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In this picture of live budding yeast cells, the fluorescence of green fluorescent protein (GFP) fused to the DNA-replication factor Mcm4 has been merged with a phase-contrast image. Mcm4–GFP accumulates in the nucleus at the end of mitosis, and is subsequently excluded from the nucleus by cyclindependent kinase activity, as bud emergence and DNA replication occur in the next cell cycle. [Cover design: Majo Xeridat] [article, p. 415; news and views, p.E175]