Featured
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Research Highlight |
It’s a material world
The assembly of polymers can be genetically targeted to specific neurons or other cells to manipulate their properties.
- Nina Vogt
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Editorial |
All methods on deck
More basic research studies of marine microorganisms — supported by new methods, tools and resources — are needed to help inform policies to mitigate the impact of climate change.
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Brief Communication |
Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats
A head-mounted three-photon microscope based on a custom-designed optical fiber and dispersion compensation enables imaging of activity from neuronal populations deep in the cortex of freely moving rats.
- Alexandr Klioutchnikov
- , Damian J. Wallace
- & Jason N. D. Kerr
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Brief Communication |
Deep three-photon imaging of the brain in intact adult zebrafish
Three-photon microscopy provides access to most of the adult zebrafish brain for both structural and functional imaging, as well as to the Danionella dracula brain.
- Dawnis M. Chow
- , David Sinefeld
- & Joseph R. Fetcho
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Article |
Multiplexed Cre-dependent selection yields systemic AAVs for targeting distinct brain cell types
M-CREATE is an in vivo screening strategy for identifying recombinant AAVs with desired tropism. The approach involves both positive and negative selection and yields vectors with diversified cell-type tropism that can cross the blood–brain barrier in adult mice across strains when delivered intravenously.
- Sripriya Ravindra Kumar
- , Timothy F. Miles
- & Viviana Gradinaru
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Research Highlight |
Venomous organoids
Snake venom gland organoids provide a glimpse into the cell biology of the respective organ and serve as a platform for producing snake toxins.
- Nina Vogt
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This Month |
Ali Ertürk
Analyzing cleared tissue with a deep-learning pipeline, and why dreaming is good for science.
- Vivien Marx
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Article |
Machine learning analysis of whole mouse brain vasculature
VesSAP is a tissue clearing- and deep learning-based pipeline for comprehensively analyzing mouse vasculature, from large vessels to small capillaries.
- Mihail Ivilinov Todorov
- , Johannes Christian Paetzold
- & Ali Ertürk
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Research Highlight |
Photoacoustics in a snap
Photoacoustic topography through an ergodic relay increases imaging throughput and opens a path toward wearable devices.
- Nina Vogt
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This Month |
Na Ji
How joy and wide-ranging curiosity leads to neurobiology tools for new types of questions.
- Vivien Marx
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Brief Communication |
Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
High-speed two-photon laser scanning microscopy using a passive laser scanner based on free-space angular-chirp-enhanced delay achieves frame rates suitable for voltage imaging in vivo in the mouse brain.
- Jianglai Wu
- , Yajie Liang
- & Na Ji
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Brief Communication |
Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
Integrating a Bessel focus module into a two-photon fluorescence mesoscope enables high-speed volumetric imaging of neuronal activity in soma, dendrites and spines.
- Rongwen Lu
- , Yajie Liang
- & Na Ji
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Article |
A virtual reality system to analyze neural activity and behavior in adult zebrafish
Complex behaviors and the underlying neural activity in adult zebrafish can be accessed through a virtual reality system in combination with two-photon microscopy.
- Kuo-Hua Huang
- , Peter Rupprecht
- & Rainer W. Friedrich
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Research Highlight |
RAMPing up voltage indicator imaging
Combining a voltage sensor with random-access microscopy allows imaging of neuronal activity in behaving mice.
- Nina Vogt
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Brief Communication |
An adaptive excitation source for high-speed multiphoton microscopy
An adaptive excitation source enables two- and three-photon imaging of the awake mouse brain with high spatial and temporal resolution at 30-fold-reduced laser power relative to conventional approaches.
- Bo Li
- , Chunyan Wu
- & Chris Xu
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Brief Communication |
A CMOS NMR needle for probing brain physiology with high spatial and temporal resolution
A miniaturized NMR-on-a-chip needle can be implanted into rodent brains and can measure blood flow and oxygenation changes in vivo in a small volume at an unprecedentedly high temporal resolution of a few milliseconds.
- Jonas Handwerker
- , Marlon Pérez-Rodas
- & Klaus Scheffler
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Brief Communication |
Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution
An alternative to focused ion beam scanning electron microscopy (FIB-SEM), gas cluster ion beam scanning electron microscopy (GCIB-SEM) is compatible with large tissue samples while achieving similar isotropic resolution.
- Kenneth J. Hayworth
- , David Peale
- & Harald F. Hess
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Article |
Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits
Simultaneous two-photon microscopic and one-photon mesoscopic imaging of calcium signals enables correlation of local cellular and brain-wide network activity.
- Daniel Barson
- , Ali S. Hamodi
- & Michael J. Higley
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Research Highlight |
A bright future for voltage imaging
The hybrid voltage-indicator Voltron combines the voltage sensitivity of microbial rhodopsins with the brightness and photostability of chemical dyes.
- Nina Vogt
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Brief Communication |
Kilohertz two-photon brain imaging in awake mice
A multi-beam two-photon microscope enables imaging of calcium activity or neurovascular dynamics in the brain with millisecond-scale temporal resolution.
- Tong Zhang
- , Oscar Hernandez
- & Mark J. Schnitzer
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Article |
Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics
An engineering approach guided by machine learning results in high-performance channelrhodopsin variants that are suitable for systemic viral delivery and illumination through a thinned skull.
- Claire N. Bedbrook
- , Kevin K. Yang
- & Frances H. Arnold
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Article |
Depth-resolved fiber photometry with a single tapered optical fiber implant
Fiber photometry with tapered fibers allows monitoring of neural activity in larger volumes than with flat-cleaved fibers. In addition, signals from different depths can be resolved with the same tapered fiber.
- Filippo Pisano
- , Marco Pisanello
- & Ferruccio Pisanello
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Research Highlight |
Non-invasive and fast control of neural activity
Chemomagnetics enable remote and non-invasive modulation of neural activity in behaving mice.
- Nina Vogt
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Brief Communication |
4D functional ultrasound imaging of whole-brain activity in rodents
Functional ultrasound (fUS) imaging of neural activity has been extended to volumetric imaging across the whole brain. 4D fUS is demonstrated in the rat brain in response to sensory stimuli and during seizure-like activity.
- Claire Rabut
- , Mafalda Correia
- & Mickael Tanter
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Article |
Comprehensive mapping of neurotransmitter networks by MALDI–MS imaging
Mass spectrometry imaging with fluoromethylpyridinium-based reactive matrices allows mapping of neurotransmitters at high resolution and at a low limit of detection. The approach is applied to rat, macaque and human brain tissue samples for probing Parkinson’s disease-related changes.
- Mohammadreza Shariatgorji
- , Anna Nilsson
- & Per E. Andrén
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Research Highlight |
Looking at Hydra cells one at a time
Single-cell sequencing of Hydra results in a resource for studying differentiation trajectories, regeneration and nervous system development.
- Nina Vogt
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Comment |
Progress and challenges in analyzing rodent energy expenditure
Whole-body energy expenditure is the summed metabolic activities of tissues and, to remove the influence of body size, ratios of energy expenditure to body mass are often applied but can generate spurious differences. In 2011, a group of experts proposed adoption of ANCOVA for the analysis of metabolic rate but, seven years later, analyses based on ratios remain the most frequent. We discuss some of the barriers to adopting better analytical procedures.
- Rodrigo Fernández-Verdejo
- , Eric Ravussin
- & Jose E. Galgani
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Research Highlight |
Optogenetics turns up the heat
Illuminating the brain for optogenetic experiments can have undesirable effects on neural activity.
- Nina Vogt
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Article |
A cryo-FIB lift-out technique enables molecular-resolution cryo-ET within native Caenorhabditis elegans tissue
A technique to ‘lift out’ samples of interest from high-pressure-frozen specimens expands applications of cryo-electron tomography to multicellular organisms and tissue.
- Miroslava Schaffer
- , Stefan Pfeffer
- & Juergen M. Plitzko
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Article |
Kilohertz frame-rate two-photon tomography
A two-photon computed tomography approach, called scanned line angular projection microscopy, enables high-speed imaging at over 1 kHz frame rates, as demonstrated for glutamate imaging in the in vivo mouse brain.
- Abbas Kazemipour
- , Ondrej Novak
- & Kaspar Podgorski
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Article |
Gene expression atlas of a developing tissue by single cell expression correlation analysis
A method for analyzing scRNA-seq data sets based on correlations of gene expression allows construction of an atlas of the Drosophila wing disc.
- Josephine Bageritz
- , Philipp Willnow
- & Aurelio A. Teleman
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Article |
EPIC: software toolkit for elution profile-based inference of protein complexes
A software tool, EPIC, is developed to determine protein complex membership using chromatographic fractionation–mass spectrometry data, and is applied to map the global Caenorhabditis elegans interactome.
- Lucas ZhongMing Hu
- , Florian Goebels
- & Andrew Emili
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Research Highlight |
Voltage imaging in vivo
Developments in genetically encoded voltage indicators and imaging strategies enable the recording of multiple neurons with good signal-to-noise ratios in behaving mice.
- Nina Vogt