Science for the people - pp371 - 372

doi:10.1038/ncb0503-371

Full Text - Science for the people | PDF (56 KB) - Science for the people

The N-end rule and regulation of apoptosis - pp373 - 376

Alexander Varshavsky

doi:10.1038/ncb0503-373

The ubiquitin-dependent N-end rule pathway targets proteins for degradation through their destabilizing N-terminal residues. This pathway is known to control the import of peptides, chromosome stability and cardiovascular development. A new report identifies yet another function of the N-end rule pathway: the regulation of apoptosis through degradation of Drosophila melanogaster DIAP1.

Full Text - The N-end rule and regulation of apoptosis | PDF (1,067 KB) - The N-end rule and regulation of apoptosis

Morphogens recycled - p376

Alison Schuldt

doi:10.1038/ncb0503-376

Full Text - Morphogens recycled | PDF (512 KB) - Morphogens recycled

Split personalities: the agonistic antagonist Sprouty - pp377 - 379

Gerhard Christofori

doi:10.1038/ncb0503-377

Sprouty proteins are antagonists of receptor tyrosine kinase (RTK) signalling in a number of developmental and physiological processes. A collection of work indicates that Sprouty proteins negatively and positively modulate RTK-induced signalling pathways through various mechanisms, thereby combining a variety of cellular functions in one molecule.

Full Text - Split personalities: the agonistic antagonist Sprouty | PDF (115 KB) - Split personalities: the agonistic antagonist Sprouty

Monopolar attachment by Polo - pp379 - 382

Yoshinori Watanabe

doi:10.1038/ncb0503-379

A distinguishing feature of meiosis is a reductional division where homologous chromosomes — rather than sister chromatids — are pulled to opposite poles. Polo-like kinase (Plk), a prominent regulator of mitotic progression in Saccharomyces cerevisiae, is also crucial in regulating chromosome and chromatid separation during meiosis.

Full Text - Monopolar attachment by Polo | PDF (118 KB) - Monopolar attachment by Polo

Sliding doors: clathrin-coated pits or caveolae? - pp382 - 384

Michela Felberbaum-Corti, Françoise Gisou Van Der Goot & Jean Gruenberg

doi:10.1038/ncb0503-382

Some cell-surface receptors activate downstream signal transduction pathways not only from the cell surface but also from endosomes, suggesting that signalling pathways can be regulated by compartmentalization. A further twist is that different internalization routes seem to predetermine whether transforming growth factor (TGF-) receptors will trigger a signalling response or be degraded.

Full Text - Sliding doors: clathrin-coated pits or caveolae? | PDF (104 KB) - Sliding doors: clathrin-coated pits or caveolae?

Deafening cycle - pp385 - 387

Bridget Baumgartner & J. Wade Harper

doi:10.1038/ncb0503-385

Hearing loss in mammals occurs when auditory hair cells, specialized in the detection of sound waves, undergo irreversible damage. However, the mechanism, and in particular the genetic basis of the process, is poorly understood. A study in this issue now shows that functional inactivation of the cyclin D-dependent kinase inhibitor Ink4d results in progressive hearing loss in mice, implicating Cdk inhibitors and inappropriate hair cell proliferation in deafness.

Full Text - Deafening cycle | PDF (253 KB) - Deafening cycle

Small changes in Wnt signalling - p387

Sarah Greaves

doi:10.1038/ncb0503-387

Full Text - Small changes in Wnt signalling | PDF (156 KB) - Small changes in Wnt signalling

Chromosomes, positions please! - pp388 - 390

Ruth R. E. Williams & Amanda G. Fisher

doi:10.1038/ncb0503-388

Chromosome organization in the interphase nucleus is largely regarded to be non-random. However, the exact nature of this non-randomness and the mechanism for conveying positional information to daughter nuclei is a subject of intense debate, as two recent studies reveal.

Full Text - Chromosomes, positions please! | PDF (108 KB) - Chromosomes, positions please!

Memorable transcription - pp390 - 393

Bryan M. Turner

doi:10.1038/ncb0503-390

Experiments in the yeast Saccharomyces cerevisiae have shown that the enzyme Set1 preferentially targets the 5' coding regions of transcriptionally active genes, where it catalyses di- and tri-methylation of histone H3 Lys 4. This methylation mark is retained after transcription has subsided, suggesting that it provides a memory of recent transcription.

Full Text - Memorable transcription | PDF (113 KB) - Memorable transcription

Cell of the month: Actin stress fibres in MDCK cyst grown in collagen gel - p394

Wei Yu

doi:10.1038/ncb0503-394

Full Text - Cell of the month: Actin stress fibres in MDCK cyst grown in collagen gel | PDF (2,199 KB) - Cell of the month: Actin stress fibres in MDCK cyst grown in collagen gel

Chromatin and transcription: histones continue to make their marks - pp395 - 399

Mariela Jaskelioff & Craig L. Peterson

doi:10.1038/ncb0503-395

Chromatin architecture is modulated by a large number of enzymes, resulting in the proper regulation of transcription, replication, cell cycle progression, DNA repair, recombination and chromosome segregation. The structure, regulation and coordination of these enzymatic activities were the main topics of discussion at The Enzymology of Chromatin and Transcription Keystone Symposium held in Santa Fe, NM (March 10–16, 2003).

Full Text - Chromatin and transcription: histones continue to make their marks | PDF (149 KB) - Chromatin and transcription: histones continue to make their marks

Teaching genomics, proteomics and bioinformatics - p400

Sam Hanash reviews Discovering genomics, proteomics and bioinformatics by A. Malcolm Campbell & Laurie J. Heyer

doi:10.1038/ncb0503-400

Full Text - Teaching genomics, proteomics and bioinformatics | PDF (299 KB) - Teaching genomics, proteomics and bioinformatics

Chk2 activates E2F-1 in response to DNA damage - pp401 - 409

Craig Stevens, Linda Smith & Nicholas B. La Thangue

doi:10.1038/ncb974

Abstract - | Full Text - Chk2 activates E2F-1 in response to DNA damage | PDF (2,047 KB) - Chk2 activates E2F-1 in response to DNA damage | Supplementary information

Distinct endocytic pathways regulate TGF- receptor signalling and turnover - pp410 - 421

Gianni M. Di Guglielmo, Christine Le Roy, Anne F. Goodfellow & Jeffrey L. Wrana

doi:10.1038/ncb975

Abstract - | Full Text - Distinct endocytic pathways regulate TGF- receptor signalling and turnover | PDF (16,813 KB) - Distinct endocytic pathways regulate TGF- receptor signalling and turnover | Supplementary information

See also: News and Views by Felberbaum-Corti et al.

Progressive hearing loss in mice lacking the cyclin-dependent kinase inhibitor Ink4d - pp422 - 426

Ping Chen, Frederique Zindy, Caroline Abdala, Feng Liu, Xiankui Li, Martine F. Roussel & Neil Segil

doi:10.1038/ncb976

First Paragraph - | Full Text - Progressive hearing loss in mice lacking the cyclin-dependent kinase inhibitor Ink4d | PDF (2,871 KB) - Progressive hearing loss in mice lacking the cyclin-dependent kinase inhibitor Ink4d

See also: News and Views by Baumgartner & Harper

Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1 - pp427 - 432

Atsuo Sasaki, Takaharu Taketomi, Reiko Kato, Kazuko Saeki, Atsushi Nonami, Mika Sasaki, Masamitsu Kuriyama, Naoaki Saito, Masabumi Shibuya & Akihiko Yoshimura

doi:10.1038/ncb978

First Paragraph - | Full Text - Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1 | PDF (2,264 KB) - Mammalian Sprouty4 suppresses Ras-independent ERK activation by binding to Raf1 | Supplementary information

See also: News and Views by Christofori

Translokin is an intracellular mediator of FGF-2 trafficking - pp433 - 439

Carine Bossard, Henrik Laurell, Loïc Van den Berghe, Sylvain Meunier, Catherine Zanibellato & Hervé Prats

doi:10.1038/ncb979

First Paragraph - | Full Text - Translokin is an intracellular mediator of FGF-2 trafficking | PDF (2,010 KB) - Translokin is an intracellular mediator of FGF-2 trafficking | Supplementary information

Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum - pp440 - 446

Wihelma Echevarría, M. Fatima Leite, Mateus T. Guerra, Warren R. Zipfel & Michael H. Nathanson

doi:10.1038/ncb980

First Paragraph - | Full Text - Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum | PDF (2,904 KB) - Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum | Supplementary information

EGFR activation coupled to inhibition of tyrosine phosphatases causes lateral signal propagation - pp447 - 453

Andrew R. Reynolds, Christian Tischer, Peter J. Verveer, Oliver Rocks & Philippe I. H. Bastiaens

doi:10.1038/ncb981

First Paragraph - | Full Text - EGFR activation coupled to inhibition of tyrosine phosphatases causes lateral signal propagation | PDF (1,763 KB) - EGFR activation coupled to inhibition of tyrosine phosphatases causes lateral signal propagation | Supplementary information

The pathogenic peroxin Pex26p recruits the Pex1p–Pex6p AAA ATPase complexes to peroxisomes - pp454 - 460

Naomi Matsumoto, Shigehiko Tamura & Yukio Fujiki

doi:10.1038/ncb982

First Paragraph - | Full Text - The pathogenic peroxin Pex26p recruits the Pex1p–Pex6p AAA ATPase complexes to peroxisomes | PDF (1,769 KB) - The pathogenic peroxin Pex26p recruits the Pex1p–Pex6p AAA ATPase complexes to peroxisomes

Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation - pp461 - 466

Kaisa Haglund, Sara Sigismund, Simona Polo, Iwona Szymkiewicz, Pier Paolo Di Fiore & Ivan Dikic

doi:10.1038/ncb983

First Paragraph - | Full Text - Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation | PDF (1,612 KB) - Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation | Supplementary information

Degradation of DIAP1 by the N-end rule pathway is essential for regulating apoptosis - pp467 - 473

Mark Ditzel, Rebecca Wilson, Tencho Tenev, Anna Zachariou, Angela Paul, Emma Deas & Pascal Meier

doi:10.1038/ncb984

First Paragraph - | Full Text - Degradation of DIAP1 by the N-end rule pathway is essential for regulating apoptosis | PDF (2,567 KB) - Degradation of DIAP1 by the N-end rule pathway is essential for regulating apoptosis | Supplementary information

See also: News and Views by Varshavsky

Telomerase modulates expression of growth-controlling genes and enhances cell proliferation - pp474 - 479

Laura L. Smith, Hilary A. Coller & James M. Roberts

doi:10.1038/ncb985

First Paragraph - | Full Text - Telomerase modulates expression of growth-controlling genes and enhances cell proliferation | PDF (1,284 KB) - Telomerase modulates expression of growth-controlling genes and enhances cell proliferation | Supplementary information

Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I - pp480 - 485

Rosemary K. Clyne, Vittorio L. Katis, Lea Jessop, Kirsten R. Benjamin, Ira Herskowitz, Michael Lichten & Kim Nasmyth

doi:10.1038/ncb977

First Paragraph - | Full Text - Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I | PDF (1,374 KB) - Polo-like kinase Cdc5 promotes chiasmata formation and cosegregation of sister centromeres at meiosis I | Supplementary information

See also: News and Views by Watanabe

Reconstitution of -secretase activity - pp486 - 488

Dieter Edbauer, Edith Winkler, Joerg T. Regula, Brigitte Pesold, Harald Steiner & Christian Haass

doi:10.1038/ncb960

Abstract - | Full Text - Reconstitution of -secretase activity | PDF (235 KB) - Reconstitution of -secretase activity | Supplementary information

Erratum - p488

doi:10.1038/ncb986

Full Text - Erratum | PDF (51 KB) - Erratum

Erratum: Exocytosis of single chromaffin granules in cell-free inside-out membrane patches - p488

doi:10.1038/ncb987

Full Text - Erratum: Exocytosis of single chromaffin granules in cell-free inside-out membrane patches | PDF (51 KB) - Erratum: Exocytosis of single chromaffin granules in cell-free inside-out membrane patches

Erratum: A new link between the c-Abl tyrosine kinase and phosphoinositide signalling through PLC-1 - p488

doi:10.1038/ncb988

Full Text - Erratum: A new link between the c-Abl tyrosine kinase and phosphoinositide signalling through PLC-1 | PDF (51 KB) - Erratum: A new link between the c-Abl tyrosine kinase and phosphoinositide signalling through PLC-1