News & Views |
Featured
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Letter |
Graphene-based metal-induced energy transfer for sub-nanometre optical localization
Using graphene as the ‘metal’ layer can increase the localization accuracy of metal-induced energy transfer, enabling axial localization of single emitters and measurement of the thickness of lipid bilayers with ångström accuracy.
- Arindam Ghosh
- , Akshita Sharma
- & Jörg Enderlein
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Article |
Optical coherence refraction tomography
By synthesizing undistorted cross-sectional image reconstructions from multiple conventional images acquired with angular diversity, optical coherence refraction tomography offers greater than threefold improvement in lateral resolution and speckle reduction in imaging tissue ultrastructure, and reconstructs the tissue’s internal refractive index distribution.
- Kevin C. Zhou
- , Ruobing Qian
- & Joseph A. Izatt
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News & Views |
Overcoming the colour barrier
High-efficiency, time-domain, near-infrared fluorophores provide multiplexed colour channels for distinct deep bioimaging.
- Shoujun Zhu
- & Xiaoyuan Chen
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Article |
Wavelength-encoded laser particles for massively multiplexed cell tagging
Intracellular laser particles based on silica-coated semiconductor microcavities with distinct emission wavelengths allow real-time tracking of thousands of cells in a tumour model.
- Nicola Martino
- , Sheldon J. J. Kwok
- & Seok-Hyun Yun
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Article |
Video-rate imaging of biological dynamics at centimetre scale and micrometre resolution
Video-rate imaging of the brains of awake mice with a field of view of 10 × 12 mm2 and a spatial resolution of 1.2 µm is accomplished.
- Jingtao Fan
- , Jinli Suo
- & Qionghai Dai
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News & Views |
Photonic amyloids
Emerging data reveal that amyloid fibrils possess intrinsic photonic activity, showing luminescence over a wide range of the electromagnetic spectrum from the ultraviolet to the near-infrared.
- Per Hammarström
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Article |
High-resolution, high-contrast mid-infrared imaging of fresh biological samples with ultraviolet-localized photoacoustic microscopy
Photoacoustic detection highly localized with a pulsed ultraviolet laser based on the Grüneisen relaxation effect allows water-background suppressed mid-infrared (MIR) imaging of lipids and proteins at ultraviolet resolution, at least an order of magnitude finer than the MIR diffraction limits.
- Junhui Shi
- , Terence T. W. Wong
- & Lihong V. Wang
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Article |
Ultraviolet–visible–near-infrared optical properties of amyloid fibrils shed light on amyloidogenesis
Two optical signatures of amyloid fibres—luminescence in the blue and a near-infrared signal, which can be observed in in vitro and in vivo tissues—are reported. The findings allow for staining-free characterization of amyloid deposits in human samples and could open the door to innovative diagnostic strategies for neurodegenerative diseases.
- Jonathan Pansieri
- , Véronique Josserand
- & Vincent Forge
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Article |
Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane
Interferometric scattering microscopy is employed to track proteins in live cell membranes, demonstrating tracking of transmembrane epidermal growth factor receptors with nanometre precision in all three dimensions at up to microsecond speeds and for durations of tens of minutes.
- Richard W. Taylor
- , Reza Gholami Mahmoodabadi
- & Vahid Sandoghdar
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Letter |
Ultrasensitive hyperspectral imaging and biodetection enabled by dielectric metasurfaces
Spatially resolved spectra from millions of pixels and information extraction from three molecules per μm2 is now possible using dielectric metasurfaces.
- Filiz Yesilkoy
- , Eduardo R. Arvelo
- & Hatice Altug
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News & Views |
Optical hooks
A type of near-field curved light field generated right at the output of a dielectric cuboid is experimentally observed. It is expected to have interesting applications in imaging and manipulation.
- Kishan Dholakia
- & Graham D. Bruce
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Letter |
Super-resolution retinal imaging using optically reassigned scanning laser ophthalmoscopy
The implementation of optically reassigned scanning laser ophthalmoscopy enables in vivo photon reassignment super-resolution imaging and high-resolution imaging of living human retinal cone photoreceptor cells without adaptive optics or chemical dilation of the eye.
- Theodore B. DuBose
- , Francesco LaRocca
- & Joseph A. Izatt
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News & Views |
Super-resolution with quantum light
Quantum correlations from photon antibunching enhance the resolution of image scanning microscopy in biological imaging by twofold, four times beyond the diffraction limit.
- Andrew Forbes
- & Valeria Rodriguez-Fajardo
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Editorial |
A long overdue recognition
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News & Views |
Intelligent learning with light
Artificial intelligence looks poised to drive the development of software and hardware platforms in the coming decades. In photonics, it is already proving invaluable and is having an impact in the areas of imaging, sensing and communications.
- Rachel Won
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Review Article |
Quantitative phase imaging in biomedicine
Over the past 10–15 years, quantitative phase imaging has moved from a research-driven to an application-focused field. This Review presents the main principles of operation and representative basic and clinical science applications.
- YongKeun Park
- , Christian Depeursinge
- & Gabriel Popescu
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Perspective |
Tackling standardization in fluorescence molecular imaging
The parameters and issues that affect the accuracy of fluorescence molecular imaging are discussed and a means for ensuring reliable reproduction of the fluorescence signals in biological tissue is proposed.
- Maximillian Koch
- , Panagiotis Symvoulidis
- & Vasilis Ntziachristos
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Article |
Nano-optic endoscope for high-resolution optical coherence tomography in vivo
A metalens is integrated into the design of an endoscopic optical coherence tomography catheter to achieve near-diffraction-limited imaging free of non-chromatic aberrations, offering high-resolution imaging well beyond the Rayleigh range of the input field.
- Hamid Pahlevaninezhad
- , Mohammadreza Khorasaninejad
- & Melissa J. Suter
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Article |
Femtosecond laser crosslinking of the cornea for non-invasive vision correction
Laser-driven crosslinking of molecules at the cornea offers a non-invasive alternative to surgical correction of the refractive power of the eye.
- Chao Wang
- , Mikhail Fomovsky
- & Sinisa Vukelic
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Article |
Enrichment of molecular antenna triplets amplifies upconverting nanoparticle emission
Lanthanide-doped upconverting nanoparticles exhibiting a 33,000 times increase in brightness and a 100 times increase in efficiency over bare upconverting nanoparticles are demonstrated. The findings are relevant in fields from solar energy to biophotonics.
- David J. Garfield
- , Nicholas J. Borys
- & P. James Schuck
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Article |
Combined multi-plane phase retrieval and super-resolution optical fluctuation imaging for 4D cell microscopy
By combining the sensitivity and high temporal resolution of phase imaging with the specificity and high spatial resolution of fluorescence microscopy, a 4D microscope is demonstrated that visualizes in three dimensions the fast cellular processes in living cells at up to 200 Hz.
- A. Descloux
- , K. S. Grußmayer
- & T. Lasser
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Article |
High-speed optical coherence tomography by circular interferometric ranging
Using an ultrafast, time-stretched frequency comb laser operating with repetition rates from 7.6 MHz to 18.9 MHz, a rapid and large-volumetric-field optical coherence tomography at an imaging rate of up to 7.5 volumes per second is demonstrated.
- Meena Siddiqui
- , Ahhyun S. Nam
- & Benjamin J. Vakoc
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Article |
Wavefront shaping with disorder-engineered metasurfaces
Using designer-disordered metasurfaces, optical input–output characteristics, which are typically difficult to obtain, can be known a priori. The approach is used for wavefront shaping, high-numerical-aperture focusing and fluorescence imaging.
- Mooseok Jang
- , Yu Horie
- & Changhuei Yang
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Review Article |
X-ray ptychography
This Review covers key advancements in X-ray ptychographic microscopy and tomography over the past ten years. Potential applications in the life and materials sciences, the latest concepts and future developments are also discussed.
- Franz Pfeiffer
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Letter |
Evanescent single-molecule biosensing with quantum-limited precision
An evanescent single-molecule biosensor that operates at the fundamental precision limit, allowing a four-order-of-magnitude reduction in optical intensity while maintaining state-of-the-art sensitivity, is demonstrated.
- N. P. Mauranyapin
- , L. S. Madsen
- & W. P. Bowen
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Letter |
Compressive hyperspectral time-resolved wide-field fluorescence lifetime imaging
Single-pixel system enables hyperspectral fluorescent lifetime imaging.
- Qi Pian
- , Ruoyang Yao
- & Xavier Intes
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Article |
Chip-based wide field-of-view nanoscopy
Nanoscopy on a chip makes it possible to perform super-resolution imaging of biological specimens with a wide field-of-view.
- Robin Diekmann
- , Øystein I. Helle
- & Balpreet S. Ahluwalia
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Progress Article |
The emergence of optical elastography in biomedicine
The principles and applications of optical elastography for use in biological imaging are reviewed.
- Brendan F. Kennedy
- , Philip Wijesinghe
- & David D. Sampson
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Article |
Background suppression in fluorescence nanoscopy with stimulated emission double depletion
Stimulated emission double depletion addresses the issue of background in super-resolution imaging and quantitative microscopy through implementation of a two-pulse sequence in a modified stimulated emission depletion set-up. The measured background intensity is removed from each voxel in the acquired images thanks to time-resolved detection.
- Peng Gao
- , Benedikt Prunsche
- & G. Ulrich Nienhaus
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Article |
Scattering compensation by focus scanning holographic aberration probing (F-SHARP)
A non-invasive scattering compensation method, termed F-SHARP, gives direct access to the phase and amplitude of the electric-field point spread function, enabling fast and high-resolution correction of aberrations and scattering in living tissue.
- Ioannis N. Papadopoulos
- , Jean-Sébastien Jouhanneau
- & Benjamin Judkewitz
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Letter |
Promotion of protein crystal growth by actively switching crystal growth mode via femtosecond laser ablation
The protein crystal growth mechanism can be changed from planar 2D nucleation growth to spiral growth by femtosecond laser ablation. By using this method, the growth rate of a hen egg-white lysozyme crystal increases from 0.3 µm per day to 3.4 µm per day.
- Yusuke Tominaga
- , Mihoko Maruyama
- & Yusuke Mori
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Letter |
Super-resolution imaging of multiple cells by optimized flat-field epi-illumination
An epi-illumination system based on microlens arrays enables field-independent imaging of multiple cells with nanoscale resolution and large field of views.
- Kyle M. Douglass
- , Christian Sieben
- & Suliana Manley
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Letter |
Multicolour localization microscopy by point-spread-function engineering
Multicolour tracking and super-resolution imaging is made possible by the use of a new design of optical phase mask.
- Yoav Shechtman
- , Lucien E. Weiss
- & W. E. Moerner
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Letter |
In vivo cellular-resolution retinal imaging in infants and children using an ultracompact handheld probe
A handheld probe enables retinal imaging in children.
- Francesco LaRocca
- , Derek Nankivil
- & Joseph A. Izatt
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News & Views |
Next step for super-resolution
The challenges of applying super-resolution imaging to live cells and providing meaningful information about real problems in biology were discussed in detail at ICON Europe in Basel.
- Oliver Graydon
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Article |
Stain-free histopathology by programmable supercontinuum pulses
A single-source multimodal nonlinear optical imaging system has been developed to probe different endogenous biomolecules. Rapid, stain-free imaging of fresh tissue specimens is possible with short turnaround times for disease diagnosis.
- Haohua Tu
- , Yuan Liu
- & Stephen A. Boppart
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Letter |
Removing orientation-induced localization biases in single-molecule microscopy using a broadband metasurface mask
A custom-designed metamaterial mask helps improve the accuracy of microscopy of single fluorescent molecules.
- Mikael P. Backlund
- , Amir Arbabi
- & W. E. Moerner
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Article |
Polarization control in an X-ray free-electron laser
Tunable polarization control and a two-colour X-ray pump–X-ray probe operating mode are demonstrated at the Linac Coherent Light Source (LCLS).
- Alberto A. Lutman
- , James P. MacArthur
- & Heinz-Dieter Nuhn
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Article |
Coordinate-targeted fluorescence nanoscopy with multiple off states
By exploiting a second off state of a reversibly switchable fluorophore, a general approach that can reduce photobleaching and enhance resolution of coordinate-targeted fluorescence nanoscopy has been demonstrated.
- Johann G. Danzl
- , Sven C. Sidenstein
- & Stefan W. Hell
Video-rate gigapixel imaging of the brain
Fast polarization sensitivity
Incoherent success
Graded-index squid eyes
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