Abstract
Tigecycline has been regarded as one of the most important last-resort antibiotics for the treatment of infections caused by extensively drug-resistant (XDR) bacteria, particularly carbapenem- and colistin-resistant Klebsiella pneumoniae (C-C-RKP). However, reports on tigecycline resistance have been growing. Overall, ~ 4000 K. pneumoniae clinical isolates were collected over a five-year period (2017–2021), in which 240 isolates of C-C-RKP were investigated. Most of these isolates (91.7%) were resistant to tigecycline. Notably, a high-risk clone of ST16 was predominantly identified, which was associated with the co-harboring of blaNDM-1 and blaOXA-232 genes. Their major mechanism of tigecycline resistance was the overexpression of efflux pump acrB gene and its regulator RamA, which was caused by mutations in RamR (M184V, Y59C, I141T, A28T, C99/C100 insertion), in RamR binding site (PI) of ramA gene (C139T), in MarR (S82G), and/or in AcrR (L154R, R13Q). Interestingly, four isolates of ST147 carried the mutated tet(A) efflux pump gene. To our knowledge, this is the first report on the prevalence and mechanisms of tigecycline resistance in C-C-RKP isolated from Thailand. The high incidence of tigecycline resistance observed among C-C-RKP in this study reflects an ongoing evolution of XDR bacteria against the last-resort antibiotics, which demands urgent action.
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Introduction
Antimicrobial resistance (AMR) is an inevitable evolutionary process conducted by organisms through genetic mutation to survive in lethal selective pressure, particularly antimicrobial agents. It has been declared by the World Health Organization that AMR is one of the top ten global public health threats confronting humanity1. According to the comprehensive statistical analysis of data globally collected in 2019, AMR was estimated to be associated with more than 4.9 million deaths2. Unless the action is taken, the burden of AMR is predicted to cause 100 trillion USD cumulative global economic cost per year by 20503. One of the most problematic AMR bacteria is carbapenem-resistant Enterobacterales (CRE), especially Klebsiella pneumoniae, which has been reported with the highest rate of carbapenem resistance among the members of the Order2. K. pneumoniae is a natural inhabitant of gastrointestinal tract of healthy humans and animals. It is a potential community-acquired pathogen and a common nosocomial pathogen causing urinary tract infection, pneumonia, meningitis, and sepsis, which account for about one-third of all gram-negative infections4. As the treatment continues, the global prevalence of antimicrobial resistance in K. pneumoniae is progressively rising5. The emergence of carbapenem-resistant K. pneumoniae (CRKP) has given the worst challenge to global public health since it has seriously restricted the available treatment options to specific antibiotics such as colistin, tigecycline, aminoglycosides, and fosfomycin6. Furthermore, because of colistin usage, colistin resistance has also emerged among CRKP, which resulted in an extremely drug-resistant phenotype. The occurrence of carbapenem- and colistin-resistant K. pneumoniae (C-C-RKP) intensified the risk posed by AMR, as colistin is one of the limited remaining treatment options. To deal with such extreme resistant phenotype, the remaining last-resort antibiotic, tigecycline, has been prescribed6. Tigecycline is a minocycline-derivative semisynthetic glycylcycline class antibiotic7. It has a bacteriostatic activity and similar mechanism of action to other tetracyclines by acting as a bacterial protein translation inhibitor interrupting an elongation of the peptide chain via reversible binding to 30s subunit of bacterial ribosome, which results in prevention of amino acid residue incorporation, lack of peptide chain formation, and consequently inhibit bacterial growth8. Moreover, the unique structure of tigecycline results in its broad spectrum of activity, as compared to other tetracyclines, and ability to combat tetracycline resistance mechanisms such as acquisition of tetracycline-specific efflux pump and protection of ribosomal protein. However, during the last decade, tigecycline resistance has been increasingly reported9. To date, several tigecycline-resistance mechanisms among K. pneumoniae have been identified. The most common one is chromosomal-mediated overproduction of nonspecific resistance-nodulation-division (RND) efflux pumps such as AcrAB-TolC10. In addition, mutations in the rpsJ gene, encoding the ribosomal protein S10, have been reported to reduce tigecycline susceptibility11. Apart from chromosomal mediated, plasmid-mediated mechanisms such as acquisition of tet(A) gene, tmexCD1-toprJ1 gene cluster, and tet(X) gene, which encodes major facilitator superfamily (MFS) efflux pumps, RND-family efflux pump, and tigecycline-modifying enzyme, respectively have also been reported12,13,14. However, the mechanism of tigecycline resistance is not fully understood and additional mechanisms may remain to be discovered.
To unveil the prevalence and mechanisms of tigecycline resistance among C-C-RKP in Thailand, clinical C-C-RKP isolates were obtained from public hospitals. Antimicrobial susceptibility profile, clonal relatedness, and the presence of acquired carbapenemase (blaKPC, blaNDM, blaOXA-48-like, blaIMP and blaVIM), colistin (mcr-1 to mcr-9), and tigecycline (tet(A), tet(X) and tmexCD1-toprJ1) resistance genes were investigated. For chromosomal-mediated tigecycline resistance, an efflux pump activity was assayed, then expression level of AcrAB efflux pump, and the regulator RamA were measured. Finally, whole genome sequencing was performed to elucidate the mutations associated with tigecycline resistance including AcrR (AcrAB-TolC efflux pump local repressor), RamR, MarR, and SoxR (global regulators repressors), OqxR (OqxAB efflux pump local repressor), Lon protease and rpsJ gene.
Results
Antimicrobial susceptibility profile
Of 240 C-C-RKP isolates, 220 isolates (91.7%) were resistant to tigecycline, and the MIC50 and MIC90 of tigecycline were 2 μg/ml and 4 μg/ml, respectively. Among tigecycline-resistant isolates, as shown in Table 1, most isolates exhibited high-level resistance to several last-resort antibiotics. The MIC50/MIC90 of imipenem, meropenem, colistin, and tigecycline were 256/256, 128/256, 64/128, and 2/4 µg/ml, respectively. Furthermore, most of the isolates were resistant to fosfomycin (70.4%), an important treatment option, with MIC50/MIC90 of 512/512 µg/ml. However, a large proportion of these isolates remained susceptible to gentamicin (30%), and chloramphenicol (29.1%).
Acquired antibiotic resistant mechanisms
Based on single-plex and multiplex PCR, tigecycline-resistant isolates were screened for acquired antibiotic resistance determinants including blaKPC, blaNDM, blaIMP, blaVIM, blaOXA-48-like, mcr-1–mcr-9, tet(A), tet(X), and tmexCD1-toprJ1. The major mechanism of carbapenem resistance was coexistence of blaNDM and blaOXA-48-like genes (55%) followed by harboring of only blaOXA-48-like gene (34.5%) and only blaNDM gene (10%), respectively. On the other hand, blaKPC, blaIMP, and blaVIM genes were not detected. For acquired tigecycline resistance, 4 isolates were found to harbor tet(A) gene while tet(X) and tmexCD1-toprJ1 genes were not detected (Fig. 1). None of the isolates were found to carry mcr genes.
Dendrogram based on ERIC-PCR typing, antimicrobial susceptibility profile, resistance gene profile, and efflux pump activity of 57 C-C-RKP isolates with tigecycline MIC ranged from 4 to 8 µg/ml. The black, gray, and white color in antimicrobial susceptibility profile indicate resistant, intermediate, and susceptible phenotype to an antibiotic, respectively. aFold reduction of tigecycline MIC in presence of 20 µg/ml CCCP compared to absence of CCCP. bScreening of tigecycline resistant gene including tet(A), tet(X), and tmexCD1-toprJ1.
Chromosomal-mediated tigecycline resistance
All tigecycline-resistant C-C-RKP isolates (MIC > 0.5 µg/ml) were tested for efflux pump activity. Twenty-nine isolates showed tigecycline MIC reduction ≥ 4-fold in the presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP). This result indicated a positive efflux pump activity (Table S2). Among these isolates, 12 isolates were from high-level tigecycline resistance group (TGC MIC 4–8 µg/ml) while 17 isolates were from low-level tigecycline resistance group (TGC MIC 1–2 µg/ml) (Table S2).
The expression level of AcrAB-TolC efflux pump and the regulator RamA among efflux pump positive isolates were evaluated using qRT-PCR. As compared to K. pneumoniae ATCC 13883, the result revealed an alteration in expression level of the efflux pump (acrB) and the regulator (ramA) (Table S2). Among the high-level tigecycline-resistant group, most isolates showed upregulation of acrB (1.2 to 8.1-fold) and upregulation of ramA (1.9 to 24.9-fold). Conversely, in the low-level tigecycline-resistant group, expression of acrB was slightly increased (1.01 to 2.63-fold) while expression level of ramA was diverse (1.50 to 9.83-fold).
The expression levels of acrB and ramA between the two tigecycline-resistant groups were evaluated using Mann–Whitney U test. The result showed that acrB expression level was significantly increased in high-level tigecycline-resistant group when compared to that of low-level tigecycline-resistant group (P = 0.0002) (Fig. 2a). However, the expression level of ramA showed no significant difference between these two groups (P = 0.3698) (Fig. 2b).
Relative expression level of (a) acrB, and (b) ramA of tigecycline high-level resistant isolates (TGC MIC > 2 µg/ml, N = 12), and tigecycline low-level resistant isolates (TGC MIC 1–2 µg/ml, N = 17). ***P = 0.0002; Mann–Whitney U test.
Whole genome sequence (WGS) analysis of tigecycline resistant isolates
To identify the causes of tigecycline resistance in C-C-RKP, 15 isolates with positive efflux pump activity were selected and subjected to whole genome sequencing. The analysis focused on mutations in the regions which have been reported to have a correlation with the overexpression of AcrAB-TolC efflux pump, including RamR (ramA repressor), PI and PII regions (recognition sites of RamR in romA-ramA operon), AcrR (acrAB repressor), and Lon protease (ramA regulator). Mutations within repressors of the other AcrAB-TolC regulators including MarA and SoxS were also considered. Apart from AcrAB-TolC efflux pump, other mechanisms reported to confer tigecycline resistance including mutations in rpsJ gene (a ribosomal S10 encoding gene), mutation in OqxR (OqxAB efflux pump regulator), acquisition of Tet(A) efflux pump, TmexCD1-ToprJ1 efflux pump, and Tet(X) (a tigecycline degrading enzyme) were investigated. WGS analysis revealed that most of the isolates contained mutations within RamR except for XDR-KP-047 and XDR-KP-059 (Table 2). Among high-level tigecycline-resistant isolates, three major patterns of RamR mutations were observed. First, a certain number of isolates were presented with a C-nucleotide insertion in ramR gene at position 99 or 100, leading to a frameshift mutation causing premature stop codon at amino acid position 53. These isolates also possessed C139T point mutation in RamR recognition site PI, which might prevent RamR binding leading to the overexpression of ramA and acrB. Second, certain isolates had S157P mutation in RamR without additional mutations in either RamR recognition site PI or AcrR. Third, a group of isolates possessed co-mutations of RamR and RamR recognition site PI, and/or AcrR. For instance, XDR-KP-113 contained double mutations in RamR (A28T and I141T) and RamR recognition site PI (C139T), XDR-KP-051 carried a single point mutation within both RamR (A20D) and AcrR (W50R), and XDR-KP-206 possessed co-mutation within these three regions.
Among low-level tigecycline-resistant isolates, most of which also possessed mutations in RamR, and RamR recognition site PI. However, most of these mutations, especially I141T and A19V in RamR and R13W in AcrR, have been reported to have no effects on overexpression of RamA. In addition, mutations within MarR and SoxR, the repressor of MarA and SoxS, respectively, were investigated. Within MarR, as compared to K. pneumoniae ATCC 13883, a single point mutation S82G was detected in all isolates and double mutations S3N/S82G were detected in 3 isolates of high-level tigecycline-resistant group. No mutations were detected for SoxR. Apart from an overexpression of AcrAB-TolC efflux pump, four isolates were found to harbor Tet(A) efflux pump. All of them were the mutated Tet(A) protein, which contained 7 mutations including I5R, V55M, I75V, T84A, S201A, F202S, V203F. Two of the four isolates, XDR-KP-011 and XDR-KP-047, also possessed overexpression of acrB (Table 2). Thus, co-existence of the mutated Tet(A) efflux pump and overexpression of acrB were believed to be the cause of high-level tigecycline resistance (TGC MIC 8 µg/ml) in these two isolates. For the other 2 isolates, XDR-KP-059 and XDR-KP-072, Tet(A) appeared to be solely responsible for tigecycline resistance since the expression of acrB was only 1.5-fold upregulated. Mutations in rpsJ and OqxR repressor were not found and mobilized tigecycline resistance genes including Tet(X) and TmexCD-ToprJ efflux pump were not detected.
Clonal relatedness among tigecycline resistant C-C-RKP
The isolates showing tigecycline MIC ranging from 4 to 8 µg/ml were selected to perform ERIC-PCR. According to the result, similarity of genotypic fingerprints among these isolates was ranging from 51.4 to 100%. Clusters were differentiated using 85% similarity, and ERIC types were classified using 95% similarity. Twenty-four clusters were distinguished (cluster A to X) and two major clusters including cluster I (14%) and W (12%) were identified (Fig. 1). Among these clusters, 51 ERIC types were differentiated. The major ERIC types were A1, I3, L1, M1, and W1. The ERIC type L1 contained three indistinguishable C-C-RKP harboring blaOXA-48-like gene. All these isolates possessed low MIC of imipenem and meropenem (16 µg/ml). However, these isolates showed high-level resistance to other antibiotics including colistin (MIC 16 µg/ml), chloramphenicol (MIC > 256 µg/ml), amikacin (MIC > 256 µg/ml), gentamicin (MIC > 128 µg/ml), and fosfomycin (MIC > 512 µg/ml). Furthermore, ERIC type L1 members also exhibited high-level tigecycline resistance (MIC 4 µg/ml) but without efflux pump activity. ERIC type I3 and W1 were found to co-harbor blaNDM and blaOXA-48-like genes. The ERIC type I3 contained two C-C-RKP isolates, XDR-KP-136 and XDR-KP-168, which were collected from different hospitals. Yet, both isolates possessed XDR phenotype with high-level resistance to several antibiotics especially imipenem (MIC > 256 and 64 µg/ml), meropenem (MIC 256 and 64 µg/ml), and colistin (MIC 64 and 32 µg/ml). These isolates also had high-level tigecycline resistance (MIC 4 µg/ml). ERIC type W1 contained two indistinguishable C-C-RKP isolates but different carbapenem-resistance characteristics. XDR-KP-069 and XDR-KP-070 co-harbored blaNDM and blaOXA-48-like genes. Both isolates displayed high-level of resistance to colistin (MIC 128 µg/ml) caused by mutations in pmrA and pmrB genes, and to fosfomycin (MIC > 512 µg/ml). These isolates also showed high-level resistance to tigecycline (MIC 4 µg/ml) with an activity of efflux pump. In addition, these isolates possessed a major frameshift mutation in ramR and a mutation in RamR recognition site PI, resulting in an overexpression of both ramA and acrB. These results suggested that the clonal dissemination of chromosomal AcrAB-TolC mediated tigecycline resistance was found among some isolates. However, the dissemination of acquired resistance genes including carbapenemase and tet(A) genes using the horizontal gene transfer were detected since same genes were found among the different clusters, especially for tet(A), which was identified from different ERIC types.
According to the MLST analysis, five STs including ST15, ST16, ST101, ST147, and ST231 were identified. The major STs among tigecycline-resistant C-C-RKP isolates were ST16 and ST147. In ST16, most of the isolates were associated with co-harboring of blaNDM and blaOXA-48-like genes. For tigecycline resistance, overexpression of acrB and ramA appeared to be the main mechanism, which was caused by the co-mutations of ramR gene, RamR recognition site (PI promoter) and/or acrR gene. In ST147, four isolates were identified and most of them harbored only blaNDM gene. Interestingly, all isolates were found to harbor the mutated tet(A) gene which encoded 7 mutations including I5R, V55M, I75V, T84A, S201A, F202S, V203F, compared to wild-type Tet(A) (GeneBank accession number X00006.1). Based on cgSNP, phylogenetic analysis of all published genomes of tigecycline-resistant K. pneumoniae isolates from more than nine countries across the world (15 from this study and 76 from NCBI database) revealed eight major clades (Fig. 3). The MIC levels and mechanisms of tigecycline resistance appeared to be diverse. Notably, the co-existence of AcrAB-TolC overexpression and Tet(A) efflux pump consistently conferred high-level of resistance. The C-C-RKP isolates in our study were classified into 4 clades, including clade 1, 2, 4, and 7. In clade 1, C-C-RKP ST101 and ST231 were closely related to K. pneumoniae ST383 clinically isolated from Lebanon15. This isolate showed pan drug-resistant (PDR) phenotype, which harbored 47 AMR determinants including carbapenemase genes (blaNDM-5 and blaOXA-48), chromosomal-mediated colistin resistance, as well as tigecycline resistance due to mutations in RamR and Tet(A) efflux pump (X61367). All members of clade 2 belonged to clonal group 15, which included OXA-232-producing C-C-RKP ST14 isolates from South Korea16, and K. pneumoniae ST15 isolates from the United States17 and China18. Two C-C-RKP ST15 from our study showed close association with clinical isolates from the USA17 and China18. The isolate from the USA showed PDR phenotype which harbored blaNDM-1 for carbapenem resistance, disrupted mgrB gene for colistin resistance, mutated ramR and mutated Tet(A) efflux pump (GeneBank accession number X61367.1) for tigecycline resistance. An additional ST15 strain from China was KPC-2-producing K. pneumoniae which was resistant to almost all tested antibiotics except for colistin. This isolate showed high-level tigecycline resistance which was caused by mutations in ramR and acquisition of tet(A). Clade 4 consisted of our ST16 isolates and K. pneumoniae ST17 isolates from China19. Lastly, clade 7 comprised all of our ST147, which showed close relationship with a NDM-5 and OXA-181 co-producing K. pneumoniae ST147 from South Korea20. In contrast to our ST147 isolates, the Korean isolate did not carry a mutated Tet(A) efflux pump. Instead, it exhibited tigecycline resistance due to the insertion of a DNA fragment flanking the RamR recognition site within the romA gene. Furthermore, our ST147 isolates shared the same origin with KPC-2 and NDM-1 co-producing K. pneumoniae ST464, which also harbored mutated Tet(A) efflux21.
Phylogenetic analysis based on core genome single nucleotide polymorphisms (cgSNP) of 15 C-C-RKP isolates from this study (red labeled) and 76 isolates of tigecycline-resistant K. pneumoniae retrieved from NCBI database.
Discussion
The emergence of carbapenem and colistin resistance in K. pneumoniae has intensified the antimicrobial resistance problem since few treatment options are left available. Tigecycline is one of the last-resort drugs that has been recommended for treatment of C-C-RKP infection5. Nevertheless, tigecycline resistance has been increasingly reported. In this study, a high rate of tigecycline resistance (91.7%) was observed among C-C-RKP, consistent with recent findings in Thailand that tigecycline resistance rate in carbapenem-resistant K. pneumoniae ranged from 46.7 to 79.6%22,23,24. However, it is worth noting that a clinical breakpoint of tigecycline resistance for Klebsiella spp. is not yet available in CLSI and EUCAST interpretive guidelines. Thus, tigecycline resistance rates in these studies were reported based on EUCAST breakpoints recommended for E. coli and C. koseri (MIC > 0.5 µg/ml)25. In recent years, it has been demonstrated that the dosage regimen of tigecycline could reach the serum concentrations of up to 2 µg/ml26 and several surveillance programs have reported tigecycline MIC90 at approximately 2 µg/ml among Klebsiella isolates27,28. Therefore, in accordance with these studies, the isolate with tigecycline MIC greater than 2 µg/ml were considered by US-FDA29 and BSAC30 as a non-susceptible and a resistant isolate, respectively. Interestingly, although 2 µg/ml was applied, tigecycline resistance rate among C-C-RKP in our study was still high (23.8%). This rate was even higher than that of the other global reports in carbapenem-resistant isolates, ranging from 1.9 to 8.9%31,32,33,34,35.
Apart from carbapenems, colistin, and tigecycline, the isolates in our study also showed high rate of resistance to several antibiotics. However, up to 70% of the isolates were still susceptible to gentamicin and chloramphenicol, coherent with the report of the National Antimicrobial Resistance Surveillance Center Thailand (NARST) in 202236 and with the other study37 that the susceptibility rates of gentamicin and chloramphenicol among K. pneumoniae were higher than 80%. Moreover, several studies has highlighted the potential of these two agents as treatment options against multidrug-resistant K. pneumoniae (MDR-KP)22,38. Therefore, gentamicin and chloramphenicol seem to be the most effective agents against MDR-KP, in vitro. Treatment of infection using these drugs in combination with β-lactams/β-lactamase inhibitor reserved for MDR bacteria such as ceftazidime-avibactam, meropenem-vaborbactam, and imipenem-relebactam may provide the best treatment outcome6.
Characterization of carbapenem-resistance mechanisms in this study revealed that co-carriage of blaNDM and blaOXA-48-like genes was the most prevalent genotype, followed by blaNDM gene, and blaOXA-48-like gene, respectively. However, other carbapenemase genes including blaIMP, blaVIM, and blaKPC were not detected. These findings are in an accord with the results from the previous studies that the prevalence of K. pneumoniae carrying blaNDM and/or blaOXA-48-like genes in Thailand is much higher than that of blaIMP, blaVIM and blaKPC genes39,40,41,42. Noteworthy, blaKPC genes in Thailand is extremely rare.
In this study, acrB overexpression was found in most tigecycline resistant isolates, most of which were associated with the upregulation of ramA. This is in accord with the previous studies. Tigecycline resistance in K. pneumoniae has been reported to associate with chromosomal-mediated overexpression of AcrAB-TolC efflux pump10,43,44,45,46,47. Expression of the efflux pump genes (acrA and acrB) has been found to significantly increase in the resistant isolates43,45,47,48. In addition, linear correlation between expression level of acrB and tigecycline MIC has been reported43,45. Generally, the expression of the efflux pump genes is controlled by their local repressor AcrR, and the global activators such as RamA, MarA, and SoxS10,49. Therefore, the upregulation of the efflux pump genes has been frequently reported with overexpression of these regulators, especially RamA12,43,50,51.
Overexpression of RamA has been reported to be caused by several mechanisms, including dysfunctional mutations in RamR, mutations in RamR binding site within RamA promoter regions, and mutations of Lon protease. ramR is a transcriptional repressor gene belonging to the TetR family. It encoded the RamR repressor, which binds to the promoter regions of the ramA gene, resulting in transcriptional repression. RamR protein consists of 194 amino acids forming 9 alpha helices protein which separated into N-terminal DNA binding domain (α1 to α3), and C-terminal dimerization domain (α4 to α9)52. The loss-of-function mutations in RamR have been reported from several species especially K. pneumoniae, which resulted in ramA overexpression12,53,54. In addition, computational analyses have indicated that mutations in the dimerization domain of RamR can lead to a reduction in its DNA binding affinity in Salmonella spp.55. In our study, mutations in RamR were detected in both DNA binding and dimerization domains but mostly were point mutations that occurred outside hotspot regions. A frameshift mutation from a single nucleotide insertion was detected in two isolates. Most of the isolates with RamR mutations showed overexpression of RamA. In addition, mutations that have been associated with an inability to induce RamA upregulation, including A19V and I141T were detected12,43,44,51,53. Apart from RamR, in this study, the mutation within RamR recognition site was also identified. RamR binds to two regions upfront of ramA gene (PI and PII promoters) for regulating activity51. Yet only a mutation within the PI promoter region was detected. Notably, a C139T point mutation was identified in most of our isolates, which suggests the presence of a potential hotspot within the PI promoter region. Mutations in Lon protease that has been reported to associate with RamA upregulation were not detected56. Nevertheless, there were some isolates that showed no correlation between these two genes; upregulation of ramA was found without overexpression of acrB. To explain this discrepancy, further investigations are required. Noteworthy, one isolate with high-level tigecycline resistance, XDR-KP-185 (MIC 4 µg/ml), showed only 1.28-fold of acrB expression level while known-acquired resistant determinants were not detected. This might indicate an activity of other mechanisms, such as OqxAB or KpgABC efflux pump, which have also been reported to confer tigecycline resistance50,57,58. Since tigecycline-resistance mechanisms are complex and may involve several efflux pumps and regulators, enhancing the sensitivity of an efflux pump inhibition assay may require the use of multiple types of efflux pump inhibitors. In addition, it may be beneficial to measure the expression levels of other efflux pumps, such as OqxAB, and the expression levels of other global regulators such as MarA, SoxS, and RarA.
This study identified several important STs among XDR- K. pneumoniae isolates, including ST15, ST16, ST101, ST147, and ST231. Also, this was the first characterization of tigecycline-resistance mechanism from these STs in Thailand. In this work, ST16 was identified as the predominant clone. ST16 is globally known for its high-risk profile associated with both hypervirulent and drug-resistant characteristics. To date, the emergence of K. pneumoniae ST16 has been reported from several countries, including Denmark59,60, France61, Italy62, United Kingdom63, Ireland63, Croatia64, Bulgaria65, Canada66, Thailand67,68, Vietnam69, China70, Egypt71, and Brazil72, which covered 4 of 7 continents. Interestingly, very few studies demonstrated the association between tigecycline resistance and this high-risk clone. Nevertheless, K. pneumoniae ST16 has been reported to accumulate various types of antimicrobial resistance determinants73 such as blaCTX-M-1561, blaKPC74, blaNDM-560, blaOXA-48-like75, blaNDM-1 and blaOXA-23262,67,68, aac(6′)-lb-cr64, aac(6′)-lb67, qnrS67, and mgrB mutation67,69. In our study, the accumulation of many antimicrobial resistance determinants (blaCTX-M-15, blaNDM-1, blaOXA-232, aadA1, aadA2, aac(6′)-lb-cr, and aac(6′)-lb) was also detected in C-C-RKP ST16 isolates. To the best of our knowledge, this study was the first to demonstrate the connection between the clonal expansion of K. pneumoniae ST16 and a high rate of tigecycline resistance among C-C-RKP isolates. Therefore, our finding could provide potential evidence to support the ongoing evolution of the K. pneumoniae ST16 against the last-resort antibiotics, indicating the capability of this high-risk clone to pose a significant public health threat. Another significant ST was ST147, recognized as a successful high-risk clone within K. pneumoniae which was reported to be associated with β-lactams and fluoroquinolones resistance76. ST147 has been reported globally, with multiple outbreaks associated with this ST, except in Antarctica4. It has been found to harbor various antimicrobial resistance genes, such as extended-spectrum β-lactamase, carbapenemase, aminoglycosides resistance, fluoroquinolone resistance, and tet(A) gene. In Thailand, ST147 carrying blaNDM have been reported in several studies24,77. However, the major clonal group of K. pneumoniae-CG258, comprising ST11, ST258, and ST512, was not detected in our study4.
The only acquired tigecycline-resistance mechanism detected in our study was the Tet(A) efflux pump. Four isolates of ST147 were found to harbor the same mutated Tet(A), which contained 7 mutations (I5R, V55M, I75V, T84A, S201A, F202S, V203F). This type of mutated Tet(A) was similar to that of the previous reports from China and Taiwan12,21,78. The mutated Tet(A) has been proved to confer tigecycline resistance via gene cloning experiment, which elevated tigecycline MIC of the susceptible organism up to 8-fold12,78. The gene has been reported to be located on plasmids21,78, suggesting a possibility of conjugative transfer of this gene to different bacterial species. Moreover, the co-existence of this mutated efflux pump with dysfunctional RamR has been reported to exacerbate tigecycline-resistance level12. In our study, the mutated Tet(A) was found to co-exist with acrB overexpression, which was found to confer high-level tigecycline resistance (MIC 8 µg/ml) in two isolates. This result suggests that beside dysfunctional RamR, the co-occurrence of the mutated Tet(A) with other resistance mechanisms may also be able to confer high-level tigecycline resistance. To our knowledge, this is the first report of mutated Tet(A) harboring C-C-RKP in Thailand. Therefore, presence of the mutated Tet(A) should be carefully monitored. Interestingly, we found that an efflux pump inhibitor CCCP could re-sensitize the isolates to tigecycline that harbor only Tet(A) resistance mechanism. The presence of 20 µg/ml CCCP could reduce the MIC of tigecycline from 2 and 4 µg/ml to 0.125 µg/ml in XDR-KP-059 and XDR-KP-072, respectively. This re-sensitization of isolates to tigecycline indicates effectiveness of an efflux pump inhibitor which could be a promising choice to combat Tet(A) harboring K. pneumoniae.
Methods
Bacterial strains
A total of 4179 K. pneumoniae clinical isolates were collected from public hospitals including King Chulalongkorn Memorial Hospital, Chulalongkorn University, and the Golden Jubilee Medical Center, Mahidol University, during 2017 to 2021. Of these isolates, 240 C-C-RKP isolates were obtained for further investigation. Bacterial species was identified by biochemical testing and MALDI Biotyper system (Bruker Daltonik, Leipzig, Germany).
Antimicrobial susceptibility testing
The minimum inhibitory concentration (MIC) of 9 antibiotics including amikacin, chloramphenicol, imipenem, fosfomycin, gentamicin, meropenem, tetracycline, and tigecycline was investigated by agar dilution while that of colistin was measured by broth microdilution, then the results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI, 2023) guidelines79. Specifically for tigecycline, resistance cutoff at MIC > 0.5 µg/ml for E. coli and C. koseri was used according to European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2023) document25. E. coli ATCC 25922 and P. aeruginosa ATCC 27853 were used as reference strains.
Clonal analysis
Genomic DNA (gDNA) of tigecycline-resistant C-C-RKP was extracted using TIANamp Genomic DNA KIT (TIANGEN, China) according to manufacturer’s instructions. ERIC-PCR was modified according to the protocol published by James and colleagues80. The thermal cycling conditions included initial denaturation at 94 °C for 5 min, 35 cycles of denaturation at 94 °C for 1 min, annealing at 38 °C for 1 min, extension at 72 °C for 3 min, and followed by final extension at 72 °C for 10 min. The PCR products were loaded onto a 1.5% agarose gel and ran at 100 V for 1 h, then the result was visualized using gel-docking system. The ERIC pattern was analyzed using InfoQuest FP software version 4.5 by Dice coefficient, unweighted pair group method with arithmetic means (UPGMA) at 1% optimization and 1% band position tolerance. A cluster of the isolates was differentiated at 85% similarity, and an ERIC type was differentiated at 95% similarity. Multilocus sequence typing (MLST) was performed based on 7 housekeeping genes for K. pneumoniae including gapA, infB, mdh, pgi, phoE, rpoB, and tonB. The allelic number and sequence type (ST) were determined by comparing with MLST database using online PubMLST database81.
Detection of acquired antimicrobial resistance genes
Carbapenemase genes (blaKPC, blaNDM, blaIMP, blaVIM, and blaOXA-48-like)82 and mobilized colistin resistance genes (mcr-1 to mcr-9) were detected by multiplex PCR according to our previous studies83,84. Acquired tigecycline resistance genes including tet(X)85, tet(A)86, and tmexCD1-toprJ187 were screened by single-plex PCR. The final 20 µl reaction contained 0.5 µM of each primer, 1.5 mM of MgCl2, 200 µM of dNTPs, and 2.5 units of DNA polymerase. The PCR conditions were slightly modified from the original studies. Briefly, the conditions included the initial denaturation at 96 °C for 3 min, 30 cycles of denaturation at 96 °C for 30 s, annealing at 52 °C for tet(X), 55 °C for tmexC1, and 59 °C for tet(A) for 30 s, extension at 72 °C for 30 s, and final extension at 72 °C for 10 min. The primers used for single-plex and multiplex PCR were listed in Table S1.
Efflux pump inhibition assay
Efflux pump activity was assayed using efflux pump inhibitor, carbonyl cyanide m-chlorophenylhydrazone (CCCP) (TCI, Japan). Briefly, MIC of tigecycline was evaluated using agar dilution method in the presence and absence of 20 µg/ml of CCCP. Then, a 4-fold or greater reduction of tigecycline MIC observed in the presence of CCCP, compared to the absence of CCCP, was considered as positive for efflux pump activity88.
Measurement of AcrAB efflux pump expression level
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to assess the expression levels of efflux pump gene acrB, and transcription activator gene ramA. The expression level of rrsE housekeeping gene was also measured and served as the reference for normalizing the expression level of each target gene. The specific primers were listed in Table S143. Total bacterial RNA was extracted from log-phase culture using TRNzol Universal Reagent (Tiangen, China), and was treated with DNase I (ThermoFisher Scientific, Waltham, USA). Then, qRT- PCR was performed with KAPA SYBR® FAST One-Step qRT-PCR (KAPA BIOSYSTEMS (PTY) LTD, South Africa). The final 15 µl PCR reaction included 30 ng of DNase treated RNA, 0.4 µM of primers for acrB reaction and 0.15 µM of primers for ramA and rrsE reactions. The PCR condition included cDNA synthesis at 42 °C for 15 min, pre-denaturation at 95 °C for 5 min, and 40 cycles of denaturation at 95 °C for 15 s and annealing at 60 °C for 60 s. The result was analyzed using CFX Maestro software based on the 2−ΔΔct method43. Relative expression level of the mRNA was compared to that of K. pneumoniae ATCC 13883, a tigecycline-susceptible reference strain.
Whole genome sequencing
Single colony of the selected C-C-RKP isolates with tigecycline-resistant phenotype was cultured overnight. Then, gDNA was extracted and purified using DNeasy® Blood and Tissue Kit (QIAGEN, Germany). The library preparation was performed using VAHTS Univeral DNA Library Prep kit (Vazyme Biotech, China). The gDNA was sequenced using Illumina Novaseq 6000, PE150 platform (Illumina, San Diego, CA, USA). The quality of raw read was investigated using FastQC tool (Version 0.12.1)89. Then, raw data was trimmed using TrimGalore (version 0.6.10)90. Bacterial genomes were later assembled using Spades (version 1.1.0) and Quast tool (Version 5.2.0) was performed to assess quality of the assembly91,92. Prokka (version 1.14.6) was used to annotate genes93. The antimicrobial resistance and plasmid were determined by Staramr tool (version 0.9.1) which cooperated with ResFinder, and PlasmidFinder, respectively94. The genomes were compared using the Basic Local Alignment Search Tool (BLAST, NCBI)95. The analysis of gene mutation was based on a comparison of the C-C-RKP′ genes to the reference sequence K. pneumoniae subsp. pneumoniae MGH 78578 (GeneBank accession number CP000647), and to wild-type K. pneumoniae ATCC 13883 (GeneBank accession number NZ_JSZI00000000).
Phylogenetic analysis
Core genome single nucleotide polymorphisms (cgSNP) was performed to determine the number of core genome SNP from draft genomes of tigecycline-resistant C-C-RKP clinical isolates in Thailand and from varying tigecycline-resistant K. pneumoniae WGS investigations conducted elsewhere (available on NCBI database). K. pneumoniae subsp. pneumoniae MGH 78578 (NCBI RefSeq assembly GCF_000016305.1) was used as a reference to generate a core genome alignment and phylogenetic tree was constructed using a core SNP alignment. Draft genomes of K. pneumoniae were aligned following the detection and filtration of recombinant regions using Parsnp v1.296 and Gubbins v2.4.197. Maximum-likelihood (ML) trees were generated by RAxML v8.2.1298 using ASC_GTRGAMMA model of rate heterogeneity with the Lewis correction for ascertainment bias99,100. Branch support was performed by 1000 bootstrap replicates. Best-scoring ML tree was visualized and annotated as a phylogenetic tree using FigTree v1.4.4 and Evolview v2101,102.
Statistical analysis
Statistical analysis of an association between tigecycline-resistance level and expression level of acrB and ramA was performed using GraphPad Prism 8 software. Since the data were not normally distributed, an intergroup comparison was performed using the Mann–Whitney U test. A P value < 0.05 was considered as statistical significance. The correlation between expression level of acrB and tigecycline MIC was evaluated using linear regression.
Data availability
The genomic data of 15 C-C-RKP isolates are available in the NCBI database under BioProject No. PRJNA1000742.
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Acknowledgements
We gratefully acknowledge the assistance of all staffs from clinical microbiology laboratory at King Chulalongkorn Memorial Hospital, Chulalongkorn University, and at the Golden Jubilee Medical Center, Mahidol University.
Funding
This work was supported by the Research Career Development Grant (Grant No. RSA6280021), which was co-funded by the Thailand Research Fund (TRF) and the National Research Council of Thailand (NRCT). This work was also supported by the grant for research and innovation from the NRCT (Grant No. N35A660013) and a grant from Mahidol University (Fundamental Fund: fiscal year 2023 by National Science Research and Innovation Fund (NSRF)). This work was partly supported by a graduate scholarship from the Master of Science Program in Medical Technology (International Program) at the Faculty of Medical Technology, Mahidol University.
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N.C.: methodology, investigation and analysis, data curation, preliminary draft of the manuscript and contributed equally to this work as first author. S.L.: conceptualization, funding acquisition, methodology, supervision, investigation and analysis, data curation, preliminary draft of the manuscript, editing the manuscript and contributed equally to this work as first author. T.P.: bacterial collection, investigation and analysis, data curation and editing the manuscript. S.W.: investigation and analysis and editing the manuscript. T.C.: bacterial collection, data curation, supervision and editing the manuscript. D.L.W.: bacterial collection, data curation, supervision and editing the manuscript. S.Y.: conceptualization, funding acquisition, methodology, supervision, project administration, editing and finalizing the manuscript.
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Chirabhundhu, N., Luk-In, S., Phuadraksa, T. et al. Occurrence and mechanisms of tigecycline resistance in carbapenem- and colistin-resistant Klebsiella pneumoniae in Thailand. Sci Rep 14, 5215 (2024). https://doi.org/10.1038/s41598-024-55705-2
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DOI: https://doi.org/10.1038/s41598-024-55705-2
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