Volume 19 Issue 1, January 2024

This protocol enables genetic manipulation of nuclear and cytoplasmic giant viruses and their host, Acanthomoeba castellanii, by using either CRISPR–Cas9 or homologous recombination.

An X-ray microscopy approach for 2D or 3D label-free visualization of the intracellular distribution of nanomedicines and the morphology of organelles in primary blood cells, macrophages, dendritic cells, monocytes and cancer cells.

This protocol presents a computational approach to scoring drug sensitivity that integrates multiple dose–response parameters into a single response metric and identifies differences in drug-response patterns between cancer cells and healthy control cells.

This protocol provides guidelines for performing single-cell combinatorial indexing cleavage under targets and tagmentation. This method builds on the existing cleavage under targets and tagmentation method and uses a combinatorial indexing step to allow single-cell profiling of chromatin modifications.

This protocol describes an assay to control mechanical forces during the natural flexion-extension cycle of the femorotibial joint of adult living fruit flies and to monitor fluorescent mechanosensitive reporters expressed in the neurons responsible for mechanosensation

Structural and functional analysis of human proteins requires their isolation in high yields and purity. Here, protease-cleavable, high-affinity, tag-specific nanobodies are used for the isolation of soluble and membrane proteins from lentiviral-transduced human cell suspensions.

CONIPHER is a computational framework for accurately inferring subclonal structure and the phylogenetic tree from multisample tumor sequencing, accounting for both copy number alterations and mutation errors.

This protocol describes a reproducible and reliable method for excisional skin wound healing assays in mice. The use of lineage-tracing assays to investigate the contribution of different cell populations to the repair process is also discussed.

This protocol describes barcoding bacteria for identification and quantification, a method for identifying and quantifying bacterial cells in microbiota samples based on the droplet-based barcoding and amplification of 16S rRNA genes from single bacterial cells.

Angstrom-scale two-dimensional channel devices can be assembled with precise control over their dimensions, as a single channel or hundreds of channels using layered crystals, and enable the measurement of angstrom-scale gas, ion and water fluidics.