Research Highlight |
Featured
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Article |
Genome-wide SWAp-Tag yeast libraries for proteome exploration
A genome-wide collection of N-terminally tagged yeast libraries allows easy swapping of tags and exploration of the yeast proteome.
- Uri Weill
- , Ido Yofe
- & Maya Schuldiner
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Tools in Brief |
A yeast global genetic interaction map
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Brief Communication |
Micro-C XL: assaying chromosome conformation from the nucleosome to the entire genome
The combination of short and long crosslinkers during chromosome conformation capture allows the interrogation of structure from the nucleosome to the chromosome-wide level in yeast.
- Tsung-Han S Hsieh
- , Geoffrey Fudenberg
- & Oliver J Rando
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Research Highlights |
Micro-C maps of genome structure
A method using micrococcal nuclease for chromatin fragmentation gives a high-resolution view of 3D genome structure.
- Natalie de Souza
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Historical Commentary |
The yeast two-hybrid assay: still finding connections after 25 years
The idea of using hybrid proteins containing transcription factor domains to analyze protein-protein interactions was described in 1989. Over the past 25 years, this method has begun to reveal the complex protein networks that underlie cellular behavior.
- Marc Vidal
- & Stanley Fields
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Tools in Brief |
Chemical tools for yeast
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Methods in Brief |
A proteome in an hour
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Research Highlights |
Mass spectrometry mapmaking
A comprehensive mass spectrometric map of the yeast proteome is a valuable resource for both discovery-based and targeted proteome analyses.
- Allison Doerr
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Research Highlights |
The age of yeast
A simple microfluidic device allows long-term imaging of single budding yeast cells.
- Tal Nawy
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Article |
Systematic identification of gene annotation errors in the widely used yeast mutation collections
Comprehensive mutation libraries are an invaluable resource for screens in yeast, but anecdotal cases link phenotypes to the neighbor of a mutated gene rather than the mutant itself. The authors show that the neighboring gene effect is widespread and present software that uses protein-protein interaction networks to predict it.
- Taly Ben-Shitrit
- , Nir Yosef
- & Martin Kupiec
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Article |
Knocking out multigene redundancies via cycles of sexual assortment and fluorescence selection
By individually replacing 16 yeast genes encoding ABC transporters by GFP, mating and selecting for strains with accumulated mutations the authors create a Green Monster, a strain with deletions in all 16 genes.
- Yo Suzuki
- , Robert P St Onge
- & Frederick P Roth
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Article |
Quantitative analysis of fitness and genetic interactions in yeast on a genome scale
A statistical approach to quantitatively derive single and double mutant fitness from colony-based growth assays is described. The resultant SGA score permits assessment of yeast fitness and genetic interactions on the genome scale.
- Anastasia Baryshnikova
- , Michael Costanzo
- & Chad L Myers
