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Volume 4 No 12
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Nature protocols
Assessment of pyrogenic contaminations with validated human whole-blood assay - pp1709 - 1721
Mardas Daneshian, Sonja von Aulock & Thomas Hartung
Published online: 05 November 2009 | doi:10.1038/nprot.2009.159
Abstract - Assessment of pyrogenic contaminations with validated human whole-blood assay | Full Text - Assessment of pyrogenic contaminations with validated human whole-blood assay | PDF (620 KB) - Assessment of pyrogenic contaminations with validated human whole-blood assay
Subject Categories: Immunological techniques | Pharmacology and toxicology
Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays - pp1722 - 1736
Susan M Gribble, Bee Ling Ng, Elena Prigmore, Tomas Fitzgerald & Nigel P Carter
Published online: 05 November 2009 | doi:10.1038/nprot.2009.183
Abstract - Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays | Full Text - Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays | PDF (1,089 KB) - Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
Subject Categories: Genomics and proteomics | Isolation, purification and separation | Nucleic acid based molecular biology
Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia - pp1737 - 1748
Anne Limbourg, Thomas Korff, L Christian Napp, Wolfgang Schaper, Helmut Drexler & Florian P Limbourg
Published online: 05 November 2009 | doi:10.1038/nprot.2009.185
Abstract - Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia | Full Text - Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia | PDF (1,519 KB) - Evaluation of postnatal arteriogenesis and angiogenesis in a mouse model of hind-limb ischemia
Subject Categories: Model organisms | Imaging
Measuring bone blood supply in mice using fluorescent microspheres - pp1749 - 1758
Maria A Serrat
Published online: 05 November 2009 | doi:10.1038/nprot.2009.190
Abstract - Measuring bone blood supply in mice using fluorescent microspheres | Full Text - Measuring bone blood supply in mice using fluorescent microspheres | PDF (393 KB) - Measuring bone blood supply in mice using fluorescent microspheres
Subject Category: Model organisms
High-yield cell-free protein production from P-gel - pp1759 - 1770
Nokyoung Park, Jason S Kahn, Edward J Rice, Mark R Hartman, Hisakage Funabashi, Jianfeng Xu, Soong Ho Um & Dan Luo
Published online: 12 November 2009 | doi:10.1038/nprot.2009.174
Abstract - High-yield cell-free protein production from P-gel | Full Text - High-yield cell-free protein production from P-gel | PDF (885 KB) - High-yield cell-free protein production from P-gel
Subject Category: Biochemistry and protein analysis
High-throughput haplotype determination over long distances by haplotype fusion PCR and ligation haplotyping - pp1771 - 1783
Daniel J Turner & Matthew E Hurles
Published online: 12 November 2009 | doi:10.1038/nprot.2009.184
Abstract - High-throughput haplotype determination over long distances by haplotype fusion PCR and ligation haplotyping | Full Text - High-throughput haplotype determination over long distances by haplotype fusion PCR and ligation haplotyping | PDF (662 KB) - High-throughput haplotype determination over long distances by haplotype fusion PCR and ligation haplotyping
Subject Categories: Genetic analysis | Nucleic acid based molecular biology
Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code - pp1784 - 1789
Chang C Liu, Susan E Cellitti, Bernhard H Geierstanger & Peter G Schultz
Published online: 12 November 2009 | doi:10.1038/nprot.2009.188
Abstract - Efficient expression of tyrosine-sulfated proteins in : E. coli: using an expanded genetic code | Full Text - Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code | PDF (319 KB) - Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code
Subject Categories: Biochemistry and protein analysis | Isolation, purification and separation | Synthetic chemistry
Simultaneous analysis of reactive oxygen species and reduced glutathione content in living cells by polychromatic flow cytometry - pp1790 - 1797
Andrea Cossarizza, Roberta Ferraresi, Leonarda Troiano, Erika Roat, Lara Gibellini, Linda Bertoncelli, Milena Nasi & Marcello Pinti
Published online: 12 November 2009 | doi:10.1038/nprot.2009.189
Abstract - Simultaneous analysis of reactive oxygen species and reduced glutathione content in living cells by polychromatic flow cytometry | Full Text - Simultaneous analysis of reactive oxygen species and reduced glutathione content in living cells by polychromatic flow cytometry | PDF (736 KB) - Simultaneous analysis of reactive oxygen species and reduced glutathione content in living cells by polychromatic flow cytometry
Subject Categories: Cell and developmental biology | Immunological techniques | Isolation, purification and separation | Pharmacology and toxicology
Protocols to detect senescence-associated beta-galactosidase (SA-
gal) activity, a biomarker of senescent cells in culture and in vivo - pp1798 - 1806
Florence Debacq-Chainiaux, Jorge D Erusalimsky, Judith Campisi & Olivier Toussaint
Published online: 19 November 2009 | doi:10.1038/nprot.2009.191
Abstract - Protocols to detect senescence-associated beta-galactosidase (SA-[beta]gal) activity, a biomarker of senescent cells in culture and : in vivo | Full Text - Protocols to detect senescence-associated beta-galactosidase (SA-
gal) activity, a biomarker of senescent cells in culture and in vivo | PDF (461 KB) - Protocols to detect senescence-associated beta-galactosidase (SA-gal) activity, a biomarker of senescent cells in culture and in vivo
Subject Categories: Cell and developmental biology | Cell and tissue culture | Pharmacology and toxicology
Determination of gene expression patterns using in situ hybridization to Drosophila testes - pp1807 - 1819
Ceri A Morris, Elizabeth Benson & Helen White-Cooper
Published online: 19 November 2009 | doi:10.1038/nprot.2009.192
Abstract - Determination of gene expression patterns using : in situ: hybridization to : Drosophila: testes | Full Text - Determination of gene expression patterns using in situ hybridization to Drosophila testes | PDF (944 KB) - Determination of gene expression patterns using in situ hybridization to Drosophila testes
Subject Categories: Genetic analysis | Model organisms | Nucleic acid based molecular biology
Global identification of protein kinase substrates by protein microarray analysis - pp1820 - 1827
Janine Mok, Hogune Im & Michael Snyder
Published online: 19 November 2009 | doi:10.1038/nprot.2009.194
Abstract - Global identification of protein kinase substrates by protein microarray analysis | Full Text - Global identification of protein kinase substrates by protein microarray analysis | PDF (403 KB) - Global identification of protein kinase substrates by protein microarray analysis
Subject Categories: Biochemistry and protein analysis | Genomics and proteomics
Synchronous culture of Plasmodium falciparum at high parasitemia levels - pp1828 - 1844
Azar Radfar, Darío Méndez, Carlos Moneriz, María Linares, Patricia Marín-García, Antonio Puyet, Amalia Diez & José M Bautista
Published online: 19 November 2009 | doi:10.1038/nprot.2009.198
Abstract - Synchronous culture of : Plasmodium falciparum: at high parasitemia levels | Full Text - Synchronous culture of Plasmodium falciparum at high parasitemia levels | PDF (1,476 KB) - Synchronous culture of Plasmodium falciparum at high parasitemia levels
Subject Categories: Cell and tissue culture | Model organisms
