Organoid protocols

Organoid culture systems are self-renewing 3D models derived from stem cells (pluripotent or adult-derived) or cancer cells that recapitulate characteristics of their tissues of origin. Here, we provide protocols for growing and studying various types of organoid (brain, skin, kidney, liver, bile duct, prostate, placenta, breast, colon, stomach, pancreas, lung, heart, and blood vessel) generated from both healthy and cancer cells.

Whole-mount 3D confocal image of a human colonic organoid immunolabeled for F-actin (red–yellow) and E-cadherin (E-cad; blue) (fructose–glycerol clearing; 25× oil objective).