Abstract
Aberrant cellular responses to pro-inflammatory cytokines, such as IFN-γ, are pathogenic features in many chronic inflammatory diseases. A variety of feedback regulatory pathways have evolved to prevent an inappropriate cellular reaction to these pro-inflammatory cytokines. CX3CL1 is a unique chemokine and plays an important role in chronic liver diseases. We report here that IFN-γ stimulation induces a transient CX3CL1 production in liver epithelial cells (i.e., hepatocytes and biliary epithelial cells). This transient CX3CL1 production is accompanied with a destabilization of CX3CL1 mRNA associated with the induction of the KH-type splicing regulatory protein (KSRP). IFN-γ treatment of liver epithelial cells decreases expression level of miR-27b, a miRNA that targets the 3′ untranslated region of KSRP mRNA resulting in translational suppression. Induction of KSRP following IFN-γ stimulation depends on the downregulation of miR-27b. Functional manipulation of KSRP or miR-27b caused reciprocal alterations in CX3CL1 mRNA stability in liver epithelial cells. Moreover, transfection of miR-27b precursor influences CX3CL1-associated chemotaxis effects of biliary epithelial cells to Jurkat T cells. These findings suggest that miR-27b-mediated post-transcriptional suppression controls the expression of KSRP in liver epithelial cells and upregulation of KSRP destabilizes CX3CL1 mRNA, providing fine-tuning of cellular inflammatory reactions in response to IFN-γ stimulation.
Similar content being viewed by others
Introduction
The inflammatory response is a double-edged sword, as excessive inflammation itself can exacerbate tissue damage1,2. Chronic inflammation and cellular injury are common pathogenic features for a variety of important hepatobiliary diseases, such as chronic type C hepatitis3. Persistent inflammation in the liver of patients with these diseases is usually accompanied with increased expression of multiple inflammatory mediators, including inflammatory cytokines/chemokines4. To limit the undesirable consequences of excessive inflammation, liver epithelial cells (i.e., hepatocytes and biliary epithelial cells) have developed regulatory strategies to control the initiation and resolution of inflammatory response5,6. The coordinated expression of various components of cellular inflammatory response involves multiple steps that determine rates of gene transcription, translation and mRNA decay6,7. Although transcription is an essential first step in the regulation of gene expression, post-transcriptional regulation of translation and mRNA decay is key to control protein synthesis from transcribed mRNAs6. It is now apparent that 3′-untranslated region (3′UTR)-mediated RNA stability and translational activation play an important regulatory role in the post-transcriptional regulation of protein synthesis7,8. Nevertheless, the role for 3′UTR-mediated post-transcriptional regulation in the coordination of liver epithelial cell inflammatory responses remains to be defined.
Several RNA-binding proteins, including the KH-type splicing regulatory protein (KSRP, also known as KHSRP), tristetraprolin (TTP) and Hu antigen R (HuR), recognize AU-rich elements (AREs) within the 3′UTRs of mRNAs and control their half-life time in the cytoplasm7,8,9. In this regard, KSRP interacts with these mRNAs that have the AREs within their 3′UTRs and is a key mediator of mRNA decay10. Some KSRP-regulated mRNAs code proteins are key to cellular inflammatory response, including mRNAs for inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2)11. The 3′UTR is also critical to miRNA-mediated post-transcriptional gene regulation. In mammalian cells, miRNAs identify targets based on complementarity between each miRNA and 3′UTR of target mRNAs, resulting in mRNA cleavage and/or translational suppression12.
The chemokine CX3CL1 (also known as fractalkine) is a unique member of the CX3C family; and it binds only to the unique ligand of its receptor, CX3CR113. Unlike other chemokines, CX3CL1 is expressed as a membrane-bound form (95–100 kDa) and can also be shed in a soluble chemotactic form (60–80 kDa)13,14. Membrane-bound CX3CL1 is known to function as an adhesion molecule to interact with immune cells that express CX3CR1, including CD4 + and CD8 + T-cells, NK cells and monocytes15. Recent evidence shows that increased level of CX3CL1 in the liver is associated with severe inflammatory liver diseases16. In our previous studies, we demonstrated that induction of CX3CL1 expression in biliary epithelial cells upon microbial challenge involves downregulation of miR-424 and miR-50317. Histone deacetylases and NF-ĸB signaling coordinate downregulation of the mir-424-503 gene and promote biliary mucosal defense through CX3CL1 induction in biliary epithelial cells17. Using in vitro and in vivo models of biliary Cryptosporidiosis, we found that KSRP is a target of miR-27b in biliary epithelial cells; and post-transcriptional suppression of KSRP by miR-27b stabilizes iNOS mRNA and facilitates TLR4-mediated biliary epithelial defense against Cryptosporidium parvum infection18.
IFN-γ, a type II interferon with important immunomodulatory properties, plays a critical role in mediating liver inflammatory responses. While IFN-γ is key to innate and adaptive immunity against viral and intracellular bacterial infection in the liver, uncontrolled IFN-γ signaling may be pathogenic, contributing to the pathogenesis of chronic autoimmune and inflammatory hepatobiliary diseases19. IFN-γ triggers transactivation of many inflammatory genes and increases their expression in liver cells19,20. In this report, we investigated the expression of KSRP in liver epithelial cells following IFN-γ stimulation, its relationship to miR-27b-mediated translational suppression and finally, its impact on cellular inflammatory responses in the liver. Our findings implicate that relief of miR-27b-mediated post-transcriptional suppression of KSRP destabilizes CX3CL1 mRNA in liver epithelial cells, a process that may provide fine-tuning of liver epithelial inflammatory reactions to IFN-γ.
Results
IFN-γ stimulation induces a transient CX3CL1 expression
We first assessed expression of CX3CL1 at the mRNA level and protein level in cultured hepatocytes (AML12) and biliary epithelial cells (H69 and 603B) in response to IFN-γ stimulation. An increased CX3CL1 mRNA level was detected in AML12 cells following IFN-γ treatment for 8 h (Fig. 1A). Interestingly, CX3CL1 mRNA level started to decline at 8 h and continued to decrease till 48 h following IFN-γ stimulation (Fig. 1A). In H69 and 603B cells, levels of CX3CL1 mRNA started to increase at 4 h and began to decline after 12 h following IFN-γ treatment (Fig. 1A). The CX3CL1 protein content in AML12 and 603B cell lyses was measured by ELISA (Fig. 1B). CX3CL1 protein content in AML12 cell lyses started to increase after IFN-γ treatment for 4 h and reached to the peak at 12 h, then declined after 12 h following IFN-γ treated (Fig. 1B). Similarly, we found that the CX3CL1 protein level in 603B cells was increased at 4 h and started to decrease at 24 h after IFN-γ treatment (Fig. 1B). These data suggested that IFN-γ stimulation induces only a transient CX3CL1 expression in liver epithelial cells.
IFN-γ stimulation decreases CX3CL1 mRNA stability
To assess the potential effects of IFN-γ stimulation on the stability of CX3CL1 mRNA, AML12, 603B and H69 cells were treated with IFN-γ for 24 h. Cells were then treated with actinomycin D in the presence of IFN-γ for additional 2 h, followed by measurement of CX3CL1 mRNA by real-time PCR. After actinomycin D treatment, the decay of CX3CL1 mRNA in the IFN-γ-treated cells was faster, compared with that for the untreated cells (Fig. 2A–C). To test whether IFN-γ treatment influence mRNA stability of other pro-inflammatory effectors, we tested mRNA stability of COX-2 and iNOS in AML12, H69 and 603B cells following IFN-γ stimulation. Cells pre-treated with IFN-γ also displayed decreased mRNA stability of COX-2 and iNOS compared with the untreated cells (Fig. S1A–S1C).
IFN-γ stimulation induces expression of KSRP protein without change in KSRP mRNA in vitro and in vivo
Destabilization of CX3CL1 mRNA in cells following IFN-γ stimulation, along with the mRNAs of COX-2 and iNOS, suggests to us that a similar mechanism may be involved. KSRP is a RNA binding protein, which can recognize AREs within the 3′UTRs of mRNAs of COX-2, iNOS and control their half-life time in the cytoplasm11. The 3′UTR of CX3CL1 mRNA also possesses the ARE sequence21. We then assessed KSRP expression at the mRNA level and protein level in hepatocytes and biliary epithelial cells in response to IFN-γ stimulation. The expression of KSRP protein started to increase in AML12 cells after IFN-γ treated for 8 h and continue to increase till 24 h following IFN-γ stimulation (Fig. 3A). The increased of KSRP at protein level was also detected in H69 and 603B cells (Fig. S2A). A dose-dependent increase of KSRP protein expression was detected by Western blot in H69 and 603B cells following IFN-γ stimulation (Fig. S2A). Interestingly, no significant change of KSRP mRNA levels was found by real-time PCR in IFN-γ-treated AML12 cells (Fig. 3B), H69 cells, 603B cells and primary hepatocytes isolated from mice (Fig. S2B). Consistent with in vitro results, a significant increase of KSRP protein content was detected in the liver from mice after IFN-γ i.p. injection for 24 h by immunohistochemistry (Fig. 3C). Consistent with results of previous studies22, we observed that KSRP protein was strongly enriched in the nuclei in hepatocytes and biliary epithelial cells after IFN-γ i.p. injection (Fig. 3C).
IFN-γ stimulation decreases miR-27b expression in vitro and in vivo
Given the detection of an increased amount of KSRP protein without a significant change in its mRNA levels in cells following IFN-γ stimulation, we expected that miRNA-mediated post-transcriptional regulation may be involved. We previously described an altered expression profile of mature miRNAs in human biliary epithelial cells following IFN-γ stimulation23. Of these miRNAs expressed in H69 cells, expression of miR-27b showed a tendency to decrease (0.05 < p < 0.10) after exposure to IFN-γ for 8 h by the miRCURYTM LNA human microRNAs assays (Fig. 4A). The expression of mature miR-27b was showed decreased in AML12 cells after IFN-γ treatment for 8 h and 12 h by real-time PCR (Fig. 4B). A decrease of miR-27b expression was also observed in H69 and 603B cells following IFN-γ stimulation for 8 h (Fig. S3). Our Northern blot analysis further confirmed the decrease of mature miR-27b in AML12 cells following IFN-γ stimulation for 8 h (Fig. 4C). Of note, pre-miR-27b was not obvious in the Northern gel, suggesting a low level of the primary transcript of miR-27b in the cells. Moreover, the expression of miR-27b was decreased in primary mouse hepatocytes after IFN-γ treatment for 8 h (Fig. 4D). Our in situ hybridization demonstrated a predominant cytoplasmic labeling for mature miR-27b in both hepatocytes and biliary epithelial cells of the liver tissues from non-IFN-γ-treated mice (Fig. 4E). Nuclear labeling was not obvious, representing a low level of primary transcript in the nuclei and consistent with the results from our Northern Blot analysis on AML12 cells. A lower level of miR-27b expression signal was detected in hepatocytes and biliary epithelial cells in IFN-γ-injected mice by in situ hybridization, compared to the untreated mice (Fig. 4E).
KSRP regulates the stabilization of CX3CL1 mRNA through targeting ARE
Both human and mouse CX3CL1 mRNA 3′UTR contain a single UUAUUUAUU nonamer21. To determine whether KSRP plays a role in the regulation of CX3CL1 expression through targeting ARE, we used two constructs containing the luciferase cDNA fused to the full-length CX3CL1 3′UTR (pcDNA3-luc-CX3CL1-FL-ARE) and the full-length CX3CL1 3′UTR with deletion of ARE (pcDNA3-luc-CX3CL1-FL-△ARE). Two constructs containing the truncated 3′UTR containing a UUAUUUAUU nonamer (pcDNA3-luc-CX3CL1-ARE) and deletion of the UUAUUUAUU nonamer of 3′UTR (pcDNA3-luc-CX3CL1-▵ARE) were also used in the experiments. H69 cells were transfected with these constructs with pcDNA3-flag-KSRP (overexpression of KSRP) by assessment of luciferase activity 24 h after transfection. The transfection efficiency for the luciferase plasmids in H69 cells was around 50% and therefore, luciferase activity was normalized to the expression of the control β-gal construct. Overexpression KSRP in H69 cells resulted in a significant decrease in luciferase activity of pcDNA3-luc-CX3CL1-FL-ARE and pcDNA3-luc-CX3CL1-ARE, compared with that in the control (Fig. 5A and S4, respectively). In cells transfected with the construct deletion of the ARE nonamer, the associated luciferase activity was not changed, compared with the control (Fig. 5A and S4, respectively). To further identify whether KSRP regulates CX3CL1 mRNA stability, we measured CX3CL1 mRNA stability in KSRP knockdown cells. Transfection of 603B cells with a mouse KSRP shRNA construct or treatment of H69 cells with a siRNA to human KSRP significantly inhibited the expression of KSRP mRNA (Fig. S5). As shown in Fig. 5B, 603B cells stably expressing KSRP shRNA displayed a significant increase in CX3CL1 mRNA stability. The increased mRNA stability of CX3CL1 was also detected in H69 cells transfected with KSRP siRNA compared to the control cells (Fig. 5C).
miR-27b regulates the stabilization of CX3CL1 mRNA in an ARE-dependent manner
Our previous study suggested that miR-27b can target KSRP in epithelial cells18. We then asked whether miR-27b regulates CX3CL1 mRNA stability. CX3CL1 mRNA expression was measured in AML12 cells transfected with anti-miR-27b or miR-27b precursor. miR-27b precursor increased CX3CL1 mRNA expression compared with the control (Fig. 6A). In contrast, anti-miR-27b showed a significant decrease in the expression of CX3CL1 mRNA (Fig. 6A). To determine whether miR-27b regulates CX3CL1 expression through targeting ARE in 3′UTR, H69 cells were co-transfected with constructs containing CX3CL1 3′UTR (pcDNA3-luc-CX3CL1-FL-ARE or pcDNA3-luc-CX3CL1-ARE) or the CX3CL1 3′UTR with deletion of ARE (pcDNA3-luc-CX3CL1-FL-△ARE or pcDNA3-luc-CX3CL1-△ARE ) together with the miR-27b precursor. miR-27b precursor transfection showed a significant increase of luciferase activity in H69 cells transfected with constructs containing CX3CL1 3′UTR with ARE (pcDNA3-luc-CX3CL1-FL-ARE and pcDNA3-luc-CX3CL1-ARE), compared to the control (Fig. 6B and S6, respectively). Inhibition of associated luciferase activity was not detected in cells transfected with the ARE-deleted construct, compared to the control after miR-27b precursor transfection (Fig. 6B and S6, respectively). Furthermore, CX3CL1 mRNA stability was measuring in 603B cells transfected with miR-27b precursor, comparing with the control (Fig. 6C). As shown in Fig. 6C, 603B cells transfected with miR-27b precursor displayed a significant increase in CX3CL1 mRNA stability.
IFN-γ stimulation destabilized CX3CL1 mRNA through targeting ARE
Given the downregulation of miR-27b after IFN-γ stimulation and the impact of miR-27b on CX3CL1 mRNA stability through targeting ARE, we speculated that IFN-γ treatment should affect ARE-mediated post-transcription of CX3CL1. To test this possibility, H69 cells were transfected with constructs containing CX3CL1 3′UTR with ARE (pcDNA3-luc-CX3CL1-FL-∆ARE or pcDNA3-luc-CX3CL1-∆ARE) or the CX3CL1 3′UTR with deletion of ARE (pcDNA3-luc-CX3CL1-FL-∆ARE or pcDNA3-luc-CX3CL1-∆ARE) and then exposed to IFN-γ stimulation for 24 h. IFN-γ treatment decreased the luciferase activity in H69 cells transfected constructs containing CX3CL1 3′UTR with ARE, but did not alter the luciferase activity in cells transfected with constructs containing deletion of ARE (Fig. 7A and S7, respectively). In addition, AML12 cells were transfected with miR-27b precursor for 24 h and then exposed to IFN-γ for another 24 h, followed by Western blot for KSRP protein. miR-27b precursor inhibited the upregulation of KSRP protein in AML12 cells induced by IFN-γ stimulation (Fig. 7B). Taken together, the above data suggest that miR-27b regulates the stabilization of CX3CL1 mRNA through targeting ARE following IFN-γ stimulation.
miR-27b regulates chemotaxis effects of CX3CL1 to Jurkat cells
Membrane-bound CX3CL1 has been reported to function as an adhesion molecule to interact with CX3CR1-positive immune cells, including T-cells, NK cells and monocytes13. We then tested the role for CX3CL1-induced chemotaxis activity in liver epithelial cells. Because of the potential histocompatibility between different species, we chose to use a co-culture system employing two human cell lines: Jurkat cells in 8 nm membrane inserts co-cultured with IFN-γ-treated H69 cells. The JKT is a cancer cell line of leukemia with certain changes of the original T cell characteristics24. However, it expresses CX3CR1 and has previously been used for chemotaxis assay25. H69 cells were treated with IFN-γ after miR-27b precursor transfection in the presence or absence of a neutralizing Ab to CX3CL1. After 2.5 h of incubation, Jurkat cells migrated through the membrane into the H69 medium pool were counted. A significant increase of Jurkat cell migration was detected after IFN-γ treatment (Fig. 8). Neutralizing Ab to CX3CL1 showed a significant inhibitory effect of Jurkat cell migration induced by IFN-γ. Moreover, miR-27b precursor transfection showed a significant increase of Jurkat cell migration after exposed to IFN-γ (Fig. 8). IFN-γ-stimulated Jurkat cell migration was partially blocked after miR-27b precursor transfection of H69 cells, suggesting that miR-27b regulates chemotaxis effects of CX3CL1-expressing H69 cells to Jurkat cells.
Discussion
Our findings reveal a novel role for miR-27b in the negative regulation of immune reactions in liver epithelial cells in response to IFN-γ stimulation. We found that IFN-γ stimulation decreased miR-27b expression and increased KSRP protein content without changing its mRNA level in liver epithelial cells in vitro and in vivo. Besides these traditional KSRP-regulated ARE-containing mRNAs of iNOS and COX-218, upregulation of KSRP destabilized CX3CL1 mRNA through interacting with the ARE within its 3′UTRs in liver epithelial cells. Through targeting KSRP, miR-27b regulated the stabilization of CX3CL1 mRNA. Consequently, downregulation of miR-27b following IFN-γ stimulation resulted in KSRP induction, providing negative feedback regulation of cytokine/chemokine expression in liver epithelial cells.
Persistent inflammation is mediated by increased expression of multiple pro-inflammatory mediators in most chronic inflammatory diseases. The coordinated expression of the protein components of a complex functional program such as the inflammatory response involves both transcriptional and post-transcriptional mechanisms6. A central part of post-transcriptional modulation of gene expression is mediated by the regulation of mRNA stability. A tight control of mRNA stability permits rapid changes in the levels of mRNAs and provides a mechanism for prompt termination of protein production7,8. The mRNAs encoding subsets of inflammation-relative proteins potentially injurious to host tissues, such as iNOS and COX-2, contain regulatory elements that direct their degradation and translational repression to protect against pathological overexpression9,10,18. Our study provides a new mechanism of controlling excessive inflammation through downregulation of miR-27b to modulate KSRP expression.
Previous study indicated that CX3CL1 plays an important role in chronic inflammatory diseases26. The expression of CX3CL1 is up-regulated in chronic liver diseases such as chronic hepatitis C16. Transcriptional and post-transcriptional regulations were both involved in the regulation of CX3CL1 expression21. The 3′UTR of CX3CL1 mRNA contains AREs and HuR has been shown to modulate CX3CL1 mRNA stability by binding to the AREs within the 3′UTR and to control its half-life time in the cytoplasm21. In this study, for the first time, we demonstrated that CX3CL1 mRNA stability is directly regulated by KSRP through its interaction with the AREs within CX3CL1 mRNA 3′UTR. This post-transcriptional mechanism is involved in the transient expression of CX3CL1 in liver epithelial cells in response to IFN-γ stimulation.
KSRP is a single-strand nucleic acid binding protein and known to be one of the most important mRNA destabilizing proteins22. Some of these KSRP-regulated mRNAs code proteins with important immune functions, such as iNOS and COX-211,27 and now, include CX3CL1. Given its critical role in regulating inflammatory responses, KSRP should be tightly controlled in the cells at physiological conditions. Previous studies demonstrated that at least two signaling pathways, the MAPK p38 and the Akt/PKB, can target KSRP and modulate ARE-mediated mRNA decay28. In our previous studies, we reported that miR-27b targets KSRP 3′UTR resulting in translational suppression of KSRP in biliary epithelial cells18. Upregulation of miR-27b suppresses KSRP expression, facilitating NO production through stabilization of iNOS in biliary epithelial cells against C. parvum infection18. Here, our findings indicate that IFN-γ stimulation downregulates miR-27b expression in liver epithelial cells, providing negative feedback regulation of cytokine/chemokine expression through KSRP induction in the liver.
miRNA-mediated post-transcriptional mechanisms have recently been demonstrated to play an important role in regulation of epithelial innate immunity29. Recent evidence showing altered miRNA expression in various inflammation cells suggested their involvement in inflammatory diseases2. Importantly, alterations in miRNA expression following stimulation are controlled by activation of intracellular signaling pathway network. We previously demonstrated that C. parvum infection and LPS stimulation activates liver epithelial cell TLR4/NF-ĸB signaling, resulting in alterations in expression of a panel of miRNAs18,30. In this regard, how IFN-γ stimulation suppresses the expression of miR-27b is still unclear and merits further investigation. Our findings cannot exclude the possibility of miRNA targeting of CX3CL1 mRNA 3′UTR, resulting in post-transcriptional regulation of CX3CL1 expression in liver epithelial cells.
In summary, our study provides evidence that miR-27b regulates the mRNA stability of CX3CL1 to maintain cellular homeostasis through targeting KSRP in liver epithelial cells. Post-transcriptional regulation of CX3CL1 stability by KSRP through miRNAs may represent a new mechanism by which liver epithelial cells maintain homeostasis during inflammation, relevant to fine-tuning of cellular inflammatory responses in general.
Materials and Methods
Ethics statement
Male C57BL/6J mice, 6 to 8 week old, weighing 22–25 g were used. Animal protocols were approved by the Animal Care and Use Committee of Wuhan University and all experiments were performed in accordance with the guidelines and regulations of the university.
All surgeries were performed under ketamine (100 mg/kg body weight, i.p) and xylazine (10 mg/kg body weight, i.p.) anesthesia and all efforts were made to minimize suffering to the animals.
Cell lines
H69 cells (a gift of Dr. D. Jefferson, Tufts University) are SV40 transformed normal human biliary epithelial cells originally derived from liver harvested for transplant. These cells continue to express biliary epithelial cell markers, including cytokeratin 19, gamma glutamyl transpeptidase and ion transporters consistent with biliary function and have been extensively characterized31. The 603B cells are immortalized normal mouse biliary epithelial cells (a gift from Y. Ueno, Tohoku University School of Medicine, Sendai, Japan). AML12 is a murine hepatocyte cell line and was obtained from the American Type Culture Collection (CD1 strain, line MT42). Primary murine hepatocytes were purchased from Celsis (Chicago, USA) and cultured according to the instructions from the company.
Plasmids and reagents
The expression vector for KSRP (pcDNA3-Flag-KSRP) carrying insertion of the coding regions of KSRP is a kind gift from Dr. Ching-Yi Chen (University of Alabama, Birmingham, AL). A KSRP shRNA construct was prepared in the vector pRNA-U6.1/hygro. The target sequence for KSRP was based on sequences within the KSRP coding region (GGACAGTTTCACGACAACG). Human KSRP siRNA purchased from Santa Cruz Biotechnology. The vectors containing human CX3CL1 ARE of 3′UTR or CX3CL1 3′UTR with deletion of AU-rich element (pcDNA3-luc-CX3CL1-FL-ARE;pcDNA3-luc-CX3CL1–ARE; pcDNA3-luc-CX3CL1-FL-△ARE and pcDNA3-luc-CX3CL1-△ARE) are kind gifts from Dr. Matsumiya (Hirosaki University Graduate School of Medicine, Hirosaki City, Japan). Actinomycin D (10 μg/ml) was purchased from Fisher Scientific (Pittsburgh, PA). At the utilized concentrations, no cytotoxic effects of any of the chemicals were observed on AML12, H69 and 603B cells (data not shown).
Real-time PCR
For real-time PCR analysis of mature miRNAs, total RNAs were extracted using the mirVana™ miRNA Isolation kit (Applied Biosystems). An amount of 0.05 μg total RNAs was reverse-transcribed by using the Taqman MicroRNA Reverse Transcription Kit (Applied Biosystems). Comparative real-time PCR was performed in triplicate using Taqman Universal PCR Master Mix (Applied Biosystems) on the Applied Biosystems 7500 FAST real-time PCR System. Mature miR-27b primers and probes were obtained from Applied Biosystems. Normalization was performed by using RNU6B primers and probes. Relative expression was calculated by using the comparative CT method30,32.
For analysis of mRNA, total RNA was isolated from cells with Trizol reagent (Applied Biosystems). RNAs were treated with DNA-freeTM Kit (Applied Biosystems) to remove any remaining DNA. Comparative real-time PCR was performed by using the SYBR Green PCR Master Mix (Applied Biosystems). Specific primers for mRNAs were listed in Table S1. All reactions were run in triplicate. The Ct values were analyzed using the comparative Ct (△△Ct) method and the amount of target was obtained by normalizing to the endogenous reference and relative to the control (non-treated cells)32.
ELISA
For the measurement of CX3CL1 production by AML12 and 603B cells in six well culture plates were stimulated with IFN-γ (10 ng/ml) for up to 48 h. Cells were collected with lysis buffer at different time point following IFN-γ stimulation. The level of CX3CL1 in the cell lyses was determined using a Quantikine ELISA kit (R&D Systems).
Western blot
Whole cell lysates were obtained from cells with MPER mammalian protein extraction reagent (Pierce) containing several protease inhibitors (1 mM PMSF, 10 μg/ml leupeptin and 2 μg/ml pepstatin). Cell lysates were then loaded in SDS-PAGE gel to separate proteins and transferred to nitrocellulose membrane. Antibodies to KSRP (Bethyl Laboratories) and actin (Sigma-Aldrich) were used. Densitometric levels of Western blot signals were quantified and expressed as their ratio to actin18.
IFN-γ injection in vivo and immunohistochemistry for KSRP
The C57BL/6J mice (The Jacksons Laboratory) were used for the study, with the approval of the Animal Care and Use Committee of Wuhan University. Animals received treatment of IFN-γ (5 μg in 200 μl of saline) by intraperitoneal (i.p.) injection for 24 h, as previously reported33. Five animals from each group were sacrificed and liver tissues obtained for immunohistochemistry. Antibodies to KSRP (Bethyl Laboratories) were utilized.
RNA stability
Cells were stimulated by IFN-γ for 24 h or transfected with miR-27b precursor (30 nM) or KSRP siRNA (50 nM), then treated with IFN-γ (10 ng/ml) for another 2 h. Transcription was stopped by actinomycin D (10 μg/ml) and RNAs were prepared at various time points following actinomycin D treatment. Real-time PCR was then performed using 500 ng of template cDNA from the resultant RNA. Each sample was run in triplicate. The relative abundance of each mRNA was calculated using the ΔΔCt method and normalized to GAPDH (human) or actin (mouse). The relative amount of mRNA at 0 h following actinomycin D treatment was arbitrarily set to 1. Curve fittings of the resultant data were performed using Microsoft Excel and the half-lives of selected RNAs calculated, as previously reported30.
In situ hybridization
Paraffin tissue sections were deparaffinized and treated with 10 μg/ml proteinase K (Roche) at 37 °C for 10 min, as reported18. After washing with PBS, slides were incubated with the hybridization buffer (50% formamide, 100 μg/ml salmon sperm DNA, 200 μg/ml yeast tRNA, 600 mM NaCL, 1 × Denhardt’s solution, 0.25% SDS, 1 mM EDTA) at 42 °C for 1 h. Slides were then hybridized with 20 nM DIG-labelled miR-27b probe (Exiqon) diluted in the hybridization buffer at 42 °C overnight. Slides were incubated with anti-DIG-POD Fab fragments (Roche) at 4 °C overnight and miR-27b was visualized in a staining reaction with Renaissance Tyramide Signal Amplification Fluorescence Systems (PerkinElmer). For all experiments, a negative control (i.e. staining without miR-27b probe) was included18.
Transfection and Reporter assay
Transient transfections of H69 cells were accomplished by plating cells at a density of 1 × 105cells per well of a 24-well culture plate 24 h before transfection. 250 ng of CX3CL1 3′UTR or its mutants and 250 ng pCMV-β-Gal were co-transfected using 1 μl of Lipofectamine 2000 transfection reagent (invitrogen) following the vendor’s instructions. The cells were incubated for 24 h and then analyzed for the reporter activity. Cells were harvested in Reporter lysis buffer (Progema) and the luciferase activity was determined by Luciferase assay system (Progema). Luciferase activities were normalized by transfection efficiency by β-gal activity measured using a β-gal reporter gene assay chemiluminescence kit (Roche, Basel, Switzerland). Data were presented as mean ± SD of at least three independent experiments.
Chemotaxis assay
H69 and Jurkat cells were cocultured as previously reported23. Briefly, H69 cells were seeded at 1 × 105 cells/well in 24-well plates, transfected with miR-27b precursor (30 nM) or pre-control (30 nM) for 48 h, then exposed to IFN-γ for 24 h in the presence or absence of a neutralizing Ab to CX3CL1 (Roche, Basel, Switzerland). H69 cells were then co-cultured with Jurkat cells grown in 8 nm membrane inserts (2 × 105) as previously reported25. After 2.5 h of co-culture, Jurkat cells migrated through the insert membrane into the H69 medium pool were collected. The number of Jurkat cells was counted under a microscope as previously reported. Normal rabbit IgG (Santa Cruz) was used as a negative control.
Additional Information
How to cite this article: Xia, Z. et al. Upregulation of KSRP by miR-27b provides IFN-γ-induced post-transcriptional regulation of CX3CL1 in liver epithelial cells. Sci. Rep. 5, 17590; doi: 10.1038/srep17590 (2015).
References
Brenner, C., Galluzzi, L., Kepp, O. & Kroemer, G. Decoding cell death signals in liver inflammation. J. Hepatol. 59, 583–594 (2013).
O’Connell, R. M., Rao, D. S. & Baltimore, D. microRNA regulation of inflammatory responses. Annu. Rev. Immunol. 30, 295–312 (2012).
Bowen, D. G. & Walker, C. M. Adaptive immune responses in acute and chronic hepatitis C virus infection. Nature. 436, 946–952 (2005).
Fahey, S., Dempsey, E. & Long, A. The role of chemokines in acute and chronic hepatitis C infection. Cell. Mol. Immunol. 11, 25–40 (2014).
Seki, E. & Schwabe, R. F. Hepatic inflammation and fibrosis: functional links and key pathways. Hepatology. 61, 1066–1079 (2015).
Stoecklin, G. & Anderson, P. Posttranscriptional mechanisms regulating the inflammatory response. Adv. Immunol. 89, 1–37 (2006).
Anderson, P. Post-transcriptional control of cytokine production. Nat Immunol. 9, 353–359 (2008).
Chen, C. Y. & Shyu, A. B. AU-rich elements: characterization and importance in mRNA degradation. Trends. Biochem. Sci. 20, 465–470 (1995).
Dean, J. L., Sully, G., Clark, A. R. & Saklatvala, J. The involvement of AU-rich element-binding proteins in p38 mitogen-activated protein kinase pathway-mediated mRNA stabilisation. Cell. signal. 16, 1113–1121 (2004).
Winzen, R. et al. Functional analysis of KSRP interaction with the AU-rich element of interleukin-8 and identification of inflammatory mRNA targets. Mol. Cell. Biol. 27, 8388–8400 (2007).
Linker, K. et al. Involvement of KSRP in the post-transcriptional regulation of human iNOS expression-complex interplay of KSRP with TTP and HuR. Nucleic Acids Res. 33, 4813–4827 (2005).
Bartel, D. P. MicroRNAs: genomics, biogenesis, mechanism and function. Cell. 116, 281–297 (2004).
Imai, T. et al. Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 91, 521–530 (1997).
Wasmuth, H. E., Tacke, F. & Trautwein, C. Chemokines in liver inflammation and fibrosis. Semin. Liver Dis. 30, 215–225 (2010).
Chakravorty, S. J. et al. Fractalkine expression on human renal tubular epithelial cells: potential role in mononuclear cell adhesion. Clin. Exp. Immunol. 129, 150–159 (2002).
Aoyama, T., Inokuchi, S., Brenner, D. A. & Seki, E. CX3CL1-CX3CR1 interaction prevents carbon tetrachloride-induced liver inflammation and fibrosis in mice. Hepatology. 52, 1390–1400 (2010).
Zhou, R. et al. Histone deacetylases and NF-kB signaling coordinate expression of CX3CL1 in epithelial cells in response to microbial challenge by suppressing miR-424 and miR-503. PLoS. One. 8, e65153 (2013).
Zhou, R., Gong, A. Y., Eischeid, A. N. & Chen, X. M. miR-27b targets KSRP to coordinate TLR4-mediated epithelial defense against Cryptosporidium parvum infection. PLoS. Pathog. 8, e1002702 (2012).
Horras, C. J., Lamb, C. L. & Mitchell, K. A. Regulation of hepatocyte fate by interferon-gamma. Cytokine. Growth. F. R. 22, 35–43 (2011).
Schoenborn, J. R. & Wilson, C. B. Regulation of Interferon‐γ During Innate and Adaptive Immune Responses. Adv. Immunol. 96, 41–101 (2007).
Matsumiya, T. et al. Characterization of synergistic induction of CX3CL1/fractalkine by TNF-alpha and IFN-gamma in vascular endothelial cells: an essential role for TNF-alpha in post-transcriptional regulation of CX3CL1. J. Immunol. 184, 4205–4214 (2010).
Briata, P. et al. KSRP, many functions for a single protein. Front. Biosci. 16, 1787–1796 (2011).
Gong, A.-Y. et al. MicroRNA-513 Regulates B7-H1 Translation and Is Involved in IFN-γ-Induced B7-H1 Expression in Cholangiocytes. J. Immunol. 182, 1325–1333. (2009).
Schneider, U., Schwenk, H. U. & Bornkamm G. Characterization of EBV-genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer. 19, 621–626. (1997)
Isse, K. et al. Fractalkine and CX3CR1 are involved in the recruitment of intraepithelial lymphocytes of intrahepatic bile ducts. Hepatology. 41, 506–516 (2005).
Jones, B. A., Beamer, M. & Ahmed, S. Fractalkine/CX3CL1: a potential new target for inflammatory diseases. Mol. Interv. 10, 263–270 (2010).
Ivanov, P. & Anderson, P. Post-transcriptional regulatory networks in immunity. Immunol. Rev. 253, 253–272 (2013).
Briata, P. et al. PI3K/AKT signaling determines a dynamic switch between distinct KSRP functions favoring skeletal myogenesis. Cell. Death. Differ. 19, 478–487 (2012).
Zhou, R., O’Hara, S. P. & Chen, X. M. MicroRNA regulation of innate immune responses in epithelial cells. Cell. Mol. Immunol. 8, 371–379 (2011).
Zhou, R. et al. NF-kappaB p65-dependent transactivation of miRNA genes following Cryptosporidium parvum infection stimulates epithelial cell immune responses. PLoS. Pathog. 5, e1000681 (2009).
Grubman, S. A. et al. Regulation of intracellular pH by immortalized human intrahepatic biliary epithelial cell lines. Am. J. Physiol. 266, G1060–1070 (1994).
Zhou, R. et al. Binding of NF-kappaB p65 subunit to the promoter elements is involved in LPS-induced transactivation of miRNA genes in human biliary epithelial cells. Nucleic Acids Res. 38, 3222–3232 (2010).
Claudia, L. et al. Differential Effects of Endogenous and Exogenous Interferong on Immunoglobulin E, Cellular Infiltration and Airway Responsiveness in a Murine Model of Allergic Asthma. Am. J. Respir. Cell. Mol. Biol. 19, 826–835 (1998).
Acknowledgements
We acknowledge funding by the National Natural Science Foundation of China (NSFC) (No. 81373132 and No. 31300744), Specialized Research Fund for the Doctoral Program of Higher Education of China (SRFDP) (No. 20130141120011) and the Fundamental Research Funds for the Central Universities.
Author information
Authors and Affiliations
Contributions
Z.J.X., Y.J.L. and R.Z. conceived and designed the experiments. Z.J.X., Y.J.L. and T.B.M. performed the experiments. Z.J.X., Y.J.L. and X.Q.L. analyzed the results. Z.J.X., Y.J.L., R.Z. and X.M.C. wrote the manuscript. All authors reviewed the manuscript.
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Electronic supplementary material
Rights and permissions
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
About this article
Cite this article
Xia, Z., Lu, Y., Li, X. et al. Upregulation of KSRP by miR-27b provides IFN-γ-induced post-transcriptional regulation of CX3CL1 in liver epithelial cells. Sci Rep 5, 17590 (2015). https://doi.org/10.1038/srep17590
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/srep17590
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.