Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constructs in Figs. 2c, f and Supplementary Fig. 9 are therefore no longer valid. However the overall conclusion that step-wise control over gene expression levels using the miSFIT constructs remains unaffected by these errors. Updated versions of Fig. 2 and Supplementary Fig. 9 are presented in the accompanying Addendum.
Addendum to: Nature Communications https://doi.org/10.1038/s41467-019-08777-y, published online 18 February 2019.
Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3′UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constructs in Fig. 2c, f and Supplementary Fig. 9 are therefore no longer valid. However the overall conclusion that the miSFIT system allows step-wise control over gene expression levels remains unaffected by these errors. Updated versions of Fig. 2 and Supplementary Fig. 9 are presented below as Figs. 1 and 2 respectively.
Fig. 1
Fig. 2
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Michaels, Y.S., Barnkob, M.B., Barbosa, H. et al. Addendum: Precise tuning of gene expression levels in mammalian cells. Nat Commun 10, 2622 (2019). https://doi.org/10.1038/s41467-019-10615-0
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DOI: https://doi.org/10.1038/s41467-019-10615-0
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