Neural probe protocols

This tutorial describes a set of essential performance tests for characterization of neural interface electrodes. The authors provide guidelines for standardized implementation and reporting on electrode performances.

Neural interfaces with implantable electrodes are used to modulate and restore function to the peripheral nervous system. Hybrid modeling described in this protocol is used to optimize each aspect of the implantable electrode design and operation.

This Protocol documents the extensive preparation required to adopt Neuropixels probes for use in patients for recording spiking activity from up to hundreds of cortical neurons and associating it with local field potentials.

Simultaneous electrophysiological recordings are obtained from multiple brain regions using Neuropixels probes. The procedure covers the implantation of a headframe, the targeted positioning of probes and their insertion through a perforated imaging window.

This Protocol Extension describes the fabrication and implantation of 3D-printed neural probes for tethered or wireless optogenetics in freely moving rodents.

An approach to reproducing episodes of hypotension-mimicking orthostatic challenges while monitoring arterial blood pressure and sympathetic nerve activity over several months in preclinical models of chronic neurological disorders, using a combination of available telemetry technologies, optogenetics and neuronal tract tracing.

This protocol describes the surgical procedures for implantation of a wireless, battery-free optogenetic implant (NeuroLux spinal optogenetic device) for optogenetic control of spinal cord circuits in mice.

This protocol describes the implantation of Neuropixels probes for chronic recording of neural activity in rats and mice using 3D-printed fixtures. The fixtures enable routine probe reuse, and single-, dual- and movable-probe variants are described.

This protocol describes how to use miniature, integrated microscopes in conjunction with an implantable microendoscopic lens to guide light into and out of the brain, thereby enabling optical access to the brain.

The protocol describes how to make transparent graphene neural electrodes for implantation onto the surface of the cerebral cortex in rodents and subsequent neural analysis by fluorescence microscopy, electrophysiology, optical coherence tomography, and optogenetics.

This protocol extension describes the fabrication of optofluidic neural probes and implantation for advanced in vivo pharmacology and optogenetics in freely moving rodents.