Editorial |
Featured
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Research Highlight |
In vivo traction force microscopy
Embryogram allows stress measurements during migration of the lateral line primordium in zebrafish embryos.
- Nina Vogt
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Article |
HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies
HYBRiD is a tissue-clearing approach that combines elements of solvent-based and aqueous, hydrogel-based methods, thereby achieving high transparency while maintaining fluorescence.
- Victoria Nudell
- , Yu Wang
- & Li Ye
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Article |
Detecting and correcting false transients in calcium imaging
SEUDO is a tool for detecting and correcting errors introduced by automated processing of calcium imaging data.
- Jeffrey L. Gauthier
- , Sue Ann Koay
- & Adam S. Charles
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Research Highlight |
Potent optogenetics
Two groups report improved variants of the channelrhodopsins ChRmine and ChroME, respectively.
- Nina Vogt
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Research Highlight |
Jellyfish enter neuroscience research
Jellyfish are established as a model system for neuroscience.
- Nina Vogt
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Research Highlight |
Flexibility in mesoscopic imaging
Two microscopy approaches bring flexibility to mesoscopic imaging in the brain, allowing independent imaging in multiple regions simultaneously.
- Nina Vogt
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Article |
Fast optical recording of neuronal activity by three-dimensional custom-access serial holography
3D-CASH is a random-access microscopy approach that avoids in vivo motion artifacts by sampling each targeted neuron with a holographically shaped grid of illumination spots. The technology allows recording neuronal activity in the mouse cortex at a throughput of 20,000 neurons per second.
- Walther Akemann
- , Sébastien Wolf
- & Laurent Bourdieu
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Article |
FlyWire: online community for whole-brain connectomics
FlyWire is an online community and a platform for proofreading electron microscopy-based connectome data of the Drosophila brain.
- Sven Dorkenwald
- , Claire E. McKellar
- & H. Sebastian Seung
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Article |
Cross-modal coherent registration of whole mouse brains
mBrainAligner is a cross-modal registration platform for whole mouse brains imaged with different modalities. In addition, a fluorescence micro-optical sectioning tomography-based mouse brain atlas has been generated.
- Lei Qu
- , Yuanyuan Li
- & Hanchuan Peng
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News & Views |
A repurposed vaccine grants durable anterograde access to neural circuits
How a decades-old vaccine for yellow fever may help scientists study the brain connectome.
- Jennifer B. Treweek
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Article |
Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D
Anterograde transneuronal tracing can be achieved with engineered variants of the yellow fever vaccine YFV-17D. Both monosynaptic and polysynaptic tracing are demonstrated in different circuits of the mouse brain.
- Elizabeth Li
- , Jun Guo
- & Wei Xu
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Research Highlight |
DELIVERing transgenes into muscle
Directed evolution of adeno-associated virus capsids with the DELIVER approach generates tissue-specific viruses.
- Nina Vogt
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Research Highlight |
A multimodal whole-body atlas
Researchers have registered a gene expression atlas to a whole-body EM volume of a marine bristle worm.
- Rita Strack
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Article |
An adaptive optics module for deep tissue multiphoton imaging in vivo
A compact adaptive optics module corrects aberrations in two-photon and three-photon microscopy, enabling structural and functional imaging deep in the mouse brain, the mouse spinal cord and the zebrafish larva.
- Cristina Rodríguez
- , Anderson Chen
- & Na Ji
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Article |
High-speed, cortex-wide volumetric recording of neuroactivity at cellular resolution using light beads microscopy
Light beads microscopy is a two-photon microscopy approach that allows high-speed volumetric imaging of neuronal activity at the mesoscale.
- Jeffrey Demas
- , Jason Manley
- & Alipasha Vaziri
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Article |
Chronically implantable LED arrays for behavioral optogenetics in primates
An implantable array of LEDs called the Opto-Array facilitates optogenetic manipulations in the nonhuman primate brain.
- Rishi Rajalingham
- , Michael Sorenson
- & Arash Afraz
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Article |
Clear optically matched panoramic access channel technique (COMPACT) for large-volume deep brain imaging
COMPACT allows imaging of large volumes in the brain through a periscope-like probe inside a glass capillary.
- Bowen Wei
- , Chenmao Wang
- & Meng Cui
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Research Highlight |
Optogenetic silencing at synaptic terminals
GPCR-based opsins can be harnessed to inhibit neurotransmission via inhibitory G protein signaling.
- Nina Vogt
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Research Highlight |
Cell–cell interactions revealed with RABID-seq
An approach involving barcoded viral tracing provides insights into cell–cell interactions at single-cell resolution.
- Nina Vogt
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Article |
Deep learning-enhanced light-field imaging with continuous validation
A deep learning–based algorithm enables efficient reconstruction of light-field microscopy data at video rate. In addition, concurrently acquired light-sheet microscopy data provide ground truth data for training, validation and refinement of the algorithm.
- Nils Wagner
- , Fynn Beuttenmueller
- & Anna Kreshuk
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Article |
Geometric deep learning enables 3D kinematic profiling across species and environments
DANNCE enables robust 3D tracking of animals’ limbs and other features in naturalistic environments by making use of a deep learning approach that incorporates geometric reasoning. DANNCE is demonstrated on behavioral sequences from rodents, marmosets, and chickadees.
- Timothy W. Dunn
- , Jesse D. Marshall
- & Bence P. Ölveczky
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Article |
Miniaturized head-mounted microscope for whole-cortex mesoscale imaging in freely behaving mice
The mini-mScope is a miniature microscope that can image neural activity at the mesoscale in most of the dorsal cortex of freely behaving mice.
- Mathew L. Rynes
- , Daniel A. Surinach
- & Suhasa B. Kodandaramaiah
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Brief Communication |
A three-dimensional virtual mouse generates synthetic training data for behavioral analysis
Bolaños et al. present a realistic three-dimensional virtual mouse model that can be animated and that facilitates the training of pose estimation algorithms.
- Luis A. Bolaños
- , Dongsheng Xiao
- & Timothy H. Murphy
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This Month |
Tiago Ferreira
How computational neuroanatomy, vintage gear and fado fit together.
- Vivien Marx
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Brief Communication |
SNT: a unifying toolbox for quantification of neuronal anatomy
SNT is a toolbox neuronal morphometry and connectomics and provides various analysis, visualization, quantification and modeling tools.
- Cameron Arshadi
- , Ulrik Günther
- & Tiago A. Ferreira
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This Month |
Qingming Luo
Connecting the Ming dynasty to sharper views in brain imaging.
- Vivien Marx
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Article |
High-definition imaging using line-illumination modulation microscopy
HD-fMOST is a microscopy technique for imaging large samples at high throughput and with high definition, which is achieved with a line-illumination modulation approach. The technology is illustrated by imaging fluorescently labeled neurons in whole mouse brains.
- Qiuyuan Zhong
- , Anan Li
- & Qingming Luo
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Research Highlight |
Real-time behavioral analysis
DeepLabCut-Live! performs pose estimation in real time and allows closed-loop feedback on behavior.
- Nina Vogt
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Brief Communication |
Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging
A two-photon miniature microscope with enlarged field of view and axial scanning capabilities has been developed and applied in freely moving mice.
- Weijian Zong
- , Runlong Wu
- & Heping Cheng
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Article |
Simultaneous cortex-wide fluorescence Ca2+ imaging and whole-brain fMRI
Simultaneous widefield calcium imaging and fMRI provide insight into neural activity at multiple scales and can be used to decipher the cellular origin of BOLD activity.
- Evelyn M. R. Lake
- , Xinxin Ge
- & R. Todd Constable
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Research Highlight |
X-ray connectomics
X-ray holographic nano-tomography is a promising complement to electron microscopy for connectomics studies.
- Nina Vogt
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This Month |
Lin Tian
Neurochemistry sensors for brain research and life with a collaborative frame of mind.
- Vivien Marx
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Research Highlight |
Volumetric imaging with confocal light field microscopy
Conferring optical sectioning capabilities on light field microscopy improves signal-to-noise ratio while maintaining high imaging speed.
- Nina Vogt
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Research Highlight |
Modeling the E. coli cell
An integrative modeling framework enables cross-validation of existing E. coli datasets and prediction of uncharacterized parameters.
- Lei Tang
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Article |
EthoLoop: automated closed-loop neuroethology in naturalistic environments
EthoLoop enables real-time tracking and behavioral analysis of animals in naturalistic environments and can be combined with behavioral conditioning, optogenetic stimulation or wireless recording of neural activity. The system is illustrated with freely behaving mice and mouse lemurs.
- Ali Nourizonoz
- , Robert Zimmermann
- & Daniel Huber
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Article |
An optimized acetylcholine sensor for monitoring in vivo cholinergic activity
A genetically encoded acetylcholine sensor with improved sensitivity allows detection of cholinergic neurotransmission in vivo in the Drosophila and mouse brain.
- Miao Jing
- , Yuexuan Li
- & Yulong Li
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Article |
In vivo detection of antigen-specific CD8+ T cells by immuno-positron emission tomography
Antigen-specific CD8+ T cells can be imaged by immunoPET with the help of synTacs, MHC-based tools that bind to relevant T-cell receptors.
- Andrew W. Woodham
- , Stad H. Zeigler
- & Hidde L. Ploegh
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Article |
An expanded palette of dopamine sensors for multiplex imaging in vivo
Red and yellow versions of the genetically encoded dopamine sensor dLight1 have been developed and allow multiplexed imaging of dopamine with neurotransmitter or cell-type-specific calcium combined with green sensors or actuators, as demonstrated ex vivo and in behaving rodents.
- Tommaso Patriarchi
- , Ali Mohebi
- & Lin Tian
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Research Highlight |
Connectomics in high throughput
BRICseq provides access to brain-wide connectivity and can be combined with functional and transcriptomic studies for a comprehensive view of neuronal circuitry.
- Nina Vogt
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Brief Communication |
High-sensitivity and high-specificity biomechanical imaging by stimulated Brillouin scattering microscopy
Stimulated Brillouin scattering microscopy overcomes the trade-off between acquisition speed and spectral resolution in spontaneous Brillouin scattering microscopy and allows visualization of elasticity and viscosity, as shown in C. elegans.
- Itay Remer
- , Roni Shaashoua
- & Alberto Bilenca
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Research Highlight |
Trapping fluorescence in the soma
Tethering fluorescent proteins to ribosomes clears up background signals when imaging neural circuits.
- Nina Vogt
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Article |
Optogenetic control of gene expression in plants in the presence of ambient white light
PULSE is an optogenetic tool that consists of two modules with different wavelength sensitivities. Their interplay enables optogenetic access to gene expression in plants independently of ambient light.
- Rocio Ochoa-Fernandez
- , Nikolaj B. Abel
- & Matias D. Zurbriggen
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Research Highlight |
Watching embryos develop
Embryonic development is typically hidden from view, but a window preparation technique now sheds light on this phase in the life of a mouse.
- Nina Vogt
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