Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
mNET-seq generates genome-wide, single-nucleotide–resolution data on Pol II occupancy and co-transcriptional RNA processing, with the unique ability to link these processes to Pol II C-terminal domain phosphorylation states.
This protocol uses a microfluidic flow-focusing device to encapsulate single cells, enabling high-throughput sequencing of the paired immune receptor repertoire from millions of lymphocytes at the single-cell level.
Canfarotta et al. describe an elegant molecular imprinting method in which the target is immobilized on glass beads. These are then used to produce nanoparticles, with binding properties analogous to those of antibodies.
In this protocol, Hung et al. describe a method for performing cell compartment–specific proteomics for regions of interest using the engineered ascorbate peroxidase APEX2.
Here the authors describe cP-RNA-seq to selectively amplify and sequence cyclic phosphate (cP)-containing RNAs. This method extends the utility of standard RNA-seq to the assessment of cP-containing RNA repertoires in various transcriptomes.
This protocol describes how to reconstruct and culture the freshwater rainbow trout gill epithelium. This model can be used to study gill physiology and has applications in toxicity testing, bioaccumulation studies and water quality monitoring.
This protocol describes how to sequence the transcriptome from a single nucleus. It is particularly suited to cell types that are difficult to isolate as intact whole cells, such as neurons.
Venous access catheters used in clinics are prone to biofilm contamination, contributing to chronic infections. Here the authors provide a protocol to allow the in vivo study of catheter-associated biofilm infections in a TIVAP rat model.
Transcription factories contain all three mammalian RNA polymerases, each actively transcribing a different subset of genes. This protocol describes how to isolate large factory fragments for the analysis of associated protein and RNA content.
This protocol describes how to use miniature, integrated microscopes in conjunction with an implantable microendoscopic lens to guide light into and out of the brain, thereby enabling optical access to the brain.
Genome editing using designer nucleases such as TALENs or the CRISPR-Cas9 system is hampered by a lack of methods to detect and quantify the products. Here the authors present GEF-dPCR, a droplet-based digital PCR method for assessing gene-editing frequencies.