Abstract
Ubiquitination of epidermal growth factor receptor (EGFR) is required for downregulation of the receptor by endocytosis. Impairment of this pathway results in constitutively active EGFR, which is associated with carcinogenesis, particularly in lung cancer. We previously demonstrated that the deubiquitinating enzyme ubiquitin-specific protease 2a (USP2a) has oncogenic properties. Here, we show a new role for USP2a as a regulator of EGFR endocytosis. USP2a localizes to early endosomes and associates with EGFR, stabilizing the receptor, which retains active downstream signaling. HeLa cells transiently expressing catalytically active, but not mutant (MUT), USP2a show increased plasma membrane-localized EGFR, as well as decreased internalized and ubiquitinated EGFR. Conversely, USP2a silencing reverses this phenotype. Importantly, USP2a prevents the degradation of MUT in addition to wild-type EGFR. Finally, we observed that USP2a and EGFR proteins are coordinately overexpressed in non-small cell lung cancers. Taken together, our data indicate that USP2a antagonizes EGFR endocytosis and thus amplifies signaling activity from the receptor. Our findings suggest that regulation of deubiquitination could be exploited therapeutically in cancers overexpressing EGFR.
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Abbreviations
- CHC:
-
clathrin heavy chain
- DUB:
-
deubiquitinating enzyme
- EEA1:
-
early endosomal antigen
- EGFR:
-
epidermal growth factor receptor
- WT:
-
wild type
- MUT:
-
mutant
- RTK:
-
receptor tyrosine kinase
- TfR:
-
transferrin receptor
- USP2:
-
ubiquitin-specific protease 2
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Acknowledgements
We thank Tomas Kirchhausen for AMSH constructs, Mihee Ji and Erik McCauley for technical assistance, Jane Hayward for help with preparation of the figures, Giulio Draetta and Richard J Flavin for critical reading of the manuscript. This work was supported by the National Cancer Institute (RO1CA131945, PO1CA89021, P50 CA90381 and the Prostate Cancer Foundation). SMZ was supported by CAPES (no. 3665/10-0), Brazil.
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Liu, Z., Zanata, S., Kim, J. et al. The ubiquitin-specific protease USP2a prevents endocytosis-mediated EGFR degradation. Oncogene 32, 1660–1669 (2013). https://doi.org/10.1038/onc.2012.188
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DOI: https://doi.org/10.1038/onc.2012.188
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