CRISPR-Cas9 genome editing

Definition

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the double strand break can result in insertion or deletion mutations, while repair pathways can be engineered to introduce specific point mutations or insertions.

Latest Research and Reviews

  • Research | | open

    Bicuspid aortic valve (BAV) is the most common human congenital cardiovascular malformation. Here, the authors perform a genome-wide association study for BAV and identify risk variants in the gene region of cardiac-specific transcription factor GATA4 and implicate GATA4 in heart valve development.

    • Bo Yang
    • , Wei Zhou
    • , Jiao Jiao
    • , Jonas B. Nielsen
    • , Michael R. Mathis
    • , Mahyar Heydarpour
    • , Guillaume Lettre
    • , Lasse Folkersen
    • , Siddharth Prakash
    • , Claudia Schurmann
    • , Lars Fritsche
    • , Gregory A. Farnum
    • , Maoxuan Lin
    • , Mohammad Othman
    • , Whitney Hornsby
    • , Anisa Driscoll
    • , Alexandra Levasseur
    • , Marc Thomas
    • , Linda Farhat
    • , Marie-Pierre Dubé
    • , Eric M. Isselbacher
    • , Anders Franco-Cereceda
    • , Dong-chuan Guo
    • , Erwin P. Bottinger
    • , G. Michael Deeb
    • , Anna Booher
    • , Sachin Kheterpal
    • , Y. Eugene Chen
    • , Hyun Min Kang
    • , Jacob Kitzman
    • , Heather J. Cordell
    • , Bernard D. Keavney
    • , Judith A. Goodship
    • , Santhi K. Ganesh
    • , Gonçalo Abecasis
    • , Kim A. Eagle
    • , Alan P. Boyle
    • , Ruth J. F. Loos
    • , Per Eriksson
    • , Jean-Claude Tardif
    • , Chad M. Brummett
    • , Dianna M. Milewicz
    • , Simon C. Body
    •  & Cristen J. Willer
  • Research | | open

    CRISPR-Cas9 has been utilized to screen the genome in loss-of-function studies to identify genetic interactions. Here the authors compare catalytically active and dead Cas9 and observe different off-target effects in a screen for essential genes.

    • Joseph Rosenbluh
    • , Han Xu
    • , William Harrington
    • , Stanley Gill
    • , Xiaoxing Wang
    • , Francisca Vazquez
    • , David E. Root
    • , Aviad Tsherniak
    •  & William C. Hahn
  • Research | | open

    While CRISPR-Cas9 is a powerful technology, it’s in vivo application can be limited by unwanted off-target editing events. Here the authors present SLiCES, a self-limiting Cas9 circuit to enhance editing by preventing residual nuclease activity.

    • Gianluca Petris
    • , Antonio Casini
    • , Claudia Montagna
    • , Francesca Lorenzin
    • , Davide Prandi
    • , Alessandro Romanel
    • , Jacopo Zasso
    • , Luciano Conti
    • , Francesca Demichelis
    •  & Anna Cereseto
  • Research | | open

    Double-strand breaks are a major DNA lesion that can occur by endogenous and exogenous processes. Here the authors present BLISS—Breaks Labelling In Situ and Sequencing—to map breaks across the genome.

    • Winston X. Yan
    • , Reza Mirzazadeh
    • , Silvano Garnerone
    • , David Scott
    • , Martin W. Schneider
    • , Tomasz Kallas
    • , Joaquin Custodio
    • , Erik Wernersson
    • , Yinqing Li
    • , Linyi Gao
    • , Yana Federova
    • , Bernd Zetsche
    • , Feng Zhang
    • , Magda Bienko
    •  & Nicola Crosetto

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