Volume 17 Issue 3, March 2015

Volume 17 Issue 3

Lysosomal calcium release stimulates calcineurin, which dephosphorylates and activates TFEB to control autophagy.

[article p288]

Editorials

  • Editorial |

    In this issue, we present the first of a series of Reviews discussing different aspects of cell metabolism.

Reviews

News and Views

  • News & Views |

    Cells often migrate in tightly connected groups with coordinated movement and polarity. The collective migration of epithelial cell sheets is now shown to be mediated by a signalling axis that involves the merlin tumour-suppressor protein, the tight-junction-associated angiomotin–Rich1 complex and the Rac1 small GTPase.

    • Ansgar Zoch
    •  & Helen Morrison
  • News & Views |

    Selective autophagy is essential for maintaining cellular homeostasis under different growth conditions. Huntingtin, mutated versions of which have been implicated in Huntington disease, is now shown to act as a scaffold protein that couples the induction of autophagy and the selective recruitment of cargo into autophagosomes.

    • Amir Gelman
    • , Moran Rawet-Slobodkin
    •  & Zvulun Elazar

Articles

  • Article |

    Westlake and colleagues discover that membrane shaping EHD proteins participate in ciliogenesis by taking part in ciliary vesicle formation and transition zone protein recruitment.

    • Quanlong Lu
    • , Christine Insinna
    • , Carolyn Ott
    • , Jimmy Stauffer
    • , Petra A. Pintado
    • , Juliati Rahajeng
    • , Ulrich Baxa
    • , Vijay Walia
    • , Adrian Cuenca
    • , Yoo-Seok Hwang
    • , Ira O. Daar
    • , Susana Lopes
    • , Jennifer Lippincott-Schwartz
    • , Peter K. Jackson
    • , Steve Caplan
    •  & Christopher J. Westlake
  • Article |

    Bagnat, Martín-Belmonte and colleagues reveal that plasmolipin (PLLP) is upregulated in the zebrafish midgut during development and controls epithelial patterning by promoting polarized endocytosis.

    • Alejo E. Rodríguez-Fraticelli
    • , Jennifer Bagwell
    • , Minerva Bosch-Fortea
    • , Gaelle Boncompain
    • , Natalia Reglero-Real
    • , Maria J. García-León
    • , Germán Andrés
    • , Maria L. Toribio
    • , Miguel A. Alonso
    • , Jaime Millán
    • , Franck Perez
    • , Michel Bagnat
    •  & Fernando Martín-Belmonte
  • Article |

    Zhang, Cuervo and colleagues find that Huntingtin (Htt), which is commonly mutated in Huntington disease, regulates selective autophagy. Htt enhances interactions between p62, LC3 and ubiquitylated cargo and derepresses ULK1 kinase activity.

    • Yan-Ning Rui
    • , Zhen Xu
    • , Bindi Patel
    • , Zhihua Chen
    • , Dongsheng Chen
    • , Antonio Tito
    • , Gabriela David
    • , Yamin Sun
    • , Erin F. Stimming
    • , Hugo J. Bellen
    • , Ana Maria Cuervo
    •  & Sheng Zhang
  • Article |

    Spatz and colleagues report that intercellular pulling forces between leader and follower cells in migrating epithelial sheets regulate merlin subcellular localization and the crosstalk between merlin and Rac1 to promote collective cell migration.

    • Tamal Das
    • , Kai Safferling
    • , Sebastian Rausch
    • , Niels Grabe
    • , Heike Boehm
    •  & Joachim P. Spatz
  • Article |

    Medina, Ballabio and colleagues report that calcium release from the lysosome stimulates calcineurin, which dephosphorylates and activates TFEB. These findings reveal a central role for calcium signalling in autophagy and lysosome homeostasis.

    • Diego L. Medina
    • , Simone Di Paola
    • , Ivana Peluso
    • , Andrea Armani
    • , Diego De Stefani
    • , Rossella Venditti
    • , Sandro Montefusco
    • , Anna Scotto-Rosato
    • , Carolina Prezioso
    • , Alison Forrester
    • , Carmine Settembre
    • , Wuyang Wang
    • , Qiong Gao
    • , Haoxing Xu
    • , Marco Sandri
    • , Rosario Rizzuto
    • , Maria Antonietta De Matteis
    •  & Andrea Ballabio
  • Article |

    Bar-Eli and colleagues report that CREB-mediated downregulation of the RNA-editing enzyme ADAR1 results in decreased adenosine-to-inosine editing of miR-455-5p, which enhances melanoma growth and metastasis.

    • Einav Shoshan
    • , Aaron K. Mobley
    • , Russell R. Braeuer
    • , Takafumi Kamiya
    • , Li Huang
    • , Mayra E. Vasquez
    • , Ahmad Salameh
    • , Ho Jeong Lee
    • , Sun Jin Kim
    • , Cristina Ivan
    • , Guermarie Velazquez-Torres
    • , Ka Ming Nip
    • , Kelsey Zhu
    • , Denise Brooks
    • , Steven J. M. Jones
    • , Inanc Birol
    • , Maribel Mosqueda
    • , Yu-ye Wen
    • , Agda Karina Eterovic
    • , Anil K. Sood
    • , Patrick Hwu
    • , Jeffrey E. Gershenwald
    • , A. Gordon Robertson
    • , George A. Calin
    • , Gal Markel
    • , Isaiah J. Fidler
    •  & Menashe Bar-Eli
  • Article |

    Aifantis and colleagues report that FBXW7α controls the heat-shock response pathway by targeting HSF1 for degradation. In melanoma FBXW7α deficiency leads to increased nuclear HSF1, and induction of a pro-invasive gene expression program.

    • Nikos Kourtis
    • , Rana S. Moubarak
    • , Beatriz Aranda-Orgilles
    • , Kevin Lui
    • , Iraz T. Aydin
    • , Thomas Trimarchi
    • , Farbod Darvishian
    • , Christine Salvaggio
    • , Judy Zhong
    • , Kamala Bhatt
    • , Emily I. Chen
    • , Julide T. Celebi
    • , Charalampos Lazaris
    • , Aristotelis Tsirigos
    • , Iman Osman
    • , Eva Hernando
    •  & Iannis Aifantis

Letter

Technical Report

  • Technical Report |

    Magness and colleagues present a microwell-based culture system to analyse interactions between intestinal stem cells (ISCs) and Paneth cells, and show that their direct contact enhances formation of ISC-derived organoids.

    • Adam D. Gracz
    • , Ian A. Williamson
    • , Kyle C. Roche
    • , Michael J. Johnston
    • , Fengchao Wang
    • , Yuli Wang
    • , Peter J. Attayek
    • , Joseph Balowski
    • , Xiao Fu Liu
    • , Ryan J. Laurenza
    • , Liam T. Gaynor
    • , Christopher E. Sims
    • , Joseph A. Galanko
    • , Linheng Li
    • , Nancy L. Allbritton
    •  & Scott T. Magness

Corrigendum