Volume 13 Issue 1, January 2018

This protocol describes a microfluidics approach for culturing liquid-biopsy-derived circulating tumor cell clusters to predict a patient's response toward various therapeutic strategies.

This protocol describes a method for cataloging genome-wide mutations that accumulated during life or culture in single adult stem cells of different human tissues, by combining whole-genome sequencing with organoid-culture technologies.

The applications of solution-state NMR of membrane proteins are often limited by difficulty in finding a suitable membrane mimetic of tailored size that shows native-like membrane properties and provides long-term stability. This protocol describes how to assemble phospholipid nanodiscs and incorporate membrane proteins for NMR-structural studies.

Mammalian cells are powerful expression systems for producing glycosylated recombinant antibody preparations with minimal endotoxin contamination. This protocol describes procedures for antibody design, expression, purification and characterization.

This protocol describes a dual mRNA and protein labeling strategy that allows identification of activated neuronal assemblies in response to two temporally separated stimuli in mouse brain sections.

3D molecular cartography is used for mapping of metabolites in our environment. This protocol describes the procedures for sample collection and processing, mass spectrometry analysis, and data processing and visualization.

This protocol describes how to design and use fluorescent translocation reporters for live measurements of kinase activity in single cells.

This protocol describes a microfluidics platform, termed 'MACS', for single-cell microscopy of suspension cells. MACS temporarily immobilizes the cells, allowing for high-throughput and high-resolution imaging, cell sorting, and single-molecule detection.

This protocol describes Easi-CRISPR, a method for creating knock-in, conditional knockout, and knockdown mouse models by CRISPR/Cas9-based genome engineering using long single-stranded DNA donor templates.