Cell-PLoc: a package of Web servers for predicting subcellular localization of proteins in various organisms - pp153 - 162

Kuo-Chen Chou & Hong-Bin Shen

Published online: 17 January 2008 | doi:10.1038/nprot.2007.494

Abstract - Cell-PLoc: a package of Web servers for predicting subcellular localization of proteins in various organisms | Full Text - Cell-PLoc: a package of Web servers for predicting subcellular localization of proteins in various organisms | PDF (716 KB) - Cell-PLoc: a package of Web servers for predicting subcellular localization of proteins in various organisms

Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances - pp163 - 175

Irith Wiegand, Kai Hilpert & Robert E W Hancock

Published online: 17 January 2008 | doi:10.1038/nprot.2007.521

Abstract - Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances | Full Text - Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances | PDF (422 KB) - Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances

A continuous process to extract plasmid DNA based on alkaline lysis - pp176 - 180

Xiaolin Li, Huali Jin, Zhifang Wu, Simon Rayner & Bin Wang

Published online: 17 January 2008 | doi:10.1038/nprot.2007.526

Abstract - A continuous process to extract plasmid DNA based on alkaline lysis | Full Text - A continuous process to extract plasmid DNA based on alkaline lysis | PDF (187 KB) - A continuous process to extract plasmid DNA based on alkaline lysis

Agrobacterium-mediated transformation of Brassica napus and Brassica oleracea - pp181 - 189

Prem L Bhalla & Mohan B Singh

Published online: 17 January 2008 | doi:10.1038/nprot.2007.527

Abstract - Agrobacterium: -mediated transformation of : Brassica napus: and : Brassica oleracea | Full Text - Agrobacterium-mediated transformation of Brassica napus and Brassica oleracea | PDF (443 KB) - Agrobacterium-mediated transformation of Brassica napus and Brassica oleracea

MicroRNA detection by northern blotting using locked nucleic acid probes - pp190 - 196

Éva Várallyay, József Burgyán & Zoltán Havelda

Published online: 17 January 2008 | doi:10.1038/nprot.2007.528

Abstract - MicroRNA detection by northern blotting using locked nucleic acid probes | Full Text - MicroRNA detection by northern blotting using locked nucleic acid probes | PDF (199 KB) - MicroRNA detection by northern blotting using locked nucleic acid probes

Protocol for the production of viable bimaternal mouse embryos - pp197 - 209

Manabu Kawahara, Yayoi Obata, Yusuke Sotomaru, Nobuhiro Shimozawa, Siqin Bao, Toshitaka Tsukadaira, Atsushi Fukuda & Tomohiro Kono

Published online: 17 January 2008 | doi:10.1038/nprot.2007.531

Abstract - Protocol for the production of viable bimaternal mouse embryos | Full Text - Protocol for the production of viable bimaternal mouse embryos | PDF (520 KB) - Protocol for the production of viable bimaternal mouse embryos

Simple protocols for NMR analysis of the enantiomeric purity of chiral primary amines - pp210 - 214

Yolanda Pérez-Fuertes, Andrew M Kelly, John S Fossey, Magdalena E Powell, Steven D Bull & Tony D James

Published online: 17 January 2008 | doi:10.1038/nprot.2007.524

Abstract - Simple protocols for NMR analysis of the enantiomeric purity of chiral primary amines | Full Text - Simple protocols for NMR analysis of the enantiomeric purity of chiral primary amines | PDF (208 KB) - Simple protocols for NMR analysis of the enantiomeric purity of chiral primary amines

See also: Protocol by Kelly et al.

Simple protocols for NMR analysis of the enantiomeric purity of chiral diols - pp215 - 219

Andrew M Kelly, Yolanda Pérez-Fuertes, John S Fossey, Sonia Lozano Yeste, Steven D Bull & Tony D James

Published online: 17 January 2008 | doi:10.1038/nprot.2007.523

Abstract - Simple protocols for NMR analysis of the enantiomeric purity of chiral diols | Full Text - Simple protocols for NMR analysis of the enantiomeric purity of chiral diols | PDF (218 KB) - Simple protocols for NMR analysis of the enantiomeric purity of chiral diols

See also: Protocol by Pérez-Fuertes et al.

Fluorescence and chromogenic in situ hybridization to detect genetic aberrations in formalin-fixed paraffin embedded material, including tissue microarrays - pp220 - 234

Brenda Summersgill, Jeremy Clark & Janet Shipley

Published online: 24 January 2008 | doi:10.1038/nprot.2007.534

Abstract - Fluorescence and chromogenic : in situ: hybridization to detect genetic aberrations in formalin-fixed paraffin embedded material, including tissue microarrays | Full Text - Fluorescence and chromogenic in situ hybridization to detect genetic aberrations in formalin-fixed paraffin embedded material, including tissue microarrays | PDF (374 KB) - Fluorescence and chromogenic in situ hybridization to detect genetic aberrations in formalin-fixed paraffin embedded material, including tissue microarrays

Direct detection of N-HO=C hydrogen bonds in biomolecules by NMR spectroscopy - pp235 - 241

Florence Cordier, Lydia Nisius, Andrew J Dingley & Stephan Grzesiek

Published online: 24 January 2008 | doi:10.1038/nprot.2007.498

Abstract - Direct detection of N-H[ctdot]O=C hydrogen bonds in biomolecules by NMR spectroscopy | Full Text - Direct detection of N-HO=C hydrogen bonds in biomolecules by NMR spectroscopy | PDF (322 KB) - Direct detection of N-HO=C hydrogen bonds in biomolecules by NMR spectroscopy | Supplementary information

Direct detection of N-HN hydrogen bonds in biomolecules by NMR spectroscopy - pp242 - 248

Andrew J Dingley, Lydia Nisius, Florence Cordier & Stephan Grzesiek

Published online: 24 January 2008 | doi:10.1038/nprot.2007.497

Abstract - Direct detection of N-H[ctdot]N hydrogen bonds in biomolecules by NMR spectroscopy | Full Text - Direct detection of N-HN hydrogen bonds in biomolecules by NMR spectroscopy | PDF (281 KB) - Direct detection of N-HN hydrogen bonds in biomolecules by NMR spectroscopy | Supplementary information

In vitro functional imaging in brain slices using fast voltage-sensitive dye imaging combined with whole-cell patch recording - pp249 - 255

Greg C Carlson & Douglas A Coulter

Published online: 24 January 2008 | doi:10.1038/nprot.2007.539

Abstract - In vitro: functional imaging in brain slices using fast voltage-sensitive dye imaging combined with whole-cell patch recording | Full Text - In vitro functional imaging in brain slices using fast voltage-sensitive dye imaging combined with whole-cell patch recording | PDF (328 KB) - In vitro functional imaging in brain slices using fast voltage-sensitive dye imaging combined with whole-cell patch recording

Membrane reconstitution of ABC transporters and assays of translocator function - pp256 - 266

Eric R Geertsma, N A B Nik Mahmood, Gea K Schuurman-Wolters & Bert Poolman

Published online: 31 January 2008 | doi:10.1038/nprot.2007.519

Abstract - Membrane reconstitution of ABC transporters and assays of translocator function | Full Text - Membrane reconstitution of ABC transporters and assays of translocator function | PDF (360 KB) - Membrane reconstitution of ABC transporters and assays of translocator function | Supplementary information

Parallel tagged sequencing on the 454 platform - pp267 - 278

Matthias Meyer, Udo Stenzel & Michael Hofreiter

Published online: 31 January 2008 | doi:10.1038/nprot.2007.520

Abstract - Parallel tagged sequencing on the 454 platform | Full Text - Parallel tagged sequencing on the 454 platform | PDF (363 KB) - Parallel tagged sequencing on the 454 platform | Supplementary information

Direct detection of small RNAs using splinted ligation - pp279 - 287

Patricia A Maroney, Sangpen Chamnongpol, Frédéric Souret & Timothy W Nilsen

Published online: 31 January 2008 | doi:10.1038/nprot.2007.530

Abstract - Direct detection of small RNAs using splinted ligation | Full Text - Direct detection of small RNAs using splinted ligation | PDF (257 KB) - Direct detection of small RNAs using splinted ligation

Monitoring structural changes in nucleic acids with single residue spatial and millisecond time resolution by quantitative hydroxyl radical footprinting - pp288 - 302

Inna Shcherbakova & Michael Brenowitz

Published online: 07 February 2008 | doi:10.1038/nprot.2007.533

Abstract - Monitoring structural changes in nucleic acids with single residue spatial and millisecond time resolution by quantitative hydroxyl radical footprinting | Full Text - Monitoring structural changes in nucleic acids with single residue spatial and millisecond time resolution by quantitative hydroxyl radical footprinting | PDF (1,858 KB) - Monitoring structural changes in nucleic acids with single residue spatial and millisecond time resolution by quantitative hydroxyl radical footprinting

High-resolution circular chromosome conformation capture assay - pp303 - 313

Anita Göndör, Carole Rougier & Rolf Ohlsson

Published online: 07 February 2008 | doi:10.1038/nprot.2007.540

Abstract - High-resolution circular chromosome conformation capture assay | Full Text - High-resolution circular chromosome conformation capture assay | PDF (280 KB) - High-resolution circular chromosome conformation capture assay

Directional conjugation of antibodies to nanoparticles for synthesis of multiplexed optical contrast agents with both delivery and targeting moieties - pp314 - 320

S Kumar, J Aaron & K Sokolov

Published online: 07 February 2008 | doi:10.1038/nprot.2008.1

Abstract - Directional conjugation of antibodies to nanoparticles for synthesis of multiplexed optical contrast agents with both delivery and targeting moieties | Full Text - Directional conjugation of antibodies to nanoparticles for synthesis of multiplexed optical contrast agents with both delivery and targeting moieties | PDF (466 KB) - Directional conjugation of antibodies to nanoparticles for synthesis of multiplexed optical contrast agents with both delivery and targeting moieties

miChip: an array-based method for microRNA expression profiling using locked nucleic acid capture probes - pp321 - 329

Mirco Castoldi, Sabine Schmidt, Vladimir Benes, Matthias W Hentze & Martina U Muckenthaler

Published online: 07 February 2008 | doi:10.1038/nprot.2008.4

Abstract - miChip: an array-based method for microRNA expression profiling using locked nucleic acid capture probes | Full Text - miChip: an array-based method for microRNA expression profiling using locked nucleic acid capture probes | PDF (282 KB) - miChip: an array-based method for microRNA expression profiling using locked nucleic acid capture probes

Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai transmission electron microscopes - pp330 - 339

Robert A Grassucci, Derek Taylor & Joachim Frank

Published online: 07 February 2008 | doi:10.1038/nprot.2007.474

Abstract - Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai transmission electron microscopes | Full Text - Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai transmission electron microscopes | PDF (680 KB) - Visualization of macromolecular complexes using cryo-electron microscopy with FEI Tecnai transmission electron microscopes