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| Open AccessEfficient introgression of allelic variants by embryo-mediated editing of the bovine genome
- Jingwei Wei
- , Stefan Wagner
- & Götz Laible
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| Open AccessTrans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes
- Eli J. Fine
- , Caleb M. Appleton
- & Gang Bao
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| Open AccessGONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice
- Gou Takahashi
- , Channabasavaiah B Gurumurthy
- & Masato Ohtsuka
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| Open AccessElectroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing
- Masakazu Hashimoto
- & Tatsuya Takemoto
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| Open AccessePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways
- J. Andrew Jones
- , Victoria R. Vernacchio
- & Mattheos A. G. Koffas
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| Open AccessRapGene: a fast and accurate strategy for synthetic gene assembly in Escherichia coli
- Massimiliano Zampini
- , Pauline Rees Stevens
- & Finbarr Hayes
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| Open AccessGeneration of mutant mice via the CRISPR/Cas9 system using FokI-dCas9
- Satoshi Hara
- , Moe Tamano
- & Shuji Takada
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Article
| Open AccessCell proliferation by silk gut incorporating FGF-2 protein microcrystals
- Eiji Kotani
- , Naoto Yamamoto
- & Hajime Mori
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| Open AccessA minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules
- Judit Espana-Agusti
- , David A. Tuveson
- & Athena Matakidou
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Article
| Open AccessCRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells
- Yasuyoshi Kimura
- , Masaaki Oda
- & Toru Nakano
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| Open AccessGene targeting by TALEN-induced homologous recombination in goats directs production of β-lactoglobulin-free, high-human lactoferrin milk
- Chenchen Cui
- , Yujie Song
- & Yong Zhang
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Article
| Open AccessMethod of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments
- Kenji Tsuge
- , Yukari Sato
- & Mitsuhiro Itaya
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Article
| Open AccessThe transgenic cloned pig population with integrated and controllable GH expression that has higher feed efficiency and meat production
- Huiming Ju
- , Jiaqing Zhang
- & Kui Li
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| Open AccessCRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells
- Michael Ratz
- , Ilaria Testa
- & Stefan Jakobs
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Article
| Open AccessProduction of diacetyl by metabolically engineered Enterobacter cloacae
- Lijie Zhang
- , Yingxin Zhang
- & Cuiqing Ma
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| Open AccessAn efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori
- Dingpei Long
- , Weijian Lu
- & Aichun Zhao
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| Open AccessSite-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway
- Jae Seong Lee
- , Thomas Beuchert Kallehauge
- & Helene Faustrup Kildegaard
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Article
| Open AccessCRISPR-engineered mosaicism rapidly reveals that loss of Kcnj13 function in mice mimics human disease phenotypes
- Hua Zhong
- , Yiyun Chen
- & Graeme Mardon
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| Open AccessEfficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA
- Yong Wang
- , Yinan Du
- & Hong Wei
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| Open AccessAngiotensin-converting enzyme 2/angiotensin-(1–7)/Mas axis prevents lipopolysaccharide–induced apoptosis of pulmonary microvascular endothelial cells by inhibiting JNK/NF–κB pathways
- Yingchuan Li
- , Yongmei Cao
- & Wei Jiang
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| Open AccessOptimized tuning of TALEN specificity using non-conventional RVDs
- Alexandre Juillerat
- , Coline Pessereau
- & Philippe Duchateau
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| Open AccessA controllable on-off strategy for the reproductive containment of fish
- Yunsheng Zhang
- , Ji Chen
- & Wei Hu
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| Open AccessAsymmetric parental genome engineering by Cas9 during mouse meiotic exit
- Toru Suzuki
- , Maki Asami
- & Anthony C. F. Perry
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| Open AccessEfficient bi-allelic gene knockout and site-specific knock-in mediated by TALENs in pigs
- Jing Yao
- , Jiaojiao Huang
- & Jianguo Zhao
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| Open AccessGenome editing of BmFib-H gene provides an empty Bombyx mori silk gland for a highly efficient bioreactor
- Sanyuan Ma
- , Run Shi
- & Qingyou Xia
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| Open AccessSimple knockout by electroporation of engineered endonucleases into intact rat embryos
- Takehito Kaneko
- , Tetsushi Sakuma
- & Tomoji Mashimo
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Article
| Open AccessProduction of recombinant human proinsulin in the milk of transgenic mice
- Xi Qian
- , Jana Kraft
- & Feng-Qi Zhao
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| Open AccessDown syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies
- Yasuhiro Kazuki
- , Yuwna Yakura
- & Mitsuo Oshimura
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| Open AccessGeneration of muscular dystrophy model rats with a CRISPR/Cas system
- Katsuyuki Nakamura
- , Wataru Fujii
- & Masugi Nishihara
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| Open AccessRapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system
- Masafumi Inui
- , Mami Miyado
- & Shuji Takada
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| Open AccessMultiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system
- Tetsushi Sakuma
- , Ayami Nishikawa
- & Takashi Yamamoto
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| Open AccessEfficient genetic manipulation of the NOD-Rag1-/-IL2RgammaC-null mouse by combining in vitro fertilization and CRISPR/Cas9 technology
- Feng Li
- , Dale O. Cowley
- & Lishan Su
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| Open AccessMulti-wavelength photoacoustic imaging of inducible tyrosinase reporter gene expression in xenograft tumors
- Robert J. Paproski
- , Andrew Heinmiller
- & Roger J. Zemp
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Article
| Open AccessA rapid and robust selection procedure for generating drug-selectable marker-free recombinant malaria parasites
- Giulia Manzoni
- , Sylvie Briquet
- & Olivier Silvie
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| Open AccessNon-integrating gamma-retroviral vectors as a versatile tool for transient zinc-finger nuclease delivery
- Sylwia Bobis-Wozowicz
- , Melanie Galla
- & Toni Cathomen
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| Open AccessCRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori
- Sanyuan Ma
- , Jiasong Chang
- & Qingyou Xia
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| Open AccessImproved site-specific recombinase-based method to produce selectable marker- and vector-backbone-free transgenic cells
- Yuan Yu
- , Qi Tong
- & Yong Zhang
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| Open AccessIdentification of germline transcriptional regulatory elements in Aedes aegypti
- Omar S. Akbari
- , Philippos A. Papathanos
- & Bruce A. Hay
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| Open AccessProducing aglycons of ginsenosides in bakers' yeast
- Zhubo Dai
- , Beibei Wang
- & Xueli Zhang
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| Open AccessA robust TALENs system for highly efficient mammalian genome editing
- Yuanxi Feng
- , Siliang Zhang
- & Xin Huang
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| Open AccessRepeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity
- Tetsushi Sakuma
- , Hiroshi Ochiai
- & Takashi Yamamoto
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| Open AccessGeneration of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA
- Daisuke Mashiko
- , Yoshitaka Fujihara
- & Masahito Ikawa
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| Open AccessCodon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris
- Ping Yu
- , Yuan Yan
- & Xiangyang Wang
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| Open AccessLive pigs produced from genome edited zygotes
- Simon G. Lillico
- , Chris Proudfoot
- & C. Bruce A. Whitelaw
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| Open AccessAntioxidant enzyme, 3-mercaptopyruvate sulfurtransferase-knockout mice exhibit increased anxiety-like behaviors: a model for human mercaptolactate-cysteine disulfiduria
- Noriyuki Nagahara
- , Masatoshi Nagano
- & Hidenori Suzuki
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| Open AccessProduction of biallelic CMP-Neu5Ac hydroxylase knock-out pigs
- Deug-Nam Kwon
- , Kiho Lee
- & Jin-Hoi Kim
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| Open AccessGenetic engineering of yellow betalain pigments beyond the species barrier
- Takashi Nakatsuka
- , Eri Yamada
- & Masahiro Nishihara
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| Open AccessImproved adeno-associated virus (AAV) serotype 1 and 5 vectors for gene therapy
- Dwaipayan Sen
- , Balaji Balakrishnan
- & Giridhara R. Jayandharan
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| Open AccessEfficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes
- Tomoji Mashimo
- , Takehito Kaneko
- & Tadao Serikawa