  Protocol Extension4 Feb 2016 Nature Protocols