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Open Access
Featured
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Article
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Real-time detection of 20 amino acids and discrimination of pathologically relevant peptides with functionalized nanopore
A copper(II)-functionalized Mycobacterium smegmatis porin A nanopore enables direct identification of all 20 proteinogenic amino acids, one unnatural amino acid and two post-translational modifications, and shows potential for peptide discrimination and sequencing.
- Ming Zhang
- , Chao Tang
- & Jia Geng
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Method to Watch |
Nanopores for sequencing proteins
Developments in nanopore-based peptide detection and sequencing show promise of a breakthrough.
- Arunima Singh
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Article |
Peptide sequencing based on host–guest interaction-assisted nanopore sensing
A phenylalanine-containing peptide probe can be used for discriminating all 20 amino acids via current blockage during translocation through an α-hemolysin (αHL) nanopore. The paper provides proof-of-concept peptide sequencing demonstrations.
- Yun Zhang
- , Yakun Yi
- & Hai-Chen Wu
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Article |
Adaptable, turn-on maturation (ATOM) fluorescent biosensors for multiplexed detection in cells
Adaptable, turn-on maturation (ATOM) biosensors use monobody or nanobody targeting to control fluorescent protein maturation for fluorescence in the presence of target biomolecules, enabling bright and specific cellular biosensing.
- Harsimranjit Sekhon
- , Jeung-Hoi Ha
- & Stewart N. Loh
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Research Highlight |
Capturing subcellular metal ion dynamics
Newly developed DNA devices can be used to study intracellular sodium and potassium ion levels with organellar resolution.
- Rita Strack
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Research Highlight |
Droplet-based power sources for modulating neural activity
Miniaturized power sources consisting of a few droplets can generate enough power to modulate neuronal activity.
- Nina Vogt
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Brief Communication |
Molecular mechanocytometry using tension-activated cell tagging
Tension-activated cell tagging (TaCT) is a new method that uses flow cytometry to sort mechanically active cells based on the forces generated by their surface adhesion receptors.
- Rong Ma
- , Sk Aysha Rashid
- & Khalid Salaita
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Article |
Unambiguous discrimination of all 20 proteinogenic amino acids and their modifications by nanopore
A Ni2+-modified MspA nanopore construct can unambiguously discriminate the 20 proteogenic amino acids as well as several post-translational modifications.
- Kefan Wang
- , Shanyu Zhang
- & Shuo Huang
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Brief Communication
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DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging
SABER-IMC combines DNA-based signal amplification by exchange reaction (SABER) with imaging mass cytometry (IMC) to enable simultaneous and highly multiplexed marker detection, even of low-abundance markers not detectable with IMC alone.
- Tsuyoshi Hosogane
- , Ruben Casanova
- & Bernd Bodenmiller
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Editorial |
Single-cell proteomics: challenges and prospects
Single-cell proteomics is a challenging goal and an area of rapid methods development. This Focus issue highlights the many paths toward high-throughput, high-sensitivity measurements.
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Article |
Geometric engineering of organoid culture for enhanced organogenesis in a dish
A scalable platform with geometrical reconfiguration of culture systems for long-term growth and maturation of organoids.
- Sunghee Estelle Park
- , Shawn Kang
- & Dan Dongeun Huh
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Comment |
Advances in nanopore direct RNA sequencing
Nanopore direct RNA sequencing (DRS) reads continuous native RNA strands. Early adopters have used this technology to document nucleotide modifications and 3′ polyadenosine tails on RNA strands without added chemistry steps. Individual strands ranging in length from 70 to 26,000 nucleotides have been sequenced. In our opinion, broader acceptance of nanopore DRS by molecular biologists and cell biologists will be accelerated by higher basecall accuracy and lower RNA input requirements.
- Miten Jain
- , Robin Abu-Shumays
- & Mark Akeson
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Research Highlight |
A closer look at FluoroCubes
Researchers explore the unique and puzzling photostability of DNA FluoroCubes. Moreover, they improve the probes’ performance and highlight their diverse applicability.
- Rita Strack
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Research Highlight |
Nanopores distinguish RNA modifications
Different nucleotides and derivatives can be read out by MspA-PBA, an engineered nanopore.
- Lin Tang
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Research Highlight |
Modeling multi-organ systems on a chip
To recapitulate some of the complexities of the human physiology, engineered tissues can be linked by vascular flow in a multi-organ chip.
- Nina Vogt
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This Month |
Vahid Sandoghdar
From a physicist who explores biology’s unknowns, greater precision by linking two methods.
- Vivien Marx
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Article
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Precision size and refractive index analysis of weakly scattering nanoparticles in polydispersions
iNTA combines interferometric detection of scattering with nanoparticle tracking analysis for determining the size and refractive index distributions of nanoparticles in suspension with high sensitivity and precision.
- Anna D. Kashkanova
- , Martin Blessing
- & Vahid Sandoghdar
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News & Views |
Expansion microscopy opens the door to exploring more challenges
Cryofixation-based ultrastructure-expansion microscopy (cryo-ExM) bypasses artifacts caused by chemical fixation and establishes more-native preservation of biological samples.
- Mengfei Gao
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Research Highlight |
Reading proteins with nanopores
Two studies use nanopores for single-protein fingerprinting and make headway toward single-protein sequencing.
- Rita Strack
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Article |
Monitoring protein conformational changes using fluorescent nanoantennas
Fluorescent nanoantennas represent a versatile detection strategy for monitoring fast, large- and small-scale protein dynamics.
- Scott G. Harroun
- , Dominic Lauzon
- & Alexis Vallée-Bélisle
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Research Highlight |
Tracking protein conformation in live cells
A simple ‘binder/tag’ approach allows tracking of individual protein conformation changes in live cells.
- Allison Doerr
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Research Highlight |
Nanopore-detectable reporter proteins
NanoporeTERs, engineered reporter proteins, can be detected on MinION nanopore sensor arrays.
- Arunima Singh
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Research Highlight |
Trapping virus in a shell
Researchers design DNA shells to trap virus particles and thus prevent interactions between viruses and host cells.
- Lei Tang
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Article |
Ultrasensitive ultrasound imaging of gene expression with signal unmixing
Acoustic reporter genes can be imaged with high sensitivity using the BURST imaging sequence, allowing the detection of single cells under optimal conditions.
- Daniel P. Sawyer
- , Avinoam Bar-Zion
- & Mikhail G. Shapiro
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Research Highlight |
High-resolution optical tweezers
Germanium nanoparticles enable optical trapping at high spatiotemporal resolution.
- Nina Vogt
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Article |
Mass spectrometry imaging of untreated wet cell membranes in solution using single-layer graphene
Application of a single layer of graphene to untreated wet mammalian cells enables mass spectrometry imaging of cellular membranes of live cells in solution at subcellular resolution.
- Heejin Lim
- , Sun Young Lee
- & Dae Won Moon
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Article |
Exosome detection via the ultrafast-isolation system: EXODUS
EXODUS is a high-speed isolation method for the enrichment of exosome from biological fluids with high purity and yield.
- Yuchao Chen
- , Qingfu Zhu
- & Fei Liu
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Review Article |
Advances and challenges for fluorescence nanothermometry
This Review on nanothermometry introduces the many types of nanothermometers and their cellular and in vivo applications, as well as best practices for accurate measurements.
- Jiajia Zhou
- , Blanca del Rosal
- & Dayong Jin
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Research Highlight |
A dual-constriction biological nanopore
Addition of a second constriction to CsgG-family nanopores improves the accuracy of homopolymer sequencing.
- Arunima Singh
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Article |
Genetically encoded tags for direct synthesis of EM-visible gold nanoparticles in cells
Genetically encoded cysteine-rich tags enable formation of gold nanoparticles in situ for single-molecule imaging of individual proteins in the context of cellular ultrastructure in bacterial, yeast and mammalian cells.
- Zhaodi Jiang
- , Xiumei Jin
- & Wanzhong He
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Research Highlight |
It’s a material world
The assembly of polymers can be genetically targeted to specific neurons or other cells to manipulate their properties.
- Nina Vogt
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Technology Feature |
May mechanobiology work forcefully for you
Mechanical measurements would be easy if cells were homogeneous objects — they’re not.
- Vivien Marx
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Research Highlight |
Non-invasive and fast control of neural activity
Chemomagnetics enable remote and non-invasive modulation of neural activity in behaving mice.
- Nina Vogt
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Research Highlight |
Coupling CRISPR to smart hydrogels
The CRISPR–Cas system has become a powerful tool in genome editing and now expands its footprint into materials science.
- Lei Tang
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Research Highlight |
A nanorotor for amplifying molecular movements
A DNA origami rotor amplifies and tracks DNA movements during biochemical reactions, uncovering action mechanisms of genome-processing enzymes.
- Lei Tang
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Perspective |
FRET as a biomolecular research tool — understanding its potential while avoiding pitfalls
Best practices for carrying out and reporting ensemble FRET measurements in the cuvette and in cells.
- W. Russ Algar
- , Niko Hildebrandt
- & Igor L. Medintz
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Brief Communication |
Thermophoretic trap for single amyloid fibril and protein aggregation studies
A thermophoretic trapping approach enables users to observe the behavior of single amyloid fibrils in solution for hours without any immobilization.
- Martin Fränzl
- , Tobias Thalheim
- & Frank Cichos
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Article |
SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues
Using primer-exchange reactions, SABER extends FISH probes with repetitive sequences that can accommodate multiple fluorescent imager strands, resulting in up to 450-fold signal amplification. SABER is showcased in DNA and RNA FISH experiments across a range of complex biological samples.
- Jocelyn Y. Kishi
- , Sylvain W. Lapan
- & Peng Yin
Molecular pixelation: spatial proteomics of single cells by sequencing
Nanopore-based detection of phosphorylation
Giant assembly of DNA-origami slats
Multiomics sequencing goes spatial