Abstract
Nuclear magnetic resonance has experienced great advances in developing and translating hyperpolarization methods into procedures for fundamental and clinical studies. Here, we propose the use of a wide-bore NMR for large-scale (volume- and concentration-wise) production of hyperpolarized media using parahydrogen-induced polarization. We discuss the benefits of radio frequency-induced parahydrogen spin order transfer, we show that 100% polarization is theoretically expected for homogeneous B0 and B1 magnetic fields for a three-spin system. Moreover, we estimated that the efficiency of spin order transfer is not significantly reduced when the B1 inhomogeneity is below ± 5%; recommendations for the sample size and RF coils are also given. With the latest breakthrough in the high-yield synthesis of 1-13C-vinyl pyruvate and its deuterated isotopologues, the high-field PHIP-SAH will gain increased attention. Some remaining challenges will be addressed shortly.
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Introduction
During the last decades, a number of methods to boost the magnetic resonance (MR) signal by increasing the nuclear spin polarization were developed1,2,3,4,5. Particularly, metabolic images (MRI) with hyperpolarized pyruvate showed great potential for early diagnostics and paved the way for the extensive development of dynamic nuclear polarization (DNP)6,7,8. Parahydrogen-induced polarization (PHIP)9,10 is an alternative method with intriguing properties, including fast and continuous11,12,13, or quasi-continuous14, production of hyperpolarized molecules. Although the biomedical application of PHIP is less developed than DNP4 or spin-exchange optical pumping (SEOP)3, several metabolic studies were published recently15,16,17,18 in addition to the very first 13C MRI with hyperpolarized contrast agents19.
As a spin-0 particle, parahydrogen (pH2) does not yield a magnetic resonance signal. Thus, conversion of the para-spin order to an observable spin state is key. Interestingly, there is still no consensus upon which method yields the highest efficiency of spin order transfer (SOT) given any relevant molecule.
Hyperpolarized pyruvate is the hyperpolarized tracer that is most frequently used in vivo. The diagnostic power of hyperpolarized pyruvate has already been demonstrated in humans18, animals and in cell cultures20,21. Arguably one of the most promising approaches for generating hyperpolarized pyruvate with pH2 is PHIP-SAH (PHIP using sidearm hydrogenation). Here, homogeneous catalysis22 was reported to provide higher polarization than heterogeneous approaches23. Heterogeneous catalysis, on the other hand, may be beneficial when it comes to cleaning the sample, e.g., prior a biomedical application. An alternative to polarizing pyruvate by hydrogenation is signal amplification by reversible exchange (SABRE), where pH2 and the target molecule undergo a reversible exchange with an Ir-complex24. Here, recent breakthroughs25,26,27 enabled the 13C polarization of pyruvate to 13%, (in methanol)28. Although some conclusions drawn in this work are relevant for SABRE, too, we are not discussing this method in detail because it has a significantly different chemical and physical nature.
The transfer of spin order from pH2 to hyperpolarization of pyruvate in PHIP-SAH is a three-stage process: hydrogenation, spin order transfer, and cleavage. The starting compound is an unsaturated pyruvate ester such as vinyl, propargyl, allyl, or another unsaturated ester, which is commonly labeled with 13C in 1-position29. After hydrogenation with pH2 and SOT, the reduced ester is cleaved yielding pyruvate that is hyperpolarized at the 1-13C nucleus with a relaxation time around 1 min21,30. Hence, all manipulations have to be performed rapidly to avoid loss of polarization.
Here, we analyze different SOT strategies for PHIP-SAH. We identified RF-based SOT as the most promising method and we discuss how this approach may be implemented using a wide-bore high-field MRI system and which conditions must be met for in vivo metabolic imaging. Finally, we describe the first implementation of an approach, which provides a highly concentrated and moderately polarized 1-13C-pyruvate in an aqueous solution.
Methods
The typical sample was 100 mM vinyl pyruvate (VP) with 5 mM [Rh] = [1, 4-Bis(diphenylphosphino)butane] (1,5-cyclooctadiene)rhodium(I) tetrafluoroborate (CAS = 79,255–71-3, Merck) in chloroform-d. 1-13C-VP, 1-13C-VP-d3 and 1-13C-VP-d6 were synthesized according to Ref.31,32. 1 mL of the sample was loaded into a 10 mm heavy-wall high-pressure NMR tube (513-7PVH-7, Wilmad-LabGlass). Before hydrogenation, the sample was preheated to 55 °C. When placed in the NMR spectrometer, the sample was flushed with pH2 at 10 bar for 5–22 s. Then ESOTHERIC SOT33 was applied. A 9.4 T high-resolution wide-bore NMR (Avance NEO, Bruker) was used here for polarization and imaging. A 1 T benchtop NMR (Spinsolve Carbon, Magritek) was used for external observation of hyperpolarization. More details in supporting materials (SI) together with additional experimental results.
All presented simulations were done using the MOIN spin library34 and scripts are available in SI.
Results and discussion
Theoretical analysis
An ideal type of PHIP-SAH precursor
Allyl pyruvate, propargyl pyruvate, and vinyl pyruvate (VP) are the main precursors for the hydrogenation and subsequent SOT to the 13C-nuclei of pyruvate29. VP has the strongest coupling between the added pH2 and the target 13C, and so and so it requires the least amount of time for SOT, reducing the relaxation losses. Hence, VP appears to be the precursor of choice. However, it is difficult to synthesize and the reported chemical yields did not exceed 10%29,35.
Recently, we found a more efficient synthesis (yield > 60%) of 1-13C-VP allowing us to also synthesize fully deuterated 1-13C-VP-d631,32. The 1-13C-VP-d6 molecule looks like an ideal precursor for PHIP-SAH because all interfering J-couplings are prevented by deuteration. With such high yields, the synthesis can be scaled up to industrial production. 1-13C-VP-d6 can also be prepared at relatively low costs (several thousand euros per gram). However, the preparation of medical-grade 1-13C-VP-d6 will likely be significantly more challenging and expensive. 1-13C-ethyl pyruvate-d6 (1-13C-EP-d6) is the product of the hydrogenation of 1-13C-VP-d6. After SOT and subsequent cleavage of the sidearm22, 1-13C-pyruvate-d3 is produced (Fig. 1).
Limitations of current state magnetic field cycling (MFC)
For PHIP-SAH, there are two main approaches to transfer the spin order from pH2 to the nucleus of choice: ultra-low magnetic field cycling (MFC-SOT)36,37 and radiofrequency induced spin order transfer (RF-SOT)38,39,40.
MFC-SOT theoretically enables to polarize the chosen heteronuclei to 100% (usually 13C or 15 N) in a system of three spins (Fig. 2)41,42. The concept of level anti-crossings (LAC)43 was instrumental in understanding the spin-order transfer during MFC. The spin-order populations follow the adiabatic energy levels, and, if an appropriate LAC is passed, the product can be polarized41,44. A lower efficiency of spin-order transfer was predicted in systems with more than 3 spins37. In a proof of principle study, Reineri et al. investigated the efficiency of MFC to transfer the parahydrogen spin order to 13C across three and four bonds37 using ethyl 1-13C-propionate; which is a good model for ethyl 1-13C pyruvate. Both molecules can be reduced to a six-spin system of A3B2X type with the X-spin being 1-13C, and the A3 and B2 are methyl and methylene groups, respectively. No more than 35% polarization of the X-spin was predicted after hydrogenation with pH2 in the A and B positions and subsequent MFC37. It is important to note that the low efficiency is not a problem with the particular MFC or molecule. Instead, it is a fundamental property that is bound to the symmetry of the spin system45,46. Experimentally, no more than 5% 13C polarization was achieved with MFC for 40 mM pyruvate in an aqueous solution35.
The deuteration of the PHIP-SAH precursor is also not a panacea; we illustrate this here using a four-spin system and the same adiabatic magnetic field cycling (Fig. 2). In general, LACs and SOTs happen when the difference between Larmor precession frequencies, \(\nu\), of two spins (A and X) is comparable with their interaction, \(J\): \(\left|{\nu }_{\mathrm{A}}-{v}_{\mathrm{X}}\right|\cong \left|{J}_{\mathrm{A}}^{\mathrm{X}}\right|\). The magnetogyric ratio of deuterium is 6.5 times smaller than that of a proton, which means that all scalar spin–spin 2H-13C interactions are 6.5 times smaller than the corresponding 1H-13C interactions. At the same time, the difference between the Larmor precession frequencies of 2H and 13C is about 8 times smaller than between 1H and 13C. As a result, the LACs between 1H-13C and 2H-13C will happen in approximately the same range of magnetic fields and so it will be a challenge (if even possible) to theoretically achieve 100% spin order transfer to the nuclei of choice using MFC. However, MFC optimization is an ongoing process and one can apply recently proposed decoupling of deuterium at low fields47, parametric optimization of analytical field profiles48, or algorithms of optimal control49 may improve the matter.
RF-SOT at higher magnetic fields appears to be better way to transfer polarization in certain cases because:
-
Heteronuclei are weakly coupled with hydrogens, so there is no efficient spontaneous polarization transfer to 2H, 13C and other heteronuclei;
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The effective spin system can be reduced to two protons and one (third) nucleus of choice, e.g., 13C using selective deuteration31,32 or selective excitation50,51. Note that because of the symmetry constraints45,46, a 100% polarization using only selective excitations in systems like ethyl pyruvate (A3B2X) is also not possible.
RF-induced spin order transfer at high fields
An SOT should be robust and provide 100% of 13C polarization, at least theoretically. The ESOTHERIC SOT (Fig. 3)33 looks like the most appropriate sequence for weakly coupled pH2 protons because it offers 100% 13C polarization for 1-13C-EP-d6 and does not need any frequency-selective excitation; only hard pulses are used50. It must be noted, that frequency-selective excitations are interesting alternatives, which increase the scope of the system, where 100% polarization can be expected. However, frequency-selective sequences are only usable when the magnetic field is homogeneous: here, for the sake of generality, we will assume that it is not the case. For example, in SAMBADENA experiments52, the PHIP reactor is not placed in the isocenter of the MRI magnet, which compromises the magnetic field homogeneity.
Two modifications of ESOTHERIC were proposed to improve SOT performance in an inhomogeneous B0 field: ESOTHERIC-Grad with bipolar pulsed-field gradients (BPFG) and ESOTHERIC-Ref with multiple spin-refocusing blocks (Fig. 3a)53. As it was pointed out before53, BPFG of ESOTHERIC-Grad requires advanced hardware, unlike ESOTHERIC-Ref. As both provide a comparable efficiency, we suggest using the second less demanding hardware variant. For ESOTHERIC-Ref one should apply constant gradients to worsen the magnetic field homogeneity such that radiation damping is reduced, preventing the RASER effect at high fields53,54. The amplitude of the gradients can be different during the pH2 bubbling and SOT. ESOTHERIC is robust concerning the evolution intervals (Fig. 3b). Even is the intervals are off by 20%, about 95% polarization is predicted for 1-13C-EP-d6. However, the B1 field should be relatively homogeneous and strong for efficient RF-SOT (Fig. 3c,d,e). Note, however, that we used perfect RF pulses (instantaneous rotation) and did not consider molecular motion in our simulations, hence the amplitude of the field gradients and the B0 field inhomogeneity have no impact on the RF-SOT performance.
Commonly, only one refocusing element is used40,55: for a perfectly homogeneous B0 field, the amplitude of the excitation pulses can be 5–10% off from the nominal values while still providing 95% of polarization for 1-13C-EP-d6 (Fig. 3 and Figs. S1–S3, SI). However, if the B0 field is inhomogeneous, the gradients are switched on during SOT like in ESOTHERIC-Ref, or in the presence of diffusion and/or convection, more frequent refocusing is beneficial53,56. In this case, the robustness of the SOT with a simple 180° refocusing pulse is reduced (Fig. 3e) and, it is necessary to use composite refocusing pulses (Fig. 3d). Note that already a 10% deviation of RF-pulse from the nominal value of the flipping angle with five 180° refocusing pulses changes the sign of the polarization (Fig. 3e). We illustrated the effect of RF-field imperfection on ESOTHERIC performance in greater detail and for other VP isotopomers in the Supporting Material (Figs. S1–S3, SI).
We measured the distribution of the B1 field along the Z direction for two NMR probes of the 9.4 T WB NMR spectrometer: 5 mm BBFO (Bruker, Fig. S7) and 1H/13C 25 mm mouse head resonator (Bruker, Figs. S8–S11). The method and resulting distribution of the B1 field are given in the SI. The conclusion we draw is that it is necessary to reduce the sample size significantly below the size of the coil to achieve a sufficient B1 homogeneity for close to maximum efficiency of SOT. We found that BBFO probes with a 5 mm high-pressure tube (524-PV-7, Wilmad) filled with 200 µL or 1H/13C 25 mm mouse head resonator with a 10 mm high-pressure tube (513-7PVH-7, Wilmad) filled to 1 mL should be used to meet the B1 homogeneity requirements (Tables S1–S2, SI).
Hence, the right choice of the SOT could also help to reduce radiation damping and RASER while providing close to 100% polarization, even not far from experimental conditions. For a more realistic assessment of the efficiency of the method, one should consider B1 homogeneity, finite duration of RF pulse and RF frequency offset, diffusion, and convection in an inhomogeneous B0 field including different orientations of gradients; the complex simulation of all these effects is in progress56.
The polarization hardware
PHIP-SAH, like SAMBADENA and other variants, required synchronizing the fluid handling, hydrogenation, decoupling, and the RF-SOT. This was addressed using dedicated RF-SOT polarizers57,58 or using the electronics of available cryogenic NMR, MRI, or benchtop NMR33,59. The high-field hydrogenation in a homogeneous B0 field and high concentration of the reagents induce radiation damping and RASER effects53,54,60 which both deteriorate the efficiency of SOT. Because a high concentration and polarization are desirable for an in vivo application, the radiation damping must be decreased by reducing the Q-factor, filling factor, and magnetic field homogeneity61. ESOTHERIC-Ref reduces radiation damping by switching on gradients. Hence, the wide-bore MR systems are of primary interest because they can fit larger sample volumes with a relatively low filling factor. This reduces radiation damping while keeping a high Q-factor, hence short RF pulses. Compared to mT-polarizers11,62, high-field NMR has optimized hardware for SOT and in situ analysis of the polarized media.
Traditionally, high-resolution NMR experiments are done using 5 mm tubes. For fast and efficient hydrogenation at elevated pressures, it is advisable to use high-pressure NMR tubes (e.g., 524-PV-7, 522-PV-7 Wilmad LabGlas, or analogous from Norell). Due to the small inner volume and a sensitive area of the RF coil being about 2 cm in height, the polarizable volume is below < 250 μL. This amount is too little for clinical translation. The restriction to the RF coil’s sensitive area is essential, as the SOT takes place only where proper pulses are applied. Furthermore, RF-SOT does not require extremely high B0 homogeneity. Hence, NMR probes must be adapted for larger samples even if it comes at the cost of B0 homogeneity and sensitivity to ensure a high B1 homogeneity for a given sample volume. Therefore, one should consider larger probes with larger sample volumes for the optimized polarizer. Hydrogenation in the vertical bore is also beneficial because the high column is easier to saturate with pH2, in addition, one can use commercial high-pressure NMR tubes (e.g., 513-7PVH-7, Wilmad LabGlass). As mentioned above, for the 1 mL sample size with a high-pressure 10 mm 513-7PVH-7 tube, we do not expect a significant reduction in SOT performance. Note that calculations were done for a perfectly homogeneous B0 magnetic field, an assumptions that is obviously not correct (Fig. S8, SI).
Experimental realization
It seems that for an efficient translation of PHIP-SAH to clinical applications one needs to provide polarized media not only at large volumes (> 0.5 mL) but also at large concentrations (> 50 mM), and high polarization (> 10%), while maintaining a high purity. We set out to realize the approach using the equipment at hand. First, we analyzed and optimized the hydrogenation and SOT. Next, we implemented a chloroform/water phase separation and transferred the sample for quantification using a second spectrometer.
In situ polarization
Because there are no dedicated commercial systems for large-scale PHIP-SAH at high field, we used a wide-bore MRI which was available to us with a 9.4 T magnetic field (Fig. 4). One can use imaging probes instead of traditional 5 mm probes. We used a heavy-wall NMR tube with a 10 mm diameter (513–7 PVH-7, Wilmad LabGlass) together with the 25 mm mouse head 1H/13C resonator (Bruker). Filled to 1 mL, the whole sample is completely inside the sensitive area of the coil. The 1H-MRI can be used to find the right place for the sample (Fig. 4a and S8), however, this feature is not necessary for the stand-alone polarizer.
Then the reactor (here, a 10 mm high-pressure NMR tube) was heated to 55 °C and placed into MRI, we started optimizing the hydrogenation. 100 mM of 1-13C-VP, 1-13C-VP-d3 and 1-13C-VP-d6 were hydrogenated to 1-13C-EP, 1-13C-EP-d3 and 1-13C-EP-d6, respectively in 1 mL chloroform-d. The experimentally achieved polarization was 1% for 1-13C-EP, 5% for 1-13C-EP-d3, and 6.5% for 1-13C-EP-d6 when hydrogenation time was 22 s. With such a long time at 10 bar pH2, we ensured complete hydrogenation of VP (Fig. S4, SI). However, when the hydrogenation time was reduced to 7 s, the 13C-signal achieved for 100 mM 1-13C-EP-d6 was significantly increased to 17.4% 13C polarization (Fig. S5, SI). Hence, the maximum achieved 13C-polarization for the 100 mM concentration of 1-13C-EP-d6 is equal to 1.74 M% molar polarization (concentration times polarization) and 1.74 mmol% molecular polarization (number of molecules in mole units times polarization). Both parameters are important and coupled with the total volume of the sample. These values are below the numbers achieved for acetate53, likely because of the relatively long hydrogenation time caused by semi-efficient pH2 saturation and low temperature. The hydrogenation can be accelerated further by using more efficient catalysts, by increasing the reaction temperature or the reaction surface (or volume, e.g., by delivering pH2 through porous material, such as sintered glass63, hollow fiber membranes64,65, semipermeable tubing66, or by spraying reagents into pH267). While the temperature control unit is common for standard NMR probes and was commonly used for PHIP-SAH, too33, it is not available for the imaging probe used here. Therefore, we preheated the sample in the NMR tube in a 55 °C water bath for 30 s right before insertion into MRI and pH2 hydrogenation (Methods, SI).
The observed relaxation of 13C hyperpolarization was 55 s in chloroform-d at 9.4 T. We observed that if the same tube was used for a long time without cleaning with strong acids, a black film is deposited on the surface, and the T1-relaxation time decreases down to 20–30 s. A similar effect was observed for hyperpolarized 1H signals, whose lifetime decreased down to 8–10 s.
Still, 17 % 13C polarization of 100 mM EP is quite significant and well sufficient for chemical shift imaging (CSI, Fig. 4c). The slice FID CSI parameters were: 20 × 20 mm field of view, 5° flipping angle, 2 mm slice thickness, 39 ms repetition time, 40 s total scan time, and 32 × 32 matrix size.
Quantification of polarization in and ex situ
After RF-SOT, we used a 5° excitation pulse to check the level of polarization (Fig. 4b) in situ (to determine the polarization level, one should measure a 13C-spectrum of the reagent before hydrogenation). To extract the solution, after RF-SOT, we turned the 3-way valve (see scheme Fig. 4e) and the polarized media of 1-13C-EP-d3 was flashed into the vial outside of the polarizer, driven by the system’s hydrogenation pressure. Here, we used a 0.4 M aqueous solution of NaOH to cleave the sidearm. Then we collected the top aqueous phase and filtered it through Tenax filter and, afterward, a particle filter. The resulting media was placed in the 5 mm NMR tube and measured at 1 T using a 1H/13C benchtop NMR spectrometer. The quantified polarization was 1.6% 50 s after the SOT application. With T1 being of the same order, it is consistent with the in situ acquired values of 5%: 1.6% \(\times\) e = 4.34%. After a similar purification procedure, the polarized pyruvate was used for in vivo imaging18; here we did not analyze the purity of the resulting hyperpolarized aqueous solution further. This stage of purification and delivery will benefit from automation and further optimization.
Interestingly, we observed that the 13C-T1 of hyperpolarized 1-13C-pyruvate is strongly pH-dependent. We observed 40 s at neutral and acidic conditions (pH below 7) while 60 s at basic pH (pH above 10). Using the same 1 T spectrometer and 1-13C-pyruvate polarized with dDNP we observed a 13C-T1 of 90 s30, meaning that there can be some impurities in our reagents which increases the relaxation rate of 1-13C-pyruvate.
Experimental benchmarking
To compete with dDNP, PHIP should offer a similar polarization, concentration, and sample size. The rate of production of PHIP-SAH polarized samples is significantly higher and semi-continuous hyperpolarization is possible already, i.e., we can repeat experiments every few minutes without automation14.
For dDNP, typically, 60 mM pyruvate in a 4 mL aqueous solution were polarized to 50% 13C polarization right after dissolution30. This corresponds to 3 M% molar polarization and 12 mmol% molecular polarization. Although for in vivo murine experiments no more than 200–500 μL are needed for the injection, the residual sample is often used for quality control (pH measurements, polarization quantification, and concentration quantification). 60 and 24% 13C polarization for 13 and 55 mM concentrations of 0.1 mL pyruvate precursors solution were reported recently by Glöggler and coworkers21,68, which corresponds to 0.78 and 1.32 M% molar polarization and 0.078 and 0.132 mmol% molecular polarization, respectively. In our work, we achieved 17.4% 13C polarization for 100 mM concentration of 1 mL 1-13C-EP-d6 solution, corresponding to 1.74 M% molar polarization and 1.74 mmol%. Hence, PHIP-SAH is approaching the levels of dDNP but is yet about one order magnitude short of doing so.
When this manuscript was in preparation, remarkable polarization levels for pyruvate polarized at high magnetic fields were reported, although limited to the low volumes in 5 mm NMR tubes21. As reported in this work, we repeated our experiments in acetone and observed spontaneous premature dissociation of the sidearm after hydrogenation (Fig. S6, SI). We measured the content of water in acetone to be about 30 mM which should be removed before the experiment. Hence, special care should be taken to use water-free solvents. Note, that the use of molecular sieves is not advisable as they are weakly basic and catalyze the aldol condensation69. It seems that one should use molecular sieves only for a short time, several hours or a day before the experiment, or instead use powdered boric acid anhydride70. Further analysis of PHIP-SAH in acetone was not attempted in our study.
Conclusion
We performed studies to optimize the polarization level of 1-13C-pyruvate using PHIP-SAH and we present corresponding experimental procedures. We found that RF-SOT offers more polarization than MFC, in theory, and we state that 1-13C-VP-d6 is the most promising precursor to generate hyperpolarized 1-13C-pyruvate with PHIP. Specifically, an ESOTHERIC sequence applied to 1-13C-EP-d6 promises a 100% polarization for homogeneous B0 and B1 fields. Still, the need for metabolic MRI, high polarization of large amounts, are not easy to address at once. Here, we were hydrogenating and polarizing 1 mL of 100 mM of 1-13C-VP-h6, -d3, -d6 and achieved 1%, 5%, 6.5% 13C-polarization with 22 s hydrogenation, and 17.4% for 1-13C-VP-d6 with only 7 s of pH2 hydrogenation time. Performing the experiments at a 9.4 T WB NMR appears feasible but has some drawbacks including shorter relaxation times at higher fields. Our method could be improved further using specialized benchtop NMRs. Such benchtops should also provide the ability to polarize 1–5 mL samples, RF coils should provide less than ± 5% deviation from the nominal angle for the entire sample. Such benchtops are not currently available. Although each step of the process demonstrated here could be further optimized, the concept is easy to implement in almost every laboratory. With the appearance of more dedicated PHIP-SAH polarizers, it will be a valuable competitor to dDNP and a useful tool in a versatile NMR toolkit.
Data availability
Data generated or analyzed during this study with a short description are included in this published article (raw_data.zip). The MOIN spin library34, open-source scripts to simulate SOT performance, and necessary processed experimental data are included in the separate supplementary file (MOINlib.zip).
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Acknowledgements
We acknowledge funding from German Federal Ministry of Education and Research (BMBF) within the framework of the e:Med research and funding concept (01ZX1915C), DFG (PR 1868/3-1, HO-4604/2-2, HO-4604/3, GRK2154-2019, EXC2167, FOR5042, SFB1479, TRR287). MOIN CC was founded by a grant from the European Regional Development Fund (ERDF) and the Zukunftsprogramm Wirtschaft of Schleswig-Holstein (Project no. 122-09-053).
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A.N.P., J.B.H.: conceptualization, writing—original draft. A.B., R.H., and T.S.: VP synthesis. A.N.P., F.E. investigation of EP hyperpolarization. A.N.P., R.H., and J.B.H.: supervision, funding acquisition. All authors contributed to discussions and interpretation of the results and have approved the final version of the manuscript. We thank our DAAD-rise student Aidan Sirbu for the discussion and reading.
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Pravdivtsev, A.N., Brahms, A., Ellermann, F. et al. Parahydrogen-induced polarization and spin order transfer in ethyl pyruvate at high magnetic fields. Sci Rep 12, 19361 (2022). https://doi.org/10.1038/s41598-022-22347-1
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DOI: https://doi.org/10.1038/s41598-022-22347-1
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