Volume 19 Issue 2, February 2024

Tail-to-head terpene cyclization is an essential reaction for terpene synthesis, but it is difficult to achieve without an enzyme. This protocol describes the preparation and use of a hexameric resorcin[4]arene capsule as an artificial enzyme.

Obtaining large, uniform crystals of polymer materials, including covalent organic frameworks, is challenging. This protocol describes a fast, effective approach to making crystalline two- and three-dimensional covalent organic frameworks using supercritical CO₂ as the solvent.

This protocol for the spatiotemporal control of RNA activity uses LicV, a synthetic, photoswitchable RNA-binding protein (RBP) that can bind to a specific RNA sequence in response to blue light irradiation, and provides an efficient and generalizable strategy for engineering photoswitchable RBPs.

The recently available 28.2 Tesla nuclear magnetic resonance spectrometers (1.2 GHz ¹H frequency) can be used to characterize highly flexible proteins and protein regions. This protocol explains how to setup ¹³C nuclear magnetic resonance experiments to achieve optimal results.

The detailed design and fabrication of small-scale magnetic soft-bodied robots with multimodal locomotion capability, including the processes required for locomotion control and optimization.

Single-cell multiple displacement amplification with the variant caller SCcaller ensures high-fidelity, quantitative analysis of single-nucleotide variations and small insertions and deletions.

This protocol presents a method for quantitative mapping of pseudouridine modifications in the cellular transcriptome at single-base resolution.

Two-dimensional protein films, prepared via amyloid-like aggregation, can be made with nano- to meter-scale dimensions and features such as high interface adhesion, as well as tunable antibacterial, biomineralization and antifouling activity.

An automated workflow for culturing human induced pluripotent stem cells expressing fluorescently tagged proteins, and seeding them on 96-well optical-grade, glass-bottom plates for high-quality, live-cell three-dimensional microscopy on a large scale.